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Joondalup, Australia

Yap K.,Fertility North | Matson P.,Fertility North | Matson P.,Edith Cowan University
Asian Pacific Journal of Reproduction | Year: 2012

Objective: To explore a method of semen storage prior to assessment of sperm DNA fragmentation. Methods: This study examined a simplified alternative of air-drying semen on a microscope slide and reconstituting in seminal plasma prior to assessment of sperm DNA fragmentation using the halosperm® G2 kit. Results: It showed that semen could be air-dried and stored overnight at room temperature with no detrimental effect on DNA quality. A significant correlation between results existed for 20 semen samples both air-dried and snap-frozen in liquid nitrogen (. r=0.982, P=0.000). A mean difference between the results of only -1.98% confirmed the effectiveness of air-drying compared to snap-freezing. Conclusions: Future studies to refine this technique are required on the effect of extrinsic factors such as the choice of reconstituting medium, and stability over an extended time-frame at different temperatures. © 2012 Hainan Medical College.

Matson P.,Fertility North | Matson P.,Edith Cowan University | Tardif S.,University of Dundee | Tardif S.,Minitube Of America
Reproductive Biology | Year: 2012

Non-animal macromolecules (Select Phytone™ UF, wheat peptone, dextran 40, hydroxyethyl starch and methyl cellulose) as an alternative medium supplement for human spermatozoa were compared to bovine serum albumin. Select Phytone™ UF and wheat peptone discolored the medium and smelled like broth, making them unlikely to be acceptable for clinical use, whilst the others were colorless and odorless. All supplements were effective in the yield of spermatozoa isolated by swim-up technique, and maintenance of sperm motility. In summary, there are non-animal macromolecules that will support short-term sperm culture. © 2012 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn.

Zuvela E.,Fertility Specialists WA | Junk S.,Fertility Specialists WA | Moska N.,Hollywood Fertility Center | Matson P.,Fertility North | Matson P.,Edith Cowan University
Reproductive Biology | Year: 2011

The usefulness of latex beads of defined concentration was assessed as a substitute for sperm in the performance of External Quality Assurance (EQA) and Internal Quality Control (IQC) of semen analysis. Within the EQA programme, mean±SEM bias (%) was significantly reduced in 2007 compared to 2002 for both specialist (6.0%±5.4% vs. 55.0%±5.9%) and non-specialist (18.4%±5.9% vs. 90.9%±13.4%) laboratories (both p<0.0001), indicating improved accuracy over time. Within the IQC programme, the beads were used in the appraisal of two scientists, one experienced and one inexperienced, against a known standard. Beads were also used to calibrate eleven counting chambers, resulting in one old chamber being discarded due to its poor performance. The present study has shown that the use of a defined concentration of beads is an excellent adjunct to IQC and EQA programmes enabling the performance of both people and equipment to be assessed in an objective manner.

Hadlow N.,Western Diagnostic Pathology | Hadlow N.,University of Western Australia | Longhurst K.,Western Diagnostic Pathology | McClements A.,Fertility North | And 4 more authors.
Fertility and Sterility | Year: 2013

Objective: To assess the variability of antimüllerian hormone (AMH) concentrations in women with "adequate ovarian reserve" during unstimulated menstrual cycles and to determine the impact on clinical classifications. Design: Pilot cohort study. Setting: Private fertility clinic. Patient(s): Twelve consecutive women (aged 29 to 43 years) referred to a fertility service, with AMH measurements repeated throughout unstimulated cycle, and who had at least one AMH measurement indicating "adequate ovarian reserve." Intervention(s): None. Main Outcome Measure(s): AMH concentrations assessed in 82 serum samples from 12 women compared against the published cutoffs for reduced ovarian reserve and for risk of excessive response to ovarian stimulation. Result(s): Over half the women (7 of 12) were reclassified as a result of testing AMH concentrations at different phases of the menstrual cycle. Over one-third (4 or 5 of 12) crossed a cutoff for reduced ovarian reserve; 2 of 12 crossed cutoffs predicting hyperstimulation. There was a statistically significant change in AMH concentration according to the day of the cycle, with a negative trend of the median AMH concentration from the follicular to luteal phase. The maximum change in median AMH concentration over cycle was 7.9 pmol/L, and the mean difference between the maximum and minimum AMH was 6.7 pmol/L. Conclusion(s): In this pilot study, the AMH concentration varied during menstrual cycle, and the clinical classification of the ovarian response was altered. © 2013 American Society for Reproductive Medicine.

McEvoy A.,Edith Cowan University | Roberts P.,Edith Cowan University | Yap K.,Fertility North | Matson P.,Edith Cowan University
Fertility and Sterility | Year: 2014

Intervention(s): Comparison of sperm DNA fragmentation levels (DFLs) using fresh, snap-frozen and air-dried semen, with air-dried samples stored at different temperatures and time periods to assess DNA stability.Objective: To develop a simple, convenient, and stable storage method for semen before DNA fragmentation testing.Design: Experimental cross-sectional study.Setting: Fertility clinic.Patient(s): 164 male partners of infertile couples.Main Outcome Measure(s): DFL determined by Halosperm G2 kit.Result(s): Results are expressed as mean ± standard error of the mean. The DFLs from fresh and air-dried semen gave comparable results (1.08% ± 0.65%), and from snap-frozen and fresh samples a statistically significant difference (5.5% ± 1.09%). Air-dried semen stored at room temperature for 7 days had a statistically significantly higher DFL compared with semen stored overnight (46.29% ± 9.12%). Samples stored at 4°C for 7 days or 1 day showed no statistically significant difference (0.83% ± 0.82%). DFLs from samples stored for either 1 or 30 days at 4°C showed a statistically significant difference (19.59% ± 5.72%); those stored at -22°C showed no statistically significant difference (0.68% ± 0.53%).Conclusion(s): Air-drying semen is a simple and stable storage method for up to 1 month at -22°C before DNA fragmentation testing. © 2014 American Society for Reproductive Medicine.

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