Fera Science Ltd. Fera

New York City, United States

Fera Science Ltd. Fera

New York City, United States
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Driffield M.,Fera Science Ltd Fera | Bradley E.,Fera Science Ltd Fera | Castle L.,Fera Science Ltd Fera | Lloyd A.,Fera Science Ltd Fera | And 4 more authors.
Rapid Communications in Mass Spectrometry | Year: 2015

Rationale: Plasticisers are used in the PVC gaskets of metal closures on glass jars and bottles used for foods and beverages. They may migrate and so contaminate the packed foodstuff. The plasticisers are present in a high proportion and are often a complex mixture of substances leading to time-consuming analytical methodologies. This work describes a rapid screening method to identify the plasticisers used. Methods: Analysis was carried out by direct sampling of the gaskets using atmospheric pressure solids analysis probe (ASAP) with time-of-flight (TOF) mass spectrometry (MS) using a SYNAPT G2 HDMS system. The accurate mass information collected was then compared to a user-prepared database of plasticisers to aid identification. Results: The rapid identification approach was shown to be successful for 24 gasket samples previously analysed by alternative more lengthy gas chromatographic (GC) methods. Quantification by dissolution followed by standard addition was also demonstrated to be reliable. Conclusions: The ASAP-TOFMS method is a useful technique for rapidly screening gaskets for the presence of plasticisers. It can be used to identify specific gaskets deserving of further quantitative analysis by chromatographic methods, saving time and money by avoiding unnecessary analyses. Copyright © 2015 John Wiley & Sons, Ltd. Copyright © 2015 John Wiley & Sons, Ltd.

von Goetz N.,ETH Zurich | Pirow R.,Federal Institute for Risk Assessment BfR | Hart A.,Fera Science Ltd FERA | Bradley E.,Fera Science Ltd FERA | And 8 more authors.
Regulatory Toxicology and Pharmacology | Year: 2017

In the most recent risk assessment for Bisphenol A for the first time a multi-route aggregate exposure assessment was conducted by the European Food Safety Authority. This assessment includes exposure via dietary sources, and also contributions of the most important non-dietary sources. Both average and high aggregate exposure were calculated by source-to-dose modeling (forward calculation) for different age groups and compared with estimates based on urinary biomonitoring data (backward calculation). The aggregate exposure estimates obtained by forward and backward modeling are in the same order of magnitude, with forward modeling yielding higher estimates associated with larger uncertainty. Yet, only forward modeling can indicate the relative contribution of different sources. Dietary exposure, especially via canned food, appears to be the most important exposure source and, based on the central aggregate exposure estimates, contributes around 90% to internal exposure to total (conjugated plus unconjugated) BPA. Dermal exposure via thermal paper and to a lesser extent via cosmetic products may contribute around 10% for some age groups. The uncertainty around these estimates is considerable, but since after dermal absorption a first-pass metabolism of BPA by conjugation is lacking, dermal sources may be of equal or even higher toxicological relevance than dietary sources. © 2017 Elsevier Inc.

van Vaerenbergh J.,Belgium Institute for Agricultural and Fisheries Research | Muller P.,Julius Kuhn Institute | Elphinstone J.G.,Fera Science Ltd Fera | Vreeburg R.A.M.,Dutch General Inspection Service | Janse J.D.,Dutch General Inspection Service
EPPO Bulletin | Year: 2017

In the European Union (EU) potato production is surveyed for Clavibacter michiganensis subsp. sepedonicus (potato ring rot) and Ralstonia solanacearum (potato brown rot) under Commission Directives 93/85/EEC with its amendment 2006/56/EC and 98/57/EEC with its amendment 2006/63/EC. A regular update of the Directives is required in view of developments in understanding of the biology of these organisms and the diagnostics recommended for their detection and identification. Three inter-laboratory tests (ILT1, ILT2 and ILT3) were performed from 2009 to 2012 as part of a Euphresco Phytosanitary ERA-NET project to assess performance of current official methods for C. michiganensis subsp. sepedonicus and R. solanacearum. A major aim of the ILTs was to generate data on the performance of real-time PCR protocols to support their introduction as primary (core) screening tests for both pathogens. In ILT1, 29 laboratories from 23 countries participated, in ILT2, 23 laboratories from 18 countries and in ILT3 42 laboratories from 24 countries. Relative accuracies for real-time PCR tests averaged 92% for R. solanacearum and 96% for C. michiganensis subsp. sepedonicus) and compared with existing primary (core) screening tests (immunofluorescence, conventional PCR, semi-selective plating and bioassay) in terms of analytical sensitivity, analytical specificity and robustness. It was concluded that all methods tested, including real-time PCR, can be considered as equivalent. Therefore TaqMan® real-time PCR is recommended for inclusion in EU Directives and EPPO Standards as a reliable primary (core) screening method. © 2016 The Authors. Journal compilation © 2016 OEPP/EPPO

Turner J.,Fera Science Ltd Fera | O'Neill P.,University of Exeter | Grant M.,University of Exeter | Mumford R.A.,Fera Science Ltd Fera | And 2 more authors.
Genomics Data | Year: 2017

Here we present genome sequences for twelve isolates of the invasive pathogen Phytophthora ramorum EU1. The assembled genome sequences and raw sequence data are available via BioProject accession number PRJNA177509. These data will be useful in developing molecular tools for specific detection and identification of this pathogen. © 2017 The Authors

Lammoglia S.-K.,French National Institute for Agricultural Research | Kennedy M.C.,Fera Science Ltd. FERA | Barriuso E.,French National Institute for Agricultural Research | Alletto L.,Purpan Engineering School | And 3 more authors.
Environment International | Year: 2017

Background Reducing the risks and impacts of pesticide use on human health and on the environment is one of the objectives of the European Commission Directive 2009/128/EC in the quest for a sustainable use of pesticides. This Directive, developed through European national plans such as Ecophyto plan in France, promotes the introduction of innovative cropping systems relying, for example, on integrated pest management. Risk assessment for human health of the overall pesticide use in these innovative systems is required before the introduction of those systems to avoid that an innovation becomes a new problem. Objectives The objectives of this work were to assess and to compare (1) the human exposure to pesticides used in conventional and innovative cropping systems designed to reduce pesticide needs, and (2) the corresponding risks for human health. Methods Humans (operator and residents) exposure to pesticides and risks for human health were assessed for each pesticide with the BROWSE model. Then, a method was proposed to represent the overall risk due to all pesticides used in one system. This study considers 3 conventional and 9 associated innovative cropping systems, and 116 plant protection products containing 89 different active substances (i.e. pesticides). Results The modelling results obtained with BROWSE showed that innovative cropping systems such as low input or no herbicide systems would reduce the risk for human health in comparison to the corresponding conventional cropping systems. On the contrary, BROWSE showed that conservation tillage system would lead to unacceptable risks in the conditions of our study, because of a high number of pesticide applications, and especially of some herbicides. For residents, the dermal absorption was the main exposure route while ingestion was found to be negligible. For operators, inhalation was also a predominant route of exposure. In general, human exposure to pesticides and human health risks were found to be correlated to the treatment frequency index TFI (number of registered doses of pesticides used per hectare for one copping season), confirming the relationship between the reduction of pesticide use and the reduction of risks. Conclusions Assessment with the BROWSE model helped to identify cropping systems with decreased risks from pesticides for human health and to propose some improvements to the cropping systems by identifying the pesticides that led to unacceptable risks. © 2017 Elsevier Ltd

Doehl J.S.P.,University of York | Bright Z.,University of York | Bright Z.,University of Exeter | Dey S.,University of York | And 8 more authors.
Nature Communications | Year: 2017

Increasing evidence suggests that the infectiousness of patients for the sand fly vector of visceral leishmaniasis is linked to parasites found in the skin. Using a murine model that supports extensive skin infection with Leishmania donovani, spatial analyses at macro-(quantitative PCR) and micro-(confocal microscopy) scales indicate that parasite distribution is markedly skewed. Mathematical models accounting for this heterogeneity demonstrate that while a patchy distribution reduces the expected number of sand flies acquiring parasites, it increases the infection load for sand flies feeding on a patch, increasing their potential for onward transmission. Models representing patchiness at both macro-A nd micro-scales provide the best fit with experimental sand fly feeding data, pointing to the importance of the skin parasite landscape as a predictor of host infectiousness. Our analysis highlights the skin as a critical site to consider when assessing treatment efficacy, transmission competence and the impact of visceral leishmaniasis elimination campaigns. © 2017 The Author(s).

Bramwell L.,Northumbria University | Glinianaia S.V.,Northumbria University | Rankin J.,Northumbria University | Rose M.,Fera Science Ltd. Fera | And 3 more authors.
Environment International | Year: 2016

The aim of this review was to identify and appraise the current international evidence of associations between concentrations of polybrominated diphenyl ethers (PBDEs) in humans and their indoor dusts and food. We systematically searched Medline, Embase, Web of Science and Scopus (up to Jan 2015), using a comprehensive list of keywords, for English-language studies published in peer-reviewed journals. We extracted information on study design, quality, participants, sample collection methods, adjustments for potential confounders and correlations between PBDE concentrations in internal and external matrices. Of 131 potential articles, 17 studies met the inclusion criteria and were included in the narrative synthesis. We concluded that three key factors influenced correlations between external and internal PBDE exposure; half-life of individual congeners in the human body; proximity and interaction between PBDE source and study subject; and time of study relative to phase out of PBDE technical products. Internal dose of Penta-BDE technical mix congeners generally correlated strongly with dust. The exception was BDE-153 which is known to have higher persistence in human tissues. Despite the low bioaccessibility and short half-life of BDE-209, its high loading in dusts gave strong correlations with body burden where measured. Correlations between PBDE concentrations in duplicate diet and body burden were not apparent from the included studies. Whether dust or diet is the primary exposure source for an individual is tied to the loading of PBDE in dust or food items and the amounts ingested. Simple recommendations such as more frequent hand washing may reduce PBDE body burden. © 2016 Elsevier Ltd.

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