Entity

Time filter

Source Type


Ma J.W.,Nanjing Medical University | Qiao Z.Y.,Fengxian Branch of Shanghai 6th Peoples Hospital | Xu B.,Nanjing Medical University
Molecular Biology Reports | Year: 2013

The aim of this study was to characterise the effects of ischemic preconditioning (IP) on heart function parameters (DST andDT), activities of serumcreatine kinase (CK), lactate dehydrogenase (LDH), and levels of serum nitric oxide (NO), malondialdehyde (MDA), and myocardium Caspase-3 mRNA, SOCS-1, SOCS-3, tumor necrosis factor-Alpha (TNF-α) and interleukin-6 (IL-6) expression levels and Apoptosis index in myocardium IR rats. Results showed that DST and DST values in IP group were markedly lower than those in IR group. Compared with IR group, IP significantly (p>0.01) decreased serum CK (0.83 ± 0.09 vs 1.36 ± 0.15), LDH (5613 ± 462 vs 7106 ± 492) activities and MDA (11.32 ± 1.05 vs 15.49 ± 1.26) level, increased the serum NO (86.39 ± 7.03 vs 53.77 ± 4.27) level in IR group. The IP induced a significant decreased in myocardium Caspase-3 mRNA (0.303 ± 0.021 vs 0.515 ± 0.022) gene expression (p>0.01) compared to IR model group. The IP induced a significant decreased in myocardium SOCS-1 (0.241 ± 0.031 vs 0.596 ± 0.036), SOCS-3 (0.258 ± 0.031 vs 0.713 ± 0.057), TNF-A (0.137 ± 0.011 vs 0.427 ± 0.035) and IL-6 (0.314 ± 0.021 vs 0.719 ± 0.064) mRNA gene expression (p>0.01) compared to IR model group. We conclude that IP is effective in the therapy of heart disease. These findings may have implications for the clinical development of preconditioning- based therapies for ischemic heart disease.© Springer Science+Business Media Dordrecht 2013. Source


Ma J.,Nanjing Medical University | Qiao Z.,Fengxian Branch of Shanghai 6th Peoples Hospital | Xu B.,Nanjing Medical University
International Journal of Biological Macromolecules | Year: 2013

In this study, we examined the effects of Prostaglandin E1 and tea polysaccharides (TP) on serum estrogen and FSH levels, myocardium sPLA2-V positive levels, and sPLA2-V protein expression in the rats fed on hypercholesterolemic diet. Hyperlipidemic rats were treated with Prostaglandin E1 and TP. Serum estrogen and FSH levels were significantly enhanced by Prostaglandin E1 and TP, whereas myocardium sPLA2-V positive rate and protein expression levels were decreased compared to the HCD group. Our results suggest that Prostaglandin E1 and TP exert strong heart-protective effects and therefore can be used to reduce the risk of heart disorders. © 2013 Elsevier B.V. Source


Qiao Z.Y.,Fengxian Branch of Shanghai 6th Peoples Hospital | Qiao Z.Y.,Tongji University | Huang J.H.,Fengxian Branch of Shanghai 6th Peoples Hospital | Ma J.W.,Fengxian Branch of Shanghai 6th Peoples Hospital | And 5 more authors.
Molecular Biology Reports | Year: 2014

The Bax, cyt-c and caspase-3 proteins play an important role in regulating the myocardial apoptosis. Although very little is known about the specific signal pathways modulated by Ginkgo biloba extract (GBE), it seems advisable to suppose that GBE-induced antiapoptotic effect might be attributed to the regulation of the expression of these proteins. Our aim was to investigate whether GBE could attenuate ischemia/reperfusion-induced apoptosis in cardiac myocytes and its potential mechanisms. In the myocardium ischemia reperfusion (IR) rat model, treatment of GBE (400 mg/kg) significantly decreased the cardiomyocyte cell apoptosis and myocardium infarction. Immunohistochemical analysis showed that GBE significantly inhibited I/R-induced increase of myocardial Bax, caspase-3, and cyt-c proteins expression. Western blot analysis confirmed results of immunohistochemical analysis. It is most likely that multiple pathways are involved in IR-induced apoptosis in rat myocardium cells. Therefore, these results demonstrate that GBE exhibits significant protective effect against myocardial I/R injury in rat heart, which is related to down-regulate Bax, cyt-c and caspase-3. Bcl-2 overexpression might prevent IR-induced apoptosis by inhibiting cytochrome c release from the mitochondria and block activation of caspase-3. © 2013 Springer Science+Business Media Dordrecht. Source


Liu H.J.,Fengxian Branch of Shanghai 6th Peoples Hospital | Ma J.W.,Fengxian Branch of Shanghai 6th Peoples Hospital | Qiao Z.Y.,Fengxian Branch of Shanghai 6th Peoples Hospital | Xu B.,Nanjing Medical University
Molecular Biology Reports | Year: 2013

Prostaglandin E1 has been used clinically for improving heart diseases. In this study, we examined the effect of Prostaglandin E1 on blood lipid levels, heart protein and genes expression in coronary heart disease (CHD) rats. Female rats were fed either a control diet or hypercholesterolemic diet for 14 weeks. The feeding of a hypercholesterolemic diet (HCD) increased the serum TC, TG, and LDL-c levels, decreased the serum HDL-c, E2, P, FSH, LH and PRL levels in CHD rats. In addition, The feeding of a HCD diet markedly increased the content of serum TXA2, TXB2, and decreased the content of serum PGI2, and PGI2/TXA2, 6-Keto PGF1a. Furthermore, the feeding of a hypercholesterolemic diet markedly increased expression levels of myocardium Fas and Caspase-3 protein and mRNA levels, vascular endothelial growth factor and basic fibroblast growth factor mRNA, and decreased RyR2 mRNA in CHD rats. The feeding of Prostaglandin E1 for 14 weeks significantly reversed these abnormal biochemical indexes in rats. These findings suggest that Prostaglandin E1 play a obvious heart protective effect. The mechanisms may be related to restraining the excessive activation of Fas and Caspase-3 protein and modulating some gene expressions associated with CHD. © 2013 Springer Science+Business Media Dordrecht. Source


Zhang M.,Qingdao University | Zhang M.,Jining Medical University | Cai S.,Qingdao University | Ma J.,Fengxian Branch of Shanghai 6th Peoples Hospital
Gene | Year: 2015

The aim of the present study was to investigate whether soybean oligosaccharides (SO) protects heart function against myocardium ischemia reperfusion (MIR) injury. Hearts were 20. min global ischemia and 50. min reperfusion. Rats were fed for 30. days with saline (sham and MIR groups) or the SO (200 or 400. mg/kg body weight, daily). At the end of 30. days, the left main coronary artery was occluded for 30. min, followed by 24. h reperfusion, in anesthetized rats. Sham operated animals were subjected to the same surgical procedures, except that the suture under the left anterior descending coronary artery was not tied. Results showed that SO decreased malondialdehyde (MDA) level and increased antioxidant enzymes activities in the SO-treatment group. Pre-treated with SO it showed a significant recovery in cardiac contractile function, reduction in infarct size, and decrease in creatine kinase (CK), aspartate transaminase (AST) and lactate dehydrogenase (LDH) activities. Moreover, SO also significantly increased the expression of p-JAK2 and p-STAT3 proteins in rat heart. However, no significant change in JAK2 and STAT3 levels was observed. Activation of JAK2/STAT3 pathway showed a significant protective role in the SO-treatment group. Perhaps, the altered activation of the JAK2/STAT3 pathway in ischemic myocardium is one mechanism by which SO is cardioprotective. © 2014 Elsevier B.V. Source

Discover hidden collaborations