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Fontenelle E.,Fernandes Figueira Institute | de Almeida A.P.M.,Teaching Hospital Alvaro Alvim | Souza G.M.A.A.,Dermatologist of the Military Firefighters Corporation of the State of Rio de Janeiro
Anais Brasileiros de Dermatologia | Year: 2013

Marshall ́s syndrome is a form of acquired cutis laxa without systemic involvement, which is preceded by an inflammatory dermatitis with a neutrophilic component. We report a case of a 6-year-old boy with clinical and histopathological features of this syndrome. The etiology remains unknown and there is no definitive treatment. © 2013 by Anais Brasileiros de Dermatologia. Source


Lafouresse F.,French Institute of Health and Medical Research | Lafouresse F.,University Paul Sabatier | Lafouresse F.,French National Center for Scientific Research | Lafouresse F.,CNRS Institute of Pharmacology and Structural Biology | And 6 more authors.
Tissue Antigens | Year: 2013

T lymphocytes are key players of adaptive immune responses. Upon recognition of specific peptides presented by human leukocyte antigen (HLA) molecules on antigen presenting cells (APC), these cells execute subset-related functions such as killing, help and regulation. The ontogeny, the activation and the effector functions of T lymphocytes are all steps of T-lymphocyte life cycle that rely on high motility properties. These cells travel through the organism in a succession of steps, including entry into tissues, interstitial migration, APC scanning, synapse formation and tissue exit. Such ability is possible because of a plastic motility behavior, which is highly controlled in time and space. The molecular basis for the adaptable motility behavior of T lymphocytes is only starting to be unraveled. The scope of this review is to discuss recent data pointing to the key role of regulators of actin cytoskeleton remodeling in tuning distinct aspects of T-lymphocyte motility during their entry, residency and exit from tissues. © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. Source


Oxlade O.,Harvard University | Pinto M.,Fernandes Figueira Institute | Trajman A.,Gama Filho University | Trajman A.,McGill University | Menzies D.,McGill University
PLoS ONE | Year: 2013

Introduction: Cost effectiveness analyses (CEA) can provide useful information on how to invest limited funds, however they are less useful if different analysis of the same intervention provide unclear or contradictory results. The objective of our study was to conduct a systematic review of methodologic aspects of CEA that evaluate Interferon Gamma Release Assays (IGRA) for the detection of Latent Tuberculosis Infection (LTBI), in order to understand how differences affect study results. Methods: A systematic review of studies was conducted with particular focus on study quality and the variability in inputs used in models used to assess cost-effectiveness. A common decision analysis model of the IGRA versus Tuberculin Skin Test (TST) screening strategy was developed and used to quantify the impact on predicted results of observed differences of model inputs taken from the studies identified. Results: Thirteen studies were ultimately included in the review. Several specific methodologic issues were identified across studies, including how study inputs were selected, inconsistencies in the costing approach, the utility of the QALY (Quality Adjusted Life Year) as the effectiveness outcome, and how authors choose to present and interpret study results. When the IGRA versus TST test strategies were compared using our common decision analysis model predicted effectiveness largely overlapped. Implications: Many methodologic issues that contribute to inconsistent results and reduced study quality were identified in studies that assessed the cost-effectiveness of the IGRA test. More specific and relevant guidelines are needed in order to help authors standardize modelling approaches, inputs, assumptions and how results are presented and interpreted. © 2013 Oxlade et al. Source


Brunoro G.V.F.,Oswaldo Cruz Institute | Carvalho P.C.,Carlos Chagas Institute | Ferreira A.T.D.S.,Oswaldo Cruz Institute | Perales J.,Oswaldo Cruz Institute | And 4 more authors.
Journal of Proteomics | Year: 2015

NAF is a breast fluid that is closely related to the tumor microenvironment and a valuable sample for studying breast cancer. To perform an in-depth proteomic analysis of this sample, aliquots of a single NAF digest were analyzed by the following peptide-centric fractionation strategies: a) 30-cm reversed-phase (RP) column on-line with an LTQ-Orbitrap XL; b) off-line strong cation-exchange (SCX) column; and c) pI-based OFFGEL fractionation. All fractions from approaches (b) and (c) were further analyzed on a 10-cm RP column hyphenated to the same mass spectrometer. The RP-30. cm, SCX/RP-10. cm, and OFFGEL/RP-10. cm approaches identified 1676, 2930, and 3240 peptides, which corresponded to 193, 390 and 528 proteins, respectively. In our cumulative dataset, 4466 distinct NAF peptides corresponded to a total of 557 proteins, of which only 34% were identified by all three approaches. No exclusive protein identification was associated to the RP-30. cm approach, while SCX/RP-10. cm and OFFGEL/RP-10. cm contributed to 28 and 166 exclusive identifications, respectively. Each approach provided additional information related to energy metabolism (fermentation process/carbohydrate biosynthesis). In conclusion, the pre-fractionation platforms used were complementary for the comprehensive characterization of NAF and our work provides methodological information for future quantitative cancer-related NAF sample studies. Biological significance: High-resolution peptide separation is a sine qua non condition for achieving extensive proteome coverage. Various techniques have been employed to improve peptide fractionation prior to LC-MS/MS, thus allowing a comprehensive characterization of complex biological samples. Although fractionation efficiency is very sample-dependent, this issue is commonly overlooked, and a "cookbook" approach is routinely used during this critical step. The present study provides a systematic comparison of analytical information needed for the successful large-scale differential proteomic analysis of NAF samples from breast cancer patients, a precious and volume-limited biological sample. It reinforces the importance of optimizing sample-specific fractionation protocols for information retrieval from mass spectrometric analysis. © 2015 Elsevier B.V. Source


Moraes C.L.,State University of Rio de Janeiro | Moraes C.L.,Estacio de Sa University | Tavares Da Silva T.D.S.,State University of Rio de Janeiro | Reichenheim M.E.,State University of Rio de Janeiro | And 5 more authors.
Paediatric and Perinatal Epidemiology | Year: 2011

Summary Moraes CL, Tavares da Silva TS, Reichenheim ME, Azevedo GL, Dias Oliveira AS, Braga JU. Physical violence between intimate partners during pregnancy and postpartum: a prediction model for use in primary health care facilities. Paediatric and Perinatal Epidemiology 2011; 25: 478-486. This article offers a simple predictive model of physical intimate partner violence (PIPV) to be used by primary health care (PHC) professionals. The sample comprised 811 mothers of children <5 months old attending PHC facilities in Rio de Janeiro, Brazil. A multinomial logit model was used. Measured by the Revised Conflict Tactics Scales, PIPV was classified in three levels (absence, at least one episode during pregnancy or postpartum, and presence in both periods). Socio-economic, demographic and life style variables were considered as potential predictors. Maternal age <20 years, an education of <8 years of schooling, raising >2 children under 5, tobacco smoking, alcohol misuse and illicit drug use by the mother and/or partner, and perception of baby's ill-health were identified as predictors of PIPV. The model-projected prevalence of PIPV for pregnancy and/or postpartum was just 10.1% in the absence of these characteristics, whereas this increased to 96.4% when all the seven characteristics were present. Child, maternal and family characteristics greatly increase the likelihood of PIPV and could be used together as screening indicators. © 2011 Blackwell Publishing Ltd. Source

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