Kang W.-M.,Peking Union Medical College |
Meng Q.-B.,First Hospital of Wuhan City |
Yu J.-C.,Peking Union Medical College |
Ma Z.-Q.,Peking Union Medical College |
Li Z.-T.,Peking Union Medical College
World Journal of Gastroenterology | Year: 2015
AIM: To characterize patterns of gastric cancer recurrence and patient survival and to identify predictors of early recurrence after surgery. METHODS: Clinicopathological data for 417 consecutive patients who underwent curative resection for gastric cancer were retrospectively analyzed. Tumor and node status was reclassified according to the 7th edition of the American Joint Committee on Cancer tumor-node-metastasis classification for carcinoma of the stomach. Survival data came from both the patients' follow-up records and telephone followups. Recurrent gastric cancer was diagnosed based on clinical imaging, gastroscopy with biopsy, and/or cytological examination of ascites, or intraoperative findings in patients who underwent reoperation. Predictors of early recurrence were compared in patients with pT1 and pT2-4a stage tumors. Pearson's χ2 test and Fisher's exact test were used to compare differences between categorical variables. Survival curves were constructed using the Kaplan-Meier method and compared via the log-rank test. Variables identified as potentially important for early recurrence using univariate analysis were determined by multivariate logistic regression analysis. RESULTS: Of 417 gastric cancer patients, 80 (19.2%) were diagnosed with early gastric cancer and the remaining 337 (80.8%) were diagnosed with locally advanced gastric cancer. After a median follow-up period of 56 mo, 194 patients (46.5%) experienced recurrence. The mean time from curative surgery to recurrence in these 194 patients was 24 ± 18 mo (range, 1-84 mo). Additionally, of these 194 patients, 129 (66.5%) experienced recurrence within 2 years after surgery. There was no significant difference in recurrence patterns between early and late recurrence (P < 0.05 each). For pT1 stage gastric cancer, tumor size (P = 0.011) and pN stage (P = 0.048) were associated with early recurrence of gastric tumors. Patient age, pT stage, pN stage, Lauren histotype, lymphovascular invasion, intraoperative chemotherapy, and postoperative chemotherapy were independent predictors of early recurrence in patients with pT2-4a stage gastric cancer (P < 0.05 each). CONCLUSION: Age, pT stage, pN stage, Lauren histotype, lymphovascular invasion, intraoperative chemotherapy, and postoperative chemotherapy are independent factors influencing early recurrence of pT2-4a stage gastric cancer. © The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
Meng Q.-B.,Peking Union Medical College |
Meng Q.-B.,First Hospital of Wuhan City |
Kang W.-M.,Peking Union Medical College |
Yu J.-C.,Peking Union Medical College |
And 5 more authors.
PLoS ONE | Year: 2015
Eukaryotic translation initiation factor 5A2 (EIF5A2) plays an important role in tumor progression and prognosis evaluation. However, little information is available about its potential role in gastric cancer. This study aimed to investigate the function of EIF5A2 in tumor progression and its potential mechanisms. EIF5A2 expression was measured in human gastric cancer cell lines, the immortalized gastric mucosal epithelial cell line (GES-1) and human gastric cancer tissues and knocked down by RNA interference or upregulated by EIF5A2 plasmid transfection. Cell proliferation, migration and invasion were assessed in vitro . The downstream targets of EIF5A2 were examined by western blotting. EIF5A2 and its potential target metastasis-associated protein 1 (MTA1) expression were examined in 160 pairs of human gastric cancer and adjacent non-tumor specimens using immunohistochemistry (IHC) staining, and its correlation with clinicopathological features and survival was investigated. Knockdown of EIF5A2 or MTA1 caused an apparent suppression of HGC27 cell proliferation, migration and invasion. After knockdown of EIF5A2 in HGC27 cells, E-cadherin levels were upregulated and vimentin, cyclin D1, cyclin D3, C-MYC and MTA1 levels were downregulated. Upregulation of EIF5A2 in MKN45 cells resulted in the converse. IHC results showed a positive correlation between EIF5A2 and MTA1 expression in gastric cancers (P<0.001). Both EIF5A2 and MTA1 overexpression were correlated with pT stage (P=0.018 and P=0.042), pN stage (P=0.037 and P=0.020) and lymphovascular invasion (P=0.016 and P=0.044). EIF5A2 or MTA1 overexpression was significantly associated with poor overall survival and disease-free survival (All P<0.05). Multivariate analyses identified EIF5A2 as an independent predictor for both overall survival (P=0.012) and disease-free survival (P=0.008) in gastric cancer patients. Our findings indicate that EIF5A2 upregulation plays an important oncogenic role in gastric cancer. EIF5A2 may represent a new predictor for poor survival and is a potential therapeutic target for gastric cancer. © 2015 Meng et al.
Sanusi T.,Huazhong University of Science and Technology |
Li Y.,Zhongshan Hospital |
Sun L.,First Hospital of Wuhan City |
Wang C.,Huazhong University of Science and Technology |
And 2 more authors.
Dermatology | Year: 2015
Background: Eccrine angiomatous hamartoma (EAH) is a rare benign cutaneous tumor characterized by the proliferation of eccrine glands and capillaries. Objective: The aim of this study was to summarize the clinicopathological characteristics of EAH. Methods: A retrospective chart review was performed on all patients diagnosed with EAH from 1977 to 2012 in the Union Hospital, Wuhan, P.R. China, and the clinicopathological features were compared with the cases reported in the literature. Results: A total of 26 patients with EAH were identified. The male:female ratio was 1.2:1. EAH most commonly presents as a solitary (80.8%) plaque (50.0%) on the lower extremities (61.5%). Most patients presented with hyperhidrosis localizing to the lesion. Although most patients did not have major pain or anatomic deformity, one patient had severe pain as well as difficulty walking and moving, necessitating leg amputation. The histopathological findings showed typical features of EAH. Conclusion: EAH is a rare but characteristically benign skin hamartomatous condition. In rare occasions it can be associated with severe structural and functional impairment. © 2015 S. Karger AG, Basel.
Mei J.H.,First Hospital of Wuhan City
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2010
To study the influence of neuro-psychological factors on the effect of acupuncture in the treatment of Bell's palsy and the overall prognosis in patients. Fifty patients with Bell's palsy were randomized into the treatment group and the control group, and they were treated with manipulated and non-manipulated acupuncture, respectively. Scorings by subjective perceptive scale of acupuncture, Cartel personality test, and Hamilton Anxiety Scale were performed and the curative effect was assessed according House-Brackmann grading standards. The total effective rate of acupuncture was 78.0% (39/50), and that of manipulated acupuncture was better than that of non-manipulated acupuncture [89.2% (25/28) vs. 63.6% (14/22), P < 0.01]. Visual analogue scoring for perception of "Deqi", evaluated either by patients or by doctors, showed that the scores was higher in the treatment group than in the control group (P < 0.01). Cartel personality test (16PF) found that patients with personality factors of sociability, intellectuality, excitability, braveness, and independence were capable of getting "Deqi" more easily, there existed a significant correlation between personality factors and curative effect. By Hamilton Anxiety Scale scoring, 92.0% (46/50) of the patients were found being in an anxiety state, and the efficacy of treatment was negatively correlated with the degree of anxiety (r = -0.9491, P < 0.05). Neuro-psychological factors put great influence on the efficacy of treatment for Bell's palsy, multiple measures, such as drug-therapy, acupuncture, psychological intervention, rehabilitation therapy, etc., should be taken in combination for improving patients' prognosis.
Lu M.,First Hospital of Wuhan City |
Gao Y.,First Hospital of Wuhan City |
Xiong Q.,First Hospital of Wuhan City
Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology | Year: 2011
Background: To explore the roles of human papilloma virus (HPV) and herpes simplex virus (HSV) in pathogenesis of pterygia are very important for provide more basis for the study of pterygia mechanisms. Objective The aim of the study was to detect the expression of HSV and HPV in pterygia. Methods: A total of 68 specimens of pterygia and specimens of normal conjunction tissue were obtained during the operation. The expression of HSV DNA and HPV DNA in pterygia specimens and conjunctival tissue were detected and compared by real time fluorescence quantitative polymerase chain reaction (PCR). Written informed consent was obtained from each patient before the any medical procedure related to this study. Results: Real time fluorescence quantitative PCR showed that HPV DNA was detected in 12 (17.65%) pterygia specimens, and HSV DNA was found in 15 (22.06%) pterygia specimens; however them were detected in 6(8.82%) and 1(1.47%) in conjunctival tissue respectively. Significant differences were found in the expression both HSV DNA and HPV DNA between pterygium tissue and conjunctival tissue (HPV: χ 2=10.291, P < 0.05; HSV: χ 2=4.561, P < 0.05). The co-expression of HSV DNA and HPV DNA was seen in 5 (7.35%) pterygium specimens, but no any co-expression of HSV DNA and HPV DNA was in conjunctival specimen. Conclusion: HSV and HPV may participate in the pathogenesis and development of pterygium. The detection of virus DNA can offer an experiment evidence of HSV and HPV in pterygium generation.
Wang F.,Huazhong University of Science and Technology |
Xu C.-Q.,Huazhong University of Science and Technology |
He Q.,Beijing Hospital |
Cai J.-P.,Beijing Hospital |
And 55 more authors.
Nature Genetics | Year: 2011
Coronary artery disease (CAD) causes more than 700,000 deaths each year in China. Previous genome-wide association studies (GWAS) in populations of European ancestry identified several genetic loci for CAD, but no such study has yet been reported in the Chinese population. Here we report a three-stage GWAS in the Chinese Han population. We identified a new association between rs6903956 in a putative gene denoted as C6orf105 on chromosome 6p24.1 and CAD (P = 5.00-10'3, stage 2 validation; P = 3.00 - 103, P = 1.19 - 10 '8 and P = 4.00 - 10 '3 in three independent stage 3 replication populations; P = 4.87 - 10'12, odds ratio = 1.51 in the combined population). The minor risk allele A of rs6903956 is associated with decreased C6orf105 mRNA expression. We report the first GWAS for CAD in the Chinese Han population and identify a SNP, rs6903956, in C6orf105 associated with susceptibility to CAD in this population. © 2011 Nature America, Inc. All rights reserved.
Huang Q.-S.,First Hospital of Wuhan City |
Yang J.,First Hospital of Wuhan City |
Cao J.,First Hospital of Wuhan City |
Zhou X.-Y.,First Hospital of Wuhan City
Journal of Clinical Dermatology | Year: 2013
A case of Calcium dobesilate-induced toxic epidermal necrolysis (TEN) is reported. A 56-year-old woman had chronic renal failure. The patient was administered with calcium dobesilate for three times. Generalized painful erythemas, vesicles and fever arose a few hours after each calcium dobesilate administration. After discontinuation of calcium dobesilate and treatments with glucocorticoids, immunoglobulin and symptomatic treatment, rashes disappeared and body temperature became normal.
Fu Q.,Huazhong University of Science and Technology |
Liu J.,Huazhong University of Science and Technology |
Zhang C.,Huazhong University of Science and Technology |
Zhou Y.,Huazhong University of Science and Technology |
And 2 more authors.
Journal of Huazhong University of Science and Technology - Medical Science | Year: 2010
The chemical constituents of fistular onion stalk obtained by supercritical CO2 extraction were separated and purified by silica gel and sephadex LH-20 gel column chromatography and the preparative TLC method and four flavonoids were obtained. On the basis of the spectral data, they were structurally identified as (+)-catechin, (-)-epicatechin, astragalin, and 3-O-β-D(2-O-β-D-glucopyranosyl)-glucopyranosides of kaempferol. © 2010 Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg.
Yao R.,Huazhong University of Science and Technology |
Ma Y.,Huazhong University of Science and Technology |
Du Y.,Zhengzhou University |
Liao M.,Huazhong University of Science and Technology |
And 7 more authors.
Cellular and Molecular Immunology | Year: 2011
MicroRNAs (miRNAs) are a novel class of small, non-coding RNAs that play a significant role in both inflammatory and cardiovascular diseases. Immune cells, especially T helper (Th) cells, are critical in the development of atherosclerosis and the onset of acute coronary syndrome (ACS). To assess whether inflammation-related miRNAs (such as miR-155, 146a, 21, 125a-5p, 125b, 31) are involved in the imbalance of Th cell subsets in patients with ACS, we measured the expression of related miRNAs in patients with acute myocardial infarction (AMI), unstable angina (UA), stable angina (SA) and chest pain syndrome (CPS); analyzed the relationship between miRNA expression and the frequency of Th cell subsets; and observed the co-expression of miR-155 and IL-17A in peripheral blood mononuclear cells (PBMCs) of patients with ACS. The results showed that the expression of miR-155 in the PBMCs of patients with ACS was decreased by approximately 60%, while the expression of both miR-21 and miR-146a was increased by approximately twofold. The expression patterns of miRNAs in plasma correlated with those in PBMCs, except for miR-21, which was increased by approximately sixfold in the AMI group and showed no significant difference between the UA group and the CPS group. We also found that the expression of miR-155 inversely correlated with the frequency of Th17 cells (r=0.896, P<0.01) and that miR-155 was co-expressed with IL-17A in patients with ACS. In conclusion, our study revealed the expression patterns of inflammation-related miRNAs in patients with ACS and found that miR-155 may be associated with Th17 cell differentiation. © 2011 CSI and USTC. All rights reserved.
PubMed | First Hospital of Wuhan City
Type: Journal Article | Journal: Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine | Year: 2010
To study the influence of neuro-psychological factors on the effect of acupuncture in the treatment of Bells palsy and the overall prognosis in patients.Fifty patients with Bells palsy were randomized into the treatment group and the control group, and they were treated with manipulated and non-manipulated acupuncture, respectively. Scorings by subjective perceptive scale of acupuncture, Cartel personality test, and Hamilton Anxiety Scale were performed and the curative effect was assessed according House-Brackmann grading standards.The total effective rate of acupuncture was 78.0% (39/50), and that of manipulated acupuncture was better than that of non-manipulated acupuncture [89.2% (25/28) vs. 63.6% (14/22), P < 0.01]. Visual analogue scoring for perception of Deqi, evaluated either by patients or by doctors, showed that the scores was higher in the treatment group than in the control group (P < 0.01). Cartel personality test (16PF) found that patients with personality factors of sociability, intellectuality, excitability, braveness, and independence were capable of getting Deqi more easily, there existed a significant correlation between personality factors and curative effect. By Hamilton Anxiety Scale scoring, 92.0% (46/50) of the patients were found being in an anxiety state, and the efficacy of treatment was negatively correlated with the degree of anxiety (r = -0.9491, P < 0.05).Neuro-psychological factors put great influence on the efficacy of treatment for Bells palsy, multiple measures, such as drug-therapy, acupuncture, psychological intervention, rehabilitation therapy, etc., should be taken in combination for improving patients prognosis.