Zhang Y.,Peking University |
Wen Z.,Tongji University |
Guan L.,Peking University |
Jiang P.,Tongji University |
And 4 more authors.
Anesthesiology | Year: 2015
Background: Systemic inflammation is a key feature in acid aspiration-induced acute respiratory distress syndrome (ARDS), but the factors that trigger inflammation are unclear. The authors hypothesize that extracellular histones, a newly identified inflammatory mediator, play important roles in the pathogenesis of ARDS. Methods: The authors used a hydrochloric acid aspiration-induced ARDS model to investigate whether extracellular histones are pathogenic and whether targeting histones are protective. Exogenous histones and antihistone antibody were administered to mice. Heparin can bind to histones, so the authors studied whether heparin could protect from ARDS using cell and mouse models. Furthermore, the authors analyzed whether extracellular histones are clinically involved in ARDS patients caused by gastric aspiration. Results: Extracellular histones in bronchoalveolar lavage fluid of acid-treated mice were significantly higher (1.832 ± 0.698) at 3 h after injury than in sham-treated group (0.63 ± 0.153; P = 0.0252, n = 5 per group). Elevated histones may originate from damaged lung cells and neutrophil infiltration. Exogenous histones aggravated lung injury, whereas antihistone antibody markedly attenuated the intensity of ARDS. Notably, heparin provided a similar protective effect against ARDS. Analysis of plasma from ARDS patients (n = 21) showed elevated histones were significantly correlated with the degree of ARDS and were higher in nonsurvivors (2.723 ± 0.2933, n = 7) than in survivors (1.725 ± 0.1787, P = 0.006, n = 14). Conclusion: Extracellular histones may play a contributory role toward ARDS by promoting tissue damage and systemic inflammation and may become a novel marker reflecting disease activity. Targeting histones by neutralizing antibody or heparin shows potent protective effects, suggesting a potentially therapeutic strategy. Copyright © 2014, the American Society of Anesthesiologists, Inc.
Sun G.G.,Tangshan Peoples Hospital |
Lu Y.F.,Tangshan Workers Hospital |
Fu Z.Z.,First Hospital of Qinhuangdao |
Cheng Y.J.,Hebei Medical University |
Hu W.N.,Tangshan Peoples Hospital
Tumor Biology | Year: 2014
This study aimed to analyze the expression, clinical significance of epithelial membrane protein-1 (EMP1) in nasopharyngeal carcinoma, and the biological effect in its cell line by EMP1 overexpression. Immunohistochemistry and Western blot were used to analyze the EMP1 protein expression in 75 cases of nasopharyngeal cancer and 31 cases of normal tissues to study the relationship between EMP1 expression and clinical factors. Recombinant lentiviral vector was constructed to overexpress EMP1 and then infect nasopharyngeal cancer CNE2 cell line. Quantitative real-time RT-PCR and Western blot were used to detect the mRNA level and protein of EMP1. MTT assay, cell apoptosis, migration, and invasion assays were also conducted to determine the influence of the upregulated expression of EMP1 that might be found on CNE2 cells' biological effect. Immunohistochemistry and Western blot: The level of EMP1 protein expression was found to be significantly lower in nasopharyngeal cancer tissue than in the normal tissues (P < 0.05). Decreased expression of EMP1 was significantly correlated with T stages, lymph node metastasis, clinic stage, and histological grade of patients with nasopharyngeal cancer (P < 0.05). Meanwhile, the loss of EMP1 expression correlated significantly with poor overall survival time by Kaplan-Meier analysis (P < 0.05). The result of biological function has shown that CNE2 cell-transfected EMP1 had a lower survival fraction, higher cell apoptosis, significant decrease in migration and invasion, higher caspase-9, and lower vascular endothelial growth factor C protein expression compared with CNE2 cell-untransfected EMP1 (P < 0.05). EMP1 expression decreased in nasopharyngeal cancer and correlated significantly T stages, lymph node metastasis, clinic stage, histological grade, and poor overall survival, suggesting that EMP1 may play important roles as a negative regulator to nasopharyngeal cancer cell. © 2013 International Society of Oncology and BioMarkers (ISOBM).
Heiden K.B.,Rush University Medical Center |
Williamson A.J.,Rush University Medical Center |
Doscas M.E.,Rush University Medical Center |
Ye J.,Rush University Medical Center |
And 6 more authors.
Journal of Clinical Endocrinology and Metabolism | Year: 2014
Context: Cancer stem cells (CSCs) have been recently identified in thyroid neoplasm. Anaplastic thyroid cancer (ATC) contains a higher percentage of CSCs than well-differentiated thyroid cancer. The signaling pathways and the transcription factors that regulate thyroid CSC self-renewal remain poorly understood.Objective: The objective of this study is to use two ATC cell lines (KAT-18 and SW1736) as a model to study the role of the sonic hedgehog (Shh) pathway in maintaining thyroid CSC self-renewal and to understand its underlying molecular mechanisms.Design: The expression and activity of aldehyde dehydrogenase (ALDH), a marker for thyroid CSCs, was analyzed by Western blot and ALDEFLUOR assay, respectively. The effect of three Shh pathway inhibitors (cyclopamine, HhAntag, GANT61), Shh, Gli1, Snail knockdown, and Gli1 overexpression on thyroid CSC self-renewal was analyzed by ALDEFLUOR assay and thyrosphere formation. The sensitivity of transfected KAT-18 cells to radiation was evaluated by a colony survival assay.Results: Western blot analysis revealed that ALDH protein levels in five thyroid cancer cell lines (WRO82, a follicular thyroid cancer cell line; BCPAP and TPC1, two papillary thyroid cancer cell lines; KAT-18andSW1736, twoATCcell lines) correlated with the percentage of the ALDHHigh cells as well as Gli1 and Snail expression. The Shh pathway inhibitors, Shh and Gli1 knockdown, in KAT-18 cells decreased thyroid CSC self-renewal and increased radiation sensitivity. In contrast, Gli1 overexpression led to increased thyrosphere formation, an increased percentage of ALDHHigh cells, and increased radiation resistance in KAT-18 cells. Inhibition of the Shh pathway by three specific inhibitors led to decreased Snail expression and a decreased number of ALDHHigh cells in KAT-18 and SW1736. Snail gene knockdown decreased the number of ALDHHigh cells in KAT-18 and SW1736 cells.Conclusions: The Shh pathway promotes the CSC self-renewal inATCcell lines by Gli1-induced Snail expression. Copyright © 2014 by the Endocrine Society.
Chen H.,First Hospital of Qinhuangdao |
Miao J.,Fourth Peoples Hospital of Taizhou |
Li H.,First Hospital of Qinhuangdao |
Wang C.,First Hospital of Qinhuangdao |
And 5 more authors.
Journal of Surgical Research | Year: 2014
Background: p21-activated protein kinase (PAK) 6 is a serine-threonine kinase belonging to the PAK family. Previous studies have indicated that abnormal expressions of PAK1, PAK2, and PAK5 played critical roles in hepatocellular carcinoma (HCC). Recent studies suggested that deregulation of PAK6 expression played an important role in oncogenesis. To explore the potential roles of PAK6 in HCC, expression of PAK6 was detected in human HCC specimens. Methods: Immunohistochemistry and Western blot analysis were performed for PAK6 in 121 HCC samples. The data were correlated with clinicopathologic features. The univariate and multivariate survival analyses were also performed to determine their clinical prognostic significance. Results: PAK6 was overexpressed in HCC as compared with the adjacent noncancerous liver tissues. High expression of PAK6 was associated with Edmondson-Steiner grade (P = 0.006) and number of tumor nodules (P < 0.001), and PAK6 was positively correlated with proliferation marker Ki-67 (P < 0.01). Univariate analysis suggested that PAK6 expression was associated with poor prognosis (P < 0.001). Multivariate analysis indicated that PAK6 and Ki-67 protein expressions were independent prognostic markers for HCC (P = 0.0245 and 0.0331, respectively). Conclusions: Our results suggest that PAK6 overexpression is involved in the pathogenesis of HCC; it may be an independent poor prognostic factor for HCC. © 2014 Elsevier Inc. All rights reserved.
Lu Q.,First Hospital of Qinhuangdao |
Ma C.,First Hospital of Qinhuangdao |
Yin F.,First Hospital of Qinhuangdao |
Wang R.,First Hospital of Qinhuangdao |
And 2 more authors.
European Journal of Pediatrics | Year: 2013
The present study evaluated the feasibility and accuracy of the blood pressure-to-height ratio (BPHR) and proposed the optimal thresholds of BPHR for identifying hypertension in Han children aged 7-12 years. In 2011, anthropometric measurements were assessed in a cross-sectional population-based study of 1,352 Han children aged 7-12 years. Hypertension was defined according to the 2004 National High Blood Pressure Education Program Working Group definition (as gold standard). The following equations for BPHR were used: systolic blood pressure-to-height ratio (SBPHR) = SBP (mmHg)/height (cm) and diastolic blood pressure-to-height ratio (DBPHR) = DBP (mmHg)/height (cm). Receiver operating characteristic curve analyses were performed to assess the accuracy of SBPHR and DBPHR as diagnostic tests for elevated SBP and DBP, respectively. After the cutoff points were determined, hypertension was defined by SBPHR/DBPHR (new standard), and the sensitivity and specificity were calculated. The accuracy of SBPHR and DBPHR (assessed by area under the curve) for identifying elevated SBP and DBP was over 0.85 (0.946-1.000). SBPHR cutoff values for elevated SBP were calculated to be 0.76-0.88 mmHg/cm in boys and 0.78-0.90 mmHg/cm in girls. DBPHR cutoff values for elevated DBP were calculated to be 0.51-0.60 mmHg/cm in boys and 0.51-0.58 mmHg/cm in girls. When hypertension was defined by BPHR, the sensitivities were 100 % in boys and 95.0 % in girls. The specificity was 94.3 % in boys and 96.8 % in girls. BPHR is a simple, inexpensive, and accurate index for screening hypertension in Han children. © 2012 Springer-Verlag.
Zhao M.,First Hospital of Qinhuangdao |
Zhang H.,First Hospital of Qinhuangdao |
Zhou Y.,First Hospital of Qinhuangdao |
Huang J.,First Hospital of Qinhuangdao |
Liu R.,First Hospital of Qinhuangdao
Cancer Research and Clinic | Year: 2016
Objective: To investigate the expression and clinical significance of serum response factor (SRF) and vascular endothelial growth factor receptor (VEGFR2) in gastric carcinoma. Methods: SABC immunohistochemical method was used to determine the expressions of SRF and VEGFR2 in 50 cases of gastric carcinoma, 50 cases of surgery incisal edges and 29 cases of lymph node metastasis focus. Results: The detection positive rates of SRF and VEGFR2 in gastric carcinoma were 52.00 % (26/50) and 60.00 % (30/50), respectively, which were significantly higher than those in the normal gastric mucosa [16.00 % (8/50) and 10.00 % (5/50), respectively] (P < 0.05), with no statistical difference with metastiatic lymph node [65.52 % (19/29) and 72.41 % (21/29), respectively]. In the gastric carcinoma group, the expression of SRF was relevant with depth of invasion and lymph node metastasis (P < 0.05). The expression of VEGFR2 was not related to age, gender and tumour size (P > 0.05), but closely correlated to differentiation degree, invasion depth and lymph node metastasis (P < 0.05). The expressions of SRF and VEGFR2 in the gastric carcinoma were positively correlated (r = 0.594, P < 0.05). Conclusion: Overexpressions of SRF and VEGFR2 in gastric carcinoma can be regarded as the poorly prognostic markers and play an important role in invasion and metastasis of gastric carcinoma. © Copyright 2016 by the Chinese Medical Association.
Wang J.,Yanshan University |
Wang J.,First Hospital of Qinhuangdao
International Journal of Optics | Year: 2016
In view of the present chaotic image encryption algorithm based on scrambling (diffusion is vulnerable to choosing plaintext (ciphertext) attack in the process of pixel position scrambling), we put forward a image encryption algorithm based on genetic super chaotic system. The algorithm, by introducing clear feedback to the process of scrambling, makes the scrambling effect related to the initial chaos sequence and the clear text itself; it has realized the image features and the organic fusion of encryption algorithm. By introduction in the process of diffusion to encrypt plaintext feedback mechanism, it improves sensitivity of plaintext, algorithm selection plaintext, and ciphertext attack resistance. At the same time, it also makes full use of the characteristics of image information. Finally, experimental simulation and theoretical analysis show that our proposed algorithm can not only effectively resist plaintext (ciphertext) attack, statistical attack, and information entropy attack but also effectively improve the efficiency of image encryption, which is a relatively secure and effective way of image communication. © 2016 Jian Wang.
Lu Q.,First Hospital of Qinhuangdao |
Ma C.M.,First Hospital of Qinhuangdao |
Yin F.Z.,First Hospital of Qinhuangdao |
Liu B.W.,First Hospital of Qinhuangdao |
And 2 more authors.
Journal of Human Hypertension | Year: 2011
Diagnosis of hypertension in adolescents is complicated because blood pressure values vary with age, gender and height. How can we simplify the diagnostic criteria for hypertension in adolescents? In 2006, anthropometric measurements were assessed in a cross-sectional population-based study of 3136 Han adolescents aged 13-17 years. Hypertension was defined according to the 2004 National High Blood Pressure Education Program Working Group definition. The following equations for blood pressure-to-height ratio (BPHR) were used: systolic BPHR (SBPHR)SBP (mm Hg)/height (cm) and diastolic BPHR (DBPHR)DBP (mm Hg)/height (cm). Receiver-operating characteristic curve analyses were performed to assess the accuracy of SBPHR and DBPHR as diagnostic tests for elevated systolic blood pressure (SBP) and diastolic blood pressure (DBP), respectively. After the cutoff points were determined, hypertension was defined by SBPHR/DBPHR, and the sensitivity and specificity were calculated. The accuracy of SBPHR and DBPHR (assessed by area under the curve) for identifying elevated SBP and DBP was ≥0.85 (0.989-1.000). The optimal thresholds of SBPHR/DBPHR for defining hypertension (stages 1 and 2) were 0.75/0.48 for boys and 0.78/0.51 for girls, and for defining hypertension (stage 2) were 0.81/0.57 for boys and 0.84/0.63 for girls. In identifying hypertension, the sensitivity and specificity were both <90% (91.0-99.1%). In identifying stage 2 hypertension, when the sensitivity was 100%, the specificity was 98.6% for boys and 99.1% for girls. BPHR is a simple, accurate and non-age-dependent index for screening hypertension in Han adolescents, especially for stage 2 hypertension. © 2011 Macmillan Publishers Limited All rights reserved.
Gao L.,First Hospital of Qinhuangdao
Cancer Research and Clinic | Year: 2015
Objective: To discuss the correlation between SIRT3 protein and clinicopathological parameters of gastric carcinoma. Methods: Immunohistochemistry and Western blot were used to detect the expression of SIRT3 in the gastric carcinoma and normal gastric tissue.The correlation between the expression of SIRT3 and clinicopathological parameters was analyzed. Results: The immunohistochemistry showed that the positive expression rate of SIRT3 protein in gastric carcinoma tissue (53.8 %, 43/80) was obviously lower than that in normal gastric tissue (86.0 %, 43/50), and the expression of SIRT3 protein showed close relationship with invasion depth, lymph node metastasis and TNM stage (P < 0.05), rather than the age, gender, tumor size, or differentiation status (P > 0.05). The Western blot showed that the expression rate of SIRT3 protein (SIRT3/β-actin) in gastric carcinoma tissue (0.655±0.317) was lower than that in normal gastric tissue (0.803±0.329) (P < 0.05). Conclusion The expression of SIRT3 protein is lower in gastric cancer than that in normal gastric tissue,and relates to invasion depth, lymph node metastasis and TNM stage. SIRT3 may inhibit the occurrence and development of gastric cancer.
Fu Z.,First Hospital of Qinhuangdao |
Sun G.,First Hospital of Qinhuangdao |
Sun G.,Tangshan Peoples Hospital |
Gu T.,First Hospital of Qinhuangdao
Tumor Biology | Year: 2014
This study aimed to analyze the expression, clinical significance of proto-oncogene in non small cell lung cancer (NSCLC), and the biological effect in its cell line by siRNA targeting wild-type p53-induced phosphatase 1 (Wip1). Immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) were, respectively, used to analyze Wip1 protein expression in 75 cases of NSCLC and normal tissues to study the relationship between Wip1 expression and clinical parameters. Wip1 siRNA was transiently transfected into papillary NSCLC H1299 cell by liposome-mediated method and was detected by RT-PCR and Western blot. MTT assay, cell apoptosis, and cell cycle were also conducted as to the influence of the downregulated expression of Wip1 that might be found on H1299 cells biological effect. The positive rates of Wip1 protein was 69.3 % in NSCLC tissues but 16.0 % expressed in normal tissues (P < 0.05). The relative content of Wip1 mRNA was 0.785 ± 0.062 and 0.147 ± 0.020 in NSCLC tissues and normal tissues, respectively, with significant differences between the two types (P < 0.05). There were no significant differences between Wip1 expression and sex, age, tumor size, and pathological types (P > 0.05). However, there were significant differences between Wip1 expression and lymph node metastasis, clinical stages, and tumor differentiation (P < 0.05). Individuals with positive and negative levels of Wip1 expression showed were statistically significant differences in the 5-year overall survival rate (P < 0.05). RT-PCR and Western blot showed that H1299 cell transfected Wip1 siRNA had a lower relative expressive content than normal cell (P < 0.05). MTT assay, cell apoptosis, and cell cycles demonstrated that H1299 cell transfected Wip1 siRNA had a lower survival fraction, higher cell apoptosis, more percentage of the G0/G1 phases, and lower cells in the S phases (P < 0.05). Wip1 protein and mRNA were increased in NSCLC, specifically in lymph node metastasis, clinical stages, and tumor differentiation. Wip1 may be involved in the biological processes of NSCLC cell proliferation, cell apoptosis, and cell cycle. © 2013 International Society of Oncology and BioMarkers (ISOBM).