Wang K.,Hebei University |
An H.,Hebei University |
Wang Y.,Hebei University |
Yan X.,Hebei University |
And 7 more authors.
Chinese Journal of Organic Chemistry | Year: 2012
A series of novel low molecular weight poly(ethylene imine) (diethylenetriamine, triethylenetetraamine and tetraethylenepentamine) modified naphthalimide derivatives at 4 position as the DNA binding agents were designed and synthesized. Their DNA binding properties were investigated by UV-Vis, fluorescence and circular dischroism (CD) spectroscopies and thermal denaturation experiment. Furthermore, the anticancer activity was evaluated against different tumor cell lines (human hepatocellular carcinoma Bel-7402, cells, human promyelocyte leukemia HL-60 cells, human lung cancer A549 cell, human cervical carcinoma Hela cell). Compound Nil exhibited potent anticancer activity against A549 cell line with IC 50 value of 3.16 μ,mol/L, which was better than the positive control drug (cisplatin). © 2012 Chinese Chemical Society & SIOC, CAS. Source
Wang W.,First Central Hospital of Baoding |
Gao Y.-H.,Hebei Medical University |
Wang B.,Hebei Medical University |
Diao L.-P.,Hebei Medical University |
And 2 more authors.
Chinese Journal of Cancer Prevention and Treatment | Year: 2011
OBJECTIVE: To investigate the effects of mTOR inhibitor-rapamycin on proliferation of human Burkitt lymphoma cell line Raji cells, and the molecular mechanism was analyzed. METHODS: Effect of rapamycin in different of concentration (0, 1, 5, 10, 20, 40, 50, 100 nmol/L) and for different time groups (24, 48, 72 h) on proliferation of Raji cells was detected by MTT assay. Apoptosis and cell cycle were analyzed by flow cytometry. The expression of Cyclin D1, Cyclin E, Cyclin A, p27, and Survivin protein was examined by western blot technique in the rapamycin-treated and untreated Raji cell. RESULTS: Rapamycin can inhibit the proliferation of Raji cells at concentration greater than 5 nmol/L(P<0.05), in a dose and time dependent manner. After treatment with rapamycin, the number of cells in S and G 2/M phase decreased gradually(P<0.05), but, increased in G 0/G 1 phase significantly in a dose and time dependent manners (P<0.05). However, it could not cause apoptosis in Raji cells evidently(P>0.05). The expression of Cyclin D1, p27 and Cyclin E had no obvious change(P>0.05), but the expressions of Cyclin A and Survivin protein in Raji cells were inhibited after treated with rapamycin at the concentrate of 50 nmol/L(P<0.05). CONCLUSION: Rapamycin inhibits Raji cells proliferation by cell cycle arrest in G 0/G 1, which associated with down regulate Cyclin A and Survivin. Source
Ma Q.-S.,First Central Hospital of Baoding
Cancer Research and Clinic | Year: 2013
Objective: To detect the protein expressions of FoxM1 and c-myc in basal-like breast carcinoma and explore their correlation. Methods: The expression of FoxM1 and c-myc was detected in 66 cases of BLBC, 70 cases of non-BLBC and 66 cases of normal adjacent breast tissue by immunohistochemistry. The relationship of them was analyzed. Rusults: The expression rates of FoxM1 and c-myc protein in BLBC [77.3% (51/66), 71.2% (47/66)] was significant increased than that in normal breast tissue [13.6% (9/66), 22.7% (15/66)], and higher than that in non-BLBC [60.0% (42/70), 54.3% (38/70)]. The expression of FoxM1 and c-myc positively correlated with lymph nodes metastasis and TNM staging of BLBC (P < 0.05). Positive expressions correlation could be found between the expression of FoxM1 and that of c-myc as well (r = 0.294, P < 0.05). Conclusion: FoxM1 and c-myc may play important roles in the carcinogenesis and development of BLBC and the study supports the positive feedback effect between the expressions of FoxM1 and c-myc furthermore. Source
Cheng Z.-S.,Second Military Medical University |
Yang C.-W.,Second Military Medical University |
Liu L.,First Central Hospital of Baoding |
Yang Y.,Second Military Medical University |
And 2 more authors.
Academic Journal of Second Military Medical University | Year: 2015
Objective To investigate the effect of silk fibroin (SF) on degradation and biocompatibility of poly (L-lactic acid-co-e-caprolactone) (P[LLA-CL]) in vivo. Methods The scaffolds of P(LLA-CL) (w/w=1:1) blended with 25% of SF (SF/P[LLA-CL]) and P(LLA-CL) were prepared by electrospinning. Both kinds of scaffolds were subcutaneously implanted in 45 6-month-old rats for up to 6 months to evaluate their degradation and biocompatibility characteristics. Results Pathological sections showed P(LLA-CL) scaffold become swollen and began to separate into different layers after 3 months, and then become broken after 6 months; while SF/P (LLA-CL) scaffold largely maintained its structure after 6 months. Immunohistochemical staining showed a large number of macrophages on the surface and in P(LLA-CL) scaffolds 1 month after implantation, and they could still be found 3 months after implantation, accompanied by foreign body giant cells; while no obvious macrophages or foreign body giant cells were found in SF/P(LLA-CL) scaffolds at different time points. Examination of inflammatory gene expression showed that TNF-α and IL-10 expression in P(LLA-CL) scaffolds was significantly higher than that in SF/P(LLA-CL) scaffolds 1 week after implantation (P<0. 05), the same was also true for TNF-α, IL-1β and IL-10 expression 1 month after implantation (P<0. 05), for TNF-α and IL-10 expression 2 months after implantation (P<0. 05), for TGF-β expression 3 months after implantation (P<0. 05%, and for IL-1β and TGF-β expression 6 months after implantation (P<0. 05%). Conclusion SF incorporation can delay degradation, reduce inflammation, and improve the biocompatibility of P(LLA-CL) scaffolds, which may provide reference for scaffold design in tissue engineering. © 2015, Academic Journal of Second Military Medical University. All rights reserved. Source
Zhang B.,First Central Hospital of Baoding |
Zhao X.,First Central Hospital of Baoding |
Zhang J.,First Central Hospital of Baoding |
Jia W.,Clinical Laboratory |
And 3 more authors.
Cancer Research and Clinic | Year: 2016
Objective: To investigate the expression and clinical significance of apoptotic protease activating factor 1 (Apaf-1) and bax in prostate cancer (PCa) and benign prostatic hyperplasia (BPH). Methods: Immunohistochemistry was used to detect the expression of Apaf-1 and bax in the tissues from 45 PCa patients and 60 BPH patients. Results: The positive rates of Apaf-1 and bax in PCa tissues were 22.22 % (10/45) and 20.00 % (9/45), respectively, while those in BPH tissues were 48.33 % (29/60) and 46.67 % (28/60). There was a statistically significant difference in the expressions of Apaf-1 and bax between two groups (P < 0.05). The expressions of Apaf-1 and bax were not correlated with the age of patients and distant metastasis (P > 0.05), but they were correlated with the pathological grade and clinical stage of PCa (P < 0.05). The expressions of Apaf-1 and bax in PCa tissues were lower than those in BPH tissues. There was a positive correlation between the expression of Apaf-1 and bax (r = 0.535, P < 0.01). Conclusion: Apaf-1 and bax might be correlated with the carcinogenesis and development of PCa. © Copyright 2016 by the Chinese Medical Association. Source