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Harbin, China

Wang C.,Harbin Medical University | Li F.,Harbin Medical University | Guan Y.,Hangzhou Hospital of Zhejiang CAPF | Zhu L.,Harbin Medical University | And 3 more authors.
Journal of Stroke and Cerebrovascular Diseases | Year: 2014

This study aimed to investigate the combination effects of bone marrowstromal cells (BMSCs) and oxiracetam for ischemic stroke. Forty Sprague Dawley female rats (220 ± 20 g) were subjected to a 2-hour ischemic middle cerebral artery occlusion (MCAO)-24 hours reperfusion model. The rats were randomly divided into 4 groups. Rats from BMSCs group, oxiracetamgroup, and BMSCs1 oxiracetam group accepted injection of BMSCs (3 × 106 cells), oxiracetam (800 mg/kg), and BMSCs+oxiracetam, respectively. Rats fromcontrol group did not receive any interventions after ischemia reperfusion. The neurologic function was examined bymodified neurological severity scores (mNSS). B-cell lymphoma 2 (Bcl-2) expression and apoptosis were detected by immunohistochemistry and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining. The mNSS was decreased in all treatment groups and that in BMSCs 1 oxiracetam group was lower than BMSCs group and oxiracetam group (P < 05). The expression of Bcl-2 was unregulated in all treatment groups (P < 05), and similarly, the expression of Bcl-2 in BMSCs 1 oxiracetam group was higher than BMSCs group and oxiracetam group (P < 05). Control group displayed more TUNEL-positive cells than the treatment groups, and BMSCs + oxiracetam group displayed less apoptotic cells than BMSCs group or oxiracetam group (P < 05). Transplantation of BMSCs can promote the recovery of neurologic function in MCAO rats, and the effect of BMSCs combined with oxiracetam was better than the either one. Upregulation of Bcl-2 resulting in a decrease of apoptosis may be one of the mechanisms ofBMSCs treatment for cerebral ischemic stroke. © 2014 by National Stroke Association. Source


Liu G.,Guangdong Medical College | Liu G.,CAS Tianjin Institute of Industrial Biotechnology | Wang H.,Harbin Medical University | Liu J.,First Hospital of Harbin | And 7 more authors.
NeuroMolecular Medicine | Year: 2014

Large-scale genomewide association studies have reported that the CLU rs11136000 polymorphism is significantly associated with Alzheimer's disease (AD) in people of Caucasian ancestry. Recently, this association was investigated in Asian populations (Chinese, Japanese, and Korean). However, these studies reported either a weak association or no association between the rs11136000 polymorphism and AD. We believe that this discrepancy may be caused by the relatively small sample size of the previous studies and the genetic heterogeneity of the rs11136000 polymorphism in AD among different populations. For this study, we searched the PubMed and AlzGene databases. We selected 18 independent studies (6 studies of Asian populations and 12 of populations of Caucasian ancestry) that evaluated the association between the rs11136000 polymorphism and AD using a case-control experimental design. We evaluated the genetic heterogeneity of the rs11136000 polymorphism in Caucasian and Asian populations. We then investigated the rs11136000 polymorphism by a meta-analysis in Asian populations using allele, dominant, and recessive models. We identified a significant association between rs11136000 and AD with the allele model (P = 2.00 × 10-4) and the dominant model (P = 5.00 × 10-3). Meanwhile, a similar genetic risk of the rs11136000 polymorphism in AD was observed in Asian and Caucasian populations. Further meta-analysis in pooled Asian and Caucasian populations indicated a more significant association with the allele (P = 8.30 × 10-24), dominant (P = 4.46 × 10-17), and recessive (P = 3.92 × 10-12) models. Collectively, our findings from this meta-analysis indicate that the effect of the CLU rs11136000 polymorphism on AD risk in Asian cohorts (Chinese, Japanese, and Korean) is consistent with the protective effect observed in Caucasian AD cohorts. © 2013 Springer Science+Business Media New York. Source


Liu A.Y.,Harbin Medical University | Liu D.-G.,Capital Medical University | Du Y.-J.,Harbin Medical University | Pei F.-H.,Harbin Medical University | And 8 more authors.
Cancer Biology and Therapy | Year: 2014

NPRL2 is a tumor suppressor gene involved in the progression of human cancer. The present study investigated whether NPRL2 expression correlates with colorectal cancer (CRC) progression. Colorectal tissue and peripheral blood samples were obtained from 62 patients with CRC, 38 patients with colorectal adenomas and 51 normal controls. NPRL2 mRNA levels in tissue samples and blood were measured using quantitative real-time PCR. NPRL2 protein expression was determined by immunohistochemistry. NPRL2 protein expression in CRCs was significantly lower than in the adenomas or normal colorectal tissue. NPRL2 mRNA expression was significantly decreased in adenomas compared with normal controls (P < 0.0001) and it was further decreased in colorectal tumors compared with adenomas (P < 0.0001). NPRL2 mRNA levels expression correlated with tumor stage. In addition, NPRL2 mRNA levels in the blood correlated with the levels detected in tumors. Furthermore, receiver operating characteristic (ROC) analysis showed that NPRL2 expression in blood could distinguish colorectal adenomas and CRCs from normal controls. NPRL2 mRNA expression in CRC tumor tissues and peripheral blood correlated with colorectal tumor progression. Based on our findings, we can conclude that NPRL2 mRNA blood levels could be a potentially useful marker for the detection of early stage adenomas and CRCs. © 2014 Landes Bioscience. Source


Hongmei Y.,Capital Medical University | Zhe W.,Wei Fang Peoples Hospital | Jing W.,First Hospital of Harbin | Daokui W.,Wei Fang Peoples Hospital | And 2 more authors.
Journal of Clinical Neuroscience | Year: 2012

We report a patient who developed delayed cerebrospinal fluid (CSF) rhinorrhea 11 years after gamma knife radiosurgery for a growth hormone (GH)-secreting adenoma. The treatment dose was 18 Gy for the tumor margin (50% isodose). One year later, an MRI of the head revealed that the tumor size had decreased. Eleven years later, the patient developed CSF rhinorrhea from the left nostril. Subsequent MRI examination revealed complete remission of the tumor in the sella turcica and the empty sella. The patient was admitted for direct endoscopic surgical repair of the skull base. We suggest that the cause of the CSF rhinorrhea is secondary empty sella. The other potential causes may be the original invasiveness of the tumor or delayed radiation damage to the mucous membranes of the skull. Long-term follow-up is required to monitor recurrence of CSF rhinorrhea. © 2012 Elsevier Ltd. All rights reserved. Source


Liu B.-R.,Harbin Medical University | Kong L.-J.,Harbin Medical University | Song J.-T.,Harbin Medical University | Liu W.,Harbin Medical University | And 2 more authors.
Journal of Laparoendoscopic and Advanced Surgical Techniques | Year: 2012

Background: NOTES cholecystectomy has become one of the hottest areas of research. But most of the cases need the assistance of the laparoscope. This study is conducted to evaluate the feasibility and safety of a newly proposed operative method-functional cholecystectomy by pure NOTES. Materials and Methods: The functional cholecystectomy was performed on eight female miniature pigs. An incision was made on the vaginal wall, and an endoscope was inserted into the peritoneal cavity to create a pneumoperitoneum to expose the intra-abdominal viscera, gallbladder, and cystic duct. The cystic duct was isolated and closed with a clip. Then, an injection needle was inserted into the gallbladder to suck up the bile. After the gallbladder was washed with saline, an incision was made on the wall of the gallbladder, and the tip of the endoscope was inserted into the gallbladder cavity. After the endoscope was withdrawn, the gallbladder incision was closed with clips in four pigs and was suspended in the other four pigs. The vaginal incision was closed with clips. All the animals were closely monitored and euthanized 28 days after the procedure. Necropsy was performed. Results: The functional cholecystectomy was successfully completed in all eight pigs. No severe intraoperative complications occurred. The animals recovered well postoperatively. At necropsy, no macroscopic signs of intraperitoneal infection or bile leakage in the peritoneal cavity were observed, and the clips were still present on the cystic duct in a good position in all cases. The gallbladder incision healed, with no sign of bile leakage or injury to the adjacent organs. Conclusions: We successfully performed the functional cholecystectomy by transvaginal approach on pigs, which appears to be feasible, safe, and convenient. Functional cholecystectomy provides a new fitting path to pure NOTES. © 2012 Mary Ann Liebert, Inc. Source

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