First Hospital of Ningbo City


First Hospital of Ningbo City

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Liu Z.,Gannan Medical University | Liu Z.,First Hospital of Ningbo City | Wang T.,Gannan Medical University | Wang T.,First Hospital of Ningbo City | And 2 more authors.
2011 International Conference on Electrical and Control Engineering, ICECE 2011 - Proceedings | Year: 2011

To investigate the antifungal activity of the compound preparation of traditional Chinese drug (KZY-2) on Candida albicans and Cryptococcus neoformans isolated from faecal specimen of pigeon in vitro. After KZY-2 effects on Candida albicans(ATCC32354, GFC1GFC10) and Cryptococcus neoformans(CCCMD2a, GFD1GFD10), the diameter of inhibited zone was measured adopting agar plate diffusion method and the MIC was measured Reference to the M27-A recommended by the United States National Laboratory Standards Committee (NCCLS). MFC were measured by colony counting method after agar plate culture and reference to the M27-A. The diameters of inhibited zone of KZY-2 against Candida albicans(ATCC32354) and Cryptococcus neoformans(CCCMD2a) were 28mm and 28mm, the MIC were 312.5g/m1 and 156.3g/m1, the MFC were 625g/m1 and 312.5g/m1. The diameter of inhibited zone of KZY-2 against Candida albicans(GFC1GFC10) which of seven were effective was between 12mm and 35 mm, the MIC was between 312.5g/m1 and 2500g/m1, the MFC were between 625g/m1 and 5000g/m1. The diameter of inhibited zone of KZY-2 against Cryptococcus neoformans(GFD1GFD10) which of nine were effective was between 18mm and 40mm, the MIC was between 156.3g/m1 and 625g/m1, the MFC were between 312.5g/m1 and 1250g/m1. These results show the diameters of inhibited zone and MIC were different when KZY-2 effects on different strain with the same drug concentration. Compared Candida albicans and Cryptococcus neoformans, the diameters of inhibited zone and MIC all had the significant difference, which the diameter of former was bigger, but the MIC of the latter was bigger. these results indicate The KZY-2 have good in vitro antifungal activity on Candida albicans and Cryptococcus neoformans whether which are the standard strains or isolated strains from faecal specimen of pigeon. © 2011 IEEE.

Chen X.-D.,First Hospital of Ningbo City | Tang S.-X.,First Hospital of Ningbo City | Zhang J.-H.,First Hospital of Ningbo City | Zhang L.-T.,First Hospital of Ningbo City | Wang Y.-W.,First Hospital of Ningbo City
Oncology Reports | Year: 2017

Laryngeal carcinoma is one of the most common malignant tumors in otorhinolaryngology. Moreover, experimental investigation showed that cancerous inhibitor of protein phosphatase 2A (CIP2A) expressed highly in various cancers. Therefore, we investigated whether CIP2A can regulate the proliferation, invasion and migration by RNA interference in Hep-2 cells and AMC-NH-8 cells and further affect the activation of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) pathway. Overexpression of CIP2A was evaluated in tumor tissue and laryngeal cancer cell lines (Hep-2 and AMC-NH-8 cells) by real-time quantitative polymerase chain reaction (RT-qPCR) and western blot assay. In a follow-up experiment, we confirmed that CIP2A siRNA effectively suppressed the cell proliferation at 48 and 72 h, and arrested cell cycle at G0/G1 in Hep-2 cells and AMC-NH-8 cells. The invasion and migration of cell in siRNA CIP2A group were markedly inhibited. Moreover, the experimental results showed that the expression levels of invasion-and migrationrelated genes, including E-cadherin, metastasis-associated gene 1 (MTA1) and matrix metalloproteinases-2/9 (MMP-2/9), were regulated by CIP2A siRNA. Phosphorylation levels of PI3K and AKT proteins were reduced by CIP2A siRNA. Importantly, it suggested signaling through PI3K/Akt as a critical mechanism by which CIP2A siRNA may suppress cell proliferation, invasion and migration in laryngeal carcinoma cells.

Zhu H.,First Hospital of Ningbo City | Chen Y.,First Hospital of Ningbo City | Huang C.,First Hospital of Ningbo City | Han Y.,First Hospital of Ningbo City | And 6 more authors.
Journal of Pharmaceutical Analysis | Year: 2015

Abstract A rapid and sensitive method based on ultrafast liquid chromatography-tandem mass spectrometry was developed and validated for simultaneous determination of Sudan I, Sudan II, Sudan III, and Sudan IV levels in rat whole blood. Cleanert C18 mixed-mode polymeric sorbent was used for effective solid-phase extraction cleanup. Separation was carried out on a reversed-phase C18 column (100 mm×2.1 mm, 1.8 μm) using 0.1% (v/v) formic acid in water/0.1% (v/v) formic acid in acetonitrile as the mobile phase in gradient elution. Quantification was performed by an electrospray ionization source in the positive multiple reaction monitoring mode using D5-Sudan I as the internal standard. Calibration curves showed good linearity between 0.2 and 20.0 μg/L, with correlation coefficients higher than 0.9990. The average recovery rates were between 93.05% and 114.98%. The intra- and inter-day relative standard deviations were within 6.2%. The lower limit of quantification was 0.2 μg/L. All the analytes were found to be stable in a series of stability studies. The proposed method was successfully applied to a pharmacokinetic study of four Sudan dyes after oral administration to rats. © 2015 The Authors.

Huang C.,First Hospital of Ningbo City | He J.,First Hospital of Ningbo City | Chen Y.,First Hospital of Ningbo City | Zhang Y.,First Hospital of Ningbo City | Chen C.,First Hospital of Ningbo City
Journal of Surgical Research | Year: 2014

Background Activated microglia play an important role in neuroinflammation, which contributes to the neuronal damage found in many neurodegenerative diseases. Penehyclidine hydrochloride (PHC) is an anesthetic used before surgical operations, but also exhibits anti-inflammatory effects on the respiratory and digestive system. In the present study, we investigated whether PHC produces similar anti-inflammatory effects in activated microglia in the central nervous system. Materials and methods Microglial cells were incubated with lipopolysaccharide (LPS) in the presence or absence of various concentrations of PHC, SB203580 (p38 mitogen-activated protein kinase [MAPK] inhibitor), and pyrrolidine dithiocarbamate (nuclear factor-kappa B [NF-κB] inhibitor). Markers of inflammation and oxidative stress were measured using enzyme-linked immunosorbent assay and quantitative real-time polymerase chain reaction. The effect of PHC on NF-κB activity was assessed with a NF-κB p50/p65 transcription factor assay kit. The involvement of p38 MAPK phosphorylation in the anti-inflammatory effects of PHC was evaluated with a specific enzyme-linked immunosorbent assay kit for phospho-p38. Results PHC significantly inhibited the release of nitric oxide, prostaglandin E2, interleukin 1β, and tumor necrosis factor α while upregulating the expression of inducible nitric oxide synthase messenger RNA in LPS-activated microglia. Moreover, PHC effectively inhibited the translocation of NF-κB from the cytoplasm to the nucleus and the phosphorylation of p38 MAPK. The activities of NF-κB and p38 MAPK in LPS-treated microglia were significantly lowered after pretreatment of PHC. Conclusions PHC inhibited the LPS-induced release of inflammatory mediators in microglia. These inhibitory effects of PHC may be mediated by blocking p38 MAPK and NF-κB pathways in microglia. These preclinical findings may offer a novel therapeutic option to confine microglial overactivation in neurodegenerative diseases. © 2014 Elsevier Inc. All rights reserved.

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