Federal Agency of Scientific Organization

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Russia
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Glinushkin A.P.,Federal Agency of Scientific Organization | Beloshapkina O.O.,Russian State Agricultural University | Solovykh A.A.,Orenburg State Agrarian University | Sudarenkov G.V.,Orenburg State Agrarian University | Molnar J.,Federal Agency of Scientific Organization
Acta Phytopathologica et Entomologica Hungarica | Year: 2016

The paper presents the results of a long-term monitoring of bacterial diseases of wheat in the steppe zone of the Southern Ural. A clear domination of the genus Xanthomonas over the genus Pseudomonas has been revealed (60 and 11% of the total samples collected, respectively). The frequency of a winter wheat infection with these two pathogens exceeded that of the spring wheat. The field phytosanitary assessment resulted in the observation of the earlier unknown manifestation of the Xanthomonas infection called as tip bacteriosis. The frequency of wheat infection with Xanthomonas sp. almost did not depend on the relief type, observation period, and sowing date, though the frequency of infection observed for the northern slope and lowland clearly exceeded those observed for other relief types. The frequency of infection with Pseudomonas sp. clearly depended on the sowing date and local relief type reaching the maximum at the lowlands (19-27%), so this relief type can be considered as a "signal" point for the early diagnostics of this pathogen. © 2016 Akadémiai Kiadó, Budapest.


Tishchenko S.,Federal Agency of Scientific Organization | Gabdulkhakov A.,Federal Agency of Scientific Organization | Trubitsina L.,Federal Agency of Scientific Organization | Lisov A.,Federal Agency of Scientific Organization | And 2 more authors.
Acta Crystallographica Section:F Structural Biology Communications | Year: 2015

Laccase (EC 1.10.3.2) is one of the most common copper-containing oxidases; it is found in many organisms and catalyzes the oxidation of primarily phenolic compounds by oxygen. Two-domain laccases have unusual thermostability, resistance to inhibitors and an alkaline optimum of activity. The causes of these properties in two-domain laccases are poorly understood. A recombinant two-domain laccase (SgfSL) was cloned from the genome of Streptomyces griseoflavus Ac-993, expressed in Escherichia coli and purified to homogeneity. The crystals of SgfSL belonged to the monoclinic space group P21, with unit-cell parameters a = 74.64, b = 94.72, c = 117.40 Å, β = 90.672°, and diffraction data were collected to 2.0 Å resolution using a synchrotron-radiation source. Two functional trimers per asymmetric unit correspond to a Matthews coefficient of 1.99 Å3 Da-1 according to the monomer molecular weight of 35.6 kDa. © 2015 International Union of Crystallography.


PubMed | Federal Agency of Scientific Organization
Type: Journal Article | Journal: Acta crystallographica. Section F, Structural biology communications | Year: 2015

Laccase (EC 1.10.3.2) is one of the most common copper-containing oxidases; it is found in many organisms and catalyzes the oxidation of primarily phenolic compounds by oxygen. Two-domain laccases have unusual thermostability, resistance to inhibitors and an alkaline optimum of activity. The causes of these properties in two-domain laccases are poorly understood. A recombinant two-domain laccase (SgfSL) was cloned from the genome of Streptomyces griseoflavus Ac-993, expressed in Escherichia coli and purified to homogeneity. The crystals of SgfSL belonged to the monoclinic space group P21, with unit-cell parameters a = 74.64, b = 94.72, c = 117.40, = 90.672, and diffraction data were collected to 2.0 resolution using a synchrotron-radiation source. Two functional trimers per asymmetric unit correspond to a Matthews coefficient of 1.99(3)Da(-1) according to the monomer molecular weight of 35.6kDa.

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