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Buenos Aires, Argentina

Menendez-Helman R.J.,CONICET | Sanjurjo C.,Fertilab | Miranda P.V.,CONICET | Miranda P.V.,Santa Fe Institute
Iranian Journal of Reproductive Medicine | Year: 2015

Background: Glycosidases profusion in male reproductive fluids suggests a possible relationship with sperm function. Although Hexosaminidase (Hex) is the most active glycosidase in epididymal fluid and seminal plasma, as well as in spermatozoa, Glucosidase is considered a marker for epididymal function and azoospermia. Objective: The aim of this study was to determine Hex activity in seminal plasma from patients with normal and abnormal spermograms and analyze its correlation with seminal parameters. Materials and Methods: In this cross sectional study, seminal plasma from azoospermic, asthenozoospermic, teratozoospermic, and normozoospermic patients was analyzed for the activity of: total Hex, HexA isoform, and glucosidase. Besides, hexosamine levels were determined, and the amount of Hex was quantified by immunoblot with a specific antibody. Correlation of Hex activity with seminal parameters was also analyzed. Results: Hex activity, like glucosidase, was significantly reduced in azoospermic samples (44, 49, and 60% reduction for total Hex, HexA and glucosidase, respectively). A reduced amount of Hex in azoospermic samples was confirmed by western immunoblot. Hex activity was negatively correlated with round cells in azoospermic samples and positively correlated with motility in asthenozoospermic ones. Conclusion: The results suggested that Hex activity was reduced in azoospermic samples and this was due to a lower amount of enzyme. The correlation to seminal parameters related to particular pathologies suggests a possible relationship of Hex with fertilizing capacity. © 2015 Research and Clinical Center for Infertitlity. All rights reserved. Source


Calvo N.I.R.,Fertilab | Calvo N.I.R.,University of Buenos Aires | Studdert G.A.,Fertilab | Calandroni M.B.,Fertilab | And 3 more authors.
Ciencia del Suelo | Year: 2014

Organic carbon (CO) and anaerobically incubated nitrogen (Nan) are soil quality indicators. In addition, Nan is an estimator of nitrogen (N) supplied by organic matter mineralization, and has been used to adjust crop N fertilization rates. The aims of this study were: 1) to survey and map total CO (COT) and Nan contents in cropped soils of Buenos Aires province, and 2) evaluate the relationship between those variables at a regional scale. A total of 31.776 and 6556 soil samples taken at 0-20 cm-soil depths were used for COT and Nan, respectively. Prediction efficiencies were 79% for COT and 67% for Nan. COT content varied between 3.5 and 80.8 g kg-1, increasing from northwest to southeast of Buenos Aires. Nan concentration ranged from 12 to 260 mg kg-1, the highest values corresponding to the eastern areas decreasing to the west. This evidences the different soil N supply potential, which should be taken into account to adjust crop N fertilization rates. A significant relationship between COT and Nan (R2 = 50%) was found at the regional scale. However, the estimation of Nan content using COT would not be a recommendable procedure to diagnose N fertility. However, the maps and relationships could be used for a preliminary approach to N fertility diagnostics at the regional scale and as indicative at the field scale. © 2014, Asociacion Argentina de la Ciencia del Suelo. All rights reserved. Source

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