Hu S.,Chinese PLA General Hospital |
Lin Z.-L.,Chinese PLA General Hospital |
Liu R.,Fifth Hospital of Harbin |
Ma L.,Armed Police General Hospital |
And 4 more authors.
Journal of Parenteral and Enteral Nutrition | Year: 2016
Background: Recent studies have suggested that pyruvate-enriched oral rehydration solution (Pyr-ORS) may be superior to the standard bicarbonate-based ORS in the protection of intestine from ischemic injury. The aim of this study was to compare the effects of Pyr-ORS with citrate-enriched ORS (Cit-ORS) on the intestinal hypoxia-inducible factor-1 (HIF-1)-erythropoietin (EPO) signaling pathway for enteral rehydration in a rat model of burn injury. Methods: Rats were randomly assigned to 4 groups (N = 20, 2 subgroups each: n = 10): scald sham (group SS), scald with no fluid resuscitation (group SN), scald and resuscitation with enteral Cit-ORS (group SC), and scald and resuscitation with enteral Pyr-ORS (group SP). At 2.5 and 4.5 hours after a 35% total body surface area (TBSA) scald, intestinal mucosal blood flow (IMBF), contents of HIF-1, EPO, endothelial nitric oxide synthase (eNOS), nitric oxide (NO), barrier protein (ZO-1), levels of serum diamine oxidase (DAO), and intestinal mucosal histology injury score were determined. Results: Serum DAO activities in the scalded groups were significantly elevated, but less raised in group SP than in group SC, at 2.5 hours and at 4.5 hours after the scald. Further, group SP more profoundly preserved intestinal HIF-1 expression compared with group SC at the 2 time points. Compared with group SC, group SP had markedly elevated intestinal EPO, eNOS, and NO levels at the same time points, respectively (P <.05). Similarly, IMBF and ZO-1 levels were significantly higher in group SP than in group SC. Intestinal mucosal histopathological scores were statistically higher at 2.5 hours and 4.5 hours after scalding but were more attenuated in group SP than in group SC (P <.05). Immunofluorescence expression of intestinal mucosal ZO-1 was consistent with the above changes. The above parameters were also significantly different between groups SC and SN (all P <.05). Conclusion: Pyr-ORS provides a superior option to Cit-ORS for the preservation of intestinal blood flow and barrier function and the attenuation of histopathological alterations in enteral resuscitation of rats with burn injury. Its underlying mechanism may be closely related to the pyruvate in activation of intestinal HIF-1-EPO signaling cascades. © 2014 American Society for Parenteral and Enteral Nutrition.
Liu R.,Fifth Hospital of Harbin |
Hu X.-H.,Chinese PLA General Hospital |
Hu X.-H.,University of Sydney |
Wang S.-M.,Heilongjiang University of Chinese Medicine |
And 5 more authors.
Burns | Year: 2016
Background To investigate whether pyruvate-enriched oral rehydration solution (Pyr-ORS), compared with citrate-enriched ORS (Cit-ORS), improves hemodynamics and organ function by alleviating vasopermeability and plasma volume loss during intra-gastric fluid rehydration in dogs with severe burn. Methods Forty dogs subjected to severe burn were randomly divided into four groups (n = 10): two oral rehydrated groups with Pyr-ORS and Cit-ORS (group PR and group CR), respectively, according to the Parkland formula during the first 24 h after burns. Other two groups were the intravenous (IV) resuscitation (group VR) with lactated Ringer's solution with the same dosage and no fluid rehydration (group NR). During the next 24 h, all groups received the same IV infusion. The hemodynamics, plasma volume, vasopermeability and water contents and function of various organs were determined. Plasma levels of vascular endothelial growth factor (VEGF) and platelet activating factor (PAF) were detected by ELISA. Results Hemodynamics parameters were significantly improved in group PR superior to group CR after burns. Levels of VEGF and PAF were significantly lower in group PR than in group CR. Organ function parameters were also greatly preserved in group PR, relative to groups CR and NR. Lactic acidosis was fully corrected and survival increased in group PR (50.0%), compared to group CR (20.0%). Conclusion Pyr-ORS was more effective than Cit-ORS in improving hemodynamics, visceral blood perfusion and organ function by alleviating vasopermeability-induced visceral edema and plasma volume loss in dogs with severe burn. © 2016 Elsevier Ltd and ISBI. All rights reserved.
Sun B.,University of California at Los Angeles |
Taing A.,University of California at Los Angeles |
Liu H.,CAS Technical Institute of Physics and Chemistry |
Nie G.,Fifth Hospital of Harbin |
And 9 more authors.
Journal of Nanoscience and Nanotechnology | Year: 2016
Therapeutic strategies to promote nerve cell growth and improve their functions or stimulate nerve fiber reconnection and ameliorate the loss of neuronal functions are in high demand. A disadvantage of current conventional methods, which includes injection of nerve growth factors (NGF) either systemically or in the affected area, is rapid clearance or degradation of NGF, thereby reducing the effective concentration of NGFs that can reach the damaged nerves to stimulate the healing process. To overcome this obstacle, a nanoparticle platform based on mesoporous silica nanoparticles (MSNs) was developed to not only prevent clearance and degradation of NGFs, but also deliver the NGF directly to nerve cells to promote nerve cell proliferation and neurite growth. We synthesized (NGF)-loaded MSN (MSN-NGF) with a diameter of 65 nm. MSN-NGF significantly promoted the differentiation of neuron-like PC12 cells and growth of neurites compared to NGF alone, as confirmed by MTS cell proliferation assay and optical microscopy analysis. This study shows that MSN-NGF could be an effective therapy to speed up nerve cell growth or recovery of function. © 2016 American Scientific Publishers. All rights reserved.
Shen J.,Harbin Medical University |
Shi D.,Daqing Oil field General Hospital |
Suzuki T.,RIKEN |
Xia Z.,Fifth Hospital of Harbin |
And 8 more authors.
Laboratory Investigation | Year: 2016
Retinol-binding protein 4 (RBP4) is a specific carrier for retinol in the blood. In hepatocytes, newly synthesized RBP4 associates with retinol and transthyretin and is secreted into the blood. The ternary transthyretin-RBP4-retinol complex transports retinol in the circulation and delivers it to target tissues. Rbp4-deficient mice in a mixed genetic background (129xC57BL/6J) have decreased sensitivity to light in the b-wave amplitude on electroretinogram. Sensitivity progressively improves and approaches that of wild-type mice at 24 weeks of age. In the present study, we produced Rbp4-deficient mice in the C57BL/6 genetic background. These mice displayed more severe phenotypes. They had decreased a- and b-wave amplitudes on electroretinograms. In accordance with these abnormalities, we found structural changes in these mice, such as loss of the peripheral choroid and photoreceptor layer in the peripheral retinas. In the central retinas, the distance between the inner limiting membrane and the outer plexiform layer was much shorter with fewer ganglion cells and fewer synapses in the inner plexiform layer. Furthermore, ocular developmental defects of retinal depigmentation, optic disc abnormality, and persistent hyaloid artery were also observed. All these abnormalities had not recovered even at 40 weeks of age. Our Rbp4-deficient mice accumulated retinol in the liver but it was undetectable in the serum, indicating an inverse relation between serum and liver retinol levels. Our results suggest that RBP4 is critical for the mobilization of retinol from hepatic storage pools, and that such mobilization is necessary for ocular development and visual function. © 2016 USCAP, Inc.
Hu S.,First Hospital Affiliated to the PLA General Hospital |
Zhao Z.-K.,First Hospital Affiliated to the PLA General Hospital |
Liu R.,Fifth Hospital of Harbin |
Wang H.-B.,First Hospital Affiliated to the Peoples Liberation Army General Hospital |
And 6 more authors.
World Journal of Gastroenterology | Year: 2015
AIM: To investigate whether electroacupuncture ST36 activates enteric glial cells, and alleviates gut inflammation and barrier dysfunction following hemorrhagic shock. METHODS: Sprague-Dawley rats were subjected to approximately 45% total blood loss and randomly divided into seven groups: (1) sham: cannulation, but no hemorrhage; (2) subjected to hemorrhagic shock (HS); (3) electroacupuncture (EA) ST36 after hemorrhage; (4) vagotomy (VGX)/EA: VGX before hemorrhage, then EA ST36; (5) VGX: VGX before hemorrhage; (6) α-bungarotoxin (BGT)/EA: intraperitoneal injection of α-BGT before hemorrhage, then EA ST36; and (7) α-BGT group: α-BGT injection before hemorrhage. Morphological changes in enteric glial cells (EGCs) were observed by immunofluorescence, and glial fibrillary acidic protein (GFAP; a protein marker of enteric glial activation) was evaluated using reverse transcriptase polymerase chain reaction and western blot analysis. Intestinal cytokine levels, gut permeability to 4-kDa fluorescein isothiocyanate (FITC)-dextran, and the expression and distribution of tight junction protein zona occludens (ZO)-1 were also determined. RESULTS: EGCs were distorted following hemorrhage and showed morphological abnormalities. EA ST36 attenuated the morphological changes in EGCs at 6 h, as compared with the VGX, α-BGT and HS groups. EA ST36 increased GFAP expression to a greater degree than in the other groups. EA ST36 decreased intestinal permeability to FITC-dextran (760.5 ± 96.43 ng/mL vs 2466.7 ± 131.60 ng/mL, P < 0.05) and preserved ZO-1 protein expression and localization at 6 h after hemorrhage compared with the HS group. However, abdominal VGX and α-BGT treatment weakened or eliminated the effects of EA ST36. EA ST36 reduced tumor necrosis factor-α levels in intestinal homogenates after blood loss, while vagotomy or intraperitoneal injection of α-BGT before EA ST36 abolished its anti-inflammatory effects. CONCLUSION: EA ST36 attenuates hemorrhage-induced intestinal inflammatory insult, and protects the intestinal barrier integrity, partly via activation of EGCs. © The Author(s) 2015.
Mao Y.,Fifth Hospital of Harbin |
Zhao J.,Fifth Hospital of Harbin |
Gao Y.,Fifth Hospital of Harbin
National Medical Journal of China | Year: 2015
Objective To compare the microphones organism and propofol in elderly patients with hip fracture surgen' ICU clinical effect and safety of sedation. Methods To collect 5 hospitals in Harbin in January 2014 - August 2014, 72 cases of senile spinal postoperative ICU patients, randomly divided into the propofol group (group A: 36 cases) and right beautiful mi organism group (group B: 36 cases). Group A; first of all, intravenous 1 mg/kg propofol sedation induction, according to different degree of sedation maintain propofol dosage is 0.5 to 0.5 mg · kg -1 · h -1. Group B : give the right pyrimidine load 1 mg · kg-1 · h-1 via intravenous 20 min, according to different degree of sedation sustained by intravenous pump right beauty holds 0.3 0.7 mu pyrimidine g · kg -1 · h -1. Compare two groups of patients sedation depth, ICU stay time, heart rate, blood pressure, breathing rate, increase analgesic drugs. Results Within the scope of the dose, propofol and the right supporting pyrimidine required calming effect similar to provide treatment (RamSay score > 3) ; Calm during the treatment, the right beauty pyrimidine group to the number of additional analgesics is less than the propofol group; Compared with propofol group, the right beauty pyrimidine a significant reduction in patients with ICU stay time. The incidence of adverse reactions in patients with similar between the two groups has no statistical significance (P > 0.05). Conclusion In certain dose range of ICU in elderly hip fracture patients with postoperative propofol and right the pyrimidine sedation is safe and effective.
Sun X.,Harbin Medical University |
Wang L.-m.,Harbin Medical University |
Liu Y.-x.,Harbin Medical University |
Han S.-t.,Fifth Hospital of Harbin |
He Y.,Harbin Medical University
Chinese Journal of Tissue Engineering Research | Year: 2012
BACKGROUND: Virtual reality technology applied research in the field of medicine has achieved fruitful results and shows a good prospect, it is an important tool of digital medical research. OBJECTIVE: To introduce the design and application of computer-based case simulation system with virtual reality technology. METHODS: A computer-based search was performed on CNKI database, VIP database, Wanfang database and EBSCO database for the relate articles from 1991 to 2011 with the key words of "case simulations, CCS, virtual reality" in English and "virtual reality, case simulation" in Chinese. A total of 85 articles were obtained and finally 20 articles were included according to the inclusion criteria. RESULTS AND CONCLUSION: The application of virtual reality technology in the medical field has become more and more wide. The case simulation system designed by virtual reality technology provides the medical workers a new interactive skill training methods. The simulation of the patient's disease status by the interactive computer software is realized, and the clinical course is simulated realistically. And the assessment of the practical skills for the users were achieved, including basic inspection capacity, use and judgment of the high-tech diagnostic techniques, judgment of check results, diagnostic capability, drug treatment capacity, ability to use non-drug treatment and the comprehensive treatment capacity. The virtual simulation system platform has a good prospect in medical education and clinical skills training.
Liu X.,Wuhan University of Science and Technology |
Liu J.,Wuhan University of Science and Technology |
Zhou D.,Fifth Hospital of Harbin |
Tang J.,Wuhan University of Science and Technology
2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010 | Year: 2010
In this paper, we investigate the classification of masses with texture features. We propose an improved level set method to find the boundary of a mass, based on the initial contour provided by radiologists. After the boundary of a mass is found, texture features from Gray Level Co-occurrence Matrix (GLCM) are extracted from the surrounding area of the boundary of the mass. The extracted texture features are used as the input of Linear discriminant analysis and a support vector machine to classify the mass as benign or malignant. Mammography images from DDSM were used in the experiments and the classification accuracy was evaluated using the area under the receiver operating characteristic (ROC) curve. In the proposed method the area under the ROC curve is Az =0.7. The experimental result shows the effectiveness of the proposed method. © 2010 IEEE.
Li X.,Harbin Medical University |
Lei M.,Fifth Hospital of Harbin |
Song Y.,Fifth Hospital of Harbin |
Gong K.,Fifth Hospital of Harbin |
And 3 more authors.
Gut Pathogens | Year: 2014
Background: Staphylococcus capitis is an emerging opportunistic pathogen of humans, and found as a colonizer of the human gut. Here, we report a case of S. capitis subsp. urealyticus infection. The strain LNZR-1 was isolated from the blood culture of a patient with sigmoid colon cancer. It was found to be resistant to some important antibiotics, such as linezolid, a highly effective antimicrobial against clinically important Staphylococci pathogens. However, data on the genetic resistance mechanisms in S. capitis subsp. urealyticus are only sparsely available. Results: The draft genome of S. capitis subsp. urealyticus strain LNZR-1 was sequenced by using next-generation sequencing technologies. Sequence data assembly revealed a genome size of 2,595,865 bp with a G∈+∈C content of 32.67%. Genome annotation revealed the presence of antibiotic resistance genes conferring resistance against some of the tested antibiotics as well as non-tested antibiotics. The genome also possesses a lot of genes that may be related to multidrug resistance. Whole genome comparison of the LNZR-1 with five other S. capitis strains showed that some functional regions are highly homologous between the six assemblies made herein. The LNZR-1 genome has high similarity with the genomes of the strains VCU116 and CR01, although some short stretches present in the genomes of strains VCU116 and CR01 were absent in the strain LNZR-1. Conclusions: The presence of a plethora of genes responsible for antibiotic resistance suggests that strain LNZR-1 could present a potential threat to human health. The comparative genomic analysis of S. capitis strains presented in this study is important for better understanding of multidrug resistance in S. capitis. © 2014 Li et al.; licensee BioMed Central.
Zhen X.-g.,Fifth Hospital of Harbin |
Wang S.-q.,Fifth Hospital of Harbin |
Yu Q.,Fifth Hospital of Harbin
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2011
BACKGROUND: The ideal drugs therapy for osteolysis may not only relieve the pain, but also save medical costs. OBJECTIVE: To investigate the 5-year effect of alendronate and alfacalcidol combination on preventing periprosthetic osteolysis after total hip arthroplasty. METHODS: Seventy-five patients undergoing cemented total hip replacement after femoral neck fracture were randomly divided into 3 groups: the treatment group was given alendronate and alfacalcidol oral combination therapy, the control group was treated with compound amino acid chelate joint capsule of oral calcium therapy, and the blank control group with normal diet. Periprosthetic bone mineral density and average Harris score were examined at the 1st, 3rd, 5th years after the operation, and imaging evaluation after 5 years. RESULTS AND CONCLUSION: Three and five years after hip replacement, bone density in treatment group was significantly higher than that in the control group (P < 0.05); 5 years after surgery, bone dissolution rate of the treatment group was lower than that of the control group and the blank control group (P < 0.05). The results showed that the combination of alendronate and alfacalcidol played a positive role in preventing the occurrence of osteolysis after hip prosthesis replacement.