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Qu P.,PLA Fourth Military Medical University | Huang X.,PLA Fourth Military Medical University | Zhou X.,PLA Fourth Military Medical University | Lu Z.,PLA Fourth Military Medical University | And 5 more authors.
Histopathology | Year: 2015

Aims: CD155 is an important ligand in triggering tumour rejection by immune cells. However, the expression of CD155 and its clinical significance in hepatocellular carcinoma (HCC) remains unknown. Methods and results: We examined the expression level of CD155 in 174 HCC tissue samples by immunohistochemical staining and in HCC cell lines by flow cytometry; 63.8% (111 of 174) of HCC tissue samples showed negative CD155 expression. When compared with adjacent peritumour tissues, HCC tissues exhibited a significantly lower expression of CD155 (P < 0.001). Flow cytometry analysis indicated that HCC cell lines had low levels of CD155 expression. Moreover, negative CD155 expression was associated significantly with higher serum α-fetoprotein level (P = 0.016) and a higher incidence of portal vein tumour thrombus (P = 0.050). Importantly, patients with positive CD155 expression had better overall survival after surgery than those with negative CD155 expression (P = 0.005). Furthermore, Cox regression analyses showed that CD155 expression was an independent prognostic factor for HCC (P = 0.049). Conclusions: Our findings suggest that loss of CD155 expression may play an important role in the immune escape of HCC cells and thus CD155 may serve as a prognostic marker as well as a potential therapeutic target for HCC. © 2014 John Wiley & Sons Ltd. Source


Li Z.-C.,The First Affiliated Hospital of the General Hospital of PLA | Zhang J.,Chinese PLA General Hospital | Hu D.-M.,The First Affiliated Hospital of the General Hospital of PLA | Wang Q.,The First Affiliated Hospital of the General Hospital of PLA | And 2 more authors.
Zhonghua nan ke xue = National journal of andrology | Year: 2014

OBJECTIVE: To investigate the effect of transrectal ultrasound-guided microwave ablation of canine prostate tissue.METHODS: Guided by transrectal ultrasound, we conducted microwave ablation on each side of the prostate in 12 male dogs, 6 at 40 W/ 120 s (group A) and the other 6 at 40 W/160 s (group B), and observed the changes in the thermal lesions using grayscale ultrasound. After thermal ablation, we measured the volume of the thermal lesions by contrast-enhanced ultrasound (CEUS). Then we harvested the whole prostate from the animals and determined the lesion volumes in the fresh tissue specimens.RESULTS: Grayscale ultrasound revealed an echogenic area at the initiation of the microwave ablation procedure, which was enlarged with the increase of ablation time. At the end of the procedure, the lesions appeared as an irregular heterogeneous echogenic area. CEUS showed oval non-perfused areas, which appeared as well-defined non-echoic areas in sharp contrast with the surrounding normal prostate parenchyma with bolus injection of contrast material (Sonovue, 2.4 ml), and that the thermal lesion volumes of groups A and B were (1.18 +/- 0.23) cm3 and (1.52 +/- 0.23) cm3, respectively. The thermal lesions of the gross specimen exhibited an elliptical shape, pale color and clear margin, and their volumes were (1.13 +/- 0.20) cm3 and (1.48 +/- 0.20) cm3, respectively, in groups A and B.CONCLUSION: Different combinations of time and power can produce coagulative necrotic lesions of different volumes in the local prostatic tissue. CEUS can accurately manifest the lesion area and thus avoid excessive or inadequate ablation treatment. Source


Xia H.,The First Affiliated Hospital of the General Hospital of PLA | Zhang Y.-M.,The First Affiliated Hospital of the General Hospital of PLA | Yu C.-H.,The First Affiliated Hospital of the General Hospital of PLA | Zhang W.,The First Affiliated Hospital of the General Hospital of PLA | And 2 more authors.
National Medical Journal of China | Year: 2012

Objective: To define the role of interferon-γ on radiotherapy of lung cancer and explore a new way to clinical treatment. Methods: A549 cells were exposed to γ ray with or without IFN-γ co-treatment. MTT assay was performed to evaluate cell viability. Western blot was used to observe the expression of P53 protein. Results The results showed that co-treatment of IFN-γ decreased the cell viability significantly compared with the γ ray irradiation group(71.4%±2.1% vs 44.1%±3.1%, n=7, P<0.01). In addition, the expression of P53 protein also increased significantly after co-treatment (P<0.01); Furthermore, the cell cycle was changed obviously in co-treatment group compared with γ ray irradiation group, S phase increased (12.9% vs 209%, n=5, P<0.05) and also blocked the G2/M phase (28.8% vs 38.9%, n=5, P<0.05). Conclusions: The results suggested that γ ray irradiation combined with IFN-γ can increase the efficiency of radiotherapy on A549 cells and there is much broad prospect in the clinical treatment of lung cancer. Source


Xia H.,The First Affiliated Hospital of the General Hospital of PLA | Ma Y.-F.,The First Affiliated Hospital of the General Hospital of PLA | Yu C.-H.,The First Affiliated Hospital of the General Hospital of PLA | Li Y.-J.,The First Affiliated Hospital of the General Hospital of PLA | And 4 more authors.
Experimental Physiology | Year: 2014

Non-small cell lung cancer (NSCLC) is one of the most common diseases encountered in medical oncology practice. The aim of the present study was to test the antitumour effects of short-hairpin RNA targeting aquaporin 3 (AQP3) in experimental NSCLC. Expression of AQP3 was suppressed in human A549 and H1299 NSCLC cell lines by short-hairpin RNA-mediated silencing. Therapeutic effects were assessed by examining tumorigenicity using a subcutaneous xenograft mouse model of NSCLC. Aquaporin 3 knockdown inhibited tumour growth and prolonged survival of mice with tumours. Aquaporin 3 knockdown suppressed tumour proliferation, marked by enhanced expression of p53, an increased ratio of cleaved caspase 3 to pro-caspase 3 and reduced expression of proliferating cell nuclear antigen and B-cell lymphoma-2 (bcl-2). Aquaporin 3 knockdown inhibited tumour angiogenesis, marked by decreased CD31 immunostaining and reduced expression of hypoxia-inducible factor-2α and vascular endothelial growth factor. Aquaporin 3 knockdown reduced cellular glycerol content and suppressed mitochondrial ATP formation. Aquaporin 3 knockdown in vitro significantly suppressed activities of matrix metalloproteinases MMP2 and MMP9, reduced AKT phosphorylation and decreased cell invasiveness of A549 and H1299 cells. In conclusion, AQP3 knockdown suppressed tumour growth and reduced angiogenesis in human NSCLS xenografts. Aquaporin 3 could thus be envisaged as a novel therapeutic target for NSCLC. © 2014 The Physiological Society. Source

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