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Hangzhou, China

Wen Z.-S.,Zhejiang Ocean University | Liu L.-J.,Zhejiang Ocean University | Qu Y.-L.,Zhejiang Ocean University | OuYang X.-K.,Zhejiang Ocean University | And 2 more authors.
Marine Drugs | Year: 2013

This study was carried out to investigate the protective effects of chitosan nanoparticles (CNP) against hydrogen peroxide (H2O 2)-induced oxidative damage in murine macrophages RAW264.7 cells. After 24 h pre-incubation with CNP (25-200 μg/mL) and chitosan (CS) (50-200 μg/mL, as controls), the viability loss in RAW264.7 cells induced by H 2O2 (500 μM) for 12 h was markedly restored in a concentration-dependent manner as measured by MTT assay (P < 0.05) and decreased in cellular LDH release (P < 0.05). Moreover, CNP also exerted preventive effects on suppressing the production of lipid peroxidation such as malondialdehyde (MDA) (P < 0.05), restoring activities of endogenous antioxidant including superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) (P < 0.05), along with increasing total antioxidant capacity (T-AOC) (P < 0.05). In addition, pre-incubation of CNP with RAW264.7 cells for 24 h resulted in the increase of the gene expression level of endogenous antioxidant enzymes, such as MnSOD and GSH-Px (P < 0.05). At the same concentration, CNP significantly decreased LDH release and MDA (P < 0.05) as well as increased MnSOD, GSH-Px, and T-AOC activities (P < 0.05) as compared to CS. Taken together, our findings suggest that CNP can more effectively protect RAW264.7 cells against oxidative stress by H2O2 as compared to CS, which might be used as a potential natural compound-based antioxidant in the functional food and pharmaceutical industries. © 2013 by the authors. Source

Zheng L.,Zhejiang University | Yuan D.,Zhejiang University | Shi M.-L.,Zhejiang University | Guo X.-Y.,Zhejiang University | And 3 more authors.
Indian Journal of Animal Sciences | Year: 2013

A 50-d experiment using day-old Lingnan Yellow broilers (600) was conducted to investigate effects of light intensity on growth performance and antioxidative status of broilers. The chicks were randomly divided into 4 treatments with light intensity of 5, 10, 20, 30 lx respectively, and each treatment was replicated 3 times. There existed a significant increase in 1-21d feed intake of broilers provided with light intensity of 5 and 10 lx from d 1 to d 21 when compared with those provided with 20 and 30 lx, so did 21-d average body weightÿbut no obvious difference was observed in feed conversion ratio. Higher MET concentration was detected in serum of broilers under lower light intensity. Low light intensity of 5 lx significantly enhanced 21-d total antioxidant capability and activity of glutathione peroxidase both in serum and liver and 50-d GSH-Px activity in serum and reduced maleic dialdehyde in both 50-d serum and liver, 10 lx significantly increased 50-d GSH-Px activity in liver, 5lx and 10lx significantly increased 50-d serum T-AOC, and 20 lx, 10 lx and 5 lx significantly increased 50-d T-AOC and decreased 21-d serum MDA. Low light intensity improved 1-21 d growth performance of broilers and antioxidative status of broilers to some degree, but not overall phase. Thus, low light intensity of 5 or 10 lx are recommended in commercial production of broilers for the sake of producers' profit. Source

Zhang M.,Zhejiang University | Zhang M.,Wilmar Biotechnology Research and Development Center Co. | Zhang A.-T.,Zhejiang University | Zhang A.-T.,Feed Science Institute | And 6 more authors.
Indian Journal of Animal Sciences | Year: 2012

In order to understand the phylogenetic diversity and expression character of prolactin (PRL) molecule, the cDNA of PRL was isolated and cloned from female American silver king pigeon (Columbia livia) pituitary gland by a degenerate primer and nested reverse transcription polymerase chain reaction (RT-PCR) based strategy. The expected expression of mRNA in different tissues and status was quantified by real-time quantitative PCR (FQ-PCR). The cloned cDNA contains 658bp nucleotides, encoded 219 amino acids and deduced amino acid sequences showed high similarities and identity with the corresponding sequences of chicken, budgerigar, India pealfowl, comb duck, duck, goose, turkey, ring-necked pheasant, Java sparrow, ostrich, ring-necked pheasant and quail. PRL was widely expressed in tissues analyzed, and highest level of PRL mRNA expression was observed in the pitutary whereas the lowest in the kidney. Comparable high level of PRL mRNA expression was observed in oviduct and ovary. PRL mRNA expression increased significantly in incubation, compared to laying. Source

Wang Y.-Z.,Feed Science Institute | Huang Y.-N.,Feed Science Institute | Sun K.-Y.,Zhejiang University | Qi J.-H.,Zhejiang University | Xiang L.,Zhejiang University
International Journal of Molecular Medicine | Year: 2011

Leptin gene transfer in the liver by hydrodynamic-based gene delivery instead of peptide administration was used to investigate the effects of leptin on muscle mass accretion and lipid accumulation in muscles of wild-type mice. Food intake (P<0.01), body weight (P<0.01) and white adipose tissue (P<0.01) were significantly reduced in the leptin gene-treated group compared with the control group. Moreover, plasma leptin concentration was significantly increased after administration of the mouse leptin gene at a dose of 15 μg per mouse for 1 day (P<0.01) or 1 week (P<0.05). Furthermore, the mRNA abundance of myosin heavy chain type I (MyHC-I), myosin heavy chain type II (MyHC-IIa, MyHC-IIx), adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL) and lipoprotein lipase (LPL) genes in gastrocnemius muscle and extensor digitorum longus after administration of leptin for 1 week were significantly increased compared with the control group. Finally, we investigated the mechanism by which leptin gene transfer affects fibromuscular and fat deposition in muscle. Gene expression and protein levels of SIRT1, and proliferator-activated receptor-γ coactivator-1α (PGC-1α) were remarkably increased in extensor digitorum longus. On the other hand, PGC-1α and FOXO3a gene expression was observed to have significantly increased in gastrocnemius muscle. However, only changes in the protein levels of PGC-1α were observed (P<0.05). These results suggest that leptin may affect the growth and development of muscle, and fat deposition in wild-type mice via SIRT1 and FOXO3a and their downstream targets, including PGC-1α. Source

Dai W.,Tianjin Agricultural University | Dai W.,Feed Science Institute | Du H.,Feed Science Institute | Fu L.,Zhejiang GongShang University | And 2 more authors.
Applied Clay Science | Year: 2010

The effect of montmorillonite (MMT) on dietary lead (Pb) accumulation in tissues of tilapia (Oreochromis niloticus) was investigated. Two hundred forty tilapia were randomly divided into four groups and fed with a basal diet, a basal diet supplemented with 0.5% MMT, Pb-contaminated basal diet (100 mg Pb/kg dry mass) and Pb-contaminated basal diet supplemented with 0.5% MMT, respectively. After 60 days, tilapia were sacrificed and sampled to determine Pb accumulation, zinc and iron concentration in the tissues of tilapia. MMT significantly reduced Pb accumulation in kidney, intestine, bone, stomach, gill, liver, spleen, muscle, spermary and brain of tilapia(P < 0.05). Concentration of zinc (Zn) and iron (Fe) in the sampled tissues (kidney, bone, liver, spleen and spermary) of tilapia were not affected by MMT (P > 0.05). Addition of MMT in diet could protect tilapia from dietary Pb accumulation in tissues. © 2009. Source

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