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Cinotti E.,Jean Monnet University | Perrot J.L.,Jean Monnet University | Labeille B.,Jean Monnet University | Espinasse M.,Jean Monnet University | And 11 more authors.
Journal of Biomedical Optics | Year: 2013

Nephropathic cystinosis (NC) is a rare autosomal recessive storage disease characterized by the lysosomal accumulation of cystine crystals throughout the body, particularly in blood cells, the cornea, skin, kidneys, the central nervous system, and the muscles. The skin and the cornea are the most accessible sites to explore, and in vivo reflectance confocal microscopy (IVCM) helps identify crystals in both but does not provide any information to help define their composition. Raman spectroscopy (RS) allows cystine to be easily recognized thanks to its characteristic signature with a band at 499 cm -1. Two dermatology confocal microscopes were used to visualize crystals in both the skin and the ocular surface of a cystinosis patient, and an ex vivo Raman examination of a skin biopsy and of the cornea was performed and removed during a corneal graft to confirm the cystine composition of the crystals. Recently, RS has been performed in vivo and coupled with IVCM. In the future, it is suggested that crystals in NC and other deposits in storage diseases could be identified with this noninvasive in vivo technique that combines IVCM to recognize the deposits and RS to confirm their chemical nature. © 2013 Society of Photo-Optical Instrumentation Engineers.

Jullienne R.,Jean Monnet University | He Z.,Federative Institute of Research in science and Health Engineering | Manoli P.,Jean Monnet University | Grivet D.,Jean Monnet University | And 9 more authors.
Cornea | Year: 2015

Purpose: Multimodal imaging of processionary caterpillar hair-induced keratitis with anterior segment optical coherence tomography and in vivo confocal microscopy. Methods: Case report. Results: A 25-year-old woman presented with acute keratitis induced by multiple tiny processionary caterpillar hairs. She initially experienced severe pain and moderate vision loss, which gradually improved within a few weeks. Diagnosis was confirmed by in vivo confocal microscopy showing a pathognomonic image strictly comparable with ex vivo microscopy photography. Conclusions: To the best of our knowledge, this is the first case of corneal in vivo confocal imaging of a caterpillar hair with confirmation by ex vivo microscopy. Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved.

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