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Karami-Osboo R.,Shiraz University of Medical Sciences | Miri R.,Shiraz University of Medical Sciences | Javidnia K.,Shiraz University of Medical Sciences | Kobarfard F.,University of Tehran | And 2 more authors.
Journal of Food Science and Technology | Year: 2015

A method based on dispersive liquid-liquid microextraction (DLLME) was developed for the quantitative extraction of Ochratoxin A (OTA) from raisin samples. The influence of various parameters on the recovery of OTA such as type and volume of DLLME extractant, centrifuging and sonication time, also volume of deionized water was investigated. Recovery values under the optimum conditions were between 68.6 and 85.2 %, the inner and intra-day precision expressed as relative standard deviation (RSD%, n = 3), were less than 15 % at spiking levels of 2.5–30 μg kg−1. Linearity was studied from 0.5 to 30 μg L−1, and the limits of detection (LOD) and quantification (LOQ) were 0.7 and 2.0 μg kg−1, respectively. Real samples were analyzed by DLLME method and compared with confirmative immunoaffinity Column Chromatography (IAC) clean-up. Low cost, simplicity of operation, speed and minimum consumption of organic solvent were the main advantages of proposed method. The mean contamination of samples was 0.88 μg kg−1 that was lower than European Legal Limit. © 2013, Association of Food Scientists & Technologists (India).

Karami-Osboo R.,Shiraz University of Medical Sciences | Miri R.,Shiraz University of Medical Sciences | Javidnia K.,Shiraz University of Medical Sciences | Shojaee M.H.,Faroogh Life Science Research Laboratory No 962 | Kobarfard F.,Shahid Beheshti University
Analytical Methods | Year: 2015

Sulfonamides are heat-stable antibacterial drugs and their residues in milk increase the risk of human exposure and may cause food-borne illness. In this study, Fe3O4 magnetic nanoparticles (Fe3O4 MNPs) have been successfully used for the extraction of sulfadiazine (SDZ) and sulfathiazole (STZ) from milk samples. The effect of the amount of magnetic adsorbents, volume of extracted milk and sample pH were investigated and optimized. The experimental results showed that the suggested method possessed suitable analytical performance. Linearity was obtained over a concentration range of 0.25, 0.5, 1.0, 2.0 and 4 MRL with regression coefficients ranging from 0.9985 to 0.9978 for SDZ and STZ. The limits of detection (LOD) and quantification (LOQ), for the two analytes, were 10 and 30 ng mL-1, respectively. The proposed method was used for the analysis of different milk samples, and excellent recoveries in the range of 92.9-102.4% were obtained. These results indicated that the proposed method can be broadly used in the monitoring of low concentrations of drugs in milk samples and open fascinating perspectives in future studies. © 2015 The Royal Society of Chemistry.

Tahmouzi S.,Ramin Agricultural and Natural Resources | Tahmouzi S.,University of Tehran | Ghasemlou M.,University of Tehran | Aliabadi F.S.,Faroogh Life Science Research Laboratory No 962 | And 3 more authors.
Journal of Food Processing and Preservation | Year: 2013

Temperature abuse of skipjack tuna (Katsuwonus pelamis) was carried out in order to assess the histamine formation and bacterial growth in fish-processing facilities where fish can be exposed to high temperatures for short periods of time. Histamine production and bacteriological quality were monitored under different defrosting time and abuse conditions (4, 10, 15, 25 and 30C). A high-performance liquid chromatography method involving an isocratic elution was validated for the determination of histamine in the samples. Most of the fish samples showed higher contents than the allowable level, whereas samples stored at 4C had negligible histamine levels. The highest level of histamine formed was 185.21mg/kg after 96h of defrosting at 10C. In the samples stored at 30C, the histamine contents markedly increased up to 166.91mg/kg after 96h, while the histamine content began to gradually increase following defrosting at 10C. Aerobic plate counts reached 7.2×105cfu/g after 24h at 25C. The dominant microbial group was the Enterobacteriaceae family throughout the storage period. The optimal temperature for bacterial growth was 30C, whereas the optimal temperature for histamine production was 10C. © 2012 Wiley Periodicals, Inc.

Karami-Osboo R.,Shiraz University of Medical Sciences | Maham M.,Islamic Azad University | Miri R.,Shiraz University of Medical Sciences | AliAbadi M.H.S.,Faroogh Life Science Research Laboratory No 962 | And 2 more authors.
Food Analytical Methods | Year: 2013

A fast and simple method for the extraction of deoxynivalenol (DON) from wheat flour using dispersive liquid-liquid microextraction (DLLME) followed by high-performance liquid chromatography-UV detection has been developed and compared with immunoaffinity column cleanup (IAC) process. The influence of several important parameters on the extraction efficacy was studied. Under optimized conditions, a linear calibration curve was obtained in the range of 50-1,000 μg/L. Average recoveries of DON from spiked wheat samples at levels of 500 μg/kg for DLLME and IAC ranged from 72. 9 ± 1. 6 and 85. 5 ± 3. 1, respectively. A good correlation was found for spiked samples between DLLME and IAC methods. The limit of detection was 125 and 50 μg/kg for DLLME and IAC method, respectively. Advantages of DLLME method with respect to the IAC have been pointed out. © 2012 Springer Science+Business Media, LLC.

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