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Xie F.,University of Veterinary and Animal Sciences | Lei L.,University of Veterinary and Animal Sciences | Du C.,University of Veterinary and Animal Sciences | Li S.,Shanghai Entry Exit Inspection and Quarantine Bureau | And 2 more authors.
FEMS Microbiology Letters | Year: 2010

The limited information on the genetic differences among the 15 currently known serotypes of Actinobacillus pleuropneumoniae has significantly hampered the development of typing-based diagnostic methods and multivalent vaccines. In this study, we compared the genomic differences between A. pleuropneumoniae strains CVCC259 (serotype 1) and CVCC261 (serotype 3) by representational difference analysis. Of the eight differential DNA sequences in the CVCC259 strain and 11 differential DNA sequences in the CVCC261 strain that we identified, seven represent known virulent genes, 10 encode putative proteins, and two encode hypothetical proteins. We also investigated the distribution of these 19 sequences among the 15 serotypes, and each serotype showed a different distribution pattern. The autotransporter adhesin occurred as a novel putative virulence factor in serotypes 1, 5, 7, 8, 9, and 11. Notably, the presence of wzm and wzt in serotypes 1, 9, and 11 and the diverse distribution of wzz and wzy in the other serotypes suggest the presence of different O-antigen biosynthesis pathways among serotypes. The information on the differential distribution of these DNA sequences in the 15 serotypes of A. pleuropneumoniae may contribute to future research on the pathogenic mechanisms of different serotypes, typing-based diagnosis methods, and multivalent vaccines. © 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

Liang X.-N.,Capital Medical University | Guo R.-J.,Capital Medical University | Li S.,Capital Medical University | Zheng Z.-M.,First Hospital of Shijiazhuang City | And 2 more authors.
European Review for Medical and Pharmacological Sciences | Year: 2014

OBJECTIVE: The common clinical techniques used for examining thyroid tumors include palpat ion, imaging, immunoassays and tissue biopsy. Ultrasonography is easy, non-invasive, non-radioactive and highly reproducible imaging technique; however, due to the disease polytropism, diagnosis may become difficult sometimes. Ultrasound elastography, particularly acoustic radiation force impulse (ARFI) imaging and contrast-enhanced ultrasonography (CEUS) have been successfully used to diagnose the thyroid tumors. The objective of this retrospective study was to analyze and compare the solid thyroid nodules imaged by high-frequency ultrasonography (HFUS), ARFI imaging, and CEUS. PATIENTS AND METHODS: For this purpose, images of the 80 solid thyroid nodules (58 benign and 22 malignant) with surgical pathology were obtained and data were compared using binary logistic regression analysis. RESULTS: Morphology (p < 0.001), and internal calcification (p = 0.007) were statistically different. The mean shear wave velocity (SWV) measured by ARFI was significantly different (p = 0.029). Three sets of comparison on CEUS (p = 0.019) and time to peak (TTP) of CEUS were significantly different (p = 0.001). The logistic regression analysis indicated that the morphology, mean SWV of ARFI and TTP were independent risk factors for malignancy. The diagnostic accuracy for solid thyroid nodules was 85.1% (68/80) and the area under the receiver operating characteristic (ROC) curve was 0.945±0.033. CONCLUSIONS: Logistic regression analysis can effectively screen significant parameters for the differential diagnosis of solid thyroid nodules imaged by ultrasonography.

Liu F.,Central South University | Liao F.,Central South University | Li W.,First Hospital of Changsha | Han Y.,First Hospital of Shijiazhuang City | Liao D.,Central South University
Molecular Medicine Reports | Year: 2014

Previous studies have demonstrated that progesterone has neuroprotective effects in the central nervous system (CNS) following traumatic brain injury (TBI). Numerous cellular mechanisms have been reported to be important in the neuroprotective effects of progesterone, including the reduction of edema, inflammation and apoptosis, and the inhibition of oxidative stress. However, the effect of progesterone on neuronal protection following TBI remains unclear. The present study aimed to investigate the effects of progesterone on the expression of Nogo-A, an inhibitor of axonal growth, glial fibrillary acidic protein (GFAP), a main component of the glial scar and growth-associated protein-43 (GAP-43), a signaling molecule in neuronal growth in TBI rats. The TBI model was produced by the weight drop method. In total, 75 rats were assigned to three groups: the sham group, TBI group with vehicle treatment and TBI group with progesterone treatment. The protein expression of Nogo-A, GFAP and GAP-43 in the cortex and the hippocampus was examined by immuno-cytochemistry. TBI rats significantly increased the expression of Nogo-A, GFAP, and GAP-43 at 1, 3, 7 and 14 days post-injury. Progesterone significantly decreased the expression of Nogo-A and GFAP, and upregulated the GAP-43 protein. Our findings suggested that progesterone promotes neuroprotection following TBI by inhibiting the expression of Nogo-A and GFAP, and increasing GAP-43 expression.

Wang H.,First Hospital of Shijiazhuang City | Zheng Z.-M.,First Hospital of Shijiazhuang and Organ Transplantation Committee | Gao B.-L.,First Hospital of Shijiazhuang and Organ Transplantation Committee
Evidence-based Complementary and Alternative Medicine | Year: 2014

Purpose. To investigate the antiatherosclerotic effect of Guanxinkang (GXK) decoction on the apoptosis, mitochondrial membrane potential (MMP), and endoplasmic reticulum stress (ERS) of human umbilical vein endothelial cells (HUVEC) pretreated with homocysteinemia (HCY). Materials and Methods. HUVEC were randomly divided into 5 groups: (1) blank control group (control), (2) model control group (model), (3) GXK low dose group, (4) GXK medium dose group, and (5) GXK high dose group. For the three GXK groups, HCY was given to reach the concentration of 3.0 mmol/L after HUVEC had been incubated with rabbit serum containing GXK for two hours. At 3, 6, 12, and 24 h after HCY had been incubated with the cells, the HUVEC were collected for test of the apoptosis rate, MMP, and GRP78 protein (reflecting ERS). Results. In the model control group, the apoptosis rate and GRP 78 protein expression of HUVEC significantly increased (P < 0.05), while MMP significantly decreased (P < 0.05) compared with the blank control group. After GXK treatment of medium and high doses, the apoptosis rate and the GRP 78 protein expression significantly (P < 0.05) decreased, while MMP significantly increased (P < 0.05) in a time-dependent manner compared with the model control group. Conclusion. GXK can antagonize the injury of HUVEC caused by HCY and the antagonism effect increases with the concentration and treatment duration of GXK, with the possible mechanism of GXK antagonism being through inhibiting ERS caused by HCY. © 2014 Hao Wang et al.

Liu Y.,Hebei Medical University | Liu Y.,Key Orthopaedic Biomechanics Laboratory of Hebei Province | Peng M.,252 Hospital of Peoples Liberation Army | Lin L.,Second Hospital of Cangzhou City | And 3 more authors.
Osteoporosis International | Year: 2015

Summary: This study retrospectively reviewed 327 nonagenarians who underwent hip fracture surgery at six hospitals. Functional status, postoperative complications, and 1-year mortality were evaluated, and relationships between these factors and American Society of Anesthesiologists (ASA) grade were analyzed. ASA grade was significantly associated with postoperative complications and 1-year mortality.Introduction: Few previous studies have reported outcomes after hip fracture in nonagenarians, and these studies did not report significant associations between ASA grade and mortality. However, most of these studies included only a small number of patients from a single hospital. This study aimed to evaluate the relationships between ASA grade and functional status, postoperative complications, and mortality rate in nonagenarians undergoing hip fracture surgery.Methods: This study included 327 nonagenarians who underwent hip fracture surgery between January 2000 and December 2012. Patients with open fractures, subtrochanteric fractures, polytrauma, and pathological fractures were excluded. The medical records and X-rays were retrospectively reviewed. The relationships between ASA grade and functional status, postoperative complications, and 1-year mortality were analyzed.Results: There were significant associations between the ASA grade and the rates of postoperative complications and 1-year mortality (both p < 0.05). All pairwise comparisons showed significant differences in postoperative complication rates between ASA grades (all p < 0.05). All pairwise comparisons, except for grades I vs. II and grades II vs. III, also showed significant differences in mortality rates between ASA grades (all p < 0.05). There were significant associations between the preoperative ability to manage activities of daily living and the rates of postoperative complications and 1-year mortality (both p < 0.05).Conclusions: ASA grade was significantly associated with the rates of postoperative complications and 1-year mortality in nonagenarians undergoing hip fracture surgery. The preoperative functional status was also significantly associated with these outcomes. © 2014, International Osteoporosis Foundation and National Osteoporosis Foundation.

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