Santa Maria do Suaçuí, Brazil
Santa Maria do Suaçuí, Brazil

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Leite L.A.C.,Federal University of São Paulo | Leite L.A.C.,Federal University of Pernambuco | Almeida M.S.,Hematologia | Kimura E.S.,Farmaceutica Bioquimica | And 4 more authors.
Jornal Brasileiro de Patologia e Medicina Laboratorial | Year: 2010

Introduction and objective; Multiple myeloma is an incurable malignancy characterized by the proliferation of a single clone of plasma cells in bone marrow. The aim of this study was to evaluate the frequency and prognostic value of the expression of aberrant phenotypes in patients with multiple myeloma by multiparametric flow cytometry. Methods; The study was carried out at Department of Hematology and Hemotherapy of Federal University of São Paulo and 30 patients with MM were analyzed prospectively. In an attempt to identify myeloma cells by flow cytometry (FACSCalibur, BD), specific monoclonal antibodies anti-CD138, anti-CD38 and anti-CD45 were used for the selection of plasma cells. The control group comprised four healthy bone marrow donors. Results: All myeloma plasma cells expressed at least one aberrant phenotype and CD56 +++, CD117++, CD33++, CD13++ and CD28++ markers were more frequently observed in 88% of patients. Lymphoid markers were found in cases with a higher number of aberrant phenotypes. Discussion: CD56+++and CD28++ antigens showed high levels of β2-microglobulin, which are associated with more aggressive stages of the disease and larger tumor mass. The absence of adhesion molecule CD56 was associated with high levels of β2M and calcium ion, showing that this finding may have prognostic value. Conclusion; From this study it was concluded that the aberrant phenotypes are present in most cases of MM, and immunophenotyping by multiparametric flow cytometry is a useful tool to distinguish normal plasma cells from myeloma plasma cells.

Parmezan S.N.,Farmaceutica Bioquimica | Pasternak J.,Medicina | Dezene A.H.P.,Federal University of São Paulo | Dalla Valle Martino M.,Clinico do Hospital Israelita Albert Einstein | De Souza A.V.,University of Sao Paulo
Jornal Brasileiro de Patologia e Medicina Laboratorial | Year: 2011

Introduction: Human metapneumovirus (HMPV) is responsible for respiratory infections in children and immunocompromised adults and elders. It is commonly diagnosed by immunofluorescence or molecular biology. Objective: To detect HMPV in clinical samples by polymerase chain reaction (PCR) and direct imunofluorescence (DIF) methods. Results: Two percent of 202 samples were positive for DIF and 4% of them for reverse transcriptase PCR (RT-PCR), respectively. Considering RT-PCR as gold standard, DIF sensitivity and specificity were 50% and 100%, respectively. Conclusion: Not only does the study show that RT-PCR is the best method for HMPV detection in clinical respiratory samples but it also substantiates the importance of test standardization in laboratory routine.

The effect of the addition of inulin-type fructans of different degrees of polymerization (DP) on the physicochemical and microbiological characteristics and storage stability (4°C for 28 days) of nonfat probiotic yoghurt was investigated. The yoghurts were prepared using traditional lactic culture (Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus) and Lactobacillus paracasei ssp. paracasei as probiotic. The formulations contained inulin- type fructans of DP: low (P95 oligofructose, DP 5), medium (ST inulin, DP 10) or high (HP inulin, DP 23) in a concentration of 2g 100g of milk-1, meeting the current Brazilian legislation and, thus their claim of functional properties could be utilized; and were compared to a Normal formulation (without inulin-type fructans addition). The addition of inulin-type fructans with different DP did not alter the L*, a* and b* color parameters, the syneresis and the viability of the probiotic culture in yoghurts. Yoghurts with inulin-type fructans of low DP were more acidic (lower pH values and higher values of acidity). The high DP inulin caused a reduction in the firmness of yoghurt. The products contained Lactobacillus paracasei ssp. paracasei at concentrations higher than that recommended to consider them as probiotic foods throughout the storage period. The refrigerated storage resulted in decreased pH and viability of the probiotic culture and increased acidity and syneresis of yoghurt, regardless of the use of inulin-type fructans. It can be concluded that the addition of inulin-type fructans of different DP does not adversely affect the physicochemical and microbiological characteristics of yoghurts and their stability to storage, thus, the selection of the type to be used depend on the purpose of the manufacturer and the intended use.

Anziliero D.,Federal University of Santa Maria | Bassi E.,Farmaceutica Bioquimica | Pain K.M.,Medica Veterinaria autonoma | Valle S.D.F.,Federal University of Rio Grande do Sul | Kreutz L.C.,University Of Passo Fundo
Ciencia Animal Brasileira | Year: 2013

The natural immune response to infectious agents is partially mediated by a group of proteins named acute phase proteins; one of the major and most important proteins from the acute phase is the C-Reactive Protein (CRP). The aim of thepresent study was to investigate the correlation between alterations on white blood cell counts (WBC) and the presence of circulating CRP. Blood samples from 70 dogs were submitted to hematological examination and detection of CRP; in addition, blood samples from 12 dogs experimentally inoculated with an inactivated sample of Micrococcus luteus (ATCC 7468) were also analyzed. According to the hematological parameters and CRP values, the samples were classified in 5 different groups: group A) dogs without CRP and normal WBC; group B) dogs with elevated levels of CRP but normal WBC; group C) dogs with elevated CRP values and altered WBC (neutrophilia and leukocytosis); group D) dogs with both CRP and increased neutrophils values; and group E) dogs with elevated CRP values but with leukopenia. Dogs experimentally inoculated with M.luteus had a significant and promt increase in CRP values between 24 and 48 hr p.i., which declined up to the 10th day when the experiment was completed. In these dogs, a discrete neutrophilia and leukocytosis was observed coincidently withthe CRP elevation. Thus, it can be suggested that the detection of CRP in the blood of dogs should be used as an indication of an infection or inflammation and, because of the low amount of blood needed, low cost and easiness to perform thetest, monitoring CRP levels might be useful to evaluate the recovery from an infection disease.

De Araujo Borba C.M.,Microbiologia | De Oliveira V.M.,Farmaceutica Bioquimica | Arend L.N.V.S.,Microbiologia | Pilonetto M.,Farmaceutica Bioquimica | Pilonetto M.,Pontifical Catholic University of Parana
Jornal Brasileiro de Patologia e Medicina Laboratorial | Year: 2012

Introduction: The production of Klebsiella pneumoniae carbapenemases enzymes (KPC) has become an important and worrisome resistance mechanism. Furthermore, tests that combine high sensitivity and high specificity for the detection of these enzymes are scarce. Objective: To validate the inhibition test by 3-aminophenyl boronic acid as a phenotypic screening method for KPC-producing strains by comparing the results with confirmatory polymerase chain reaction testing (PCR). Methods: We evaluated the use of 3-aminophenyl boronic acid applied on disks with imipenem, meropenem and ertapenem antibiotics. 36 strains were positive and 12 were negative, all confirmed by PCR. Three different concentrations of boronic acid were also tested: 300, 400 and 600 μg. Results: Among the positive strains, the results were more accurate with the addition of the compound to the ertapenem disk, presenting 100 % specificity and 50% sensitivity. Conclusion: Further studies are required, mainly regarding the standardization of the technique and materials, since the method seems to be promising as to the screening of KPC strains.

Rigatti F.,Federal University of Santa Maria | Tizotti M.K.,Federal University of Santa Maria | Horner R.,Federal University of Santa Maria | Domingues V.O.,Farmaceutica Bioquimica | And 5 more authors.
Revista da Sociedade Brasileira de Medicina Tropical | Year: 2010

Introduction: This study aimed to characterize the prevalence and susceptibility profile to oxacillin-resistant Coagulase-negative Staphylococci strains isolated from blood cultures in a teaching hospital, located in Santa Maria, RS. In addition, different methodologies for phenotypic characterization of mecA-mediated oxacillin resistance were compared with genotypic reference testing. Methods: After identification (MicroScan® - Siemens), the isolates were tested for antimicrobial sensitivity using disk diffusion and automation (MicroScan® - Siemens). The presence of mecA gene was identified by the polymerase chain reaction molecular technique. Results: The most common species was Staphylococcus epidermidis (n=40, 67%). The mecA gene was detected in 54 (90%) strains, while analysis of the sensitivity profiles revealed a high rate of resistance to multiple classes of antimicrobial drugs. However, all isolates were uniformly sensitive to vancomycin and tigecycline. The cefoxitin disk was the phenotypic method that best correlated with the gold standard. Conclusions: Analysis of the clinical significance of CoNS isolated from hemocultures and the precise detection of oxacillin resistance represent decisive factors for the correct choice of antibiotic therapy. Although vancomycin constitutes the normal treatment in most Brazilian hospitals, reduction in its use is recommended.

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