The First Affiliated Hospital of the General Hospital of CPLA

Beijing, China

The First Affiliated Hospital of the General Hospital of CPLA

Beijing, China
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PubMed | The First Affiliated Hospital of the General Hospital of CPLA and The General Hospital of CPLA
Type: Journal Article | Journal: Biochemical and biophysical research communications | Year: 2014

Forkhead transcription factors are essential for diverse processes in early embryonic development and organogenesis. As a member of the forkhead family, FOXD1 is required during kidney development and its inactivation results in failure of nephron progenitor cells. However, the role of FOXD1 in carcinogenesis and progression is still limited. Here, we reported that FOXD1 is a potential oncogene in breast cancer. We found that FOXD1 is up-regulated in breast cancer tissues. Depletion of FOXD1 expression decreases the ability of cell proliferation and chemoresistance in MDA-MB-231 cells, whereas overexpression of FOXD1 increases the ability of cell proliferation and chemoresistance in MCF-7 cells. Furthermore, we observed that FOXD1 induces G1 to S phase transition by targeting p27 expression. Our results suggest that FOXD1 may be a potential therapy target for patients with breast cancer.


Ge C.,The 307th Hospital of PLA | Sun H.,The 307th Hospital of PLA | Li Q.,The 307th Hospital of PLA | Lu R.,The 307th Hospital of PLA | And 5 more authors.
Journal of Biomaterials and Tissue Engineering | Year: 2016

Zoledronic acid (ZOL) is the third generation nitrogen containing bisphosphonate widely used for the treatment of cancer-induced bone diseases. Prior studies showed that ZOL reduced the number of endothelial cells and subsequently inhibited angiogenesis after tooth extraction, leading to bisphosphonates-induced osteonecrosis of the jaw (BP-ONJ). However, its underlying molecular mechanisms are still unclear. Our results showed that ZOL concentration-dependently inhibited cell viability, migration, adhesion and tube formation by decreasing vascular endothelial growth factor (VEGF) expression and secretion. In addition, ZOL decreased HIF-1α protein level, but had no effect on HIF-1α mRNA level and promoter activity. Mechanically, we found that ZOL attenuated HIF-1α protein stability through attenuating the activation of PI3K/AKT/mTOR and MAP kinase pathways. Moreover, ZOL impaired HIF-1α/Hsp90 and HIF-1α/p300 interactions, which are responsible for HIF-1α destabilization. Overexpression of Hsp90 or p300 with adenovirus significantly inhibited ZOL-induced the decrease of HIF-1α and VEGF protein expression. Collectively, our data demonstrate that ZOL exhibits an antiangiogenic effect via inhibition of HIF-1α-dependent VEGF expression and secretion, which is due to destabilization of HIF-1α protein. © 2016 American Scientific Publishers.


Sun W.,the First Affiliated Hospital of the General Hospital of CPLA | Wang H.,the First Affiliated Hospital of the General Hospital of CPLA | Wen Z.,the First Affiliated Hospital of the General Hospital of CPLA | Ma N.,the First Affiliated Hospital of the General Hospital of CPLA | And 4 more authors.
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases | Year: 2016

CONCLUSIONS: The common clinical manifestations in patients with both LC and PE included unexplained dyspnea, fever and cough. The most common pathological type was adenocarcinoma. The first 5 months after LC diagnosis were the peak time for PE. Patients with LC and PE had a shorter survival time. LC of grade III to IV, lower Hb, higher WBC, higher D-Dimer and hyoxemia were independent risk factors for LC complicated with PE.OBJECTIVE: To investigate the clinical characteristics, risk factors, and survival time of patients with lung cancer (LC) and pulmonary embolism (PE).METHODS: A total of 17 LC patients complicated with PE admitted to this hospital from February 2012 to January 2014 were retrospectively reviewed. There were 13 males and 4 females, with an average age of (65±9) years (range, 38-82 years). Twenty LC patients, including 14 males and 6 females with an average age of (63±9) years (range, 34-81 years), and 10 PE patients , including 7 males and 3 females with an average age of (70±7) years (range, 42-85 years), were selected respectively as the LC control group and the PE control group. Logistic regression analysis was used to evaluate the risk factors for LC complicated with PE. The survival of these patients was compared with that of the control subjects by Kaplan-Meier analysis.RESULTS: In the 17 patients with LC and PE, the diagnosis of PE was made simultaneously with LC in 2, before the diagnosis of LC in 4, and after the diagnosis of LC in 11 patients. These patients showed a higher incidence of unexplained dyspnea (12 cases) than those with LC only (6 cases) (P<0.05). These patients also had a higher incidence of cough (11 cases) than those with PE only (2 cases) (P<0.05). The patients with both PE and LC had a lower PaO2 (67±18) mmHg (1 mmHg=0.133 kPa) than those with LC only (87±12) mmHg (P<0.05). They also showed higher WBC count (8.9±5.3) g/L and D-Dimer level (850±537) μg/L than those with LC only (4.5±3.0) g/L, (306±188) μg/L (P<0.05). Multi-factor analysis showed that Hb<100 g/L, WBC>11×10(9)/L, D-Dimer>500 μg/L, PO2<80 mmHg, adenocarcinoma, and high pathological grade (TNM grade) were the risk factors for LC with PE (odds ratio 1.58, 2.24, 3.06, 3.15, 3.44, 2.09, respectively). On January 31, 2014, the median survival time of patients with LC and PE was 8.7 months, which was significantly lower than that of patients with LC only (P<0.05).


PubMed | the First Affiliated Hospital of the General Hospital of CPLA
Type: Journal Article | Journal: Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases | Year: 2016

To investigate the clinical characteristics, risk factors, and survival time of patients with lung cancer (LC) and pulmonary embolism (PE).A total of 17 LC patients complicated with PE admitted to this hospital from February 2012 to January 2014 were retrospectively reviewed. There were 13 males and 4 females, with an average age of (659) years (range, 38-82 years). Twenty LC patients, including 14 males and 6 females with an average age of (639) years (range, 34-81 years), and 10 PE patients , including 7 males and 3 females with an average age of (707) years (range, 42-85 years), were selected respectively as the LC control group and the PE control group. Logistic regression analysis was used to evaluate the risk factors for LC complicated with PE. The survival of these patients was compared with that of the control subjects by Kaplan-Meier analysis.In the 17 patients with LC and PE, the diagnosis of PE was made simultaneously with LC in 2, before the diagnosis of LC in 4, and after the diagnosis of LC in 11 patients. These patients showed a higher incidence of unexplained dyspnea (12 cases) than those with LC only (6 cases) (P<0.05). These patients also had a higher incidence of cough (11 cases) than those with PE only (2 cases) (P<0.05). The patients with both PE and LC had a lower PaO2 (6718) mmHg (1 mmHg=0.133 kPa) than those with LC only (8712) mmHg (P<0.05). They also showed higher WBC count (8.95.3) g/L and D-Dimer level (850537) g/L than those with LC only (4.53.0) g/L, (306188) g/L (P<0.05). Multi-factor analysis showed that Hb<100 g/L, WBC>1110(9)/L, D-Dimer>500 g/L, PO2<80 mmHg, adenocarcinoma, and high pathological grade (TNM grade) were the risk factors for LC with PE (odds ratio 1.58, 2.24, 3.06, 3.15, 3.44, 2.09, respectively). On January 31, 2014, the median survival time of patients with LC and PE was 8.7 months, which was significantly lower than that of patients with LC only (P<0.05).The common clinical manifestations in patients with both LC and PE included unexplained dyspnea, fever and cough. The most common pathological type was adenocarcinoma. The first 5 months after LC diagnosis were the peak time for PE. Patients with LC and PE had a shorter survival time. LC of grade III to IV, lower Hb, higher WBC, higher D-Dimer and hyoxemia were independent risk factors for LC complicated with PE.

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