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Kirilina E.A.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Masternak T.B.,Federal Medicobiological Agency of Russia | Efremov M.A.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Kamyshnikov O.Yu.,Federal Medicobiological Agency of Russia
Russian Journal of Bioorganic Chemistry | Year: 2013

The capacity of the bone marrow-derived myelopeptide-1 (MP-1) to affect in vivo and in vitro the functional activity of phagocytes of intact mice and mice treated with a cytostatic agent (cyclophosphane) has been studied. It was found that MP-1 produces a correcting effect on the functional activity of bone marrow and peripheral blood phagocytes. An optimal scheme of the injection of MP-1 to mice with the cyclophosphane-induced immunodeficiency was developed, which provides a maximum immunocorrecting action. MP-1 had the most pronounced effect on the quantitative characteristics and the functional activity of phagocytes of different localization when introduced prior to the cytostatic; under these conditions, the pep tide affects peripheral blood neutrophils. The results obtained enable one to consider MP-1 as a preparation protecting the peripheral blood phagocytes from the damaging action of cyclophosphane. © 2013 Pleiades Publishing, Ltd.


Rohde G.,Imperial College London | Rohde G.,Maastricht University | Message S.D.,Imperial College London | Haas J.J.,Imperial College London | And 10 more authors.
Clinical and Experimental Allergy | Year: 2014

Rationale: Rhinoviruses (RVs) are the major triggers of asthma exacerbations. We have shown previously that lower respiratory tract symptoms, airflow obstruction, and neutrophilic airway inflammation were increased in experimental RV-induced asthma exacerbations. Objectives: We hypothesized that neutrophil-related CXC chemokines and antimicrobial peptides are increased and related to clinical, virologic, and pathologic outcomes in RV-induced exacerbations of asthma. Methods: Protein levels of antimicrobial peptides (SLPI, HNP 1-3, elafin, and LL-37) and neutrophil chemokines (CXCL1/GRO-α, CXCL2/GRO-β, CXCL5/ENA-78, CXCL6/GCP-2, CXCL7/NAP-2, and CXCL8/IL-8) were determined in bronchoalveolar lavage (BAL) fluid of 10 asthmatics and 15 normal controls taken before, at day four during and 6 weeks post-experimental infection. Results: BAL HNP 1-3 and Elafin were higher, CXCL7/NAP-2 was lower in asthmatics compared with controls at day 4 (P = 0.035, P = 0.048, and P = 0.025, respectively). BAL HNP 1-3 and CXCL8/IL-8 were increased during infection (P = 0.003 and P = 0.011, respectively). There was a trend to increased BAL neutrophils at day 4 compared with baseline (P = 0.076). BAL HNP 1-3 was positively correlated with BAL neutrophil numbers at day 4. There were no correlations between clinical parameters and HNP1-3 or IL-8 levels. Conclusions: We propose that RV infection in asthma leads to increased release of CXCL8/IL-8, attracting neutrophils into the airways where they release HNP 1-3, which further enhances airway neutrophilia. Strategies to inhibit CXCL8/IL-8 may be useful in treatment of virus-induced asthma exacerbations. © 2014 John Wiley & Sons Ltd.


Zagainov V.E.,Nizhny Novgorod State Medical Academy | Kostrov A.V.,RAS Institute of Applied Physics | Strikovsky A.V.,RAS Institute of Applied Physics | Gorokhov G.G.,The Surgical Center | And 6 more authors.
Sovremennye Tehnologii v Medicine | Year: 2011

A method of the liver tumor destruction is elaborated according to experimental investigations of a domestic device. It is used for a contact thermal destruction of tumors by the SHF energy local effect and applied in the clinic. The method of the tumor hyperthermia by a local SHF-effect has demonstrated a high effectiveness due to a short period of effect and the ability program the form and the size of the destruction area. Specific zones of the tumor tissue destruction are revealed.


Maruk A.Y.,Federal Medicobiological Agency of Russia | Bruskin A.B.,Federal Medicobiological Agency of Russia | Kodina G.E.,Federal Medicobiological Agency of Russia
Radiochemistry | Year: 2011

Today the main radionuclide used for preparing radiopharmaceuticals throughout the world is 99m Tc, thanks to its optimal nuclear-physical characteristics and ready availability. Several approaches are used for tethering this radionuclide to biomolecules (peptides, antibodies, etc.); one of them involves the use of so-called bifunctional chelating agents (BCAs). These compounds are capable both of binding 99m Tc and of linking to biomolecules. Today the most frequently used BCAs are DTPA, MAG3, and HYNIC. These BCAs are described and compared. The peptides that are most frequently used in combination with these agents are described and compared. Published data on the choice of a coligand for use in combination with HYNIC are discussed. © 2011 Pleiades Publishing, Ltd.


Dmitrieva O.S.,Fox Chase Cancer Center | Dmitrieva O.S.,Federal Medicobiological Agency of Russia | Shilovskiy I.P.,Federal Medicobiological Agency of Russia | Khaitov M.R.,Federal Medicobiological Agency of Russia | And 2 more authors.
Biochemistry (Moscow) | Year: 2016

The idea of a potential link between cancer and inflammation was first proposed by R. Virchow in the nineteenth century. However, clear evidence regarding a key role of inflammation in oncogenesis appeared only during the last decade. Now the tumor microenvironment is commonly considered as an obligatory and significant component of almost all types of cancer, and the cells infiltrating such microenvironment are a source of inflammatory cytokines. Such cytokines play a key role in regulating inflammation during both normal immune response and developing cancer. In this review, we explore the role of two inflammatory cytokines interleukin 1 and interleukin 6 in cancer development. These cytokines have pleiotropic effects on various cell types in the tumor microenvironment, particularly being able to regulate pro-oncogenic transcription factors NF-κB and STAT3. For this reason, such cytokines influence key parameters of oncogenesis, increasing cell resistance to apoptosis, proliferation of cancer cells, angiogenesis, invasion and malignancy as well as the ability of tumor cells to respond to anticancer therapy. Here we summarize novel experimental data regarding mechanisms underlying the interaction between chronic inflammation and malignant neoplasms. © 2016 Pleiades Publishing, Ltd.

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