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Min J.Z.,University of Shizuoka | Yamamoto M.,University of Shizuoka | Yu H.-F.,Fengxian Branch | Higashi T.,Tokyo University of Science | Toyo'Oka T.,University of Shizuoka
Analytical Biochemistry | Year: 2012

The resolution of the intermediate advanced glycation end products (AGEs) in the human nail was carried out by the combination of 4,5-dimethyl-1,2- phenylenediamine (DMPD) derivatives and ultra-performance liquid chromatography with electrospray ionization time-of-flight mass spectrometry (UPLC-ESI-TOF-MS). The reaction of the reagent with 3-deoxyglucosone (3-DG), methylglyoxal (MG), and glyoxal (GO) effectively proceeds at 60 °C for 2 h. The resulting derivatives were efficiently separated by a gradient program (a mixture of water and acetonitrile containing 0.1% formic acid) using a reversed-phase ACQUITY UPLC BEH C 18 column (1.7 μm, 50 × 2.1 mm i.d.) and sensitively detected by TOF-MS. The detection limits (signal-to-noise ratio = 5) of the TOF-MS were 10 to 50 fmol. A good linearity was achieved from the calibration curve, which was obtained by plotting the peak area ratios of the analytes relative to the internal standard (IS) (i.e., 2,3-hexanedione) versus the injected amounts of 3-DG, MG, and GO (r 2 > 0.999), and the intra- and interday assay precisions were less than 6.89%. The derivatives of the compounds in the human nail were successfully identified by the proposed procedure. As we know, these three kinds of dicarbonyl intermediates in the formation of AGEs - 3-DG, MG, and GO - were first found in human nail samples. Using these methods, the amounts of compound in the nails of healthy volunteers and diabetic patients were determined. When comparing the index from the diabetic patients with that from healthy volunteers, there is no significant difference in the content of the MG and GO in the nails. However, a statistically significant (P < 0.001) correlation was observed between the 3-DG concentrations. Because the proposed method provides a good mass accuracy and the trace detection of the dicarbonyl intermediates of AGEs in the human nail, this analytical technique could be a noninvasive technique to assist in the diagnosis and assessment of disease activity in diabetic patients. Here we present a novel, sensitive, and simple method for the simultaneous determination of dicarbonyl compounds in the human nail. © 2011 Elsevier Inc. All rights reserved. Source

Meng Q.,Guangdong Academy of Medical science | Zhang S.,Sun Yat Sen University | Cheng H.,Fengxian Branch | Chen X.,Jinan 2nd Peoples Hospital | Jin Y.,Sun Yat Sen University
Graefe's Archive for Clinical and Experimental Ophthalmology | Year: 2010

Background: To evaluate the efficacy and safety of heavy silicone oil Oxane Hd as intraocular tamponade in the treatment of complicated retinal detachment (RD). Methods: Forty eyes of 40 patients with complicated RD were recruited for this prospective study. Inclusion criteria were proliferative vitreoretinopathy grade ≥ CP2, mainly inferior and posterior retinal breaks, or superior retinal breaks with patient's inability to posture. Oxane Hd was used as intraocular tamponade. The retinal status, the best-corrected visual acuity (BCVA) results, and any complications were observed. Results: The mean duration of Oxane Hd endotamponade was 87.9 ± 10.4 days, and the mean follow-up time after Oxane Hd removal was 438.1 ± 153.7 days. The primary anatomical success rate after Oxane Hd removal was 87.5%, and with further intervention 97.5%. The BCVA significantly improved, from mean logMAR 2.12 ± 0.60 to 1.38 ± 0.59 (P < 0.001). The postoperative complications included temporary inflammatory reaction, moderately high intraocular pressure, heavy silicone oil emulsification, lens opacity and retinal proliferative membranes. Conclusions: Without a requirement for postoperative prone position, heavy silicone oil Oxane Hd is effective and safe for the treatment of complicated RD with inferior and posterior breaks. Larger groups and a longer follow-up period will be included to further evaluate the efficacy with Oxane Hd in superior retinal breaks. © 2010 Springer-Verlag. Source

Liu Y.,Shanghai University | He Z.,Fengxian Branch | Feng D.,Shanghai University | Shi G.,Shanghai University | And 4 more authors.
DNA and Cell Biology | Year: 2011

Despite the knowledge of many genetic alterations present in osteosarcoma, the complexity of this disease precludes placing its biology into a simple conceptual framework. Cytotoxic T-lymphocyte antigen-4 (CTLA-4) plays important roles in downregulating T-cell activation, thereby attenuating anti-tumor responses and increasing cancer susceptibility. Polymorphisms in the CTLA-4 gene are associated with different autoimmune diseases and cancers. The current study evaluated the association of four CTLA-4 gene mutations,-1661A/G (rs4553808),-318C/T (rs5742909), +49G/A (rs231775), and CT60A/G (rs3087243), with osteosarcoma in the Chinese population. CTLA-4 polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism in 267 osteosarcoma patients and 282 age-matched healthy controls. Results showed that the CTLA-4 gene +49 AA genotype, +49 A allele, and GTAG haplotype were significantly more frequent in osteosarcoma patients than in controls (odds ratio [OR] 2.20, 95% confidence interval [CI] 1.23-2.95, p = 0.007; OR 1.32, 95% CI 1.03-1.69, p = 0.029, and OR = 1.47, 95% CI 1.03-2.09, p = 0.033, respectively). The CTLA-4 +49G/A polymorphism and GTAG haplotype are associated with increased risk of osteosarcoma. © 2011 Mary Ann Liebert, Inc. Source

Ma J.,Fengxian Branch | Qiao Z.,Fengxian Branch | Xiang X.,Fengxian Branch
Carbohydrate Polymers | Year: 2011

Optimal conditions for the extraction of black fungus polysaccharides were 350 W, 5, 35 min and 90 °C, for ultrasonic power, ratio of water to sample, extraction time and extraction temperature, respectively. Gas chromatography (GC) analysis showed that black fungus polysaccharides contained glucose, xylose, mannose and ribose. Their molar percentages were 6.8%, 34.2%, 50.7% and 8.9%, respectively. FT-IR and NMR analysis showed typical chemical structure of black fungus polysaccharides. In animal experiment, high fat diet feeding for 29 days markedly reduced myocardium and blood antioxidant enzyme activities and enhanced lipid peroxidation level. Administration of black fungus polysaccharides had significantly enhanced myocardium and blood antioxidant enzyme activities and reduced lipid peroxidation level in high fat mice. Our results indicated that black fungus polysaccharides could be beneficial for protection against cardiovascular diseases and its complications. © 2011 Elsevier Ltd. All rights reserved. Source

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