Harbin, China
Harbin, China

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Bai R.-J.,Peking University | Li J.-P.,Harbin Medical University | Ren S.-H.,First Hospital of Harbin | Jiang H.-J.,Harbin Medical University | And 4 more authors.
Hepatobiliary and Pancreatic Diseases International | Year: 2014

BACKGROUND The peripheral morphologic characteristics of hepatocellular carcinoma (HCC) reflect tumor growth patterns. Computed tomography (CT) perfusion is a new method to analyze hemodynamic changes in tissues. We assessed the relationship between CT perfusion and histopathologic findings in the periphery of HCC lesions. METHODS Non-contrast CT, enhanced dual-phase CT, and CT perfusion were performed on 77 subjects (47 patients and 30 controls). Based on the imaging findings of enhanced dual-phase CT, the tumor edges were classified into three types: type I (sharp); type II (blurry); and type III (mixed). The CT perfusion parameters included hepatic blood flow, hepatic arterial fraction, hepatic arterial perfusion, and hepatic portal perfusion. The tissue sections from resected specimens were subjected to routine hematoxylin and eosin staining and immunohistochemical staining for CD34. The correlations between microvessel density (MVD) and the CT perfusion parameters were analyzed using Pearson's product-moment correlation coefficient. Changes in the perfusion parameters in tumor edges of different tumor types were evaluated. RESULTS Type I (sharp): the pathologic findings showed fibrous connective tissue capsules in the tumor edges, and an MVD >30/mm2. Type II (blurry): the histology showed that the edges were clear with no capsules and an MVD >bsupesup. Type III (mixed): the pathology was similar to that of types I and II, and an MVD >bsupesup. Hepatic blood flow, hepatic arterial fraction, hepatic arterial perfusion, and hepatic portal perfusion were significantly increased in the tumor edges of HCC patients compared to those of the controls (P<0.05). The correlation between CT perfusion parameters and MVD was higher in blurry tumor edges of type II than in those of types I or III. CONCLUSION CT perfusion imaging of tumor edges may be helpful in revealing histopathological features, and indirectly reflect angiogenic changes of HCCs. (Hepatobiliary Pancreat Dis Int 2014;13:612-617) © 2014 The Editorial Board of Hepatobiliary.


Liu G.,Guangdong Medical College | Liu G.,CAS Tianjin Institute of Industrial Biotechnology | Wang H.,Harbin Medical University | Liu J.,First Hospital of Harbin | And 7 more authors.
NeuroMolecular Medicine | Year: 2014

Large-scale genomewide association studies have reported that the CLU rs11136000 polymorphism is significantly associated with Alzheimer's disease (AD) in people of Caucasian ancestry. Recently, this association was investigated in Asian populations (Chinese, Japanese, and Korean). However, these studies reported either a weak association or no association between the rs11136000 polymorphism and AD. We believe that this discrepancy may be caused by the relatively small sample size of the previous studies and the genetic heterogeneity of the rs11136000 polymorphism in AD among different populations. For this study, we searched the PubMed and AlzGene databases. We selected 18 independent studies (6 studies of Asian populations and 12 of populations of Caucasian ancestry) that evaluated the association between the rs11136000 polymorphism and AD using a case-control experimental design. We evaluated the genetic heterogeneity of the rs11136000 polymorphism in Caucasian and Asian populations. We then investigated the rs11136000 polymorphism by a meta-analysis in Asian populations using allele, dominant, and recessive models. We identified a significant association between rs11136000 and AD with the allele model (P = 2.00 × 10-4) and the dominant model (P = 5.00 × 10-3). Meanwhile, a similar genetic risk of the rs11136000 polymorphism in AD was observed in Asian and Caucasian populations. Further meta-analysis in pooled Asian and Caucasian populations indicated a more significant association with the allele (P = 8.30 × 10-24), dominant (P = 4.46 × 10-17), and recessive (P = 3.92 × 10-12) models. Collectively, our findings from this meta-analysis indicate that the effect of the CLU rs11136000 polymorphism on AD risk in Asian cohorts (Chinese, Japanese, and Korean) is consistent with the protective effect observed in Caucasian AD cohorts. © 2013 Springer Science+Business Media New York.


Han W.,Hangzhou Dianzi University | Liu D.-N.,First Hospital of Harbin | Li Y.-F.,Northeast Forestry University | Zhao H.-T.,Hangzhou Dianzi University | Ren N.-Q.,Harbin Institute of Technology
International Journal of Hydrogen Energy | Year: 2015

A novel biological strategy of utilizing wheat as nutrient source for hydrogen production was developed. Wheat bran was used in the solid-state fermentations of Aspergillus awamori and Aspergillus oryzae that produce amylolytic and proteolytic enzyme streams, respectively. The resulting streams were added to wheat flour to generate nutrients-rich (glucose and free amino nitrogen (FAN)) solutions. Both glucose and FAN increased with increasing of wheat flour mass ratio from 3% to 10% (w/v) and the highest glucose (43.92 g/L) and FAN (944.53 mg/L) were obtained at wheat flour mass ratio of 10%. The wheat flour hydrolyzates were then used as the feedstock for biohydrogen production by Biohydrogenbacterium R3. The highest cumulative hydrogen production of 4.52 L was happened at glucose concentration of 33.15 g/L. However, the maximum hydrogen yield (2.34 mol H2/mol glucose) and hydrogen production (0.074 L H2/g wheat flour) were obtained at glucose concentration of 16 g/L. © 2015 Hydrogen Energy Publications, LLC.


Li H.,Harbin Medical University | Wei C.,Harbin Medical University | Gao J.,First Hospital of Harbin | Bai S.,Harbin Medical University | And 7 more authors.
Experimental Cell Research | Year: 2014

The physiological and pathological roles of dopamine D2 receptors (DR2) in the regulation of cardiovacular functions have been recognized. DR2 activation protects hypoxia/reoxygenation (H/R)-induced cardiomyocyte injury and apoptosis, and ischemic post-conditioning (PC) plays a critical role in cardioprotection as well; however the involvement of the DR2 activation in the PC-induced cardioprotection is unknown. In the present study, we found that the H/R increased the expressions of DR2 mRNA and protein in cardiomyocytes, which were significantly enhanced by PC. Bromocriptine (Bro, a DR2 agonist) further increased DR2 expression, but Haloperidol (Hal, a DR2 antagonist) reversed the Bro-induced DR2 expressions. PC protected against H/R-induced apoptosis, the rise of [Ca2+]i, the expressions of cleaved caspase-3 and -9, release of cytochrome c, and mPTP opening. In addition, PC counteracted the reduction of cell viability caused by H/R, increased the phosphorylation of ERK1/2, PI3K, Akt, GSK-3β and mitochondrial membrane potential. PC further increased Bcl-2 expression, promoted PKC-ε translocation to cell membrane, and activated the mitochondrial ATP-sensitive K channels (mKATP). Bro further enhanced the cardioprotective roles of PC, but Hal reversed these effects of Bro. Meanwhile, we found that DR2 was expressed in cell membrane and interacted with PKC-ε in PC. In conclusion, these results suggest that PC attenuates cardiomyocyte apoptosis via inhibition of mPTP opening by DR2-mediated activation of ERK1/2, PI3K-Akt-GSK-3β and PKC-ε-mKATP. These findings provide a novel target for the treatment of ischemic cardiomyopathy. © 2014 Elsevier Inc.


Li H.,Harbin Medical University | Gao J.,Harbin Medical University | Gao J.,First Hospital of Harbin | Guo J.,Jiamusi University | And 9 more authors.
Molecular and Cellular Biochemistry | Year: 2013

Dopamine D2 receptors (DR2) are important regulators in many organs, including cardiac system. Protein kinase C (PKC) activation and translocation is associated with cardioprotection against ischemic post-conditioning (PC); however, the regulatory role of DR2 during this process has been unknown. This study hypothesized that the prevention of cardiomyocyte damage by DR2 activation is associated with PKC translocation to the cell membrane. In the present study, we found that the ischemia/reperfusion (I/R) increased the expressions of DR2 mRNA and protein, which were further enhanced by PC. Bromocriptine (DR2 agonist) up-regulated the PC-induced DR2 expressions, and Haloperidol (DR2 antagonist) reversed the increase of DR2 expressions by Bromocriptine. PC reduced I/R-induced cardiomyocytes damage, apoptosis and myocardial infarct size, and improved cardiac function. Compared with PC, Bromocriptine further enhanced the cardioprotective roles of PC, but Haloperidol canceled the protection effect of Bromocriptine. PC up-regulated PKC-ε translocation in the particulate fraction, which was further strengthened by Bromocriptine but canceled by Haloperidol. In the cytosolic fraction, the changes of the PKC-ε translocation were opposite to the particulate fraction. These findings suggest that DR2 activation provides cardioprotection via promoting PC-induced translocation of PKC-ε. © 2013 Springer Science+Business Media New York.


PubMed | Capital Medical University, Harbin Medical University, Second Affiliated Hospital of Harbin Medical University and First Hospital of Harbin
Type: Journal Article | Journal: Cancer biology & therapy | Year: 2014

NPRL2 is a tumor suppressor gene involved in the progression of human cancer. The present study investigated whether NPRL2 expression correlates with colorectal cancer (CRC) progression. Colorectal tissue and peripheral blood samples were obtained from 62 patients with CRC, 38 patients with colorectal adenomas and 51 normal controls. NPRL2 mRNA levels in tissue samples and blood were measured using quantitative real-time PCR. NPRL2 protein expression was determined by immunohistochemistry. NPRL2 protein expression in CRCs was significantly lower than in the adenomas or normal colorectal tissue. NPRL2 mRNA expression was significantly decreased in adenomas compared with normal controls (P<0.0001) and it was further decreased in colorectal tumors compared with adenomas (P<0.0001). NPRL2 mRNA levels expression correlated with tumor stage. In addition, NPRL2 mRNA levels in the blood correlated with the levels detected in tumors. Furthermore, receiver operating characteristic (ROC) analysis showed that NPRL2 expression in blood could distinguish colorectal adenomas and CRCs from normal controls. NPRL2 mRNA expression in CRC tumor tissues and peripheral blood correlated with colorectal tumor progression. Based on our findings, we can conclude that NPRL2 mRNA blood levels could be a potentially useful marker for the detection of early stage adenomas and CRCs.


Wang C.,Harbin Medical University | Li F.,Harbin Medical University | Guan Y.,Hangzhou Hospital of Zhejiang CAPF | Zhu L.,Harbin Medical University | And 3 more authors.
Journal of Stroke and Cerebrovascular Diseases | Year: 2014

This study aimed to investigate the combination effects of bone marrowstromal cells (BMSCs) and oxiracetam for ischemic stroke. Forty Sprague Dawley female rats (220 ± 20 g) were subjected to a 2-hour ischemic middle cerebral artery occlusion (MCAO)-24 hours reperfusion model. The rats were randomly divided into 4 groups. Rats from BMSCs group, oxiracetamgroup, and BMSCs1 oxiracetam group accepted injection of BMSCs (3 × 106 cells), oxiracetam (800 mg/kg), and BMSCs+oxiracetam, respectively. Rats fromcontrol group did not receive any interventions after ischemia reperfusion. The neurologic function was examined bymodified neurological severity scores (mNSS). B-cell lymphoma 2 (Bcl-2) expression and apoptosis were detected by immunohistochemistry and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining. The mNSS was decreased in all treatment groups and that in BMSCs 1 oxiracetam group was lower than BMSCs group and oxiracetam group (P < 05). The expression of Bcl-2 was unregulated in all treatment groups (P < 05), and similarly, the expression of Bcl-2 in BMSCs 1 oxiracetam group was higher than BMSCs group and oxiracetam group (P < 05). Control group displayed more TUNEL-positive cells than the treatment groups, and BMSCs + oxiracetam group displayed less apoptotic cells than BMSCs group or oxiracetam group (P < 05). Transplantation of BMSCs can promote the recovery of neurologic function in MCAO rats, and the effect of BMSCs combined with oxiracetam was better than the either one. Upregulation of Bcl-2 resulting in a decrease of apoptosis may be one of the mechanisms ofBMSCs treatment for cerebral ischemic stroke. © 2014 by National Stroke Association.


Liu A.Y.,Harbin Medical University | Liu D.-G.,Capital Medical University | Du Y.-J.,Harbin Medical University | Pei F.-H.,Harbin Medical University | And 8 more authors.
Cancer Biology and Therapy | Year: 2014

NPRL2 is a tumor suppressor gene involved in the progression of human cancer. The present study investigated whether NPRL2 expression correlates with colorectal cancer (CRC) progression. Colorectal tissue and peripheral blood samples were obtained from 62 patients with CRC, 38 patients with colorectal adenomas and 51 normal controls. NPRL2 mRNA levels in tissue samples and blood were measured using quantitative real-time PCR. NPRL2 protein expression was determined by immunohistochemistry. NPRL2 protein expression in CRCs was significantly lower than in the adenomas or normal colorectal tissue. NPRL2 mRNA expression was significantly decreased in adenomas compared with normal controls (P < 0.0001) and it was further decreased in colorectal tumors compared with adenomas (P < 0.0001). NPRL2 mRNA levels expression correlated with tumor stage. In addition, NPRL2 mRNA levels in the blood correlated with the levels detected in tumors. Furthermore, receiver operating characteristic (ROC) analysis showed that NPRL2 expression in blood could distinguish colorectal adenomas and CRCs from normal controls. NPRL2 mRNA expression in CRC tumor tissues and peripheral blood correlated with colorectal tumor progression. Based on our findings, we can conclude that NPRL2 mRNA blood levels could be a potentially useful marker for the detection of early stage adenomas and CRCs. © 2014 Landes Bioscience.


Liu B.-R.,Harbin Medical University | Kong L.-J.,Harbin Medical University | Song J.-T.,Harbin Medical University | Liu W.,Harbin Medical University | And 2 more authors.
Journal of Laparoendoscopic and Advanced Surgical Techniques | Year: 2012

Background: NOTES cholecystectomy has become one of the hottest areas of research. But most of the cases need the assistance of the laparoscope. This study is conducted to evaluate the feasibility and safety of a newly proposed operative method-functional cholecystectomy by pure NOTES. Materials and Methods: The functional cholecystectomy was performed on eight female miniature pigs. An incision was made on the vaginal wall, and an endoscope was inserted into the peritoneal cavity to create a pneumoperitoneum to expose the intra-abdominal viscera, gallbladder, and cystic duct. The cystic duct was isolated and closed with a clip. Then, an injection needle was inserted into the gallbladder to suck up the bile. After the gallbladder was washed with saline, an incision was made on the wall of the gallbladder, and the tip of the endoscope was inserted into the gallbladder cavity. After the endoscope was withdrawn, the gallbladder incision was closed with clips in four pigs and was suspended in the other four pigs. The vaginal incision was closed with clips. All the animals were closely monitored and euthanized 28 days after the procedure. Necropsy was performed. Results: The functional cholecystectomy was successfully completed in all eight pigs. No severe intraoperative complications occurred. The animals recovered well postoperatively. At necropsy, no macroscopic signs of intraperitoneal infection or bile leakage in the peritoneal cavity were observed, and the clips were still present on the cystic duct in a good position in all cases. The gallbladder incision healed, with no sign of bile leakage or injury to the adjacent organs. Conclusions: We successfully performed the functional cholecystectomy by transvaginal approach on pigs, which appears to be feasible, safe, and convenient. Functional cholecystectomy provides a new fitting path to pure NOTES. © 2012 Mary Ann Liebert, Inc.


Zhang H.,First Hospital of Harbin | Zhang H.,Affiliated Hospital to Academy of Military Medical science | Zhang B.,Affiliated Hospital to Academy of Military Medical science | Zhang B.,Academy of Military Medical science | And 6 more authors.
Cell Biochemistry and Function | Year: 2012

Mesenchymal stem cells (MSCs) are multipotent cells traditionally derived from bone marrow (BM). They have been demonstrated to be widely applied in tissue regeneration and cellular therapy. As an alternative to BM, an umbilical cord (UC) is considered as a potential source of MSCs. Here, we showed that human UC-MSCs were easily isolated by a single enzymatic digestion and characteristic of plastic adherence and fibroblast-like morphology. UC-MSCs isolation was successful in 15 of 15 samples. The colony-forming unit-fibroblast frequency was obtained 54±1.33 from 103 UC-MSCs at passage 3, and the doubling time was (24.15±0.49)h. Almost 1010 UC-MSCs were largely produced in about 30days. By flow cytometry analysis, the adherent cells displayed an abundant presence of CD73, CD90 and CD105 and absence of CD34, CD45 and HLA-DR. When cultured in differentiation media, they can be differentiated into adipocytes, osteocytes and chondrocytes. RT-PCR reactions confirmed that their multidifferentiation related genes were positive. Moreover, stem cell-related transcription factors Nanog, Oct-4 and Sox-2 were positively expressed in UC-MSCs. On the basis of these findings, the single enzyme method is a good method to obtain large-scale production of MSCs from whole human UC in a short time, and the UC can be considered as a novel and convenient source of adult MSCs displaying high expansion potential and primitive pluripotent stem cells. © 2012 John Wiley & Sons, Ltd.

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