Abano Terme, Italy
Abano Terme, Italy

Time filter

Source Type

Patent
Fidia Farmaceutici S.P.A. | Date: 2016-08-02

A process for preparing Streptomyces koganeiensis ATCC 31394 hyaluronidase includes obtaining hyaluronidase having molecular weight of 21.6 kDalton, which has hyaluronidase activity and stability markedly higher than those of the hyaluronidase obtained from such microorganism to date.


The object of the present invention relates to the new and surprising use of sulfated hyaluronic acid (HAS) as regulator agent of the cytokine activity (pro- and anti-inflammatory) and consequently the use of HAS for the preparation of a new medicine for topic use in the prevention and treatment of pathologies associated with the activation and/or deficiency of cytokines of a pro- and anti-inflammatory nature. The Applicant has in fact discovered the exclusive capacity of HAS in modulating the activity of these particular proteins, it has studied the action mechanism and demonstrated the substantial difference between the different sulfated types known in the state of the art, but above all it has demonstrated an unexpectedly high activity of HAS vs different types and strains of Herpes virus, Cytomegalovirus and the virus of vesicular stomatitis. Finally, a further object of the present invention is the use of HAS as a skin absorption promoter of drugs of an anti-inflammatory nature.


Phospholipid liposomes comprising at least one polyunsaturated fatty acid selected in the group consisting of dihomo-gamma-linolenic acid (DGLA), linoleic acid, gamma-linolenic acid, arachidonic acid, docosatetraenoic acid, a plant estrogen selected from equol, genistein, daidzein, glycitein; a compound able to increase cation capacity of the liposome selected from essential amino acids, carnitine and derivatives thereof; a stabilizer selected from cholesterol, cholesterol sulphate and stearylamine, for use in the treatment of alopecia, baldness, hair loss, and related pharmaceutical compositions containing them as active ingredients in association with suitable excipients and/or diluents.


Grant
Agency: European Commission | Branch: H2020 | Program: MSCA-RISE | Phase: MSCA-RISE-2014 | Award Amount: 567.00K | Year: 2015

Cancer is a leading cause of mortality within the aging European population. Therapeutic targeting is hampered by the complexity of the disease, which includes not only molecular changes within the tumor cell itself, but also within its microenvironment. Tumor angiogenesis, tumor-stroma interactions, interactions with immune cells, with the extracellular matrix and cancer stem cell niches allow for malignant cell survival and promote metastasis, the leading cause for cancer-associated mortality. Proteoglycans (PGs) and glycosaminoglycans (GAGs) structurally diverse carbohydrates of the extracellular matrix and cell surfaces - have emerged as novel biomarkers and molecular players both within tumor cells and their microenvironment, as they integrate signals from growth factors, chemokines and integrins, and cell-cell as well as matrix adhesion. Importantly, their expression is dysregulated in numerous tumor entities, and has been shown to modulate each of the hallmarks of cancer as defined by Hanahan and Weinberg (Cell 2011). We hypothesize that dysregulated function of PGs and GAGs simultaneously affects all molecular steps towards cancer metastasis as a general principle applicable to multiple tumor entities. Pharmacological modulation of their function thus emerges as an attractive multitargeted antitumoral approach which simultaneously acts at multiple levels of disease progression. Besides providing extensive knowledge transfer and training for researchers, the combined expertise of the GLYCANC consortium aims at performing a detailed structural analysis of PG and GAG glycans in disease using state-of-the art methodology, analysing their regulation via epigenetic mechanisms and microRNAs, and elucidating molecular mechanisms underlying aberrant PG and GAG function. GLYCANC will lead to a deeper understanding of glycan structures and glycan-dependent mechanisms promoting cancer progression, providing the basis for rational multitargeted anticancer approaches.


Patent
FIDIA FARMACEUTICI S.p.A. | Date: 2016-12-14

The object of the present invention relates to sulfated hyaluronic acid (HAS) for use as regulator agent of the cytokine activity (pro- and anti-inflammatory) and consequently the use of HAS for the preparation of a medicine for the prevention and treatment of pathologies associated with the activation and/or deficiency of cytokines of a pro- and anti-inflammatory nature, including vascular pathologies relating to activation of TNF, IL-1 and IL-6, skin diseases associated with IL-10 deficit, certain viral or immune related diseases, or pathologies associated to the activation of osteoclasts. The Applicant has discovered its capacity in modulating the activity of these particular proteins, and has demonstrated the substantial difference between the different sulfated types known in the state of the art, but above all it has demonstrated an unexpectedly high activity of HAS vs different types and strains of Herpes virus, HIV, Cytomegalovirus and the virus of vesicular stomatitis.


The object of the present invention relates to sulfated hyaluronic acid (HAS) for topical use in the prevention and/or treatment of skin pathologies associated with damage to the endothelium and/or the wall of blood vessels, in the treatment of clots of fibrin and thrombi which are formed at the skin level; as a skin absorption promoter of pharmacologically and/or biologically active agents and in the prevention and/or treatment of Herpes Simplex labialis and Herpes genitalis, the virus of Vesicular Stomatitis and Cytomegalovirus.


A method of producing hyaluronic acid (HA) in Escherichia coli and Bacillus megaterium through episomal plasmid vectors wherein the gene is under the control of strong promoter T7, preferably under the control of strong promoter T7 of bacteriophage T7, and a system for the selection of stable bacterial strains producing high levels of hyaluronic acid, are provided.


Patent
Fidia Farmaceutici S.P.A. | Date: 2014-11-17

The present invention discloses pharmaceutical compositions comprising ester derivatives of hyaluronic acid for use as a topical treatment in disorders of the vaginal mucosa characterised by loss of elasticity and hydration, such as vaginal dryness and/or atrophic vaginitis; said compositions can also be used successfully to lubricate the genital mucosa.


The present invention relates to pharmaceutical formulations containing a combination of specific high-molecular weight hyaluronic acid derivatives and chondroitin sulfate to be used in the treatment of osteoarthritis, of subchondral damage, osteoporosis, synovitis, tenosynovitis, tendinitis, tendinosis, as an intra-articular washing liquid and as a viscous substitute of synovial fluid following osteochondral surgery. These formulations are also suitable for the treatment of interstitial cystitis.


The present invention relates to a method for the production of hyaluronic acid (HA) in Bacillus subtilis and Escherichia coli through plasmid vectors wherein the gene is under the control of strong promoter Pgrac, and a system for the selection of stable bacterial strains for the production of high levels of hyaluronic acid.

Loading Fidia Farmaceutici SpA collaborators
Loading Fidia Farmaceutici SpA collaborators