the First Affiliated Hospital of Yunnan Province

Kunming, China

the First Affiliated Hospital of Yunnan Province

Kunming, China
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PubMed | the First Affiliated Hospital of Yunnan Province
Type: Journal Article | Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition | Year: 2011

To investigate the changes of angiotensin II (Ang II) expression in a rat model of mucus hypersecretion induced by acrolein.A rat model of mucous hypersecretion of airway was established by acrolein-inhalation. The AB/PAS stain was performed to evaluate the mucous secretion level. Immunohistochemistry and Western blot were employed to detect the expression of Ang II protein.Hypersecretion of mucus occurred at 3 weeks and peaked at 6 weeks post exposure (wpe) to acrolein. Increased immunostaining and protein level of Ang II were readily detected in rat lung at 3 wpe and enhanced at 6 wpe.The increase of Ang II expression, which associated to pulmonary mucus hypersecretion, might play a role in the lung injury induced by acrolein exposure.


PubMed | the First Affiliated Hospital of Yunnan Province
Type: Journal Article | Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition | Year: 2011

To explore the changes of Muc5ac expression in a rat model of mucus hypersecretion induced by acrolein.The rat model of mucus hypersceretion was established by acrolein exposure. RT-PCR, ISH, immunohistochemistry and Western blot were employed to detect the mRNA and protein levels of Muc5ac in the rats airway.The expression of Muc5ac was detected in trachea and lung at 3 weeks post exposure (wpe) and the expression level was enhanced at 6 wpe. ISH confirmed that Muc5ac was synthesized endogenously.The pulmonary mucus hypersecretion is associated to the increase of intrinsic Muc5ac expression in the rat airway exposure to acrolein.


PubMed | the First Affiliated Hospital of Yunnan Province
Type: Journal Article | Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition | Year: 2011

To investigate the effects of enalapril on nuclear factor-kappa B (NF-kappaB) expression in a rat model of mucus hypersecretion induced by acrolein.The rat model of mucus hypersecretion was established by the inhalation of acrolein. Then the mucus hypersecretion rats were treated by enalapril. The AB/PAS stain was performed to measure the secretion of airway in the rats, while the mRNA and protein expression levels of NF-kappaB in the airway were detected by RT-PCR, ISH, immunohistochemistry and Western blot.With the inhalation of acrolein, the airway secretion increased obviously, while the mRNA and protein expressions of NF-kappaB in lung were increased. The treatment of enalapril resulted in a significant decrease of airway secretion, as well as the downregulation of NF-kappaB in both protein and gene level.Enalapril (ACE inhibitor) treatment could effectively decrease pulmonary mucus hypersecretion in acrolein-treated rats, which may be related to the downregulation of NF-kappaB expression.


PubMed | the First Affiliated Hospital of Yunnan Province
Type: Journal Article | Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition | Year: 2011

To investigate the effects of enalapril on the expressions of IL-1beta and IL-6 in the lung of rats treated with acrolein inhalation.Inflammatory lung injury was induced by acrolein inhalation in rats. The rats were divided into natrium solution (NS) group, acrolein group, enalapril and acroclein (EA) group, enalapril and NS (EN) group. Lungs were harvested from the rats in each group at 1 week, 3 weeks, 6 weeks after the treatment of natrium solution, acrolein inhalation and/or enalapril. The mRNA and protein expressions of IL-1beta and IL-6 in the lung tissues were measured by RT-PCR, in situ hybridization, immunohistochemistry and Western blot.Increased immunostaining, protein level and mRNA expression of IL-1beta and IL-6 were found in rat lung at 3 weeks and reached to the peak at 6 weeks post exposure to acrolein. The administration of enalapril resulted in a significant downregulation of IL-1beta and IL-6 in both protein and gene level, accompany with the decrease of inflammation.Enalapril, as a ACE inhibitor, could protect the airway from inflammation injury in acrolein-treated rats via the down-regulation of IL-1beta and IL-6 expression.


PubMed | the First Affiliated Hospital of Yunnan Province
Type: Journal Article | Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition | Year: 2012

To explore the change of brain derived neurotrophic factor (BDNF) expression and its relationship with the neurological behavior after spinal cord transection (SCT) in rats.66 adult SD rats were assigned randomly to sham operation group and SCT group. Rats in SCT group were subjected T10-T11 spinal cord transection and allowed to survived 1 d, 3 d, 7 d, 14 d, 21 d and 28 d. BBB scores in hindlimbs were observed at 0, 7, 14, 21 and 28 days post operation (dpo). The BDNF expression was determined by using ELISA (28 d) and RT-PCR (each time point) techniques. The localization of BDNF and its mRNA at sham and 28 d after SCT was also observed by immunohistochemistry and in situ hybridization.After SCT, the motor function in hindlimbs disappear immediately. The BBB scores get a gradual recovery from 14 dpo to 28 dpo, when compared with that of former timepoint (P < 0.05). BDNF and its mRNA were located in cytoplasma and neurites. The level of BDNF mRNA (indicated by RT-PCR) was upregulated at 14 days after SCT than that in 1 and 3 dpo (P< 0. 05). The contents of BDNF in the injuried spinal cords at 28 dpo were increased than that in the sham operation group (P < 0.05).Neuroplasticity has occurred in rats subjected to SCT, and the mechanism may be involved in the increase of BDNF expression in the spinal cord with the time going.


PubMed | the First Affiliated Hospital of Yunnan Province
Type: Journal Article | Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition | Year: 2012

To construct the recombinant vector of human single herpes virus (HSV) carried brain derived neurotrophic factor (BDNF) gene.BDNF gene was acquired from rat brain tissue by RT-PCR, then was cloned into plasmid, and enveloped by HSV. The recombinant was used to transfer cultured cortical neurons. The number and neurite length of neurons were quantified. The BDNF level and subcellular localization were detected by ELISA and immunohistochemistry.HSV carried BDNF gene recombinant has been successfully constructed. The recombinant showed the bioactivity on the growth of cortical neurons. BDNF level was increased significantly in BDNF transferred group.HSV carried BDNF gene recombinant, with the bioactivity, has been successfully constructed. This could provide the vector for the treatment of BDNF under disease condition base on transferring gene technique.

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