Jin Q.,Tongde Hospital of Zhejiang Province |
Huang F.-H.,Tongde Hospital of Zhejiang Province |
Zhou Y.,Tongde Hospital of Zhejiang Province |
Zhang Y.-Q.,First Hospital of Jiaxing |
Chai X.-J.,Tongde Hospital of Zhejiang Province
Acta Academiae Medicinae Sinicae | Year: 2012
Objective: To study the expressions of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the airway of bronchiectasis (BE) patients, and evaluate the effect of Pseudomonas aeruginosa (PAE) on the expression of TIMP-1 and MMP-9. Methods: In this case-control study, subjects were divided into BE group and control group, and the BE group was further divided into PAE group and other bacteria group based on the culture results of bronchoalveolar lavage fluid (BALF). BALF was obtained by bronchoscopy, the expressions of MMP-9 and TIMP-1 were determined by ELISA, then the ratio of TIMP-1/MMP-9 was calculated. Furthermore, the tissue of bronchic endomembrane was obtained by transbronchial biopsy and the expressions of MMP-9 and TIMP-1 were determined using immunohistochemical method. Results: The levels of MMP-9 in the BALF of PAE group and other bacteria group were significantly higher than that in control group (P = 0.0000 both), and the expressions of MMP-9 in bronchic endomembrane of PAE group and other bacteria group were also significantly higher (P = 0.0421 and 0.0003, respectively). The level of TIMP-1 in BALF of PAE group was significantly lower than that in other bacteria group (P = 0.0324). The ratio of TIMP-l/MMP-9 in BALF of BE group was significantly lower than that in control group (P = 0.0000), and this ratio of PAE group was significantly lower than those in both other bacteria group and control group (P = 0.0026 and 0.0000, respectively). Conclusion: PAE infection in BE patients can suppress the expression of TIMP-1 and stimulate the expression of MMP-9, and thus make the disease even worse.
Li J.,Zhejiang University |
Teng L.,Zhejiang University |
Jiang H.,First Hospital of Jiaxing
Asia-Pacific Journal of Clinical Oncology | Year: 2014
Aim: Vascular endothelial growth factor (VEGF) stimulates angiogenesis, increases vascular permeability and seems to correlate to aggressiveness of tumors. Increased expression of VEGF has been observed in papillary thyroid carcinoma (PTC). Thyroid stimulating hormone (TSH) is the major thyroid hormone, but its relationship with VEGF has seldom been studied. Therefore, we explored whether the immunohistochemical expression of VEGF was related to the serum TSH level. Methods: VEGF expression was analyzed in 43 archived PTC specimens using immunohistochemistry, with adjacent normal thyroid tissues used as controls. Serum TSH was measured by immunoradiometric assay before surgery and correlated to VEGF expression. Results: Positive VEGF expression was found in 90% of 43 PTC tissue specimens, but not in normal tissue. The serum TSH levels revealed a positive correlation with VEGF expression, R=0.592 (P<0.01). Conclusions: TSH is a major thyroid hormone. Whether TSH promotes the progression of PTC is not known but the serum TSH levels reveal a positive correlation with VEGF expression. © 2013 Wiley Publishing Asia Pty Ltd.
Huang B.,Zhejiang University |
Sun K.,Zhejiang University |
Zhu Z.,First Hospital of Jiaxing |
Zhou C.,Tongde Hospital of Zhejiang Province |
And 3 more authors.
Clinical Radiology | Year: 2013
Aim To test the hypothesis that the oximetry-derived perfusion index (PI) recorded in the index finger may provide earlier objective evidence for correct positioning of the needle tip during computed tomography (CT)-guided thoracic sympathetic blockade than skin temperature in palmar hyperhidrosis. Materials and methods Forty-four CT-guided thoracic sympathetic blockades were prospectively performed in both hands of 22 patients. Prior to chemical blockade, PI and skin temperature were recorded at 1 min intervals until 20 min after lidocaine injection. Repeated measures analysis of variance was used to assess the potency of PI and skin temperature over time. Using a 100% increase in the PI as the threshold and symptom relief within 20 min as the reference standard, the sensitivity and specificity for predicting a successful injection were calculated. Results Thirty-seven (84%) sympathetic blockade procedures were clinically successful. For successful cases, the PI increased as early as 1 min after the blockade of the sympathetic chain (p < 0.05), whereas the skin temperature showed statistical significance 1 min later (p < 0.05). The PI can be used to indicate a successful CT-guided sympathetic blockade with 97% sensitivity and 100% specificity 3 min after lidocaine injection. Conclusion The PI, a more marked and sensitive indicator than changes in skin temperature, can be used to indicate a successful CT-guided sympathetic blockade with satisfying sensitivity and specificity, 3 min after lidocaine injection in palmar hyperhidrosis. © 2013 The Royal College of Radiologists. Published by Elsevier Ltd. All rights reserved.
Shen P.,Jiaxing University |
Zong Y.-M.,Jiaxing University |
Shu J.,Jiaxing University |
Shi Y.-C.,Jiaxing University |
And 4 more authors.
Critical Care | Year: 2013
H7N9 influenza is a new emerging infection and has high mortality. Both chest radiography and computed tomography (CT) had some limitations in assessing such patients. We performed daily lung ultrasound in a patient with H7N9 influenza. Lung ultrasound and lung ultrasound score showed high consistency with CT and the progression of pneumonia. Ultrasound can be adjutant to chest radiography and CT in caring for patients with H7N9 influenza. © 2013 BioMed Central Ltd.
Zhang H.,First Hospital of Jiaxing |
Chen L.,First Hospital of Jiaxing |
Bu H.-Q.,Tongde Hospital of Zhejiang Province |
Yu Q.-J.,First Hospital of Jiaxing |
And 5 more authors.
Oncology Reports | Year: 2015
Emodin, a natural anthraquinone derivative isolated from Rheum palmatum, has been reported to inhibit the growth of pancreatic cancer cells through different modes of action; yet, the detailed mechanism remains unclear. In the present study, we hypothesized that emodin exerts its antitumor effect by participating in the regulation of the DNA methylation level. Our research showed that emodin inhibited the growth of pancreatic cancer PANC-1 cells in a dose- and time-dependent manner. Dot-blot results showed that 40 μM emodin significantly inhibited genomic 5mC expression in the PANC-1 cells, and mRNA-Seq showed that different concentrations of emodin could alter the gene expression profile in the PANC-1 cells. BSP confirmed that the methylation levels of P16, RASSF1A and ppENK were decreased, while concomitantly the unmethylated status was increased. RT-PCR and western blotting results confirmed that the low expression or absence of expression of mRNA and protein in the PANC-1 cells was re-expressed following treatment with emodin. In conclusion, our study for the first time suggests that emodin inhibits pancreatic cancer cell growth, which may be related to the demethylation of tumor-suppressor genes. The related mechanism may be through the inhibition of methyltransferase expression.