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Negeri Sembilan, Malaysia

Rahmat Z.,University of Technology Malaysia | Ling M.N.,University of Malaysia, Terengganu | Kulaveerasingam H.,University Putra Malaysia | Syed Alwee S.S.R.,Felda Biotechnology Center | Ong Abdullah M.,Malaysian Palm Oil Board
Jurnal Teknologi (Sciences and Engineering) | Year: 2013

The oil palm industry has been affected by abnormality in its clonal palm. Oil palm abnormality which arose from in vitro regeneration was first detected during flowering process. In this study, localized expression of an oil palm homologue of SOC1 gene was investigated using in situ RNA hybridization. Tissue specific localization expression of OPSOC1and OPSOC1-3' showed that SOC1 is expressed in both normal and abnormal flower. The gene is highly expressed in abnormal oil palm flower throughout flower initiation and development. The role of SOC1 in inducing floral organ and its expression pattern provides a better understanding of regulation of OPSOC1 in normal and abnormal oil palm flower. © 2013 Penerbit UTM Press. All rights reserved.


Suranthran P.,University Putra Malaysia | Gantait S.,University Putra Malaysia | Sinniah U.R.,University Putra Malaysia | Subramaniam S.,Universiti Sains Malaysia | And 2 more authors.
Plant Growth Regulation | Year: 2012

The present study evaluates the effect of six loading solutions and five vitrification solutions (VS) and their time of exposure on the survival of oil palm (Elaeis guineensis) polyembryoids in liquid nitrogen (LN). In vitro grown polyembryoids of oil palm were successfully cryopreserved by vitrification with 45% survival. Individual polyembryoids, isolated from 2-month old culture, were precultured in liquid Murashige and Skoog medium supplemented with 0.5 M sucrose for 12 h and treated with a mixture of 10% (w/v) dimethyl sulphoxide (DMSO) plus 0.7 M sucrose for 30 min. Polyembryoids were then subjected to plant vitrification solution-2 (PVS2) (30% (w/v) glycerol plus 15% (w/v) EG plus 15% (w/v) DMSO plus 0.4 M sucrose) exposure for 5 min at 26 ± 2°C and subsequently plunged into LN. Thawed polyembryoids resumed growth within 8 days of culture and shoot development was recorded at 25 days of growth. Scanning electron micrograph revealed that successful regeneration of cryopreserved polyembryoids was due to stabilization of cellular integrity through optimum VS exposure. © 2011 Springer Science+Business Media B.V.


Gantait S.,University Putra Malaysia | Sinniah U.R.,University Putra Malaysia | Suranthran P.,University Putra Malaysia | Suranthran P.,Felda Biotechnology Center | And 2 more authors.
Protoplasma | Year: 2014

In the present study, polyembryoids of oil palm (Elaeis guineensis Jacq.) were cryopreserved with successful revival of 68 % for the first time using the droplet vitrification technique. Excised polyembryoids (3–5-mm diameter) from 3-month-old in vitro cultures were pre-cultured for 12 h in liquid Murashige and Skoog medium supplemented with 0.5 M sucrose. The polyembryoids were osmoprotected in loading solution [10 % (w/v) dimethyl sulphoxide (DMSO) plus 0.7 M sucrose] for 30 min at room temperature and then placed on aluminium strips where they were individually drenched in chilled droplets of vitrification solution (PVS2) [30 % (w/v) glycerol plus 15 % (w/v) ethylene glycol (EG) plus 15 % (w/v) DMSO plus 0.4 M sucrose] for 10 min. The aluminium strips were enclosed in cryovials which were then plunged quickly into liquid nitrogen and kept there for 1 h. The polyembryoids were then thawed and unloaded (using 1.2 M sucrose solution) with subsequent transfer to regeneration medium and stored in zero irradiance. Following for 10 days of storage, polyembryoids were cultured under 16 h photoperiod of 50 μmol m−2 s−1 photosynthetic photon flux density, at 23 ± 1 °C. Post-thaw growth recovery of 68 % was recorded within 2 weeks of culture, and new shoot development was observed at 4 weeks of growth. Scanning electron microscopy revealed that successful regeneration of cryopreserved polyembryoids was related to maintenance of cellular integrity, presumably through PVS2 exposure for 10 min. The present study demonstrated that cryopreservation by droplet vitrification enhanced the regeneration percentages of oil palm in comparison with the conventional vitrification method previously reported. © 2014, Springer-Verlag Wien.


Danial M.,Universiti Sains Malaysia | Keng C.L.,Universiti Sains Malaysia | Alwee S.S.R.S.,Felda Biotechnology Center | Subramaniam S.,Universiti Sains Malaysia
Journal of Medicinal Plants Research | Year: 2011

Seed morphology and histology analysis of Eurycoma longifolia by light microscopes revealed seeds structures of this important medicinal plant at different growing stages. The seed structures of E. longifolia consist of main regions such as epidermis, hypodermis, storage parenchyma and procambium. The cotyledon of E. longifolia develops into a complex and reticulate vascular system. The seed development phases and the development of the vascular system on the progression of germination provide the insight of the actual and accurate information on the E. longifolia cotyledon development period. This information is essential for using the seed as the source of inoculums for the production of the hairy root cultures. Seeds being the storage organ may facilitate the generation of the hairy roots, as it evidently has the essential features like tracheas, which are the main site of infection for Agrobacterium rhizogenes. ©2011 Academic Journals.


Danial M.,Universiti Sains Malaysia | Keng C.L.,Universiti Sains Malaysia | Alwee S.S.R.S.,Felda Biotechnology Center | Mahmood M.,University Putra Malaysia | Subramaniam S.,Universiti Sains Malaysia
Journal of Medicinal Plants Research | Year: 2011

Bacterial chemotaxis is considered the first step in the interaction between motile bacteria and plant cells. Chemotaxis initiates the process of bacterial infection towards the plant cells and thus conferring beneficial attributes to the host. In this study, 5 wild strains and 2 disarmed strains of Agrobacterium rhizogenes were tested for chemotaxis assay using the swarm agar plate method. As expected, strong positive chemotactic response was observed in most of the tested bacteria strains and all the tested strains of Agrobacterium rhizogenes showed positive chemotactic response towards the tested root and somatic embryos of the valuable medicinal plant, Eurycoma longifolia. Therefore, induction of hairy roots is possible in Eurycoma longifolia. Generating hairy roots in Eurycoma longifolia will be highly beneficial mainly to the pharmaceutical industry as this medicinal plant possesses the capacity to produce many secondary metabolites which is proposed to increase sexual virility properties and to have anti cancer properties. © 2011 Academic Journals.

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