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Yang C.,Zhongshan Hospital of Hubei Province | Hu D.-H.,Zhongshan Hospital of Hubei Province | Feng Y.,First Hospital of Wuhan
Molecular Medicine Reports

The aim of the present study was to investigate the chemical composition of the essential oil of Artemisia vestita and to determine the antibacterial activity of the essential oil and its two major components, grandisol and 1,8-cineole, against certain respiratory infection-causing bacterial strains, in vitro and in vivo. The chemical composition of the essential oil was analyzed using gas chromatography-mass spectrometry. A micro-well dilution method was used to determine the minimum inhibition concentration (MIC) values of the essential oil and its major constituents. A model of Streptococcus pyogenes infection in mice was used to determine its in vivo activities. Lung and blood samples were obtained to assess bacterial cell counts. Toxicity evaluation of the essential oil and its components was completed by performing biochemical analysis of the serum, particularly monitoring aspartate transaminase, alanine transaminase, urea and creatinine. The essential oil exhibited potent antibacterial activity, whereas the two major constituents were less potent. The essential oil exhibited MIC values between 20 and 80 μg/ml, while the values of the two constituents were between 130 and 200 μg/ml. Scanning electron microscopy results demonstrated that the essential oil inhibited biofilm formation and altered its architecture. Survival curves indicated that the essential oil led to a reduction in the viability of different bacteria. The essential oil also induced significant leakage of potassium ions from S. pyogenes. The essential oil (100 μg/mouse) and grandisol (135 μg/mouse) significantly reduced the number of viable bacterial cells in the lungs (P<0.01). However, intake of 100 μg/mouse of essential oil or grandisol 135 μg/mouse once or twice each day for 9 days did not produce any toxic effects in the mice. In conclusion, the in vitro and in vivo results suggested that the essential oil of A. vestita and one of its major constituents, grandisol, can significantly inhibit the growth of different bacterial strains. Source

Chen H.,Huazhong University of Science and Technology | Zheng C.,First Hospital of Wuhan | Zhang X.,Huazhong University of Science and Technology | Li J.,Wuhan University | And 2 more authors.

Apelin, a newly identified bioactive adipokine, has been found to play important roles in multiple diseases, including diabetes, hypertension and cardiovascular diseases with unclear molecular mechanisms. The present study aimed to investigate the effects of apelin on endoplasmic reticulum (ER) stress in the pancreas of Akita mice, a well-established type 1 diabetic model. Apelin-13 (400 pmol/kg) was injected from tail vein for 10 weeks. The physiological characters of experimental animals were evaluated, pancreatic islet morphology and insulin content were assessed by immunohistochemistry, and ER stress markers in the pancreas were examined by Western blots. Our results indicate apelin treatment significantly ameliorates diabetes-induced reduction in pancreatic islet mass and insulin content. Further studies suggested apelin treatment alleviates ER stress by inhibiting the diabetes induced up-regulation of PERK and IRE1α and chaperones (GRP78, calnexin and Hsp70) levels in Akita mice. We also demonstrated that apelin treatment normalizes the diabetes induced alteration of AKT and ERK activations in the pancreas of Akita mice. Taken together, these results suggest a novel physiological role of apelin in alleviating ER stress in the pancreas of type 1 diabetes. © 2011 Elsevier Inc. Source

Wu X.,Huazhong University of Science and Technology | Liu D.,Huazhong University of Science and Technology | Tao D.,Fifth Hospital of Wuhan | Xiang W.,First Hospital of Wuhan | And 7 more authors.
Molecular Cancer Therapeutics

People who develop bladder cancer frequently succumb to the intractable disease. Current treatment strategies are limited presumably due to the underlying molecular complexity and insufficient comprehension. Therefore, exploration of new therapeutic targets in bladder cancer remains necessary. Here, we identify that bromodomain-4 protein (BRD4), an important epigenome reader of bromodomain and extraterminal domain (BET) family member, is a key upstream regulator of enhancer of zeste homologue 2 (EZH2), and represents a novel therapeutic target in bladder cancer. We found that BRD4 was significantly overexpressed in bladder cancer cells and tissues. Inhibition of BRD4 decreased bladder cancer cell proliferation concomitantly with the accumulation of cell apoptosis in vitro and suppressed tumor growth in vivo. We further found that suppression of BRD4 decreased the mRNA and protein levels of EZH2, which was reversed by ectopic expression of C-MYC. In particular, individual silencing of BRD4 using shRNA or the BET inhibitor JQ1 strikingly diminished the recruitment of C-MYC to EZH2 promoter in bladder cancer. Briefly, our research reveals that BRD4 positively regulates EZH2 transcription through upregulation of C-MYC, and is a novel promising target for pharmacologic treatment in transcriptional program intervention against this intractable disease. © 2016 AACR. Source

Yao S.,Wuhan University | Zhou J.,First Hospital of Wuhan | Li Z.,Wuhan University
Journal of Craniofacial Surgery

Background: The appearance and dysfunction of deformities may cause psychologic disorders in patients. The aim of this study was to assess the psychologic health status of patients undergoing orthognathic surgery and its relationship with demographic characteristics, social activityof the individuals, and severity of maxillofacial deformity. Methods: As a tool for assessing the psychologic health status, the Symptom Checklist-90 (SCL-90) questionnaire was used on 318 patients undergoing orthognathic surgery at the Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, China from January 2006 to November 2012. t-Test was used to compare the psychologic health status between the 318 patients and 200 healthy volunteers. Variance analysis (analysis of variance) was applied for assessing the relationship between the psychologic health status and the variables of the study. Results: The SCL-90 total score of the patients undergoing orthognathic surgery was higher than that of the healthy volunteers (P < 0.05). Results of the t-test revealed statistically significant difference (P < 0.05) in the symptom dimensions of obsessive-compulsive, interpersonal sensitivity, depression, and paranoid ideation. An association between sex and the SCL-90 total score was observed (F = 11.293; P = 0.001 and P <0.05). The patients who had regular work presented better psychological health status than did the patients who had no regular work (F = 8.008; P = 0.005 and P < 0.05). The patients who received comfort from family and relatives presented better psychological health status than did the patients who did not receive such help (F = 10.064; P = 0.002 and P < 0.05). The patients who received economic help from family and relatives presented better psychological health status than did the patients who did not receive such help (F = 9.789; P = 0.002 and P < 0.05). An association was found between severity of deformity and psychologic health (F = 6.394; P = 0.002 and P < 0.05). Conclusions: Patients with jaw deformity have significant psychologic problems and their psychologic health is affected by demographic characteristics, social activity of individuals, and severity of maxillofacial deformity. Copyright © 2014 by Mutaz B. Habal, MD. Source

Liao Y.-H.,Huazhong University of Science and Technology | Xia N.,Huazhong University of Science and Technology | Zhou S.-F.,Huazhong University of Science and Technology | Tang T.-T.,Huazhong University of Science and Technology | And 12 more authors.
Journal of the American College of Cardiology

Objectives: This study tested whether interleukin (IL)-17A is involved in the pathogenesis of mouse myocardial ischemia/reperfusion (I/R) injury and investigated the mechanisms. Background: Inflammatory processes play a major role in myocardial I/R injury. We recently identified IL-17A as an important cytokine in inflammatory cardiovascular diseases such as atherosclerosis and viral myocarditis. However, its role in myocardial I/R injury remains unknown. Methods: The involvement of IL-17A was assessed in functional assays in mouse myocardial I/R injury by neutralization/repletion or genetic deficiency of IL-17A, and its mechanism on cardiomyocyte apoptosis and neutrophil infiltration were further studied in vivo and in vitro. Results: Interleukin-17A was elevated after murine left coronary artery ligation and reperfusion. Intracellular cytokine staining revealed that γδT lymphocytes but not CD4+ helper T cells were a major source of IL-17A. AntiIL-17A monoclonal antibody treatment or IL-17A knockout markedly ameliorated I/R injury, as demonstrated by reduced infarct size, reduced cardiac troponin T levels, and improved cardiac function. This improvement was associated with a reduction in cardiomyocyte apoptosis and neutrophil infiltration. In contrast, repletion of exogenous IL-17A induced the opposite effect. In vitro study showed that IL-17A mediated cardiomyocyte apoptosis through regulating the Bax/Bcl-2 ratio, induced CXC chemokine-mediated neutrophil migration and promoted neutrophil-endothelial cell adherence through induction of endothelial cell E-selectin and inter-cellular adhesion molecule-1 expression. Conclusions: IL-17A mainly produced by γδT cells plays a pathogenic role in myocardial I/R injury by inducing cardiomyocyte apoptosis and neutrophil infiltration. © 2012 American College of Cardiology Foundation. Source

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