The First Affiliated Hospital of the General Hospital of PLA

Beijing, China

The First Affiliated Hospital of the General Hospital of PLA

Beijing, China
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Qu P.,PLA Fourth Military Medical University | Huang X.,PLA Fourth Military Medical University | Zhou X.,PLA Fourth Military Medical University | Lu Z.,PLA Fourth Military Medical University | And 5 more authors.
Histopathology | Year: 2015

Aims: CD155 is an important ligand in triggering tumour rejection by immune cells. However, the expression of CD155 and its clinical significance in hepatocellular carcinoma (HCC) remains unknown. Methods and results: We examined the expression level of CD155 in 174 HCC tissue samples by immunohistochemical staining and in HCC cell lines by flow cytometry; 63.8% (111 of 174) of HCC tissue samples showed negative CD155 expression. When compared with adjacent peritumour tissues, HCC tissues exhibited a significantly lower expression of CD155 (P < 0.001). Flow cytometry analysis indicated that HCC cell lines had low levels of CD155 expression. Moreover, negative CD155 expression was associated significantly with higher serum α-fetoprotein level (P = 0.016) and a higher incidence of portal vein tumour thrombus (P = 0.050). Importantly, patients with positive CD155 expression had better overall survival after surgery than those with negative CD155 expression (P = 0.005). Furthermore, Cox regression analyses showed that CD155 expression was an independent prognostic factor for HCC (P = 0.049). Conclusions: Our findings suggest that loss of CD155 expression may play an important role in the immune escape of HCC cells and thus CD155 may serve as a prognostic marker as well as a potential therapeutic target for HCC. © 2014 John Wiley & Sons Ltd.


Tang J.,The First Affiliated Hospital of The General Hospital of PLA | Liu Y.,Air Force General Hospital | Cao Z.,The First Affiliated Hospital of The General Hospital of PLA | Hu Y.,The First Affiliated Hospital of The General Hospital of PLA | And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2014

This study aims to evaluate clinical efficacy of short segment pedicle screw fixation without bone fusion for unstable thoracolumbar burst fracture. Nineteen patients younger than 40 years old with unstable thoracolumbar burst fractures were included. The surgical procedure included postural reduction for 3 days and screw fixations at one level above, one level below and at the fractured level itself. The implants were removed 12 months after initial operation. Imaging and clinical findings were analyzed at preoperative, 12 months after surgery, just before implant removal, and at six months after implant removal. Results indicated that difference was statistically significant between preoperative period or postoperative 1 year follow-up, just before implant removal and 6 months after implant removal (P < 0.05). Results at postoperative 1 year follow-up, just before implant removal and 6 months after implant removal were better than preoperative period. There were no significant complications or neurological deterioration after screws insert and removal in any patient. The rate of clinical outcome with excellent and good was 94.7%. In conclusion, short segment pedicle screw fixation without bone fusion can be an effective and safe operative srategytechnique in the management of young patients suffering from unstable burst fracture. © 2014, E-Century Publishing Corporation. All rights reserved.


Lu X.,The First Affiliated Hospital of the General Hospital of PLA | Lin B.,The First Affiliated Hospital of the General Hospital of PLA | Tang J.G.,The First Affiliated Hospital of the General Hospital of PLA | Cao Z.,The First Affiliated Hospital of the General Hospital of PLA | Hu Y.,The First Affiliated Hospital of the General Hospital of PLA
African journal of traditional, complementary, and alternative medicines : AJTCAM / African Networks on Ethnomedicines | Year: 2014

Ku Dou Zi is the dried whole plant, roots and seeds of Sophora alopecuroides L. in the genus Sophora of family Leguminosae. The entire plant is bitter in taste, cold in nature, and has the heat clearing, detoxifying, pathogenic wind dispelling dampness, analgesic, and insecticidal effects. Modern pharmacological studies have proved that TASA has pharmacological activities of anti-cancer. The objective of this paper was to investigate the inhibitory effect of total alkaloids of Sophora alopecuroides (TASA), on osteosarcoma cell growth and its mechanism. MTT assay and flow cytometry were used to study the inhibitory effect of TASA on human osteosarcoma cell line OS732. The results showed that the inhibition rates of different concentrations of TASA (1.5, 3, and 4.5g/kg), against human osteosarcoma cell line OS732, were: 18.4%, 27.4% and 52.8%, respectively. TASA has an inhibitory effect on osteosarcoma cell growth.


Xia H.,The First Affiliated Hospital of the General Hospital of PLA | Ma Y.-F.,The First Affiliated Hospital of the General Hospital of PLA | Yu C.-H.,The First Affiliated Hospital of the General Hospital of PLA | Li Y.-J.,The First Affiliated Hospital of the General Hospital of PLA | And 4 more authors.
Experimental Physiology | Year: 2014

Non-small cell lung cancer (NSCLC) is one of the most common diseases encountered in medical oncology practice. The aim of the present study was to test the antitumour effects of short-hairpin RNA targeting aquaporin 3 (AQP3) in experimental NSCLC. Expression of AQP3 was suppressed in human A549 and H1299 NSCLC cell lines by short-hairpin RNA-mediated silencing. Therapeutic effects were assessed by examining tumorigenicity using a subcutaneous xenograft mouse model of NSCLC. Aquaporin 3 knockdown inhibited tumour growth and prolonged survival of mice with tumours. Aquaporin 3 knockdown suppressed tumour proliferation, marked by enhanced expression of p53, an increased ratio of cleaved caspase 3 to pro-caspase 3 and reduced expression of proliferating cell nuclear antigen and B-cell lymphoma-2 (bcl-2). Aquaporin 3 knockdown inhibited tumour angiogenesis, marked by decreased CD31 immunostaining and reduced expression of hypoxia-inducible factor-2α and vascular endothelial growth factor. Aquaporin 3 knockdown reduced cellular glycerol content and suppressed mitochondrial ATP formation. Aquaporin 3 knockdown in vitro significantly suppressed activities of matrix metalloproteinases MMP2 and MMP9, reduced AKT phosphorylation and decreased cell invasiveness of A549 and H1299 cells. In conclusion, AQP3 knockdown suppressed tumour growth and reduced angiogenesis in human NSCLS xenografts. Aquaporin 3 could thus be envisaged as a novel therapeutic target for NSCLC. © 2014 The Physiological Society.


Yin L.,the First Affiliated Hospital of The General Hospital of PLA | Pi Y.,the First Affiliated Hospital of The General Hospital of PLA
Yan ke xue bao = Eye science / "Yan ke xue bao" bian ji bu | Year: 2010

PURPOSE: To study the curative effect of amnion membrane transplantation on decreasing corneal neovascularization(CNV) induced by alkali burn.METHODS: It was a non-randomized retrospective case-control study. Among 19 cases (23 eyes) of third-degree alkali burns from 2006 to 2010, 11 cases (13 eyes) were performed with amnion membrane transplantation operation, and others were not. Amnion membrane transplantation was performed at 3rd day after burns in the treatment group. Ages and treatments beyond surgery of double groups were matched. Areas of CNV in double groups were measured at the 14th and 60th days after burn. RESULTS:Area of CNV in the treatment group was (62.133±8.571) mm² at the 14th day after burn, and was 30.6% lower than that in the control group. Area of CNV in the treatment group was (112.019±17.362)mm² at the 14th day after burn, and was 13.5% lower than that in the control group. There was statistical significance between the two groups (P<0.05).CONCLUSION: Amnion membrane transplantation operation can inhibit the growth of corneal neovascularization induced by alkali burn.


PubMed | the First Affiliated Hospital of the General Hospital of PLA
Type: Journal Article | Journal: Chinese journal of integrative medicine | Year: 2012

To study the effect of Vaccinium uliginosum L., (VU) on the electroretinogram (ERG) and retinal pathological changes in rabbits after light-induced damage.Twenty-eight Chinchilla rabbits were randomly divided into four groups: administration beforehand (A), administration after injury (B), light injury without administration (C), and blank (D) groups. After a 4-week administration of VU homogenate at 4.8 g/(kgd) once a day in group A, ERG in groups A, B and C were recorded according to the standards set by the International Society for Clinical Electrophysiology of Vision (ISCEV). Except for group D, the groups were then exposed to strong light. Just after that, group A stopped receiving VU treatment and group B started to receive it. Then ERGs in all groups were recorded after 1 day, 1 week, and 2 weeks. Throughout the whole process groups which were not fed with VU were fed with normal saline. Finally, the tissues and structures of all the groups were observed and the thickness of the outer nuclear layers (ONL) was measured.(1) After 4-week feeding with VU, the latency time of ERG in group A became shorter than those in the other groups and the amplitude increased. After being exposed to strong light, the latency time lengthened and amplitude decreased in all the injury groups, but comparing at each time point, the measured values in group A were better than those in group C. With the accumulation of VU, the ERG in group B improved, and finally, all of the detected values became better than those in group C. (2) Retinae in group D were normal in histology and the layers were in order but those in group C became disarranged. The injuries in groups A and B were minor compared with those in group C. The thickness of the ONL in group C was significantly thinner than in the other groups (P=0.000), and that in groups A and B was thicker than that in group C, although thinner than in group D. That in group A was thicker than in group B.VU can relieve the injury to rabbit retinae exposed to normal day and night rhythm, alleviate the harm caused by light when used beforehand, and repair the light damage to the retina.


PubMed | the First Affiliated Hospital of the General Hospital of PLA
Type: Journal Article | Journal: Acta pharmacologica Sinica | Year: 2012

Sirtuin 1 (Sirt1) is the class III histone/protein deacetylase that interferes with the NF-B signaling pathway, thereby has anti-inflammatory function. This study was undertaken to investigate whether Sirt1 could protect osteoblasts against TNF--induced injury in vitro.Murine osteoblastic cell line, MC3T3-E1, was used. Overexpress of Sirt1 protein in MC3T3-E1 cells was made by transfection the cells with Sirt1-overexpressing adenovirus. The levels of mRNAs and proteins were determined with qRT-PCR and Western blotting, respectively. The activity of NF-B was examined using NF-B luciferase assay. The NO concentration was measured using the Griess method.Treatment of MC3T3-E1 cells with TNF- (2.5-10 ng/mL) suppressed Sirt1 protein expression in a concentration-dependent manner. TNF- (5 ng/mL) resulted in an increase in apoptosis and a reduction in ALP activity in the cells. Overexpression of Sirt1 in the cells significantly attenuated TNF--induced injury through suppressing apoptosis, increasing ALP activity, and increasing the expression of Runx2 and osteocalcin mRNAs. Furthermore, overexpression of Sirt1 in the cells significantly suppressed TNF--induced NF-B activation, followed by reducing the expression of iNOS and NO formation. Sirt1 activator resveratrol (10 mol/L) mimicked the protection of the cells by Sirt1 overexpression against TNF--induced injury, which was reversed by the Sirt1 inhibitor EX-527 (5 mol/L).Overexpression of Sirt1 protects MC3T3-E1 osteoblasts aganst TNF--induced cell injury in vitro, at least in part, via suppressing NF-B signaling. Sirt1 may be a novel therapeutic target for treating rheumatoid arthritis-related bone loss.


PubMed | The First Affiliated Hospital of the General Hospital of PLA and The General Hospital of PLA
Type: | Journal: European journal of pharmacology | Year: 2016

Metabotropic glutamate receptor 1 (mGlu1 receptor) is expressed in many cancer cell types as compared to normal counterparts underscoring its potential role in tumor behavior. The aim of present study was to test the role of mGlu1 receptor in experimental non-small cell lung cancer (NSCLC). First, protein expression of mGlu1 receptor was higher in human NSCLC cell lines, including both adenocarcinoma and squamous carcinoma subtypes, when compared to normal bronchial epithelial cells. Inhibition of mGlu1 receptor by BAY36-7620 (an mGlu1 receptor-specific inhibitor) inhibited tumor growth and prolonged survival of mice with tumors of A549 or H1299. Treatment with BAY36-7620 suppressed AKT phosphorylation in A549 tumors and pre-treatment with BAY36-7620 blocked the L-quisqualate (a potent mGlu1 receptor agonist)-induced AKT phosphorylation in A549 cells. Treatment with BAY36-7620 reduced cellular proliferation of A549 cells. Treatment with BAY36-7620 enhanced cleaved PARP levels and reduced protein expression of bcl-2, HIF-1, and VEGF. In contrast, treatment with L-quisqualate reduced cleaved PARP levels and enhanced protein expression of bcl-2, HIF-1, VEGF, and IL-8, which was reversed by co-incubation with MK2206 (an AKT inhibitor). Pre-treatment with BAY36-7620 blocked the VEGF-induced AKT phosphorylation in HUVECs. Treatment of HUVECs with L-quisqualate resulted in enhancement of capillary tube formation, which was reversed by co-incubation with MK2206. Furthermore, mGlu1 receptor knockdown suppressed tumor growth and prolonged survival of mice with tumors of A549 or H1299. Collectively, inhibition of mGlu1 receptor suppressed tumor growth and angiogenesis in experimental NSCLC.


PubMed | the First Affiliated Hospital of the General Hospital of PLA
Type: Journal Article | Journal: Zhonghua yi xue za zhi | Year: 2012

To define the role of interferon- on radiotherapy of lung cancer and explore a new way to clinical treatment.A549 cells were exposed to ray with or without IFN- co-treatment. MTT assay was performed to evaluate cell viability. Western blot was used to observe the expression of P53 protein.The results showed that co-treatment of IFN- decreased the cell viability significantly compared with the ray irradiation group (71.4% 2.1% vs 44.1% 3.1%, n = 7, P < 0.01). In addition, the expression of P53 protein also increased significantly after co-treatment (P < 0.01); Furthermore, the cell cycle was changed obviously in co-treatment group compared with ray irradiation group, S phase increased (12.9% vs 20.9%, n = 5, P < 0.05) and also blocked the G2/M phase (28.8% vs 38.9%, n = 5, P < 0.05).The results suggested that ray irradiation combined with IFN- can increase the efficiency of radiotherapy on A549 cells and there is much broad prospect in the clinical treatment of lung cancer.


PubMed | The First Affiliated Hospital of the General Hospital of PLA and The General Hospital of PLA
Type: Journal Article | Journal: Zhonghua nan ke xue = National journal of andrology | Year: 2014

To investigate the effect of transrectal ultrasound-guided microwave ablation of canine prostate tissue.Guided by transrectal ultrasound, we conducted microwave ablation on each side of the prostate in 12 male dogs, 6 at 40 W/ 120 s (group A) and the other 6 at 40 W/160 s (group B), and observed the changes in the thermal lesions using grayscale ultrasound. After thermal ablation, we measured the volume of the thermal lesions by contrast-enhanced ultrasound (CEUS). Then we harvested the whole prostate from the animals and determined the lesion volumes in the fresh tissue specimens.Grayscale ultrasound revealed an echogenic area at the initiation of the microwave ablation procedure, which was enlarged with the increase of ablation time. At the end of the procedure, the lesions appeared as an irregular heterogeneous echogenic area. CEUS showed oval non-perfused areas, which appeared as well-defined non-echoic areas in sharp contrast with the surrounding normal prostate parenchyma with bolus injection of contrast material (Sonovue, 2.4 ml), and that the thermal lesion volumes of groups A and B were (1.18 +/- 0.23) cm3 and (1.52 +/- 0.23) cm3, respectively. The thermal lesions of the gross specimen exhibited an elliptical shape, pale color and clear margin, and their volumes were (1.13 +/- 0.20) cm3 and (1.48 +/- 0.20) cm3, respectively, in groups A and B.Different combinations of time and power can produce coagulative necrotic lesions of different volumes in the local prostatic tissue. CEUS can accurately manifest the lesion area and thus avoid excessive or inadequate ablation treatment.

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