Fasmac Co.

Atsugi, Japan

Fasmac Co.

Atsugi, Japan
SEARCH FILTERS
Time filter
Source Type

Mano J.,Japan National Agriculture and Food Research Organization | Hatano S.,Fasmac Co. | Futo S.,Fasmac Co. | Futo S.,Chiyoda Corporation | And 5 more authors.
Analytical Chemistry | Year: 2014

We developed a reference material of a single DNA molecule with a specific nucleotide sequence. The double-strand linear DNA which has PCR target sequences at the both ends was prepared as a reference DNA molecule, and we named the PCR targets on each side as confirmation sequence and standard sequence. The highly diluted solution of the reference molecule was dispensed into 96 wells of a plastic PCR plate to make the average number of molecules in a well below one. Subsequently, the presence or absence of the reference molecule in each well was checked by real-time PCR targeting for the confirmation sequence. After an enzymatic treatment of the reaction mixture in the positive wells for the digestion of PCR products, the resultant solution was used as the reference material of a single DNA molecule with the standard sequence. PCR analyses revealed that the prepared samples included only one reference molecule with high probability. The single-molecule reference material developed in this study will be useful for the absolute evaluation of a detection limit of PCR-based testing methods, the quality control of PCR analyses, performance evaluations of PCR reagents and instruments, and the preparation of an accurate calibration curve for real-time PCR quantitation. © 2014 American Chemical Society.


Oguchi T.,Japan National Agriculture and Food Research Organization | Oguchi T.,University of Tsukuba | Onishi M.,Fasmac Co. | Mano J.,Japan National Agriculture and Food Research Organization | And 5 more authors.
Journal of the Food Hygienic Society of Japan | Year: 2010

A novel multiplex PCR method was developed for simultaneous event-specific detection of four events of GM maize, i.e., DAS-59122-7, MIR604, MON88017, and MON863. The single laboratory examination of analytical performance using simulated DNA mixtures containing GM DNA at various concentrations in non-GM DNA suggested that the limits of detection (LOD) of the multiplex PCR method were 0.16% for MON863. MIR604, and MON88017, and 0.078% for DAS-59122-7. We previously developed a nonaplex (9plex) PCR method for eight events of GM maize, ie., Bt11, Bt176, GA21, MON810, MON863, NK603, T25, and TC1507. Together with the nonaplex PCR method, the newly developed method enabled the detection and identification of eleven GM maize events that are frequently included in commercial GM seed used in Japan. In addition, this combinational analysis may be useful for the identification of combined event products of GM maize.


PubMed | University of Miyazaki, FASMAC CO., Japan National Agriculture and Food Research Organization and Japan National Institute of Health Sciences
Type: | Journal: Data in brief | Year: 2016

This article is referred to research article entitled Whole genome sequence analysis of unidentified genetically modified papaya for development of a specific detection method (Nakamura et al., 2016) [1]. Real-time polymerase chain reaction (PCR) detection method for unauthorized genetically modified (GM) papaya (Carica papaya L.) line PRSV-YK (PRSV-YK detection method) was developed using whole genome sequence data (DDBJ Sequenced Read Archive under accession No. PRJDB3976). Interlaboratory validation datasets for PRSV-YK detection method were provided. Data indicating homogeneity of samples prepared for interlaboratory validation were included. Specificity and sensitivity test data for PRSV-YK detection method were also provided.


PubMed | University of Miyazaki, FASMAC CO., Japan National Agriculture and Food Research Organization and Japan National Institute of Health Sciences
Type: | Journal: Food chemistry | Year: 2016

Identification of transgenic sequences in an unknown genetically modified (GM) papaya (Carica papaya L.) by whole genome sequence analysis was demonstrated. Whole genome sequence data were generated for a GM-positive fresh papaya fruit commodity detected in monitoring using real-time polymerase chain reaction (PCR). The sequences obtained were mapped against an open database for papaya genome sequence. Transgenic construct- and event-specific sequences were identified as a GM papaya developed to resist infection from a Papaya ringspot virus. Based on the transgenic sequences, a specific real-time PCR detection method for GM papaya applicable to various food commodities was developed. Whole genome sequence analysis enabled identifying unknown transgenic construct- and event-specific sequences in GM papaya and development of a reliable method for detecting them in papaya food commodities.


Aida T.,Tokyo Medical and Dental University | Chiyo K.,Tokyo Medical and Dental University | Usami T.,Laboratory of Recombinant Animals | Ishikubo H.,Tokyo Medical and Dental University | And 8 more authors.
Genome Biology | Year: 2015

Although the CRISPR/Cas system has enabled one-step generation of knockout mice, low success rates of cassette knock-in limit its application range. Here we show that cloning-free, direct nuclear delivery of Cas9 protein complex with chemically synthesized dual RNAs enables highly efficient target digestion, leading to generation of knock-in mice carrying a functional cassette with up to 50% efficiency, compared with just 10% by a commonly used method consisting of Cas9 mRNA and single guide RNA. Our cloning-free CRISPR/Cas system facilitates rapid one-step generation of cassette knock-in mice, accelerating functional genomic research by providing various in vivo genetic tools. © 2015 Aida et al.; licensee BioMed Central.


PubMed | Hiroshima University, FASMAC Co., Tokyo Medical and Dental University, Laboratory of Recombinant Animals and Keio University
Type: | Journal: Genome biology | Year: 2015

Although the CRISPR/Cas system has enabled one-step generation of knockout mice, low success rates of cassette knock-in limit its application range. Here we show that cloning-free, direct nuclear delivery of Cas9 protein complex with chemically synthesized dual RNAs enables highly efficient target digestion, leading to generation of knock-in mice carrying a functional cassette with up to 50% efficiency, compared with just 10% by a commonly used method consisting of Cas9 mRNA and single guide RNA. Our cloning-free CRISPR/Cas system facilitates rapid one-step generation of cassette knock-in mice, accelerating functional genomic research by providing various in vivo genetic tools.


Monden Y.,Okayama University | Takasaki K.,FASMAC Co. | Futo S.,FASMAC Co. | Niwa K.,Tohoku University | And 3 more authors.
Journal of Biotechnology | Year: 2014

In many crops species, the development of a rapid and precise cultivar discrimination system has been required for plant breeding and patent protection of plant cultivars and agricultural products. Here, we successfully evaluated strawberry cultivars via a novel method, namely, the single tag hybridization (STH) chromatographic printed array strip (PAS) using the PCR products of eight genomic regions. In a previous study, we showed that genotyping of eight genomic regions derived from FaRE1 retrotransposon insertion site enabled to discriminate 32 strawberry cultivars precisely, however, this method required agarose/acrylamide gel electrophoresis, thus has the difficulty for practical application. In contrast, novel DNA detection method in this study has some great advantages over standard DNA detection methods, including agarose/acrylamide gel electrophoresis, because it produces signals for DNA detection with dramatically higher sensitivity in a shorter time without any preparation or staining of a gel. Moreover, this method enables the visualization of multiplex signals simultaneously in a single reaction using several independent amplification products. We expect that this novel method will become a rapid and convenient cultivar screening assay for practical purposes, and will be widely applied to various situations, including laboratory research, and on-site inspection of plant cultivars and agricultural products. © 2014 The Authors.


PubMed | Tohoku University, FASMAC Co. and Okayama University
Type: | Journal: Journal of biotechnology | Year: 2014

In many crops species, the development of a rapid and precise cultivar discrimination system has been required for plant breeding and patent protection of plant cultivars and agricultural products. Here, we successfully evaluated strawberry cultivars via a novel method, namely, the single tag hybridization (STH) chromatographic printed array strip (PAS) using the PCR products of eight genomic regions. In a previous study, we showed that genotyping of eight genomic regions derived from FaRE1 retrotransposon insertion site enabled to discriminate 32 strawberry cultivars precisely, however, this method required agarose/acrylamide gel electrophoresis, thus has the difficulty for practical application. In contrast, novel DNA detection method in this study has some great advantages over standard DNA detection methods, including agarose/acrylamide gel electrophoresis, because it produces signals for DNA detection with dramatically higher sensitivity in a shorter time without any preparation or staining of a gel. Moreover, this method enables the visualization of multiplex signals simultaneously in a single reaction using several independent amplification products. We expect that this novel method will become a rapid and convenient cultivar screening assay for practical purposes, and will be widely applied to various situations, including laboratory research, and on-site inspection of plant cultivars and agricultural products.


Masamura N.,House Foods Group Inc. | Kikuchi R.,FASMAC CO. | Nagatomi Y.,FASMAC CO.
Bunseki Kagaku | Year: 2014

We developed a method to identify plant materials by amplifying by PCR the internal transcribed spacer 1 (ITS 1) region located between the 18S ribosomal RNA (18S rRNA) and the 5.8S ribosomal RNA (5.8S rRNA) genes using plant universal primers designed on the two genes, and determining the nucleotide sequence of the amplified product. The primer designed on the 18S rRNA gene is universal to a wide range of plant species, designed from a nucleotide sequence not found in fungi or yeast, and thus is applicable to plant materials contaminated with fungi/yeast. The current method correctly identified 36 of 45 commercially available plant species, including 9 plants identified up to the genus level. Onion scale leaves heated at 105 °C for 16 hrs and onion scale leaves contained in retort sauce were also identified. The method was then tested for its ability to identify possible contaminants in food, including peanut seed coat, calyxes of apples and pumpkins, bamboo, wood pieces and the pericarp of frozen eggplants. PCR products were obtained from the peanut, pumpkin and apple materials, and these plants were identified up to the species or genus level. These results suggest the potential of the current method for identifying plant contaminants in food. © 2014 The Japan Society for Analytical Chemistry.


PubMed | Japan National Institute of Advanced Industrial Science and Technology, FASMAC Co. and Aichi University
Type: Journal Article | Journal: PloS one | Year: 2014

From the 16th to the 18th centuries in Japan, saltpeter was produced using a biological niter-bed process and was formed under the floor of gassho-style houses in the historic villages of Shirakawa-go and Gokayama, which are classified as United Nations Educational, Scientific and Cultural Organization (UNESCO) World Heritage Sites. The relict niter-beds are now conserved in the underfloor space of gassho-style houses, where they are isolated from destabilizing environmental factors and retain the ability to produce nitrate. However, little is known about the nitrifying microbes in such relict niter-bed ecosystems. In this study, the microbial community structures within nine relict niter-bed soils were investigated using 454 pyrotag analysis targeting the 16S rRNA gene and the bacterial and archaeal ammonia monooxygenase gene (amoA). The 16S rRNA gene pyrotag analysis showed that members of the phyla Proteobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Gemmatimonadetes, and Planctomycetes were major microbial constituents, and principal coordinate analysis showed that the NO3-, Cl-, K+, and Na+ contents were potential determinants of the structures of entire microbial communities in relict niter-bed soils. The bacterial and archaeal amoA libraries indicated that members of the Nitrosospira-type ammonia-oxidizing bacteria (AOB) and Ca. Nitrososphaera-type ammonia-oxidizing archaea (AOA), respectively, predominated in relict niter-bed soils. In addition, soil pH and organic carbon content were important factors for the ecological niche of AOB and AOA in relict niter-bed soil ecosystems.

Loading Fasmac Co. collaborators
Loading Fasmac Co. collaborators