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Wang S.-L.,First Affiliated Hospital of Yunnan Province | Dan Q.-Q.,University of Sichuan | Rong R.,Cadres Ward | Wang T.-H.,University of Sichuan | Zhang Y.-H.,First Affiliated Hospital of Yunnan Province
Journal of Sichuan University (Medical Science Edition) | Year: 2012

Objective To construct the recombinant vector of human single herpes virus (HSV) carried brain derived neurotrophic factor (BDNF) gene. Methods BDNF gene was acquired from rat brain tissue by RT-PCR, then was cloned into plasmid, and enveloped by HSV. The recombinant was used to transfer cultured cortical neurons. The number and neurite length of neurons were quantified. The BDNF level and subcellular localization were detected by ELISA and immunohistochemistry. Results HSV carried BDNF gene recombinant has been successfully constructed. The recombinant showed the bioactivity on the growth of cortical neurons. BDNF level was increased significantly in BDNF transferred group. Conclusion HSV carried BDNF gene recombinant, with the bioactivity, has been successfully constructed. This could provide the vector for the treatment of BDNF under disease condition base on transferring gene technique.


Jin H.,First Affiliated Hospital of Yunnan Province | Dan Q.-Q.,University of Sichuan | Rong R.,University of Sichuan | Wang T.-H.,University of Sichuan
Journal of Sichuan University (Medical Science Edition) | Year: 2012

Objective To explore the change of brain derived neurotrophic factor (BDNF) expression and its relationship with the neurological behavior after spinal cord transection (SCT) in rats. Methods 66 adult SD rats were assigned randomly to sham operation group and SCT group. Rats in SCT group were subjected T 10 -T11 spinal cord transection and allowed to survived 1 d, 3 d, 7 d, 14 d, 21 d and 28 d. BBB scores in hindlimbs were observed at 0, 7, 14, 21 and 28 days post operation (dpo). The BDNF expression was determined by using ELISA (28 d) and RT-PCR (each time point) techniques. The localization of BDNF and its mRNA at sham and 28 d after SCT was also observed by immunohistochemistry and in situ hybridization. Results After SCT, the motor function in hindlimbs disappear immediately. The BBB scores get a gradual recovery from 14 dpo to 28 dpo, when compared with that of former timepoint (P<0.05). BDNF and its mRNA were located in cytoplasma and neurites. The level of BDNF mRNA (indicated by RT-PCR) was upregulated at 14 days after SCT than that in 1 and 3 dpo (P< 0.05). The contents of BDNF in the injuried spinal cords at 28 dpo were increased than that in the sham operation group (P<0.05). Conclusion Neuroplasticcity has occurred in rats subjected to SCT, and the mechanism may be involved in the increase of BDNF expression in the spinal cord with the time going.


Yuan B.,First Affiliated Hospital of Yunnan Province | Yang R.-A.,First Affiliated Hospital of Yunnan Province | Zhao W.,First Affiliated Hospital of Yunnan Province | Xu Y.-Y.,First Affiliated Hospital of Yunnan Province | And 2 more authors.
Journal of Sichuan University (Medical Science Edition) | Year: 2015

Objective To investigate expressional changes of brain derived neurotrophic factor (BDNF) in the trachea of rats with acrolein inhalation. Methods Twenty two SD rats were divided into 2 groups: the rats in experimental group were subjected to acrolein inhalation for the induce of trachea inflammatory injury, while the rats with saline (NS) inhalation were as control. All the rats were sacrificed in 1, 3, 6 weeks after acrolein (n = 11 at each time point) or saline inhalation (n = 11 at each time point), the samples of trachea epithelium were harvested. The immunohistochemistry and in situ hybridization was performed to detect the location of BDNF protein and mRNA in trachea. The expression of BDNF mRNA in the trachea tissues were determined by RT-PCR. Results There are positive cells in epithelium of trachea for BDNF protein and mRNA, with cytoplasm staining. The expression of BDNF mRNA in the trachea was increased at 1 week after acrolein inhalation (P<0.05, vs. control group), then decreased along with the time and reached to the same level as control group at 3 weeks, then last to 6 weeks (P> 0.05). Conclusion The inflammatory injury in trachea induced by acrolein exposure could be associated with the increased expression of BDNF. BDNF may be one of the crucial inflammatory factors in the process of inflammatory reaction in trachea with acrolein stimulation.

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