Figueira da Foz Hospital

Figueira da Foz, Portugal

Figueira da Foz Hospital

Figueira da Foz, Portugal
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Pinto L.,Polytechnic Institute of Coimbra | Gomes F.,Polytechnic Institute of Coimbra | Carvalhais P.,Orthopedist at Figueira da Foz Hospital | Roseiro T.,Figueira da Foz Hospital | Roseiro L.,Polytechnic Institute of Coimbra
ENBENG 2017 - 5th Portuguese Meeting on Bioengineering, Proceedings | Year: 2017

In the context of orthopedic, the use of plantar pressure distribution can be important as a complementary method of evaluation and diagnosis, as in the follow-up of patients. The present study has its framework in the context of the distribution of plantar pressure in patients with hallux valgus pathology, with indication for surgical treatment. The work involves gait analysis in nine volunteers with surgery indication. The registered data was obtained in two moments: the day before surgery and ninety days after surgery. The analysis of the results shows that pedobarographic pressure analysis may play an important role in helping clinical decision-making, as well as follow-up in the postoperative phase, allowing the study and evaluation of the surgical proceedings and correlating the clinical evolution with the changes in pedobarography maps. © 2017 IEEE.


Caseiro A.,University of Aveiro | Caseiro A.,Polytechnic Institute of Coimbra | Ferreira R.,University of Aveiro | Quintaneiro C.,Figueira da Foz Hospital | And 4 more authors.
Clinical Biochemistry | Year: 2012

Objectives: We aimed to disclose the proteolytic events underlying type 1 diabetes and related complication through protease profiling in the bodily fluids serum, urine and saliva. Design and methods: Zymography followed by LC-MS/MS was performed for protease identification and quantitative comparison of proteolytic activity between healthy, type 1 diabetic patients with no complications and with retinopathy and nephropathy. Western blotting was also accomplished for MMP-9 and MMP-2 identification and expression analysis. Results: Only MMP-2 and MMP-9 were observed in serum with significantly increased levels and activity observed in diabetic patients. In urine and saliva other proteases besides MMPs were identified by MS and presented disease-dependent activity variations. Among these are complex MMP-9/Neutrophil gelatinase-associated lipocalin, aminopeptidase N, azurocidin and kallikrein 1 with more activity noticed in type 1 diabetes patients with nephropathy and/or retinopathy. Conclusion: Our data highlight the usefulness of urine and saliva for the monitoring of type-1 diabetes-related proteolytic events, where aminopeptidase N, azurocidin and kallikrein 1 appear as promising screening targets for type 1 diabetes-related complications. © 2012 The Canadian Society of Clinical Chemists.


Caseiro A.,University of Aveiro | Caseiro A.,Polytechnic Institute of Coimbra | Barros A.,University of Aveiro | Ferreira R.,University of Aveiro | And 7 more authors.
Translational Research | Year: 2014

Diabetes mellitus is a chronic metabolic disease with multiple complications, and its successful management requires early diagnosis, to allow timely interventions. Here, we have comprehensively analyzed the proteome changes in urine of type 1 diabetic subjects with and without complications such as retinopathy and nephropathy. gel electrophoresis combined to liquid chromatography-tandem mass spectrometry (GeLC-MS/MS) analysis of midstream urine highlighted the mechanisms involved in disease pathogenesis as, for instance wound healing and blood coagulation in all diabetics or altered ganglioside metabolism in retinopathy, and also some urinary proteins with potential diagnosis value. From these, gelsolin and antithrombin-III appear as promising diagnosis markers for type 1 diabetes mellitus (T1DM), whereas ephrin type-B receptor 4 and vitamin K-dependent protein Z seem to be promising markers for advanced T1DM disease state presenting retinopathy and nephropathy (T1DM-R + N). Data also suggest urinary ganglioside GM2 activator and beta-hexosaminidase subunit beta as potential urinary markers of retinopathy in diabetics. Taken together, the present exploratory urinary proteomic analysis might be seen as an important starting point for studies targeting specific urinary proteins aimed at the implementation of new biomarkers for the early detection of T1DM-related microvascular complications. © 2014 Mosby, Inc. All rights reserved.


Caseiro A.,University of Aveiro | Caseiro A.,Polytechnic Institute of Coimbra | Ferreira R.,University of Aveiro | Padrao A.,University of Aveiro | And 5 more authors.
Journal of Proteome Research | Year: 2013

In the present study, we applied iTRAQ-based quantitative approach to explore the salivary proteome and peptidome profile in selected subjects with type 1 diabetes, with and without microvascular complications, aiming to identify disease-related markers. From a total of 434 distinct proteins, bactericidal/permeability-increasing protein-like 1 and pancreatic adenocarcinoma up-regulated factor were found in higher levels in the saliva of all patients while increased content of other proteins like alpha-2- macroglobulin, defensin alpha 3 neutrophil-specific, leukocyte elastase inhibitor, matrix metalloproteinase-9, neutrophil elastase, plastin-2, protein S100-A8 and protein S100-A9 were related with microvascular complications as retinopathy and nephropathy. Protein-protein interaction network analysis suggests the functional clusters defense, inflammation and response to wounding as the most significantly associated with type 1 diabetes pathogenesis. Peptidome data not only support a diabetes-related higher susceptibility of salivary proteins to proteolysis (mainly of aPRP, bPRP1 and bPRP2), but also evidenced an increased content of some specific protein fragments known to be related with bacterial attachment and the accumulation of phosphopeptides involved in tooth protection. Overall, the salivary protein and peptide profile highlights the importance of the innate immune system in the pathogenesis of type 1 diabetes mellitus and related complications. This study provides an integrated perspective of salivary proteome and peptidome that should be further explored in future studies targeting specific disease markers. © 2013 American Chemical Society.


PubMed | Figueira da Foz Hospital, Polytechnic Institute of Coimbra and University of Aveiro
Type: Journal Article | Journal: Translational research : the journal of laboratory and clinical medicine | Year: 2014

Diabetes mellitus is a chronic metabolic disease with multiple complications, and its successful management requires early diagnosis, to allow timely interventions. Here, we have comprehensively analyzed the proteome changes in urine of type 1 diabetic subjects with and without complications such as retinopathy and nephropathy. gel electrophoresis combined to liquid chromatography-tandem mass spectrometry (GeLC-MS/MS) analysis of midstream urine highlighted the mechanisms involved in disease pathogenesis as, for instance wound healing and blood coagulation in all diabetics or altered ganglioside metabolism in retinopathy, and also some urinary proteins with potential diagnosis value. From these, gelsolin and antithrombin-III appear as promising diagnosis markers for type 1 diabetes mellitus (T1DM), whereas ephrin type-B receptor 4 and vitamin K-dependent protein Zseem to be promising markers for advanced T1DM disease state presenting retinopathy and nephropathy (T1DM-R+N). Data also suggest urinary ganglioside GM2 activator and beta-hexosaminidase subunit beta as potential urinary markers ofretinopathy in diabetics. Taken together, the present exploratory urinary proteomicanalysis might be seen as an important starting point for studies targeting specific urinary proteins aimed at the implementation of new biomarkers for the early detection of T1DM-related microvascular complications.


Costa F.D.,Figueira da Foz Hospital | Costa F.D.,University of Coimbra | Andrade D.R.,University of Coimbra | Cunha F.I.,Figueira da Foz Hospital | Fernandes A.,Figueira da Foz Hospital
BMJ Case Reports | Year: 2015

Acute neonatal parotitis (ANP) is a rare condition, characterised by parotid swelling and other local inflammatory signs. The most common pathogen is Staphylococcus aureus, but other organisms can be implicated. We describe the case of a 13-day-old term newborn, previously healthy, with late-onset group B Streptococcus (GBS) bacteraemia with ANP, who presented with irritability, reduced feeding and tender swelling of the right parotid. Laboratory evaluation showed neutrophilia, elevated C reactive protein and procalcitonin, with normal serum amylase concentration. Ultrasound findings were suggestive of acute parotitis. Empiric antibiotic therapy was immediately started and adjusted when culture results became available. The newborn was discharged after 10 days, with clinical improvement within the first 72 h. Although S. aureus is the most common pathogen implicated in ANP, GBS should be included in the differential diagnosis. Copyright 2015 BMJ Publishing Group. All rights reserved.


PubMed | Figueira da Foz Hospital and University of Coimbra
Type: | Journal: BMJ case reports | Year: 2015

Acute neonatal parotitis (ANP) is a rare condition, characterised by parotid swelling and other local inflammatory signs. The most common pathogen is Staphylococcus aureus, but other organisms can be implicated. We describe the case of a 13-day-old term newborn, previously healthy, with late-onset group B Streptococcus (GBS) bacteraemia with ANP, who presented with irritability, reduced feeding and tender swelling of the right parotid. Laboratory evaluation showed neutrophilia, elevated C reactive protein and procalcitonin, with normal serum amylase concentration. Ultrasound findings were suggestive of acute parotitis. Empiric antibiotic therapy was immediately started and adjusted when culture results became available. The newborn was discharged after 10days, with clinical improvement within the first 72h. Although S. aureus is the most common pathogen implicated in ANP, GBS should be included in the differential diagnosis.

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