First Central Hospital of Baoding
First Central Hospital of Baoding
Liu Y.,Hebei Medical University |
Wang X.,First Central Hospital of Baoding |
Jia Y.,Hebei Medical University
International Journal of Molecular Medicine | Year: 2017
The aim of this study was to investigate the effects of bufalin on the mammalian target of rapamycin (mTOR/p70S6 kinase (p70S6K) signaling pathway and cell apoptosis in orthotopically transplanted tumors in nude mice. The mice were inoculated with human esophageal squamous cell carcinoma (ESCC) ECA109 cells in order to establish a model of orthotopicall transplanted ESCC tumors. The mice are administered low, medium and high doses of bufalin (0.5, 1.0 and 1.5 mg/kg) or rapamycin, or a combination of both. After the tumors were removed, the mRNA expression levels of mTOR, p70S6K, eukaryotic translation initiation factor 4E-binding protein 1 (4EBP1), cellular inhibitor of apoptosis protein 1 (cIAP1) and caspase-3 were detected by RT-PCR. In addition, we performed western blot analysis and immunohistochemical analysis to determine the protein expression of mTOR, p70S6K, 4EBP1, cIAP1, active caspase-3, Bcl-2 and Bad in the tumor tissue. The results revealed that bufalin exerted a significant anti-Tumor effect in the nude mice with ESCC orthotopically transplanted tumors. This was shown by the decrease in the expression of mTOR, p70S6K and 4EBP1, which suggested that bufalin may possibly be used to inhibit tumor growth via the inhibition of the activation of p70S6K and 4EBP1. We also found that bufalin decreased the expression of cIAP1 and Bcl-2, and increased that of active caspase-3 and Bad, thus indicating that bufalin promoted apoptosis. Thus, our findings suggest that bufalin promotes tumor cell apoptosis, and this may be one of the important anti-Tumor mechanisms of action of bufalin.
Wang Q.,First Central Hospital of Baoding |
Xiao Y.,252nd Hospital of the PLA |
Liu T.,252nd Hospital of the PLA |
Yuan H.,252nd Hospital of the PLA |
Li C.,252nd Hospital of the PLA
Molecular Medicine Reports | Year: 2017
The present study investigated the pharmacody-namic role and therapeutic mechanism of demethylzeylasteral in the suppression of inflammation in a rat model of unilateral ureteral obstruction and reduction in nuclear factor (NF)- κB pathway activity. The rats in the unilateral ureteral obstruction model were treated with 30-120 mg/kg demethylzeylasteral for 8 weeks. The activities of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and caspase-3/9, and the protein expression levels of cyclooxygenase (COX)-2 and intercellular adhesion molecule-1 (ICAM-1) and NF-κB p65 were analyzed using ELISA kits and western blot analyses, respectively. Compared with the rats in the unilateral ureteral obstruction model group, demethylzeylasteral treatment markedly inhibited the increased concentrations of serum creatinine and blood urea nitrogen, urinary protein/creatinine ratio, and concentrations of high-density lipoprotein and low-density lipoprotein cholesterol, and prevented kidney damage. In addition demethylzeylasteral inhibited the levels of TNF-α andIL-6 and suppressed the protein expression levels of COX-2 and ICAM-1 in the kidneys of the rats in the unilateral ureteral obstruction model. Demethylzeylasteral also significantly suppressed the protein expression of NF-κB p65. The results of the present study suggested that demethylzeylasteral unilateral ureteral obstruction and inhibited inflammation via inhibiting the activation of COX-2, ICAM-1 and NF-κB p65, and suppressing the activities of caspase-3/9 in rats with unilateral ureteral obstruction.
Zhang H.,First Central Hospital of Baoding |
Ke B.,Tianjin Medical University |
Liang H.,Tianjin Medical University
Chinese Journal of Clinical Oncology | Year: 2016
STMN1 is a microtubule-destabilizing protein that regulates cell cycle by phosphorylation and dephosphorylation. It plays an important role in the proliferation and differentiation of cells, in addition to the tumorigenesis. This protein is highly expressed in a wide variety of human cancers, including leukemia and multiple types of solid tumors. The relationship between STMN1 and gastric cancer has recently been investigated. Studying STMN1 in gastric cancer is important. A number of studies have suggested that overexpression of STMN1 can affect the therapeutic response of docetaxel, an anti-microtubule drug. This review summarizes the role of STMN1 in gastric carcinogenesis, development, prognosis, and treatment. The relationship between STMN1 and clinical pathology and its regulation pathways is also investigated.
Liu X.,Oregon Health And Science University |
Liu X.,First Central Hospital of Baoding |
Zhu M.,Oregon Health And Science University |
Streiff C.,Oregon Health And Science University |
And 2 more authors.
Journal of Ultrasound in Medicine | Year: 2016
Objectives - This study tested the accuracy of new 4-dimensional fetal echocardiography to evaluate left ventricular (LV) mass in an experimental model of fetal myocardial hypertrophy. Methods - Ten fresh rabbit hearts were studied. Fetal myocardial hypertrophy was simulated by fixing different amounts of myocardial tissue to the LV epicardium. A small latex balloon was mounted on vinyl tubing and fixed within each LV cavity. The proximal end of the tube was attached to a pulsatile pump apparatus. The pump was calibrated to deliver stroke volumes of 2 and 4 mL at stroke rates of 60 and 120 beats per minute (bpm). Four-dimensional data were acquired and analyzed with quantification software. Reference values for LV mass were determined by the displacement method. Results - Echo-derived measurements of LV mass showed good correlations with reference values at all stroke rates and stroke volumes: at 2 mL and 60 bpm, r = 0.95; at 2 mL and 120 bpm, r = 0.95; at 4 mL and 60 bpm, r = 0.93; and at 4 mL and 120 bpm, r = 0.95 (P< .01 for all values). There was also excellent interobserver (r = 0.98; mean difference of -0.32 g; -4.4% of the mean) and intraobserver (r = 0.98; mean difference of -0.28 g; -3.8% of the mean) agreement. Conclusions - In this controlled in vitro study, high-resolution 4-dimensional echocardiography was shown to accurately assess LV mass and have the potential to evaluate fetal myocardial hypertrophy. © 2016 by the American Institute of Ultrasound in Medicine.
Wang S.,First Central Hospital of Baoding |
Ren D.,First Central Hospital of Baoding
Molecular Medicine Reports | Year: 2016
Allicin is a major component of garlic, extracted as an oily liquid. The present study was designed to investigate the beneficial effects of allicin on traumatic spinal cord injury (TSCI) in mice, and whether the effects are mediated via regulation of the heat shock protein 70 (HSP70), v-akt murine thymoma viral oncogene homolog 1 (Akt) and inducible nitric oxide synthase (iNOS) pathways. Adult BALB/c mice (30-40 g) received a laminectomy at the T9 vertebral level as a model of TSCI. In the present study, treatment of the TSCI mice with allicin significantly increased their Basso, Beattie and Bresnahan (BBB) scores (P<0.01) and reduced the spinal cord water content (P<0.01). This protective effect was associated with the inhibition of oxidative stress and inflammatory responses in TSCI mice. Western blot analysis demonstrated that allicin increased the protein levels of HSP70, increased the phosphorylation of Akt and reduced the iNOS protein expression levels in TSCI mice. Additionally, treatment with allicin significantly reduced the levels of ROS and enhanced the NADH levels in TSCI mice. Collectively, these data demonstrate that the effects of allicin on TSCI are mediated via regulation of the HSP70, Akt and iNOS pathways in mice.
Zhang B.,First Central Hospital of Baoding |
Zhao X.,First Central Hospital of Baoding |
Zhang J.,First Central Hospital of Baoding |
Jia W.,First Central Hospital of Baoding |
And 2 more authors.
Cancer Research and Clinic | Year: 2016
Objective: To investigate the expression and clinical significance of apoptotic protease activating factor 1 (Apaf-1) and bax in prostate cancer (PCa) and benign prostatic hyperplasia (BPH). Methods: Immunohistochemistry was used to detect the expression of Apaf-1 and bax in the tissues from 45 PCa patients and 60 BPH patients. Results: The positive rates of Apaf-1 and bax in PCa tissues were 22.22 % (10/45) and 20.00 % (9/45), respectively, while those in BPH tissues were 48.33 % (29/60) and 46.67 % (28/60). There was a statistically significant difference in the expressions of Apaf-1 and bax between two groups (P < 0.05). The expressions of Apaf-1 and bax were not correlated with the age of patients and distant metastasis (P > 0.05), but they were correlated with the pathological grade and clinical stage of PCa (P < 0.05). The expressions of Apaf-1 and bax in PCa tissues were lower than those in BPH tissues. There was a positive correlation between the expression of Apaf-1 and bax (r = 0.535, P < 0.01). Conclusion: Apaf-1 and bax might be correlated with the carcinogenesis and development of PCa. © Copyright 2016 by the Chinese Medical Association.
Wang K.,Hebei University |
Wang Y.,Hebei University |
Yan X.,Hebei University |
Chen H.,Hebei University |
And 6 more authors.
Bioorganic and Medicinal Chemistry Letters | Year: 2012
A series of novel naphthalimide derivatives modified with various hydroxyl-alkylamines at 4-position have been synthesized. Their DNA binding properties were investigated by UV-Vis, fluoescence, and circular dichroism (CD) spectroscopies and thermal denaturation. The results showed that compounds 3a-e as the DNA intercalator exhibited middle binding affinities with Ct-DNA. The anticancer activities of 3a-e were preliminarily evaluated, compounds 3c and 3e exhibited potent anticancer activities against Bel-7402 cell line with IC 50 values of 5.57 and 9.17 μM, respectively. More interestingly, enhancement of the fluorescence emission was found in the complexes of 3a-e with Ct-DNA, especially for 3c. This would make these compounds as potential DNA staining agents. © 2011 Elsevier Ltd. All rights reserved.
Zhou B.,First Central Hospital of Baoding |
Ma Q.,First Central Hospital of Baoding |
Chen H.,First Central Hospital of Baoding |
Zhao W.,First Central Hospital of Baoding |
And 7 more authors.
Cancer Research and Clinic | Year: 2015
Objective To detect the expression and clinical significance of forkhead box Ml (FOXM1) and S-phase kinase associated protein 2 (Skp2) in basal-like breast carcinoma (BLBC). Methods The expression of FOXM1 and Skp2 was detected in 95 cases of BLBC, 95 adjacent breast tissues and 100 cases of non-BLBC by immunohistochemistry (IHC). Rusults The result of IHC showed that the positive expression rates of FOXM1 and Skp2 in BLBC were 76.84 % (73/95) and 58.95 % (56/95), which were much higher than those in non-BLBC [57.00 % (57/100) and 40.00 % (40/100), all P < 0.016 7] and adjacent breast tissues [25.26 % (24/95) and 3.16 (3/95), P < 0.016 7]. The expression of FOXM1 and Skp2 related with lymph node metastasis and pTNM staging of BLBC (P < 0.05). The expression of FOXM1 and Skp2 in BLBC had a positive correlation (r = 0.353, P < 0.01). Conclusions FOXM1 and Skp2 are overexpressed in BLBC and may play synergistic roles in the carcinogenesis and progression of BLBC. They may be as the indications of biological behavior and prognosis of BLBC.
Ma Q.-S.,First Central Hospital of Baoding
Cancer Research and Clinic | Year: 2013
Objective: To detect the protein expressions of FoxM1 and c-myc in basal-like breast carcinoma and explore their correlation. Methods: The expression of FoxM1 and c-myc was detected in 66 cases of BLBC, 70 cases of non-BLBC and 66 cases of normal adjacent breast tissue by immunohistochemistry. The relationship of them was analyzed. Rusults: The expression rates of FoxM1 and c-myc protein in BLBC [77.3% (51/66), 71.2% (47/66)] was significant increased than that in normal breast tissue [13.6% (9/66), 22.7% (15/66)], and higher than that in non-BLBC [60.0% (42/70), 54.3% (38/70)]. The expression of FoxM1 and c-myc positively correlated with lymph nodes metastasis and TNM staging of BLBC (P < 0.05). Positive expressions correlation could be found between the expression of FoxM1 and that of c-myc as well (r = 0.294, P < 0.05). Conclusion: FoxM1 and c-myc may play important roles in the carcinogenesis and development of BLBC and the study supports the positive feedback effect between the expressions of FoxM1 and c-myc furthermore.
PubMed | Hebei Medical University and First Central Hospital of Baoding
Type: | Journal: Scientific reports | Year: 2016
Obesity-induced kidney injury contributes to albuminuria, which is characterized by a progressive decline in renal function leading to glomerulosclerosis and renal fibrosis. Matrix metalloproteinases (MMPs) modulate inflammation and fibrosis by degrading a variety of extracellular matrix and regulating the activities of effector proteins. Abnormal regulation of MMP-12 expression has been implicated in abdominal aortic aneurysm, atherosclerosis, and emphysema, but the underlying mechanisms remain unclear. The present study examined the function of MMP-12 in glomerular fibrogenesis and inflammation using apo E(-/-) or apo E(-/-)MMP-12(-/-) mice and maintained on a high-fat-diet (HFD) for 3, 6, or 9 months. MMP-12 deletion reduced glomerular matrix accumulation, and downregulated the expression of NADPH oxidase 4 and the subunit-p67(phox), indicating the inhibition of renal oxidative stress. In addition, the expression of the inflammation-associated molecule MCP-1 and macrophage marker-CD11b was decreased in glomeruli of apo E(-/-)MMP-12(-/-) mice fed HFD. MMP-12 produced by macrophages infiltrating into glomeruli contributed to the degradation of collagen type IV and fibronectin. Crescent formation due to renal oxidative stress in Bowmans space was a major factor in the development of fibrogenesis and inflammation. These results suggest that regulating MMP-12 activity could be a therapeutic strategy for the treatment of crescentic glomerulonephritis and fibrogenesis.