Federal Center for Forensic Medical Expertise of Russian Ministry of Health

Moscow, Russia

Federal Center for Forensic Medical Expertise of Russian Ministry of Health

Moscow, Russia
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Leonov S.,Federal Center for Forensic Medical Expertise of Russian Ministry of Health | Zemskova E.,Federal Center for Forensic Medical Expertise of Russian Ministry of Health | Ivanov P.,Federal Center for Forensic Medical Expertise of Russian Ministry of Health
Forensic Science International: Genetics Supplement Series | Year: 2011

The practice of DNA typing of single cells in forensic biological evidence may have peculiarities. For instance, some laser microdissected (LMD) single cell preparations fail to give any PCR products while there is no such a problem with macropreparations from the same sample. As an answer, this study shows that not all the cells individually retrieved from the forensic sample are suitable for analysis. Hence, when going from the bulk to single cell preparations, a bottleneck occurs, which reduces chances for successful DNA typing. For a useful single cell analyses in forensics, single cell DNA profile recovery dependency on the cell type and sample condition must be considered. © 2011 Elsevier Ireland Ltd.


Leonov S.,Federal Center for Forensic Medical Expertise of Russian Ministry of Health | Zemskova E.,Federal Center for Forensic Medical Expertise of Russian Ministry of Health | Timoshenko T.,Federal Center for Forensic Medical Expertise of Russian Ministry of Health | Ivanov P.,Federal Center for Forensic Medical Expertise of Russian Ministry of Health
Forensic Science International: Genetics Supplement Series | Year: 2013

Using the Abbott PLEX-ID PCR/mass spectrometry-based STR assay covering a total of 13 CODIS loci, we typed 310 blood samples from unrelated Russian individuals all had full STR profiles generated with the conventional ABI Identifier Plus/CE system. The MS platform provides for determination of PCR product base compositions thus enabling to determine when an allele has a SNP within the amplified region. Sequence-based polymorphisms relative to the reference alleles were seen in 10 of the 13 loci tested. Up to 15 variant alleles in particular locus were observed. At the 8 loci at least one representative heterozygous individual was found for which the locus is called homozygous by conventional typing. The results demonstrate potential advantages of the sequence-based polymorphisms assay in resolution of samples when limited number of loci can be STR-typed. © 2013 Elsevier Ireland Ltd.

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