First Hospital of Qinhuangdao City
First Hospital of Qinhuangdao City
Gao S.,Liaoning Medical University |
Zhang Z.-M.,Jinzhou Municipal Second Hospital |
Shen Z.-L.,Jinzhou Municipal Second Hospital |
Gao K.,Jining No 1 Peoples Hospital |
And 5 more authors.
Neural Regeneration Research | Year: 2016
Atorvastatin, a lipid-lowering medication, provides neuroprotective effects, although the precise mechanisms of action remain unclear. Our previous studies confirmed activated autophagy following spinal cord injury, which was conducive to recovery of neurological functions. We hypothesized that atorvastatin could also activate autophagy after spinal cord injury, and subsequently improve recovery of neurological functions. A rat model of spinal cord injury was established based on the Allen method. Atorvastatin (5 mg/kg) was intraperitoneally injected at 1 and 2 days after spinal cord injury. At 7 days post-injury, western blot assay, reverse transcription-polymerase chain reaction, and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining results showed increased Beclin-1 and light chain 3B gene and protein expressions in the spinal cord injury + atorvastatin group. Additionally, caspase-9 and caspase-3 expression was decreased, and the number of TUNEL-positive cells was reduced. Compared with the spinal cord injury + saline group, Basso, Beattie, and Bresnahan locomotor rating scale scores significantly increased in the spinal cord injury + atorvastatin group at 14-42 days post-injury. These findings suggest that atorvastatin activated autophagy after spinal cord injury, inhibited apoptosis, and promoted recovery of neurological function. © 2016, Editorial Board of Neural Regeneration Research. All rights reserved.
Li J.,Yanshan University |
Shi M.,Yanshan University |
Ma B.,Hebei Normal University of Science and Technology |
Zheng Y.,First Hospital of Qinhuangdao City |
And 2 more authors.
Food and Function | Year: 2017
Potassium bromate (KBrO3) is widely used as a food additive and is a major by-product of water disinfection. The aim of this study was to investigate the protective effects of fraction 4a of polysaccharides isolated from Lycium barbarum (LBP-4a) against renal damage induced by KBrO3 in rats and to determine the relevant mechanisms behind these effects. Male Wistar rats were divided into a normal control group, a KBrO3 control group, and LBP-4a(L) and LBP-4a(H) groups. With the exception of the normal control group, the rats in the other three groups were administered KBrO3 by intraperitoneal injection to induce renal damage. Before the induction of renal damage, rats in the LBP-4a(L) and LBP-4a(H) groups were pretreated with 50 mg per kg b.w. or 100 mg per kg b.w. LBP-4a, respectively, by intragastric administration. Clinical biochemical parameters and markers of oxidative damage were also determined. Treatment by administration of LBP-4a prior to challenge with KBrO3 prevented increases in the levels of nitrite, creatinine, urea nitrogen and uric acid in serum and increased the activities of antioxidant enzymes in kidney tissues. A comet assay and FCS results showed that pretreatment with LBP-4a also alleviated DNA damage and decreases in mitochondrial membrane potentials in renal cells. Histological studies further supported the above results and showed extensive renal damage in animals treated with KBrO3 and greatly reduced tissue injury in groups pretreated with LBP-4a. In conclusion, LBP-4a exhibited protective effects against renal damage induced by KBrO3, and the mechanism was closely correlated with a reduction in levels of lipid peroxidation and an increase in the activities of antioxidant enzymes in kidney tissues, which alleviated DNA damage and increased mitochondrial membrane potentials in renal cells. These observations provide the background for the further development of LBP-4a as a protective agent for use in the treatment of renal damage. © 2017 The Royal Society of Chemistry.
Li R.,Hospital of Qinhuangdao Port Group Ltd. |
Tang X.,First Hospital of Qinhuangdao City |
Hao X.,Hospital of Qinhuangdao Port Group Ltd. |
Zhang Z.,Hospital of Qinhuangdao Port Group Ltd. |
And 3 more authors.
Chinese Journal of Andrology | Year: 2010
Objective: To study the effect of epigallocatcchin-3-gallate (EGCG) combined with bicalutamide on proliferation and apoptosis of human prostate cancer androgen-dependent cell lines LNCaP and its mechanism. Methods: LNCaP cells were treated respectively with different concentration of EGCG, bicalutamide and their combination. The proliferation and apoptosis of the treated cells were measured by methylthiazolyl tetrazolium (MTT) assay and flow cytometry (FCM). The expression of androgen receptor (AR), prostate-specific antigen (PSA) and cleaved caspase-3 were deteted by Western blot. Results: The proliferation of LNCaP cells was significant inhibited and the apoptosis of LNCaP cells was significant induced under treatment of EGCG ( with a concentration of 25 μmol/L 50, 50 μmol/L, 100 μmol/L) and bicalutamide (with a concentration of 30 μ mol/L) alone, as well as their combination, compared with that of controls (P<0.01). The proliferation and apoptosis of the treated cells in the combined treatment group were significantly different from that of other alone use group (P<0.01), which indicated that there was a synergistic effect of the two agents in the proliferation and apoptosis of LNCaP. The expressions of AR and PSA were apparently downregulated, as well as the expression of cleaved caspase-3 was upregulated after treatment of EGCG(50 μmol/L), bicalutamide (30 μ mol/L) alone and in combination for 72 hours. Conclusion: The proliferation of LNCaP cells was significant inhibited and the apoptosis of LNCaP cells was significant induced under treatment of EGCG and bicalutamide alone, as well as their combination, and a synergistic effect of EGCG and bicalutamide was found in the proliferation and apoptosis of LNCaP. The apoptosis of LNCaP cells induced by EGCG and bicalutamide might be mediated by AR and caspase-3. The level of PSA is useful to monitor the state of patient's tumor burden.
Niu H.-T.,First Hospital of Qinhuangdao City |
Wang Z.-J.,First Hospital of Qinhuangdao City |
Zhai R.-Y.,Capital Medical University |
Wang J.-F.,Capital Medical University |
And 2 more authors.
Chinese Journal of Radiology (China) | Year: 2012
Objective: In-hospital mortality rate in patients undergoing percutanous transhepatic biliary drainage for malignant obstructive jaundice remained high. This study aimed to assess pre-, intra-and post-procedure risk factors which were independently associated with increased in-hospital mortality. Methods: One hundred and fifty-five consecutive patients with malignant obstructive jaundice received initial PTBD drainage. Twenty-five pre-procedure, 4 intra-procedure and 6 post-procedure factors potentially related with in-hospital mortality were assessed by univariate and multivariate analysis. Results: In-hospital mortality rate was 16.8% (26/155). Of 25 pre-procedure variables analysed, Child-Pugh classification C, creatinine (≥6.93 μmol/L) and quality of life (≤30) were found to be significant in univariate and multivariate analysis. Increased mortality was seen in this study with two or more risk factors, significantly different from patients who had none or one risk factor (P < 0.01). None of the intra-procedure factors were important in identifying patients at risk of death. Multivariate analysis indicated post-PTBD cholangitis and unsuccessful drainage as post-procedure risk factors that correlated with in-hospital death. Conclusions: Three pre-procedure and two post-procedure risk factors were identified associated with in-hospital mortality. Copyright © 2012 by the Chinese Medical Association.
Gu T.,First Hospital of Qinhuangdao City
Chinese Journal of Oncology | Year: 2011
Objective: To explore the clinical and physical factors that might give rise to radiation-induced esophagitis in three-dimensional conformal radiotherapy for non-small cell lung cancer. Methods: To collect the clinical and physical records and follow-up information of 106 NSCLC patients without undergoing surgery in our hospital, χ2 test, linear tendency test and analysis of variance were employed to analyze the relationship between occurrence of radiation-induced esophagitis and clinical and physical treatment. Logistic analysis was also used for multivariate analysis. Results: Among the 47 cases of radiation-induced esophagitis, 31 cases were of grade I , 11 of grade II, 5 of grade III, and with a total occurrence rate of 44.3% (47/106). Radiation-induced esophagitis was correlated with Karnofsky scores, radiation sensitization and tumor location (χ2 = 11.30, 8.45, 7.67, P < 0.05). Radiation-induced esophagitis was correlated with the length of irradiated esophagus and average dose of irradiated esophagus (.F = 20.82, 83.08, P < 0.001). With the increase of the irradiated volume percentage from V20, V30, V40 up to V50, the occurrence rate of radiation-induced esophagitis was also increased, almost with a linear trend (P < 0.05). Application of all the above factors to logistic model indicated that radiation sensitization, length of irradiated esophagus, average dose and V50 were all statistically significant foactors in the occurrence of radiation-induced esophagitis (OR =0.321, 2.850, 7.307 and 8.558, P <0.05). Conclusions: Radiation sensitization, length of irradiated esophagus, average dose of irradiated esophagus and V50 are independent factors in the ocurrence of radiation-induced esophagitis. V50 is of greater importance in the judgement of ocurrence of radiation-induced esophagitis.
Cao J.,First Hospital of Qinhuangdao City |
Wang H.,First Hospital of Qinhuangdao City |
Qian Z.,First Hospital of Qinhuangdao City |
Zhang H.,First Hospital of Qinhuangdao City |
And 2 more authors.
Journal of Leukemia and Lymphoma | Year: 2014
Objective To explore the clinical significance of plasma D-dimer level before and after chemotherapy in patients with malignant lymphoma. Methods 402 patients admitted to Tianjin Medical University Cancer Institute and Hospital and pathologically diagnosed with malignant lymphoma were retrospectively analyzed to investigate the relationship between patients' plasma D-dimer level and their clinic pathology. Meanwhile, the association between patients ' plasma D-dimer level change after chemotherapy and therapeutic effect was also evaluated. Results The median plasma D-dimer levels in malignant lymphoma patients (734.51ng/ml) was distinctly higher than that in normal population (<500 ng/ml). The plasma D-dimer level had obvious correlation with age, pathological type, level of LDH, clinical stage, B symptom and IPI score. The level of plasma D-dimer in positive response group significantly decreased from 949.40 ng/ml to 499.88 ng/ml after chemotherapy (P < 0.05), whereas that in the negtive response group significantly increased from 611.09 ng/ml to 899.76 ng/ml (P < 0.05). Conclusion The level of plasma D-dimer may provide the basis for evaluating the chemotherapeutic effect in patients with malignant lymphoma. © 2014, Editorial Board of Journal of Leukemia and Lymphoma, All rights reserved.
Jiang L.,First Hospital of Qinhuangdao City |
Jin H.,First Hospital of Qinhuangdao City |
Wang Z.,First Hospital of Qinhuangdao City |
Meng J.,First Hospital of Qinhuangdao City
Chinese Journal of Clinical Oncology | Year: 2010
Objective: To investigate the effect of G-CSF application after chemotherapy on gynecological tumors. Methods: Sixty-three patients with confirmed gynecological tumors were enrolled in this study. The patients were randomized into 2 groups: the treatment group (n=32) received a preventive dose of 150μg/d, IH G-CSF before II degree arrest of bone marrow and a therapeutic dose of 150μg/d, IH G-CSF after II degree arrest of bone marrow and the control group received therapeutic doses of 300μg/d, IH G-CSF after III degree arrest of bone marrow. Results: The treatment group had a lower rate of serious bone marrow arrest and a shorter time for WBC recovery than the control group. These differences were significant (P<0.05). Conclusion: Applying G-CSF promptly after degree bone marrow arrest plays a significant role in preventing serious bone marrow arrest.
Zhang Y.-J.,First Hospital of Qinhuangdao City |
Cao L.-Y.,First Hospital of Qinhuangdao City |
Fu Z.-Z.,First Hospital of Qinhuangdao City |
Wang Y.-J.,First Hospital of Qinhuangdao City |
And 2 more authors.
Journal of Cancer Research and Therapeutics | Year: 2015
Objective: To investigate the value of lysophosphatidic acid (LPA) in the diagnosis of ovarian cancer. Materials and Methods: We first performed a hospital-based, case-control study involving 123 ovarian cancer patients and 101 benign ovarian tumor patients, and then conducted a meta-analysis with 19 case-control studies to assess the correlation between ovarian cancer and plasma LPA levels. Results: The case-control study results demonstrated that ovarian cancer patients have increased LPA and cancer antigen (CA)-125 levels compared to patients with benign ovarian tumor (LPA: Ovarian cancer vs benign ovarian tumor: 5.28 ± 1.52 vs 1.82 ± 0.77 μmol/L; CA-125: Ovarian cancer vs benign ovarian tumor: 87.17 ± 45.81 vs. 14.03 ± 10.14 U/mL), which showed statistically significant differences (both P < 0.05). LPA with advanced sensitivity, specificity, positive predictive value, negative predictive value, and accuracy rate of diagnosis excelled CA-125 in the diagnosis of ovarian cancer (both P < 0.05). The areas under the receiver operating characteristic (ROC) curve in the diagnosis of ovarian cancer (LPA: 0.983; CA-125: 0.910) were statistically significant compared with the reference (both P < 0.001) and the difference of the areas of ROC curve between LPA and CA-125 in the diagnosis of ovarian cancer showed statistically significant difference (P < 0.05). The meta-analysis results suggested that plasma LPA levels were higher in ovarian cancer tissues than in benign tissues (standardized mean difference (SMD) =2.36, 95% confidence interval (CI): 1.61-3.11, P < 0.001) and normal tissues (SMD = 2.32, 95% CI: 1.77-2.87, P < 0.001). Conclusion: LPA shows greater value in the diagnosis of ovarian cancer compared to CA-125 and may be employed as a biological index to diagnose ovarian cancer.