Federal Medico Biological Agency

Seversk, Russia

Federal Medico Biological Agency

Seversk, Russia

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Drobyshev E.J.,Institute of Toxicology of Federal Medico Biological Agency | Solovyev N.D.,Saint Petersburg State University | Ivanenko N.B.,Saint Petersburg State University | Kombarova M.Y.,Federal Medico Biological Agency | Ganeev A.A.,Saint Petersburg State University
Journal of Trace Elements in Medicine and Biology | Year: 2017

In the current study, a biomonitoring of 18 hair trace elements (Al, As, Cd, Co, Cr, Cu, Fe, Hg, Mg, Mn, Ni, Pb, Se, V, Zn, Ca, Na and P) in school children from Leningradskaya Oblast’ is reported. A case group, residing in a proximity to the toxic waste disposal grounds (Krasniy Bor), has been assessed vs. controls from a non-urban settlement Seltso. In total, 166 hair samples were analysed using double focusing sector field inductively coupled plasma mass spectrometry after microwave-assisted sample digestion with nitric acid. For the determination of Ca, Na and P inductively coupled plasma optical emission spectrometry was employed. For the validation, a reference material and spiked hair samples were analysed. The data obtained was processed using parametric statistics and factor analysis. Determined concentrations of trace elements were in agreement with the previously published results on chemically polluted areas. In the case group, linear correlations between Al, Cr, Cu, Fe, Ni and V were observed. Also, these metals correlated to selenium hair content in the case group. Additionally, a correlation between hair Se and P was observed in the case subjects. Several gender differences in trace content were observed within each group. However, no age- or body index-related difference was found. The obtained results show that closely located waste disposal grounds intensifies trace element exposure in school children of Krasniy Bor. However, judging from rather high values for the controls, total environmental status of the region seems to be unstable, so additional monitoring and chemical safety measures are required. © 2016 Elsevier GmbH


Ivanenko N.B.,Saint Petersburg State University | Ivanenko A.A.,Federal Medico Biological Agency | Solovyev N.D.,Saint Petersburg State University | Zeimal A.E.,Saint Petersburg State University | And 2 more authors.
Talanta | Year: 2013

A sector field inductively coupled plasma mass spectrometry method for the determination of Ag, Al, As, Ba, Be, Cd, Co, Cr, Cs, Cu, Fe, Mn, Ni, Pb, Se, Sr, Tl, U, V and Zn in whole blood and urine was designed. Microwave-assisted digestion with concentrated nitric acid was used for blood samples. Urine samples were analyzed after 1/50 (v/v) dilution with 5% (v/v) nitric acid. For beryllium the necessity of medium resolution mode (R=4000) was shown. Method validation was performed using blood and urine reference materials and by analyzing of spiked samples. For the designed method relative standard deviation (RSD) for the concentration range 0.01-1.0 μg/L was 5-10%. RSD did not exceed 3% when trace elements concentrations were above 1.0 μg/L. Method detection limits (3σ): Ag 0.7 ng/L, Al 16 ng/L, As 3.4 ng/L, Ba 0.02 ng/L, Be 1.5 ng/L, Cd 7.7 ng/L, Co 1.0 ng/L, Cr 2.8 ng/L, Cs 9.8 ng/L, Cu 27 ng/L, Fe 1.1 ng/L, Mn 1.8 ng/L, Ni 17 ng/L, Pb 13 ng/L, Se 0.07 ng/L, Sr 5.7 ng/L, Tl 0.2 ng/L, U 0.1 ng/L, V 0.7 ng/L and Zn 1.2 ng/L. A developed method was applied for trace element biomonitoring of occupationally exposed workers of a beryllium processing enterprise. For preliminary risk assessment technological surface dust had been analyzed by inductively coupled plasma optical emission spectrometry. Based upon results of 50 blood and 40 urine samples analyses occupational exposure evaluation was performed. Exposure risks were found not to exceed acceptable ranges. Possible health hazards were found for Be and also Al, Cr, Mn. Occupational health and safety recommendations for the biomonitored enterprise medical care unit were issued as a result of the current investigation. © 2013 Elsevier B.V. All rights reserved.


PubMed | Federal Medico Biological Agency, Orenburg State University, Center for Biotic Medicine and Institute of General Pathology and Pathophysiology
Type: Journal Article | Journal: Acta scientiarum polonorum. Technologia alimentaria | Year: 2017

A significant association between Zn and Se homeostasis exists. At the same time, data on the influence of zinc supplementation on selenium distribution in organs and tissues seem to be absent. Therefore, the primary objective of the current study is to investigate the influence of zinc asparaginate supplementation on zinc and selenium distribution and serum superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity in Wistar rats.36 rats were used in the experiment. The duration of the experiment was 7 and 14 days in the first and second series, respectively. The rats in Group I were used as the control ones. Animals in Groups II and III daily obtained zinc asparaginate (ZnA) in the doses of 5 and 15 mg/kg weight, respectively. Zinc and selenium content in liver, kidneys, heart, muscle, serum and hair was assessed using inductively coupled plasma mass spectrometry. Serum SOD and GPx activity was analysed spectrophotometrically using Randox kits.Intragastric administration of zinc asparaginate significantly increased liver, kidney, and serum zinc content without affecting skeletal and cardiac muscle levels. Zinc supplementation also stimulated selenium retention in the rats organs. Moreover, a significant positive correlation between zinc and selenium content was observed. Finally, zinc asparaginate treatment has been shown to modulate serum GPx but not SOD activity.The obtained data indicate that zinc-induced increase in GPx activity may be mediated through modulation of selenium status. However, future studies are required to estimate the exact mechanisms of zinc and selenium interplay.


PubMed | Federal Medico Biological Agency, Peoples' Friendship University of Russia and Moscow Medical Academy
Type: Journal Article | Journal: Voprosy kurortologii, fizioterapii, i lechebnoi fizicheskoi kultury | Year: 2016

The flexors/extensors imbalance can be a cause knee instability and therefore lead to progression of osteoarthritis. The isokinetic evaluation of the strength of flexors after the autoplastic reconstruction the anterior cross-shaped (cruciate) ligament (ACL) is of primary importance bearing in mind that certain authors have reported a persistent deficiency in flexor strength after surgery.The purpose of the present study was to objectively evaluate the isokinetic function of the knee during each treatment stage and estimate the influence of harvesting semitendinosus/gracilis tendon (SGT) on the knee flexion.During the period from 2012 to 2014, a total of 44 patients (20 women and 24 men) presenting with ACL rupture were available for the examination. All the patients underwent the isokinetic evaluation of the knee function before operation, 6-8 weeks and 3-4 months after the arthroscopic ACL reconstruction with the use of the SGT graft. The patients were divided into two groups. The first group was comprised of 22 subjects who were treated in accordance with the standard protocol of therapeutic physical exercises starting from week 6 after the initiation of the physiotherapeutic rehabilitative measures. The second group consisted of 22 patients who had to perform the isokinetic training within 6-8 weeks after surgery.The results of the study indicate that all the patients suffering injuries to the anterior cross-shaped ligaments had well apparent flexor/extensor imbalance. In those of the first group, the flexor imbalance amounted to 25-35% while the extensor imbalance was 10-15% within 3-4 months after the surgery. The second group showed a 10-15% flexor imbalance and 2-5% extensor imbalance after 3-4 months.The isokinetic testing provides a tool for the objective evaluation of the knee function. It helps to reveal the high effectiveness of isokinetic rehabilitation protocol leading to the recovery of the patients within 3-4 months after the surgery. It can be assumed based on the results of the present study that harvesting of the semitendinosus/gracilis tendon does not exert any negative influence on the knee flexion strength given the rehabilitation program includes the isotonic training protocol.


Grabeklis A.R.,Federal Medico Biological Agency | Skalny A.V.,Orenburg State University | Nechiporenko S.P.,Federal Medico Biological Agency | Lakarova E.V.,Orenburg State University
Journal of Trace Elements in Medicine and Biology | Year: 2011

In order to improve the monitoring system, watching influence of toxic metals on human health in industrial plants, indicator properties of different biosubstances were compared. Four types of samples (whole blood, plasma, urine, and hair) from 263 workers of the Khimprom chemical plant (Novocheboksarsk, Russia) were subjected to multielement analysis by ICP-AES/ICP-MS. 19-25 chemical elements, including main toxic metals (Cd, Hg, Pb, etc.) were determined. The results were calculated with regard to workers' individual data on occupational exposure to chemical elements. Hair was found to be the most sensitive to toxic and conditionally toxic trace metals: Pb, Mn, Cr, Be, Ni, while occupational contact with macro elements (Na, P), trace metalloids (Si, B) and some other metals (Zn) was not reflected in hair. Whole blood relatively weakly indicated a moderate occupational level of metals except Pb and Mn, but effectively reflected deficiencies of essential elements: I, Cr, and shifts in K/Na ratio, which are likely to be secondary effects of harmful occupational factors. Blood plasma reflected only contact with Be, P; urine - only with Ni. In both whole blood and plasma the changes for the absolute majority of elements were similar. Thus, hair analysis is useful for monitoring the occupational exposure to toxic and conditionally toxic chemical elements, while a general estimation of occupational harmful influence on mineral metabolism requires simultaneous investigation of two biosubstances: hair and whole blood, or hair and blood plasma, with whole blood being more preferable. Analysis of urine is appropriate for monitoring particular chemical elements, e.g. nickel. © 2010 Elsevier GmbH.


Denisov A.A.,Federal Medico Biological Agency | Sclyarov O.D.,Federal State Institution | Salmakov K.M.,Federal State Institution | Shumilov K.V.,Federal State Institution
OIE Revue Scientifique et Technique | Year: 2013

Russia has extensive experience in controlling brucellosis in large and small animals, based on systematic animal health and disease control measures, combined with the use of specific prophylaxis. Widespread application of the live brucellosis vaccine Brucella abortus strain 82 has led to a dramatic reduction in the prevalence of brucellosis. Among the distinctive properties of this vaccine are weak agglutinogenicity and high anti-epizootic efficacy, which allow differentiation between infected animals and vaccinated animals, combined with protection against brucellosis infection. In this paper, the authors review brucellosis epidemiology, diagnostics, the application of vaccines and management procedures that allow the Russian Federation to reduce the overall prevalence of brucellosis.


Lazarev V.N.,Federal Medico Biological Agency | Govorun V.M.,Federal Medico Biological Agency
Applied Biochemistry and Microbiology | Year: 2010

The review presents the current classification of antimicrobial peptides (AMP), which are the main component of innate immunity. The mechanism of their action and the molecular basis of the formation of resistance towards these peptides are described. Data on the use of AMP for the treatment of various infectious diseases, as well as the state of the art in genetic therapy using AMP, are given. © 2010 Pleiades Publishing, Ltd.


Vyatchanina E.S.,Federal Medico Biological Agency
Journal of Trace Elements in Medicine and Biology | Year: 2011

It is known that alcohol possesses embryotoxic, teratogenic, neurotoxic and other effects. Alteration of the trace element and mineral metabolism can be one of the triggering mechanisms of metabolic changes during an alcoholic intoxication. The aim of the recent study was to compare the profiles of elements in brain structures of matured offspring which were born by female rats exposed and non-exposed to alcohol before conception. A decreasing tendency in the levels of all detected macro and trace elements in four brain structures was observed. The most prominent changes were found in the brain cortex. Experimental data show that short term consumption of alcohol by female rats before pregnancy alters the distribution of macro and trace elements in the offspring's brain structures. © 2010 Elsevier GmbH.


Vakhrusheva T.V.,Federal Medico Biological Agency | Gusev A.A.,Federal Medico Biological Agency | Gusev S.A.,Federal Medico Biological Agency | Vlasova I.I.,Federal Medico Biological Agency
Toxicology Letters | Year: 2013

Reduction of thrombogenicity of carbon nanotubes is an important prerequisite for their biomedical use. We assessed the thrombogenic activity of carboxylated single-walled carbon nanotubes (c-SWCNTs) and covalently PEGylated c-SWNCTs (PEG-SWCNTs) by testing the clotting time of platelet poor plasma and platelet aggregation in whole blood samples, and evaluated the impact of human serum albumin on thrombogenicity of carbon nanotubes. Both types of SWCNTs exhibited considerable thrombogenic activity. SWCNTs accelerated plasma clotting, with a lesser effect seen for PEG-SWCNTs. Treatment of SWCNTs with albumin did not affect the SWCNT-induced shortening of clotting time. In whole blood, no discernible differences in the effect of c-SWCNTs and PEG-SWCNTs on platelets were observed. Upon addition of SWCNTs to blood, dose- and time-dependent formation of agglomerates of nanotubes and platelets was demonstrated. Pretreatment of SWCNTs with albumin reduced the platelet aggregation: the number of single platelets increased, and the size of platelet-SWCNT agglomerates decreased dramatically. Hence, addition of albumin may serve to attenuate the adverse, thrombogenic effect of CNTs. © 2013 Elsevier Ireland Ltd.


PubMed | Federal Medico Biological Agency
Type: Journal Article | Journal: Voprosy kurortologii, fizioterapii, i lechebnoi fizicheskoi kultury | Year: 2016

The present review summarizes the data published in the domestic and foreign literature concerning the history of climatic therapy, the current concepts of the mechanisms of action of the climatic and weather factors on the human body, the modern therapeutic modalities and technologies for health promotion. We consider not only the achievements but also the problems arising from insufficient knowledge of the impacts of current climate and extreme weather conditions on the state of human health and some disputable issues of the new methods and technologies of climatic therapy.the promising areas of further research and developments pertaining to climatic therapy as practiced under conditions of the Black Sea coast resorts.

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