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Khizhkin E.A.,Russian Academy of Sciences | Ilukha V.A.,Russian Academy of Sciences | Ilyina T.N.,Russian Academy of Sciences | Unzhakov A.R.,Russian Academy of Sciences | And 2 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2010

Activities of antioxidant enzymes, vitamin E level, lactate dehydrogenase isoenzyme spectrum, and effects of melatonin on these parameters were studied in the myocardium of rats aged 6, 12, 18, and 24 months exposed to different illumination regimens. The greatest number of changes was recorded in rats exposed to permanent illumination and light deprivation. Activity of SOD decreased with age, while catalase activity increased. Melatonin treatment did not modify activities of antioxidant enzymes and negligibly modified the level of tocopherol and isoenzyme spectrum of lactate dehydrogenase in rat heart. © 2010 Springer Science+Business Media, Inc.


Vasileva A.K.,Moscow State University | Plotnikov E.Y.,Moscow State University | Kazachenko A.V.,Federal Agency for High Technological Medical Care | Kirpatovsky V.I.,Federal Agency for High Technological Medical Care | Zorov D.B.,Moscow State University
Bulletin of Experimental Biology and Medicine | Year: 2010

Pharmacological preconditioning with insulin and lithium ions prevented the death of renal cells under conditions of ischemia/reperfusion. Preincubation of cells with insulin or lithium ions decreased production of reactive oxygen species after ischemia/reoxygenation. These agents also prevented the development of mitochondrial dysfunction in renal cells induced by ischemia/reoxygenation. It was hypothesized that the protective effects of these agents are related to inhibition of glycogen synthase kinase-3(. This enzyme is inactivated upon phosphorylation of serine residue in position 9. We found that in vivo administration of lithium ions to animals before renal ischemia prevents the development of kidney failure. © 2010 Springer Science+Business Media, Inc.


Mazurov A.V.,Federal Agency for High Technological Medical Care | Titaeva E.V.,Federal Agency for High Technological Medical Care | Khaspekova S.G.,Federal Agency for High Technological Medical Care | Storojilova A.N.,Federal Agency for High Technological Medical Care | And 3 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2011

Characteristics of a new antithrombin DNA-aptamer RE31 were studied. This aptamer inhibited thrombin formation in human plasma catalyzed by exogenous (lengthening of thrombin time) and endogenous thrombin (lengthening of partial prothrombin time and activated partial thromboplastin time). In addition, the aptamer completely suppressed thrombin-induced aggregation of human platelets. On the other hand, RE31 did not reduce amidolytic activity of thrombin towards the short peptide substrate, in other words, did not modify the state of enzyme active center. By the capacity to inhibit clotting reactions, RE31 was superior to the previously described highly effective 31-component antithrombin aptamer 31TBA (thrombin binding aptamer, TBA). The effect of RE31 was species-specific: it inhibited human thrombin activity more effectively than activities of rat and rabbit thrombins. © 2011 Springer Science+Business Media, Inc.


Zlatnik E.Yu.,Federal Agency for High Technological Medical Care | Peredreyeva L.V.,Federal Agency for High Technological Medical Care
Bulletin of Experimental Biology and Medicine | Year: 2012

Metal (Zn, Cu, Fe) nanoparticles, particularly Zn, induce regression of transplanted sarcoma 37 and increase the percentage of dead and degenerated cells. Antitumor effect of metal nanoparticles was not associated with leukopenia or coarse bone marrow abnormalities, but differential leukocyte and bone marrow counts remained changed. © 2012 Springer Science+Business Media, Inc.


Delektorskaya V.V.,Russian Academy of Medical Sciences | Chemeris G.Y.,Russian Academy of Medical Sciences | Zavalishina L.E.,Federal Agency for High Technological Medical Care | Ryazantseva A.A.,Federal Agency for High Technological Medical Care | And 3 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2010

We performed a parallel evaluation of the status of epidermal growth factor receptors EGFR and HER-2 in tumor samples from 31 patients with squamous cell carcinoma of the esophagus. Hyperexpression of proteins was detected by immunohistochemical methods and gene amplifi cation and other chromosome abnormalities were studied using FISH reaction. Evaluation of EGFR status showed that amplifi cation of EGFR gene was present in 25% cases and chromosome 7 polysomy was detected in 29.2% cases positive by protein expression (2+/3+). Immunohistochemically positive EGFR status was confi rmed by the results of FISH reaction for gene amplification and chromosome 7 polysomy in 54.2% cases (p=0.002). During evaluation of HER-2 status in the tumor, hyperexpression of the protein detected histochemically was not confirmed by FISH reaction for detection of amplifi cation of the corresponding gene in 16.1% cases. In 22.6% patients, chromosome 7 polysomy was detected; it was not accompanied by amplifi cation of HER-2 gene, but was related to immunohistochemically positive status of the tumor. Hyperexpression of EGFR protein signifi cantly correlated with the presence of intravascular invasion (p=0.006) and increased depth of invasion (p=0.044), while amplifi cation of EGFR gene (≥2.2) correlated with low differentiation degree of the tumor (p=0.006). The outcome of the disease was not associated with EGFR status at the gene and protein levels, whereas clinical course of the disease in patients with immunohistochemically negative expression of HER-2 protein was more favorable than in patients with positive expression (p=0.004). The results of this study suggest that hyperexpression/amplifi cation of EGFR and hyperexpression of HER-2 are important clinical markers for evaluation of disease prognosis and development of new regimens of targeted therapy for patients with squamous cells carcinoma of the esophagus. © 2010 Springer Science+Business Media, Inc.


Pogorelova T.N.,Federal Agency for High Technological Medical Care | Orlov V.I.,Federal Agency for High Technological Medical Care | Gunko V.O.,Federal Agency for High Technological Medical Care
Bulletin of Experimental Biology and Medicine | Year: 2011

Analysis of the spectrum of amniotic fluid proteins in physiological and abnormal pregnancy using proteomic analysis allowed detection of a number of difference proteins, that are absent or, alternatively, appear in gestosis. Among absent proteins, there were NADPH-dependent carbonyl reductase, epidermal fatty acid-binding protein, haptoglobin, calgranulins A and B. In contrast to proteomic spectrum of amniotic fluid in physiological pregnancy, 7 new proteins appear during gestosis, 3 of them were identified: C area of immunoglobulin K-chain, breast cancer metastasis suppressor-1, and protein-1 containing AIG2-like domain. Possible effects of revealed differences in proteomic spectrum on development of main disturbances during gestosis are discussed. Difference proteins detected in amniotic fluid may serve as gestosis markers. © 2011 Springer Science+Business Media, Inc.


PubMed | Federal Agency for High Technological Medical Care
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2012

Effects of low-intensity microwave radiation with bioactive frequency were studied in rats with transplanted sarcoma 45. After exposure to microwave radiation in the regimen of activation therapy, the antitumor effect was observed in 50% animals (marked inhibition of tumor growth or its partial regression). Light and electron microscopy revealed destructive changes in the tumor tissue, accumulation of activation of immune system cells, and signs of the increase in tumor cell differentiation.


PubMed | Federal Agency for High Technological Medical Care
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2012

Analysis of the spectrum of amniotic fluid proteins in physiological and abnormal pregnancy using proteomic analysis allowed detection of a number of difference proteins, that are absent or, alternatively, appear in gestosis. Among absent proteins, there were NADPH-dependent carbonyl reductase, epidermal fatty acid-binding protein, haptoglobin, calgranulins A and B. In contrast to proteomic spectrum of amniotic fluid in physiological pregnancy, 7 new proteins appear during gestosis, 3 of them were identified: C area of immunoglobulin K-chain, breast cancer metastasis suppressor-1, and protein-1 containing AIG2-like domain. Possible effects of revealed differences in proteomic spectrum on development of main disturbances during gestosis are discussed. Difference proteins detected in amniotic fluid may serve as gestosis markers.


PubMed | Federal Agency for High Technological Medical Care
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2012

Kinetic characteristics of myosin from dog anterior tibial and gastrocnemius muscles after shin bone fracture were studied. Myosin affinity for ATP increased in muscles of the injured and contralateral limbs.


PubMed | Federal Agency for High Technological Medical Care
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2012

We studied the effect of norepinephrine and selective (1)-adrenoceptor blockers on the growth of retinal tissue explants from 10-12-day-old chicken embryos in organotypic culture. The test drugs produced a dose-dependent effect on cell proliferation. Norepinephrine (10(-12) M) and atenolol (10(-4) M) are shown to significantly stimulate explant growth. Combined treatment with norepinephrine (10(-12) M) and atenolol (10(-4) M) results in cumulation of their stimulatory effects. The results suggest that the retina-stimulating effect of norepinephrine is based on its interactions with other types of adrenoceptors.

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