Institute Finlay

Havana, Cuba

Institute Finlay

Havana, Cuba
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Pino J.A.,Food Industry Research Institute | Mendiola J.,Institute of Tropical Medicine | Echemendia O.A.,Institute Finlay
Journal of Essential Oil Research | Year: 2011

The leaf essential oil of Tagetes lucida Cav. (Asteraceae) from Cuba has been obtained by hydrodistillation and analyzed by GC-FID and GC/MS. Forty volatile compounds were identified, of which estragole (96.8%) was the major constituent. The antioxidant capacity of this essential oil was measured by two different in vitro assays (DPPH and TBARS) and significant activities were evidenced. The preliminary screening of its antiplasmodial, antibacterial, antifungal and antiviral activities was carried out against Plasmodium berghei, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Candida albicans, Acinetohacterlwoffi, Enterobacter aerogenes and against strains HHV 1 and HHV 2. The results showed a moderate activity against P. berghei and E. coli. © 2011 Allured Business Media.


Tirado Y.,Institute Finlay | Puig A.,Institute Finlay | Alvarez N.,Institute Finlay | Borrero R.,Institute Finlay | And 14 more authors.
Tuberculosis | Year: 2016

Tuberculosis (TB) remains an important cause of mortality and morbidity. The TB vaccine, BCG, is not fully protective against the adult form of the disease and is unable to prevent its transmission although it is still useful against severe childhood TB. Hence, the search for new vaccines is of great interest. In a previous study, we have shown that proteoliposomes obtained from Mycobacterium smegmatis (PLMs) induced cross reactive humoral and cellular response against Mycobacterium tuberculosis (Mtb) antigens. With the objective to evaluate the protective capability of PLMs, a murine model of progressive pulmonary TB was used. Animals immunized with PLMs with and without alum (PLMs/PLMsAL respectively) showed protection compared to non-immunized animals. Mice immunized with PLMsAL induced similar protection as that of BCG. Animals immunized with BCG, PLMs and PLMsAL showed a significant decrease in tissue damage (percentage of pneumonic area/lung) compared to non-immunized animals, with a more prominent effect in BCG vaccinated mice. The protective effect of the administration of PLMs in mice supports its future evaluation as experimental vaccine candidate against Mtb. © 2016 Elsevier Ltd


Tirado Y.,Institute Finlay | Puig A.,Institute Finlay | Alvarez N.,Institute Finlay | Borrero R.,Institute Finlay | And 13 more authors.
Human Vaccines and Immunotherapeutics | Year: 2015

Tuberculosis (TB) is one of the most important causes of mortality and morbidity due to infectious diseases. BCG, the vaccine in use, is not fully protective against TB. In a previous study, we have shown that proteoliposomes (outer membrane extracts), obtained from BCG (PLBCG) were able to induce humoral immune responses against Mycobacterium tuberculosis (Mtb) antigens. With the objective to evaluate the protective capability of PLBCG alone or as a booster with BCG, a murine model of progressive pulmonary TB was used. Animals immunized with PLBCG adjuvanted with alum (PLBCG-Al) showed similar protection to that conferred by BCG. The group immunized with PLBCG-Al as a booster to BCG gave superior protection than BCG as evidenced by a reduction of bacterial load in lungs 2 months after infection with Mtb. Animals immunized with BCG, PLBCG-Al and this formulation as a booster of BCG, showed a significant decrease of tissue damage (percentage of pneumonic area/lung) compared with non-immunized animals. These results demonstrate that immunization with PLBCG-Al alone or as a booster to BCG induce appropriate protection against challenge with Mtb in mice and support the future evaluation of PLBCG as a promising vaccine candidate against Mtb. © 2015 Taylor & Francis Group, LLC.


PubMed | Universiti Sains Malaysia, National Institute of Medical science and Nutrition Salvador Zubiran and Institute Finlay
Type: | Journal: Tuberculosis (Edinburgh, Scotland) | Year: 2016

Tuberculosis (TB) remains an important cause of mortality and morbidity. The TB vaccine, BCG, is not fully protective against the adult form of the disease and is unable to prevent its transmission although it is still useful against severe childhood TB. Hence, the search for new vaccines is of great interest. In a previous study, we have shown that proteoliposomes obtained from Mycobacterium smegmatis (PLMs) induced cross reactive humoral and cellular response against Mycobacterium tuberculosis (Mtb) antigens. With the objective to evaluate the protective capability of PLMs, a murine model of progressive pulmonary TB was used. Animals immunized with PLMs with and without alum (PLMs/PLMsAL respectively) showed protection compared to non-immunized animals. Mice immunized with PLMsAL induced similar protection as that of BCG. Animals immunized with BCG, PLMs and PLMsAL showed a significant decrease in tissue damage (percentage of pneumonic area/lung) compared to non-immunized animals, with a more prominent effect in BCG vaccinated mice. The protective effect of the administration of PLMs in mice supports its future evaluation as experimental vaccine candidate against Mtb.

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