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Havana, Cuba

Pino J.A.,Food Industry Research Institute | Mendiola J.,Institute of Tropical Medicine | Echemendia O.A.,Institute Finlay
Journal of Essential Oil Research | Year: 2011

The leaf essential oil of Tagetes lucida Cav. (Asteraceae) from Cuba has been obtained by hydrodistillation and analyzed by GC-FID and GC/MS. Forty volatile compounds were identified, of which estragole (96.8%) was the major constituent. The antioxidant capacity of this essential oil was measured by two different in vitro assays (DPPH and TBARS) and significant activities were evidenced. The preliminary screening of its antiplasmodial, antibacterial, antifungal and antiviral activities was carried out against Plasmodium berghei, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Candida albicans, Acinetohacterlwoffi, Enterobacter aerogenes and against strains HHV 1 and HHV 2. The results showed a moderate activity against P. berghei and E. coli. © 2011 Allured Business Media.

Tirado Y.,Institute Finlay | Puig A.,Institute Finlay | Alvarez N.,Institute Finlay | Borrero R.,Institute Finlay | And 13 more authors.
Human Vaccines and Immunotherapeutics | Year: 2015

Tuberculosis (TB) is one of the most important causes of mortality and morbidity due to infectious diseases. BCG, the vaccine in use, is not fully protective against TB. In a previous study, we have shown that proteoliposomes (outer membrane extracts), obtained from BCG (PLBCG) were able to induce humoral immune responses against Mycobacterium tuberculosis (Mtb) antigens. With the objective to evaluate the protective capability of PLBCG alone or as a booster with BCG, a murine model of progressive pulmonary TB was used. Animals immunized with PLBCG adjuvanted with alum (PLBCG-Al) showed similar protection to that conferred by BCG. The group immunized with PLBCG-Al as a booster to BCG gave superior protection than BCG as evidenced by a reduction of bacterial load in lungs 2 months after infection with Mtb. Animals immunized with BCG, PLBCG-Al and this formulation as a booster of BCG, showed a significant decrease of tissue damage (percentage of pneumonic area/lung) compared with non-immunized animals. These results demonstrate that immunization with PLBCG-Al alone or as a booster to BCG induce appropriate protection against challenge with Mtb in mice and support the future evaluation of PLBCG as a promising vaccine candidate against Mtb. © 2015 Taylor & Francis Group, LLC.

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