Federal Medico Biological Agency of Russia

Moscow, Russia

Federal Medico Biological Agency of Russia

Moscow, Russia
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Kuzovlev A.N.,V A Negovsky Research Institute Of General Reanimatology | Abdusalamov S.N.,Federal Medico Biological Agency of Russia | Kuz'michev K.A.,Moscow Clinical Theoretical and Practical Center
Obshchaya Reanimatologiya | Year: 2016

The survival of patients after the sudden circulatory arrest (SCA) depends not only on immediate onset of resuscitative measures, but also on their quality. The purpose of the study. The purpose is to assess the compliance of basic and expanded resuscitative measures carried out by healthcare providers in hospitals with modern national and international guidelines within the frames of a stimulation course. Materials and Methods. The research was perfomed in a multifield hospital in Moscow, in 2016. It consisted of two phases. During the first phase, within the frames of a simulation course, providers’ skills in the cardiopulmonary resuscitation (CPR) and chest compression (CC) technique mastership were evaluated. During the sec_ ond stage, their skills in expanded CPR and ability to work as a part of resuscitation teams were assessed. During the simulation, all team activities were recorded (both audio and video); CC parameters were also registered using a CC pressure control sensor (hereinafter referred to as a sensor) and audiovisual tips. The European Resuscitation Council Guidelines for Resuscitation 2015 were used as reference criteria. The analysis was performed using the ZOLL RescueNet Code Review® software. A statistical analysis was performed using the Statistica 7.0 software (Mann-Whitney U-test). The data were presented as a mean, median ± 25—75 percentiles (25—75 IQR), minimum and maximum values. The difference was considered significant at P<0.05. Results. Test results of most healthcare providers were unsatisfactory when the CPR was performed without sensors and audiovisual tips: the percentage of target CCs was not more than 10% in 72% of providers (n=18). When the CPR was performed with sensors and audiovisual tips regulating the CC quality, the percentage of target CCs was 65.7%. i.e. it was significantly higher than that during the CPR without the sensor and the tips (P=0.0000). While only one provider was able to perform the target CC without the sensor and the tips (4%), 12 providers were able to do it with the sensor (48%) (P=0.0000). In all resuscitation teams, there was a lack in compliance with the ECR 2015 guidelines for expanded CPR, as well as ineffective team work was revealed. Chest compressions did not comply with recommended parameters; pauses before and after defibrillator discharge were too long. In most cases, there was hyperventilation during the artificial lung ventilation. The safety principle was not followed by one of resuscitation teams during the defibrillation procedure. Conclusion. The obtained data demonstrate that healthcare providers have poor skills in basic and expanded CPR. Therefore, it is important to train and retrain healthcare providers in basic and expanded CPR within the frames of simulation training courses on a regular basis (in accordance with European Resuscitation Council Guidelines for Resuscitation 2015 and National Resuscitation Council). During training, the use of technical means of monitoring of the chest compression quality control in CPR should be warranted. It is important to arrange regular retraining in order to keep the skills uptodate, as well as regular debriefings on the CPR quality after each case of resuscitation measures in a hospital. © 2016, V.A. Negovsky Research Institute of General Reanimatology. All rights reserved.

Zastrozhin M.S.,Peoples' Friendship University of Russia | Smirnov V.V.,Federal Medico Biological Agency of Russia | Sychev D.A.,Moscow Medical Academy | Savchenko L.M.,Moscow Medical Academy | And 5 more authors.
Eksperimental'naya i Klinicheskaya Farmakologiya | Year: 2016

According to literature data, isoenzyme CYP3A4 of cytochrome P450 is involved in biotransformation of drugs. At the same time, there is evidence that carbamazepine induces CYP3A4 activity. The purpose of the study was to evaluate the effect of carbamazepine on the CYP3A4 activity in patients with alcohol addiction. The study was performed on a group of 25 men with alcohol abuse, which received haloperidol during the exacerbation of addiction. The activity of CYP23A4 was evaluated using high performance liquid chromatography with mass spectrometry (HPLC-MS/MS) for determining 6-beta-hydroxycortisoI conversion to Cortisol in urine. The results were used to construct a plot and derive an equation of logarithmic regression reflecting the dependence of CYP3A4 activity on the dose of carbamazepine: y = (5.5-9.1) × 10-5 ΔΔx2. These data demonstrate a statistically significant effect of carbamazepine on the activity of CYP3A4 isoenzyme in patients with alcohol addiction treated by haloperidol.

Bagaev A.V.,Federal medico biological Agency of Russia | Pichugin A.V.,Federal medico biological Agency of Russia | Lebedeva E.S.,Federal medico biological Agency of Russia | Ataullakhanov R.I.,Federal medico biological Agency of Russia | Khaitov R.M.,Federal medico biological Agency of Russia
Immunologiya | Year: 2015

Replication-defective recombinant adenoviral vectors (rAd) represent one of the advantageous platforms for development of genetic vaccines. A target gene inserted into rAd is transcribed in cells of the vaccinated human, namely in macrophages and dendritic cells which are effective antigen-presenting cells (APC). The target antigen production is continued in the human organism during 2-3 weeks following administration of rAd, encoding the antigen. It induces intensive responses of T- And B-cells specific to the target antigen. Immune responses against the rAd-encoded target antigen can be enhanced with a use of immune adjuvants, in particular, agonists of Toll-like receptors which stimulate APC for production of pro-inflammatory cytokines and chemokines as well as expression of co-stimulatory receptors on the cell membrane. We hypothesized that a combined use o f TLR-agonists with the target antigen encoding rAd can enhance a production of the target antigen in APC, along with above mentioned immunostimulation effects. In this work we showed an immunoadjuvant effect of TLR4-agonist (Immunomax) administered in BALB/c mice during their immunization with the rAd-HA construct comprising gene of H1N1 Influenza virus hemagglutinin. Using in vitro cell cultures a direct stimulation of antigen-presenting dendritic cells by the TLR4-agonist was revealed which significantly enhanced the response of interferon-gamma secreting antigen-recognizing T cells. We examined expression of rAd-encoded protein antigens in dendritic cells and macrophages. Using cytoplasmic, membrane-bound and secretory target proteins, we showed that TLR4-agonist enhanced production of these three target proteins in APC. Both the number of APCs producing the antigen and the accumulation of the target protein in each antigen-producing cell were increased under influence of the TLR4-agonist. A comparison of Immunomax with agonists of other TLRs revealed that agonists of TLR2, TLR4, TLR5, TLR7, TLR8 and TLR9 enhanced, but the agonist of TLR3, unexpectedly, suppressed the expression of rAd-encoded target proteins.

Zhurkovich I.K.,Federal Medico Biological Agency of Russia | Rudenko A.O.,Federal Medico Biological Agency of Russia | Chelovechkova V.V.,Federal Medico Biological Agency of Russia | Merkusheva I.A.,Federal Medico Biological Agency of Russia | And 5 more authors.
Pharmaceutical Chemistry Journal | Year: 2015

A method for determining buprenorphine and naloxone in human plasma using liquid chromatography and high-resolution time-of-flight mass spectrometry (LC-MS Q-TOF) was developed. The sample preparation technique included liquid extraction with salting out of the analytes in Toxi-Tubes Aintended for the determination of neutral and basic compounds. Quantitative analysis was carried out by the internal standard method. Naltrexone, which was structurally similar to the analytes, was used as the internal standard. The proposed method was developed for clinical trials of the new drug bupraxone, a sublingual dosage form containing buprenorphine and naloxone at doses of 0.2 mg each. The bioavailability of the drug components upon sublingual administration was studied. The buprenorphine concentrations in human plasma after administration at a dose of 0.4 mg via two routes (intravenous and sublingual) were compared. © 2015, Springer Science+Business Media New York.

Nikitin A.G.,Federal Medico Biological Agency of Russia | Potapov V.A.,Arkhimed Clinics of New Medical Technologies | Brovkin A.N.,Federal Medico Biological Agency of Russia | Lavrikova E.Y.,Federal Medico Biological Agency of Russia | And 7 more authors.
Molecular Biology | Year: 2015

To test FTO, KCNJ11, SLC30A8, and CDKN2B for association with type 2 diabetes mellitus (DM), the allele and genotype frequency distributions were established for several polymorphisms of the genes. A comparison of allele and genotype frequencies between DM patients and healthy subjects implicated CKNJ11, SLC30A8, and CDKN2B, but not FTO, in the disease. The genes that affect insulin production and secretion in pancreatic β cells proved to play a main role in type 2 DM in the Russian population. © 2015, Pleiades Publishing, Inc.

Panasenko O.M.,Russian National Research Medical University | Mikhalchik E.V.,Federal Medico Biological Agency of Russia | Gorudko I.V.,Belarussian State University | Grigorieva D.V.,Belarussian State University | And 4 more authors.
Biophysics (Russian Federation) | Year: 2016

Hypochlorous acid-modified human blood low density lipoprotein (LDL–HOCl) was shown to stimulate neutrophils and to increase the luminol- (lm-CL) or lucigenin-enhanced chemiluminescence (lc-CL) of neutrophils. Antioxidants and HOCl scavengers (glutathione, taurine, cysteine, methionine, ceruloplasmin, and human serum albumin (HSA)) were tested for effects on lm-CL, lc-CL, H2O2 production, and degranulation of azurophilic granules of neutrophils. All agents used in increasing concentrations were found to decrease lm-CL produced by neutrophils upon stimulation with LDL–HOCl or subsequent treatment with the activator phorbol 12-myristate 13-acetate (PMA). The agents exerted a far lower, if any, effect on lc-CL and the H2O2 production by neutrophils in the same conditions. In the majority of cases, a decline in neutrophil chemiluminescence in the presence of the agents was not related to their effect on neutrophil degranulation, but was most likely due to their direct interactions with reactive halogen (RHS) or oxygen (ROS) species generated upon neutrophil activation or to myeloperoxidase (MPO) inhibition. Antioxidants and HOCl scavengers present in the human body were assumed to decelerate the development of oxidative or halogenative stress and thereby prevent neutrophil activation. © 2016, Pleiades Publishing, Inc.

Ivanenko N.B.,Saint Petersburg State University | Solovyev N.D.,Saint Petersburg State University | Ivanenko A.A.,Federal Medico Biological Agency of Russia | Ganeev A.A.,Saint Petersburg State University
Archives of Environmental Contamination and Toxicology | Year: 2012

Determination of aluminum (Al), beryllium (Be), cadmium (Cd), chromium (Cr), mercury (Hg), manganese (Mn), nickel (Ni), lead (Pb), and thallium (Tl) concentrations in human blood using high-frequency modulation polarization Zeeman graphite furnace atomic absorption spectrometry (GFAAS) was performed. No sample digestion was used in the current study. Blood samples were diluted with deionized water or 0.1 % (m/v) Triton X-100 solution for Tl. Dilution factors ranged from 1/5 per volume for Be and Tl to 1/20 per volume for Cd and Pb. For Tl, Cd, and Hg, noble metals (gold, platinum, rhodium, etc.) were applied as surface modifiers. To mitigate chloride interference, 2 % (m/v) solution of NH4NO3 was used as matrix modifier for Tl and Ni assessment. The use of Pd(NO3)2 as oxidative modifier was necessary for blood Hg and Tl measurement. Validation of the methods was performed by analyzing two-level reference material Seronorm. The precision of the designed methods as relative SD was between 4 and 12 % (middle of a dynamic range) depending on the element. For additional validation, spiked blood samples were analyzed. Limits of detection (LoDs, 3σ, n = 10) for undiluted blood samples were 2.0 μg L-1 for Al, 0.08 μg L-1 for Be, 0.10 μg L-1 for Cd, 2.2 μg L-1 for Cr, 7 μg L-1 for Hg, 0.4 7mu;g L-1 for Mn, 2.3 lg L-1 for Ni, 3.4 μg L-1 for Pb, and 0.5 μg L-1 for Tl. The LoDs achieved allowed determination of Al, Cd, Cr, Mn, Ni, and Pb at both toxic and background levels. Be, Hg, and Tl could be reliably measured at toxic levels only. The methods developed are used for clinical diagnostics and biological monitoring of work-related exposure. © Springer Science+Business Media, LLC 2012.

Zaytsev K.I.,Moscow State Technical University | Kudrin K.G.,Federal Medico Biological Agency of Russia | Karasik V.E.,Moscow State Technical University | Reshetov I.V.,Federal Medico Biological Agency of Russia | Yurchenko S.O.,Moscow State Technical University
Applied Physics Letters | Year: 2015

In vivo terahertz (THz) spectroscopy of pigmentary skin nevi is performed. The in vivo THz dielectric characteristics of healthy skin and dysplastic and non-dysplastic skin nevi are reconstructed and analyzed. The dielectric permittivity curves of these samples in the THz range exhibit significant differences that could allow non-invasive early diagnosis of dysplastic nevi, which are melanoma precursors. An approach for differentiating dysplastic and non-dysplastic skin nevi using the THz dielectric permittivity is proposed. The results demonstrate that THz pulsed spectroscopy is potentially an effective tool for non-invasive early diagnosis of dysplastic nevi and melanomas of the skin. © 2015 AIP Publishing LLC.

Markova T.P.,Federal Medico Biological Agency of Russia
Voprosy Prakticheskoi Pediatrii | Year: 2013

The problem of frequent recurrent acute respiratory diseases (ARD) in children is highly topical due to the possibility of development of chronic diseases of the respiratory organs and high social significance. Persistence of bacterial flora, decrease of its sensitivity to antibiotics, risk for development of complications set the topical tasks of looking for alternative approaches to treatment and prevention of respiratory infections. In this connection, bacterial immunomodulators are of special interest. Based on the composition and specific features of the mechanism of action, they can be divided into two groups: bacterial immunomodulators with systemic action and bacterial immunomodulators with predominantly topical action. The lecture sums up the experience of using the systemic bacterial immunomodulator Broncho-Vaxom in frequently ill children. As is shown, administration of the drug both in the acute period of respiratory infection and for preventive purposes leads to reduction of the duration of disease, its milder course, decreases the need for administration of symptomatic drugs and also the number of recurrent episodes of ARD.

Bekhtereva M.K.,Federal Medico Biological Agency of Russia | Luppova N.E.,North State Medical University | Privorotskiy V.F.,North State Medical University
Voprosy Prakticheskoi Pediatrii | Year: 2014

The review presents current literature data about traveller's diarrhoeas. This problem becomes more and more topical due to the increasing number of travellers throughout the world, including Russia. The «geography» of such trips broadens every year. People's movement from habitual residences to novel places (often exotic countries) inevitably changes nutrition stereotypes, water ration, not mentioning hygienic specificities. The infectious factor, whose influence determines the character of diarrhoea, its severity and duration, becomes especially important. The work deals with the best studied aspects of the problem of traveller's diarrhoea, such as epidemiology, etiological factors, pathogenetic variants, prevention and treatment options.

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