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Xu X.,First Hospital of Putian City | Lin J.,First Hospital of Putian City | Zheng M.,First Hospital of Putian City | Huang C.,First Hospital of Putian City | And 2 more authors.
Cancer Research and Clinic | Year: 2016

Objective To explore the relationship of the methylation levels of tissue inhibitor of metalloproteinases 3 (TIMP-3) gene promoter CpG islands with the invasiveness and prognosis in cardia carcinoma. Methods The tumors tissues were collected from 65 patients with cardia carcinoma. The methylation levels of the promoter of TIMP-3 gene were detected by methylation-specific PCR (MSP), the mRNA expression levels of TIMP-3 gene were detected by RT-PCR. The relationship of TIMP-3 gene ectopic methylation with invasiveness and prognosis of the cardia carcinoma patients was analyzed. Results The positive expression rates of TIMP-3 mRNA in gastric cancer and normal gastric tissues were 53.8 % (35/65) and 96.9 % (63/65), respectively. The difference was not statistically significant (Fisher exact test, P = 0.912). The positive rate of TIMP-3 mRNA was negatively correlated with the depth of tumor invasion and lymph node metastasis [mucosa and muscular vs. serosa and mucosa outside of the serosa and muscula: 83.3 % (10/12) vs. 45.3 % (24/53); with lymph node metastasis vs. without lymph node metastasis: 73.9 % (17/23) vs. 40.5 % (17/42)] (both P < 0.05). There was a negative correlation between TIMP-3 gene promoter methylation and TIMP-3 mRNA expression (r=-0.276, P = 0.026). The size of tumor and TIMP-3 gene promoter methylation were both the independent influencial factors of prognosis in cardia carcinoma (both P < 0.05). Conclusion The methylation of promoter region in CpG islands plays an important role in the invasion and metastasis of cardia carcinoma, and it can be used as an independent predictor of biological behavior and prognosis.


Li W.,Sun Yat Sen University | Li W.,First Hospital of Putian City | Zhang P.,Sun Yat Sen University | Chen Q.,Sun Yat Sen University | And 3 more authors.
Journal of Craniofacial Surgery | Year: 2015

The purpose of this study was to report an unusual case of severe microphthalmos, together with an orbital cyst and huge ocular surface dermolipoma. Methods: This is a clinical report relating clinical features as well as imaging and histopathologic findings, along with surgical management of the patient. Results: A 5-month-old Chinese male infant was referred, with 2 large masses in the left eye that were present since birth. Ocular examination results revealed a complete absence of any eye structures in the left orbit. In its place were 2 large masses between the left upper and lower palpebral fissure. One was a 3 × 3 × 2.5-cm spherical red tumor with a smooth surface. The other was a large solid spherical tumor, 4 × 4 × 5 cm, covered with normal skin located in the temporal region and attached to the red mass by a pedicle. Orbital magnetic resonance imaging examination findings confirmed that no eye structures were present in the left orbit. However, a cystic lesion was found in the left orbit, with a low signal on T1-weighted imaging and high signal on T2-weighted imaging, and another huge spherical heterogeneous mass was located "outside" the left orbit. Anterior orbitotomy by conjunctival incision was performed under general anesthesia. A spherical cystic mass of 1.5 × 1.5 × 1.6 cm, a small eye of 0.7 × 0.7 × 0.6 cm, and a huge dermolipoma were removed completely. Pathologic examination results confirmed the diagnosis of severe microphthalmos, together with orbital dermoid cyst and dermolipoma. Conclusions: This rare case demonstrates that severe microphthalmos with a cyst may be completely covered by conjunctiva and associated with an unusually huge dermolipoma. © 2015 Mutaz B. Habal, MD.


Zhang W.,First Hospital of Putian City | Huang Z.,First Hospital of Putian City | Yan J.,Sun Yat Sen University
Journal of Craniofacial Surgery | Year: 2013

BACKGROUND: The study aims to report a rare case of traumatic lysis of inferior rectus muscle after a traffic accident. METHODS: This is a case report wherein the clinical features, computed tomographic findings, and surgical treatment were presented. RESULTS: A 42-year-old woman complained of double vision and upward deviation of the left eye for 13 months after being involved in a traffic accident. The alternate prism and cover test both at distance and near testing showed an exotropia of 18 prism diopters (PD) and hypertropia of 50 PD of the left eye. The left eye had complete restriction of downward movement, and the forced duction test was negative. Computed tomography disclosed that the middle portion of the left inferior rectus muscle was absent. Diagnosis was traumatic lysis of left inferior rectus muscle. The deviation of the left eye was corrected by left superior rectus muscle recession and partial transposition of the medial and lateral rectus muscles. Six months after surgery, no deviation at the primary position both at distance and near testing was present; however, the left eye remained at a -3 restriction of downward movement. CONCLUSIONS: Paralysis of the inferior rectus muscle may have resulted from the traumatic lysis, a rare clinical feature that can be confirmed by computed tomography. Copyright © 2013 Mutaz B. Habal, MD.


Chen G.-X.,Southern Medical University | Chen G.-X.,First Hospital of Putian City | Zheng S.,Southern Medical University | Qin S.,Peoples Hospital of Zhuhai | And 7 more authors.
PLoS ONE | Year: 2014

Background: Alendronate (ALE) is a conventional drug used to treat osteoporosis. Low-magnitude whole-body vibration (WBV) exercise has been developed as a potential treatment for osteoporosis. The aim of this study was to investigate whether low-magnitude WBV could enhance the protective effect of ALE on bone properties in ovariectomized rats. Methods: A total of 128 Sprague-Dawley rats were randomly divided into five groups (SHAM, OVX+VEH, OVX+WBV, OVX + ALE, OVX+WBV+ALE). The level of WBV applied was 0.3 g at 45-55 Hz for 20 min/day, 5 day/week and for 3 months. ALE was administered in dose of 1 mg/Kg once a week. Every four weeks eight rats from each group were sacrificed and their blood and both tibiae were harvested. The expression of osteocalcin and CTX in serum was measured by enzyme-linked immunosorbent assay (ELISA) and the tibiae were subjected to metaphyseal three-point bending and μCT analysis. Results: Osteocalcin rose after ovariectomy and was not appreciably changed by either alendronate or WBV alone or in combination. Alendronate treatment significantly prevented an increase in CTX. WBV alone treatment did not alter this effect. Compared with the OVX+WBV group, nearly all tested indices such as the BV/TV, TV apparent, Tb.N, Tb.Th, and Conn.D were higher in the OVX+ALE group at week 12.Compared with the OVX+WBV group, certain tested indices such as BV/TV, TV apparent, Tb.N, and Con.D, were higher in the OVX+WBV+ALE group at week 12. At week 12, tibiae treated with WBV+ALE exhibited a significantly higher Fmax compared to the OVX+VEH group, and a significant difference was also found in energy absorption between the OVX+WBV+ALE and OVX+VEH groups. Conclusions: Compared with the WBV, ALE was more effective at preventing bone loss and improved the trabecular architecture. However, WBV enhanced the effect of alendronate in ovariectomized rats by inducing further improvements in trabecular architecture. © 2014 Chen et al.


Xu X.,First Hospital of Putian City | Lin J.,First Hospital of Putian City | Zheng M.,First Hospital of Putian City | Huang C.,First Hospital of Putian City | And 2 more authors.
Cancer Research and Clinic | Year: 2016

Objective: To explore the relations among the promoter methylation of tissue inhibitor of metalloproteinase-3 (TIMP-3) gene and its protein expression, and the clinicopathological features in the gastric cardia carcinoma. Methods: The tumor tissues and the adjacent normal mucosal tissues were collected from 65 patients with cardia carcinoma. The promoter methylation levels and the protein expression of TIMP-3 gene were detected by methylation-specific PCR (MSP) and immunohistochemistry respectively. Results: The TIMP-3 methylation rates was 78.5 % (51/65) in the tumor tissues and 13.8 % (9/65) in the incisai edge of normal tissues, the methylation rates of TIMP-3 had positive correlation with the size of tumor, invasion depth, lymphatic metastasis and the stage of tumor. The protein expression of TIMP-3 was 26.2 % (17/65) in the tumor tissues and 95.4 % (62/65) in the incisai edge of normal tissues (P = 0.016), the protein expression of TIMP-3 was negatively correlated with the size of tumor, invasion depth, lymphatic metastasis and the stage of tumor. Conclusion: The methylation of promoter region in CpG islands is a main mechanism of reduced and loss expression of TIMP-3 gene, which may play an important role in the invasion and metastasis of cardia carcinoma.


Peng Y.,Huazhong University of Science and Technology | Peng Y.,First Hospital of Putian City | Lu Y.,Tongji Hospital | Wei W.,Huazhong University of Science and Technology | And 5 more authors.
Journal of Stroke and Cerebrovascular Diseases | Year: 2015

Background Depression and anxiety are common after stroke. There is inconclusive evidence of the benefit of psychotherapy for poststroke depression and anxiety. Here, we used a brief intervention, Neuro-Linguistic Programming (NLP) brief therapy plus health education, to evaluate the changes in patients with ischemic stroke. Methods One hundred eighty patients were randomly allocated to receive 4 sessions of NLP plus health education (n = 90) or usual care (n = 90). A set of questionnaires was used preintervention and postintervention as well as at the 6-month follow-up. The primary outcomes were the prevalence of depression and anxiety, and the awareness of stroke knowledge. Results More patients in the intervention group achieved remission of depressive (odds ratio [OR], 2.81; 95% confidence interval [CI], 1.41-5.59) and anxious symptoms (OR, 2.19; 95% CI, 1.15-4.18) after intervention. At the 6-month follow-up, we found no differences between groups in both the prevalence of depression and anxiety. After intervention, the intervention group had better awareness rates on most of the stroke knowledge items (P <.05). It also had better quality of life and physical function both after intervention and at the follow-up (P <.05). Conclusions NLP plus health education could reduce depression and anxiety immediately after intervention, but not at the 6-month follow-up. The intervention could also improve the awareness of stroke knowledge and benefit patients on quality of life and physical function. © 2015 National Stroke Association.


Chen G.-X.,First Hospital of Putian City | Wang G.-R.,First Hospital of Putian City | Lin Z.-J.,First Hospital of Putian City | Li G.-S.,First Hospital of Putian City | And 4 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013

Background: Osteoclast as a terminal cell is difficult to obtain, and the shortage of mature osteoclast strains limits its application. Previous studies have shown that stromal cell culture or co-culture is commonly used to obtain the osteoclasts, and the receptor activator of nuclear factor κB ligand and macrophage colony-stimulating factor can be used in combination in order to obtain the mature osteoclasts. Objective: To observe the biological characteristics of mouse monocyte macrophage RAW264.7, and to analyze the feasibility of receptor activator of nuclear factor κB ligand-mediated differentiation of monocyte macrophage RAW264.7 into osteoclasts. Methods: The RAW264.7 cells were cultured, and then the RAW264.7 cells were induced with receptor activator of nuclear factor κB ligand for 7 days to observe the tartrate-resistant acid phosphatase staining results. The positive tartrate-resistant acid phosphatase staining and nucleus ≥ 3 were considered as the osteoclasts. Phalloidin staining was used to observe fibrous actin ring, and toluidine blue staining was used to observe the resorption pits on the surface of bovine bone slice. Results and Conclusion: RAW264.7 cells could be induced into tartrate-resistant acid phosphatase-positive multinuclear cells with receptor activator of nuclear factor κB ligand, and formed fibrous actin rings; round or ellipse bone resorption pits were found in bone slice surface by microscope. RAW264.7 cells were considered as a good preosteoclast model that used for research of osteoclast differentiation. Receptor activator of nuclear factor κB ligand can induce the differentiation and maturation of RAW264.7 cells, reduce the application of macrophage colony-stimulating factor and make the training system simpler and easier to operate, and the induced cells have the high purity suitable for biological and biochemical studies of osteoclasts.


Zeng Y.,North Zhongshan Road | Zeng Y.,Fujian Medical University | Hong M.,Traditional Chinese Medicine Hospital of Zhangzhou City | Zhang H.,Fujian Medical University | And 6 more authors.
Respirology | Year: 2010

Transbronchoscopic balloon detection and selective bronchial occlusion were applied in the management of 40 patients with intractable pneumothorax. An autologous blood clot was used as a bronchial sealant. The efficacy of the procedure, effect on oxygenation and radiographic changes in occluded areas of the lung were evaluated. Background and objective: The aim of this study was to evaluate the efficacy, complications and safety of the application of transbronchoscopic balloon detection (TBD) and selective bronchial occlusion (SBO) to intractable pneumothorax. Methods: Forty patients with pneumothorax, who had experienced more than 7 days of chest tube drainage without closure of the pleural fistula, underwent TBD and SBO. In 10 patients, oxygenation and pulse rates were recorded. A thoracic CT scan was performed 10 days after SBO. Results: The bronchi leading to the pleural fistula were located by TBD in 34 of 40 patients (85%). The air leakages ceased after the first occlusion in 30 patients, and five of these 30 patients underwent a second occlusion due to recurrence of pneumothorax 72 h after the first occlusion. In three of these patients, air leakages ceased after the second occlusion, while the remaining two patients underwent thoracoscopy. In total, 28 of 40 patients (70%) were cured using SBO. During TBD/SBO, the lowest SaO2 was 89.0 ± 2.8%, the mean SaO2 was 93.4 ± 2. 6% and the percentage of time during the procedure that SaO2 was <90% was 10.7 ± 17.5%. Ten days after SBO, thoracic CT scans were performed on 10 patients and no obstructive atelectasis was detected in any patient. Conclusions: These results indicate that TBD and SBO are safe and effective procedures for treating patients with intractable pneumothorax. © 2009 Asian Pacific Society of Respirology.

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