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Wang H.W.,First Affiliated Hospital of General Hospital of Peoples Liberation Army
Chinese medical journal

Intralobar sequestration (ILS) is an uncommon abnormality that accounts for 75% of all pulmonary sequestrations. Over the years there have been several reports of various presenting signs of which hemoptysis was commonly described, however, massive hemoptysis and hemothorax is extremely rare in literature. We present a case of a 45-year-old man who died of fatal complication from an ILS. This case report shows an uncommon presentation of ILS with massive hemoptysis and hemothorax resulting in a dramatic course of disease and a fatal outcome, and for this reason in the absence of trauma or other causes for massive hemoptysis, hemothorax, or lung hematoma, this possibility should be kept in mind so as to avoid misdiagnosis, and resection of the sequestered tissue should be considered in all patients. Source

Tang W.-Q.,First Affiliated Hospital of General Hospital of Peoples Liberation Army | Hou B.-K.,General Hospital of Peoples Liberation Army
International Journal of Ophthalmology

AIM: To investigate the role of heparanase-1 in laser-induced choroidal neovascularization (CNV). METHODS: Experimental CNV was induced by krypton laser photocoagulation in 15 male Brown Norway rats. Fundus fluorescein angiography and histopathological examination were performed in observing the CNV development. The expression and distribution of heparanase-1 protein in the laser lesions were determined by immunohistochemistry and western blotting analysis. RESULTS: The success rate of laser induced CNV was approximately 75% on 3-4 weeks after laser photocoagulation. The protein levels of heparanase-1 increased significantly in the retina-choroidal complex of CNV models when compared to normal rat eyes (P<0.01). Immunostaining confirmed strong heparanase-1 expressions in all laser lesions, and it display ed to be highest at the newly form ed blood vessels within the fibrovascular complex in the subretinal space. CONCLUSION: Heparanase-1 is closely involved in the development of laser induced CNV. Copyright International Journal of Ophthalmology Press. Source

Wang H.-W.,First Affiliated Hospital of General Hospital of Peoples Liberation Army | Lu J.-Y.,First Affiliated Hospital of General Hospital of Peoples Liberation Army | Wang L.,General Hospital of Peoples Liberation Army | Tian G.,First Affiliated Hospital of General Hospital of Peoples Liberation Army
Chinese Medical Journal

Background Dendritic cells (DCs) are the most important professional antigen presenting cells that play a key role in initiating adaptive immune responses. The depletion and dysfunction of DCs contribute to the development of immunodeficiency or immunoparalysis in some lung diseases. In the present study, we investigated the effects of Fms-like tyrosine kinase-3 ligand (Flt3L) administration in vivo on lung DCs expansion to provide an experimental basis of Flt3L used as a potential therapeutic agent for the related lung disorders. Methods Balb/c mice were randomly divided into Flt3L group (n=10) and control group (n=10). Each mouse in the Flt3L group received subcutaneous administration of Flt3L at a dose of 10 μg once daily for nine consecutive days. Lung histology was observed, and CD11c and CD205 were immunologically labeled in lung tissue sections. Low-density lung cells were separated by density gradient centrifugation, and then subsets and MHC-II/I-Ad expression of DCs were analyzed by flow cytometry. Results In the Flt3L group the number and density of DC-like cells were markedly increased compared with the control group, mainly distributed in the alveolar septa. Immunological labeling in situ found that there were significantly higher numbers of CD11c+ and CD205+ DCs in lung mesenchymal tissue (P <0.05), where they formed a denser reticular formation. Flow cytometry analysis demonstrated that the proportions of myeloid CD11c+CD11b+ DCs and plasmacytoid CD11c+CD45R/B220+ DCs in the low-density lung cells in the Flt3L group were significantly higher compared with the control group; showing 3.17- and 3.3-fold increase respectively (P <0.05). The proportion of CD11c+ DCs expressing MHC-II/I-Ad+ was significantly increased, with a 2.7-fold increase as compared with the control group (P <0.05). Conclusions Flt3L administration in vivo induces lung DCs expansion, favoring myeloid and plasmacytoid DC subsets, which are phenotypically more mature. Flt3L may be useful in the therapy to augment immune function of the lung. Source

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