Liu X.,Oregon Health And Science University |
Liu X.,First Central Hospital of Baoding |
Zhu M.,Oregon Health And Science University |
Streiff C.,Oregon Health And Science University |
And 2 more authors.
Journal of Ultrasound in Medicine | Year: 2016
Objectives - This study tested the accuracy of new 4-dimensional fetal echocardiography to evaluate left ventricular (LV) mass in an experimental model of fetal myocardial hypertrophy. Methods - Ten fresh rabbit hearts were studied. Fetal myocardial hypertrophy was simulated by fixing different amounts of myocardial tissue to the LV epicardium. A small latex balloon was mounted on vinyl tubing and fixed within each LV cavity. The proximal end of the tube was attached to a pulsatile pump apparatus. The pump was calibrated to deliver stroke volumes of 2 and 4 mL at stroke rates of 60 and 120 beats per minute (bpm). Four-dimensional data were acquired and analyzed with quantification software. Reference values for LV mass were determined by the displacement method. Results - Echo-derived measurements of LV mass showed good correlations with reference values at all stroke rates and stroke volumes: at 2 mL and 60 bpm, r = 0.95; at 2 mL and 120 bpm, r = 0.95; at 4 mL and 60 bpm, r = 0.93; and at 4 mL and 120 bpm, r = 0.95 (P< .01 for all values). There was also excellent interobserver (r = 0.98; mean difference of -0.32 g; -4.4% of the mean) and intraobserver (r = 0.98; mean difference of -0.28 g; -3.8% of the mean) agreement. Conclusions - In this controlled in vitro study, high-resolution 4-dimensional echocardiography was shown to accurately assess LV mass and have the potential to evaluate fetal myocardial hypertrophy. © 2016 by the American Institute of Ultrasound in Medicine.
Wang S.,First Central Hospital of Baoding |
Ren D.,First Central Hospital of Baoding
Molecular Medicine Reports | Year: 2016
Allicin is a major component of garlic, extracted as an oily liquid. The present study was designed to investigate the beneficial effects of allicin on traumatic spinal cord injury (TSCI) in mice, and whether the effects are mediated via regulation of the heat shock protein 70 (HSP70), v-akt murine thymoma viral oncogene homolog 1 (Akt) and inducible nitric oxide synthase (iNOS) pathways. Adult BALB/c mice (30-40 g) received a laminectomy at the T9 vertebral level as a model of TSCI. In the present study, treatment of the TSCI mice with allicin significantly increased their Basso, Beattie and Bresnahan (BBB) scores (P<0.01) and reduced the spinal cord water content (P<0.01). This protective effect was associated with the inhibition of oxidative stress and inflammatory responses in TSCI mice. Western blot analysis demonstrated that allicin increased the protein levels of HSP70, increased the phosphorylation of Akt and reduced the iNOS protein expression levels in TSCI mice. Additionally, treatment with allicin significantly reduced the levels of ROS and enhanced the NADH levels in TSCI mice. Collectively, these data demonstrate that the effects of allicin on TSCI are mediated via regulation of the HSP70, Akt and iNOS pathways in mice.
Zhang B.,First Central Hospital of Baoding |
Zhao X.,First Central Hospital of Baoding |
Zhang J.,First Central Hospital of Baoding |
Jia W.,First Central Hospital of Baoding |
And 2 more authors.
Cancer Research and Clinic | Year: 2016
Objective: To investigate the expression and clinical significance of apoptotic protease activating factor 1 (Apaf-1) and bax in prostate cancer (PCa) and benign prostatic hyperplasia (BPH). Methods: Immunohistochemistry was used to detect the expression of Apaf-1 and bax in the tissues from 45 PCa patients and 60 BPH patients. Results: The positive rates of Apaf-1 and bax in PCa tissues were 22.22 % (10/45) and 20.00 % (9/45), respectively, while those in BPH tissues were 48.33 % (29/60) and 46.67 % (28/60). There was a statistically significant difference in the expressions of Apaf-1 and bax between two groups (P < 0.05). The expressions of Apaf-1 and bax were not correlated with the age of patients and distant metastasis (P > 0.05), but they were correlated with the pathological grade and clinical stage of PCa (P < 0.05). The expressions of Apaf-1 and bax in PCa tissues were lower than those in BPH tissues. There was a positive correlation between the expression of Apaf-1 and bax (r = 0.535, P < 0.01). Conclusion: Apaf-1 and bax might be correlated with the carcinogenesis and development of PCa. © Copyright 2016 by the Chinese Medical Association.
Wang K.,Hebei University |
An H.,Hebei University |
Wang Y.,Hebei University |
Yan X.,Hebei University |
And 7 more authors.
Chinese Journal of Organic Chemistry | Year: 2012
A series of novel low molecular weight poly(ethylene imine) (diethylenetriamine, triethylenetetraamine and tetraethylenepentamine) modified naphthalimide derivatives at 4 position as the DNA binding agents were designed and synthesized. Their DNA binding properties were investigated by UV-Vis, fluorescence and circular dischroism (CD) spectroscopies and thermal denaturation experiment. Furthermore, the anticancer activity was evaluated against different tumor cell lines (human hepatocellular carcinoma Bel-7402, cells, human promyelocyte leukemia HL-60 cells, human lung cancer A549 cell, human cervical carcinoma Hela cell). Compound Nil exhibited potent anticancer activity against A549 cell line with IC 50 value of 3.16 μ,mol/L, which was better than the positive control drug (cisplatin). © 2012 Chinese Chemical Society & SIOC, CAS.
Wang K.,Hebei University |
Wang Y.,Hebei University |
Yan X.,Hebei University |
Chen H.,Hebei University |
And 6 more authors.
Bioorganic and Medicinal Chemistry Letters | Year: 2012
A series of novel naphthalimide derivatives modified with various hydroxyl-alkylamines at 4-position have been synthesized. Their DNA binding properties were investigated by UV-Vis, fluoescence, and circular dichroism (CD) spectroscopies and thermal denaturation. The results showed that compounds 3a-e as the DNA intercalator exhibited middle binding affinities with Ct-DNA. The anticancer activities of 3a-e were preliminarily evaluated, compounds 3c and 3e exhibited potent anticancer activities against Bel-7402 cell line with IC 50 values of 5.57 and 9.17 μM, respectively. More interestingly, enhancement of the fluorescence emission was found in the complexes of 3a-e with Ct-DNA, especially for 3c. This would make these compounds as potential DNA staining agents. © 2011 Elsevier Ltd. All rights reserved.
Zhou B.,First Central Hospital of Baoding |
Ma Q.,First Central Hospital of Baoding |
Chen H.,First Central Hospital of Baoding |
Zhao W.,First Central Hospital of Baoding |
And 7 more authors.
Cancer Research and Clinic | Year: 2015
Objective To detect the expression and clinical significance of forkhead box Ml (FOXM1) and S-phase kinase associated protein 2 (Skp2) in basal-like breast carcinoma (BLBC). Methods The expression of FOXM1 and Skp2 was detected in 95 cases of BLBC, 95 adjacent breast tissues and 100 cases of non-BLBC by immunohistochemistry (IHC). Rusults The result of IHC showed that the positive expression rates of FOXM1 and Skp2 in BLBC were 76.84 % (73/95) and 58.95 % (56/95), which were much higher than those in non-BLBC [57.00 % (57/100) and 40.00 % (40/100), all P < 0.016 7] and adjacent breast tissues [25.26 % (24/95) and 3.16 (3/95), P < 0.016 7]. The expression of FOXM1 and Skp2 related with lymph node metastasis and pTNM staging of BLBC (P < 0.05). The expression of FOXM1 and Skp2 in BLBC had a positive correlation (r = 0.353, P < 0.01). Conclusions FOXM1 and Skp2 are overexpressed in BLBC and may play synergistic roles in the carcinogenesis and progression of BLBC. They may be as the indications of biological behavior and prognosis of BLBC.
Zhang H.-Q.,First Central Hospital of Baoding |
Guo X.,First Central Hospital of Baoding |
Guo S.-Q.,First Central Hospital of Baoding |
Wang Q.,First Central Hospital of Baoding |
And 3 more authors.
European Review for Medical and Pharmacological Sciences | Year: 2016
OBJECTIVE: To study the stathmin (STMN1) expression in colorectal cancer and tumor-Adjacent normal tissue and discuss its prognostic significance in colon cancer. PATIENTS AND METHODS: STMN1 was tested with qRT-PCR in 30 samples of fresh colon cancer tissue and tumor-Adjacent issue, and with immunohistochemical SP method in 105 samples of fresh colon cancer tissue and tumor-Adjacent issue to analyze the association between its expression and clinical pathological parameters. Clinical data was combined to study the relationship between STMN1 expression and 5-year survival rate. Univariate analysis and Cox multivariate regression were performed to study the correlation between STMN1 expression and prognosis. RESULTS: The mRNA and protein level of STMN1 were significantly higher in colon cancer samples than tumor-Adjacent normal tissues (p<0.05). STMN1 expression was independent of patient age, gender or location, but significantly related to lymph node metastasis and TNM staging (p<0.05). Survival analysis by Kaplan-Meier method showed that STMN1 expression was significantly related with the survival of colon cancer patients. The median survival time of STMN1-positive patients (37.5 months) was significantly shorter than STMN1-negative patients (57.1 months, p<0.05). Cox multivariate regression indicated that STMN1 is independent prognostic factors predicting the development, invasion and metastasis of colon cancer (p<0.05). CONCLUSIONS: STMN1 overexpression in colon cancer is independently associated with improved survival and significantly related to the development of the disease. Our findings suggest that presence of a STMN1-prognosis interaction that potentially determines clinical outcome.
Ma Q.-S.,First Central Hospital of Baoding
Cancer Research and Clinic | Year: 2013
Objective: To detect the protein expressions of FoxM1 and c-myc in basal-like breast carcinoma and explore their correlation. Methods: The expression of FoxM1 and c-myc was detected in 66 cases of BLBC, 70 cases of non-BLBC and 66 cases of normal adjacent breast tissue by immunohistochemistry. The relationship of them was analyzed. Rusults: The expression rates of FoxM1 and c-myc protein in BLBC [77.3% (51/66), 71.2% (47/66)] was significant increased than that in normal breast tissue [13.6% (9/66), 22.7% (15/66)], and higher than that in non-BLBC [60.0% (42/70), 54.3% (38/70)]. The expression of FoxM1 and c-myc positively correlated with lymph nodes metastasis and TNM staging of BLBC (P < 0.05). Positive expressions correlation could be found between the expression of FoxM1 and that of c-myc as well (r = 0.294, P < 0.05). Conclusion: FoxM1 and c-myc may play important roles in the carcinogenesis and development of BLBC and the study supports the positive feedback effect between the expressions of FoxM1 and c-myc furthermore.
Cheng H.,First Central Hospital of Baoding |
Jia X.-H.,First Central Hospital of Baoding |
Zhang X.-W.,First Central Hospital of Baoding |
Zhang J.-K.,First Central Hospital of Baoding
Chinese Journal of Cancer Prevention and Treatment | Year: 2013
OBJECTIVE: To investigate the expressions and clinical significance of ABCG2 and P-gp protein in the middle-late stage gastric cancer tissues. METHODS: The immunohistochemical method was used to detect the expressions of ABCG2 and P-gp protein in 60 cases of middle-late stage gastric cancer tissues and corresponding adjacent normal tissues, and their relationships with clinicopathological features were analyzed. RESULTS: ABCG2 positive stain was mainly located in cytomembrance, and could be in cytoplasmtoo, while P-gp in cytomembrance and cytoplasm, but mainly in the latter. The positive expression rate of ABCG2 and P-gp in gastric cancer tissues was 95.0% (57/60), 85.0% (51/60), significantly higher than that in adjacent normal tissues of 81.7% (49/60), 25.0% (15/60, χ2 were 12.264 and 52.286, P were 0.007 and 0.000); Their co-expression rate in the cancer was 81.7% (49/60) and the difference was statistically significant on the expression degree (χ2=28.381, P=0.000), with a positive correlation (r=0.156), but there was no statistical significance (P=0.233). They had a poor coherence at the expression extent (Kappa=0.084), with no statistical significance (P=0.282). Their expressions were both closely correlated with the tumor TNM stage, differentiation state and invasion depth (all P=0.000), but not related to patients'age, gender, tumor size, with or without lymph node and distant metastasis (all P>0.05). CONCLUSION: ABCG2 and P-gp as a sensitive index in the testing of gastric cancer, may become a new indictor for the diagnosis of gastric cancer, evaluation of malignant degree, tumor progression, drug resistance and efficacy and prognosis.
PubMed | Hebei Medical University and First Central Hospital of Baoding
Type: | Journal: Scientific reports | Year: 2016
Obesity-induced kidney injury contributes to albuminuria, which is characterized by a progressive decline in renal function leading to glomerulosclerosis and renal fibrosis. Matrix metalloproteinases (MMPs) modulate inflammation and fibrosis by degrading a variety of extracellular matrix and regulating the activities of effector proteins. Abnormal regulation of MMP-12 expression has been implicated in abdominal aortic aneurysm, atherosclerosis, and emphysema, but the underlying mechanisms remain unclear. The present study examined the function of MMP-12 in glomerular fibrogenesis and inflammation using apo E(-/-) or apo E(-/-)MMP-12(-/-) mice and maintained on a high-fat-diet (HFD) for 3, 6, or 9 months. MMP-12 deletion reduced glomerular matrix accumulation, and downregulated the expression of NADPH oxidase 4 and the subunit-p67(phox), indicating the inhibition of renal oxidative stress. In addition, the expression of the inflammation-associated molecule MCP-1 and macrophage marker-CD11b was decreased in glomeruli of apo E(-/-)MMP-12(-/-) mice fed HFD. MMP-12 produced by macrophages infiltrating into glomeruli contributed to the degradation of collagen type IV and fibronectin. Crescent formation due to renal oxidative stress in Bowmans space was a major factor in the development of fibrogenesis and inflammation. These results suggest that regulating MMP-12 activity could be a therapeutic strategy for the treatment of crescentic glomerulonephritis and fibrogenesis.