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Cibulski S.P.,FEPAGRO Animal Health Institute Pesquisas Veterinarias Desiderio Finamor IPVDF | Cibulski S.P.,Institute Ciencias Basicas da Saude | Teixeira T.F.,FEPAGRO Animal Health Institute Pesquisas Veterinarias Desiderio Finamor IPVDF | Teixeira T.F.,Institute Ciencias Basicas da Saude | And 10 more authors.
Virus Genes | Year: 2016

A novel bovine parvovirus 2 (BPV2) genotype comprising 5394 nt was identified by next generation sequencing from sera of healthy cattle at different age groups farmed in the state of Rio Grande do Sul, Brazil. The genome organization of new BPV2 genotype retains the two ORFs typical of members of the Parvovirinae with 86.4 % of overall nucleotide sequence identities in comparison to other members of the subfamily. Phylogenetic analysis revealed similar clustering with two previously described bovine BPV2 within the genus Copiparvovirus. No significant differences (P ≥ 0.05) were detected in the distribution of BPV2 infection in cattle at different age groups. This is the third complete or near complete genome sequence of BPV2 reported to date and may contribute to a better understanding of the biology of copiparvoviruses and its interactions with the host. © 2015, Springer Science+Business Media New York. Source


Teixeira T.F.,FEPAGRO Animal Health Institute Pesquisas Veterinarias Desiderio Finamor IPVDF | Teixeira T.F.,Federal University of Rio Grande do Sul | Dezen D.,FEPAGRO Animal Health Institute Pesquisas Veterinarias Desiderio Finamor IPVDF | Cibulski S.P.,FEPAGRO Animal Health Institute Pesquisas Veterinarias Desiderio Finamor IPVDF | And 5 more authors.
PLoS ONE | Year: 2011

Torque teno sus virus (TTSuV), a member of the family Anelloviridae, is a single-stranded, circular DNA virus, widely distributed in swine populations. Presently, two TTSuV genogroups are recognized: Torque teno sus virus 1 (TTSuV1) and Torque teno sus virus 2 (TTSuV2). TTSuV genomes have been found in commercial vaccines for swine, enzyme preparations and other drugs containing components of porcine origin. However, no studies have been made looking for TTSuV in cell cultures. In the present study, a search for TTSuV genomes was carried out in cell culture lineages, in sera used as supplement for cell culture media as well as in trypsin used for cell disaggregation. DNA obtained from twenty-five cell lineages (ten from cultures in routine multiplication and fifteen from frozen ampoules), nine samples of sera used in cell culture media and five batches of trypsin were examined for the presence of TTSuV DNA. Fifteen cell lineages, originated from thirteen different species contained amplifiable TTSuV genomes, including an ampoule with a cell lineage frozen in 1985. Three cell lineages of swine origin were co-infected with both TTSuV1 and TTSuV2. One batch of trypsin contained two distinct TTSuV1 plus one TTSuV2 genome, suggesting that this might have been the source of contamination, as supported by phylogenetic analyses of sequenced amplicons. Samples of fetal bovine and calf sera used in cell culture media did not contain amplifiable TTSuV DNA. This is the first report on the presence of TTSuV as contaminants in cell lineages. In addition, detection of the viral genome in an ampoule frozen in 1985 provides evidence that TTSuV contamination is not a recent event. These findings highlight the risks of TTSuV contamination in cell cultures, what may be source for contamination of biological products or compromise results of studies involving in vitro multiplied cells. © 2011 Teixeira et al. Source


Teixeira T.F.,FEPAGRO Animal Health Institute Pesquisas Veterinarias Desiderio Finamor IPVDF | Teixeira T.F.,Institute Ciencias Basicas da Saude | Cibulski S.P.,FEPAGRO Animal Health Institute Pesquisas Veterinarias Desiderio Finamor IPVDF | Cibulski S.P.,Institute Ciencias Basicas da Saude | And 7 more authors.
Research in Veterinary Science | Year: 2015

Associations between Torque teno sus viruses (TTSuVs) and the occurrence of postweaning multisystemic wasting syndrome (PMWS) have been reported with controversial results. Currently, no studies have been performed comparing simultaneously viral loads of TTSuVs and PCV2. To examine the role for TTSuVs in PMWS-affected animals, a SYBR Green-based quantitative PCR (qPCR) was designed to detect and quantify TTSuV1, TTSuV2 and PCV2 genomes in swine sera. TTSuV1 genome loads were significantly higher in healthy adults than in young and SPF animals (p < 0.05) suggesting that the prevalence of TTSuV1 infection increases with age and bears no association with PMWS. Regarding TTSuV2, no significant variation was detected in viral loads within any of the groups. As expected, PCV2 genome loads were higher in PMWS-affected swine than in healthy or SPF animals (p < 0.001). These findings provide clear evidence to indicate that neither TTSuV1 nor TTSuV2 viral loads have any correlation with the occurrence of PMWS. © 2015 Elsevier B.V. Source

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