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Setti A.S.,Sapientiae Institute Educational | Figueira R.C.S.,Fertility Assisted Fertilisation Center | Braga D.P.A.F.,Sapientiae Institute Educational | Braga D.P.A.F.,Fertility Assisted Fertilisation Center | And 4 more authors.
Reproductive BioMedicine Online | Year: 2012

The aim of this prospective randomized study was to determine if the use of intracytoplasmic morphologically selected sperm injection (IMSI) is associated with gender incidence. Couples who underwent IVF-preimplantation genetic screening (PGS) cycles, as a result of advanced maternal age, were randomly allocated into two groups: intracytoplasmic sperm injection (ICSI; n = 80) or intracytoplasmic morphologically selected sperm injection (IMSI; n = 80). The incidences of genders were compared between ICSI- and IMSI-derived embryos. Considering all the biopsied embryos were characterized as normal for sex chromosome, the results showed that IMSI results in a significantly higher incidence of female embryos as compared with ICSI (65.1% versus 54.0%, respectively, P = 0.0277). After analysing only euploid embryos for the eight selected chromosomes, a significantly higher incidence of XX embryos derived from IMSI was also observed compared with ICSI cycles (66.9% versus 52.5%, respectively, P = 0.0322). This result was confirmed by logistic regression, which demonstrated a nearly 2-fold increase in euploid XX embryos derived from spermatozoa selected by high magnification (OR 1.83, 95% CI 1.05-3.35, P = 0.032). A higher proportion of morphologically normal spermatozoa analysed under high magnification seem to carry the X chromosome. The aim of this study was to determine if the use of intracytoplasmic morphologically selected sperm injection (IMSI) is associated with gender incidence. Couples who underwent IVF with preimplantation genetic screening, as a result of advanced maternal age, were randomly allocated into two groups: intracytoplasmic sperm injection (ICSI; n = 80) or intracytoplasmic morphologically selected sperm injection (IMSI; n = 80). The incidences of genders were compared between ICSI- and IMSI-derived embryos. Our results showed that a significantly higher incidence of female embryos derived from IMSI compared with ICSI cycles (66.9% versus 52.5%, respectively, P = 0.0322). This result was confirmed by logistic regression, which demonstrated a nearly 2-fold increase in female embryos derived from sperm selected by high magnification (OR 1.83, 95% CI 1.05-3.35, P = 0.032). A higher proportion of morphologically normal spermatozoa analysed under high magnification seem to carry the X chromosome. © 2012, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved. Source


Setti A.S.,Sapientiae Institute | Figueira R.C.S.,Fertility Assisted Fertilisation Center | Braga D.P.A.F.,Fertility Assisted Fertilisation Center | Colturato S.S.,Fertility Assisted Fertilisation Center | And 4 more authors.
European Journal of Obstetrics Gynecology and Reproductive Biology | Year: 2011

The aim of this study was to perform a meta-analysis of the potential effects of oocyte morphological abnormalities on ICSI outcomes. Relevant original papers reporting on the relation between oocyte morphology and ICSI outcomes were identified by searching MEDLINE, EMBASE and the Cochrane Library. The main outcome measures were fertilisation rate and embryo quality. A meta-analysis was performed and Mantel-Haenszel pooled odd ratios (ORs) with 95% confidence intervals (CIs) were calculated to express the relation between the oocyte morphology and the ICSI outcomes. A total of 14 studies reporting 3688 ICSI cycles were included. Our meta-analysis demonstrates that the probability of an oocyte becoming fertilised is significantly reduced by the presence of large IPB (OR: 0.29, CI: 0.09-0.90), large PVS (OR: 0.86, CI: 0.74-0.99), refractile bodies (OR: 0.66, CI: 0.51-0.84) or vacuoles (OR: 0.59, CI: 0.42-0.83). No other investigated morphological abnormalities demonstrated significant relationships with ICSI outcomes. Our data demonstrate that the presence of large IPB, large PVS, refractile bodies or vacuoles is associated with decreased oocyte fertilisation. Our findings might be of importance for selecting embryos for replacement because the effects of oocyte abnormalities on implantation and pregnancy rates remain unclear. Copyright © 2011 Published by Elsevier Ireland Ltd. All rights reserved. Source


Setti A.S.,Sapientiae Institute | Figueira R.D.C.S.,Fertility Assisted Fertilisation Center | Braga D.P.D.A.F.,Sapientiae Institute | Braga D.P.D.A.F.,Fertility Assisted Fertilisation Center | And 5 more authors.
Journal of Assisted Reproduction and Genetics | Year: 2011

Purpose: To evaluate whether oocyte dysmorphisms affect oocyte survival rates in an egg-cryobanking donation program. Methods: This study included 54 patients undergoing intracytoplasmic sperm injection. A total of 415 metaphase II oocytes were vitrified using the Cryotop method. Oocyte morphology was assessed immediately prior to oocyte vitrification under 400× magnification. The influence of dysmorphisms on post-thaw survival rates was assessed using regression analysis. Results were considered to be significant at the 5% critical level. Results: Oocyte survival rate was not affected by the presence of the following analysed oocyte abnormalities: increased cytoplasmic granularity, vacuoles in the ooplasm, aggregates of smooth endoplasmic reticulum in the ooplasm, large perivitelline space size, perivitelline space granularity, fragmented first polar body and zona pellucida abnormalities. Conclusions: Oocyte morphology, observed prior to vitrification, does not predict post-warming survival. The non-invasive identification of predictive markers for oocyte survival potential remains a difficult task. © 2011 Springer Science+Business Media, LLC. Source

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