Khabarovsk Vtoroy, Russia

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Keita A.K.,French National Center for Scientific Research | Bassene H.,IRD Montpellier | Tall A.,Institute Pasteur Of Dakar | Sokhna C.,IRD Montpellier | And 7 more authors.
PLoS Neglected Tropical Diseases | Year: 2011

Background: Tropheryma whipplei is known as the cause of Whipple's disease, but it is also an emerging pathogen, detected in stool, that causes various chronic localized infections without histological digestive involvement and is associated with acute infections, including gastroenteritis and bacteremia. Methods/Principal Findings: We conducted a study in 2008 and 2009 using 497 non-diarrheic and diarrheic stool samples, 370 saliva samples, 454 sera samples and 105 samples obtained from water samples in two rural Sine-Saloum villages (Dielmo and Ndiop) in Senegal. The presence of T. whipplei was investigated by using specific quantitative PCR. Genotyping was performed on positive samples. A serological analysis by western blotting was performed to determine the seroprevalence and to detect seroconversion. Overall, T. whipplei was identified in 31.2% of the stool samples (139/446) and 3.5% of the saliva samples (13/370) obtained from healthy subjects. The carriage in the stool specimens was significantly (p&10 -3) higher in children who were between 0 and 4 years old (60/80, 75%) compared to samples obtained from individuals who were between 5 to 10 years old (36/119, 30.2%) or between 11 and 99 years old (43/247, 17.4%). The carriage in the stool was also significantly more common (p = 0.015) in subjects with diarrhea (25/51, 49%). We identified 22 genotypes, 16 of which were new. Only one genotype (#53) was common to both villages. Among the specific genotypes, one (#52) was epidemic in Dielmo (15/28, 53.4%, p&10 -3) and another (#49) in Ndiop (27.6%, p = 0.002). The overall seroprevalence was estimated at 72.8% (291/400). Seroconversion was detected in 66.7% (18/27) of children for whom PCR became positive in stools between 2008 and 2009. Conclusions/Significance: T. whipplei is a common bacterium in the Sine-Saloum area of rural Senegal that is contracted early in childhood. Epidemic genotypes suggest a human transmission of the bacterium. © 2011 Keita et al.


Ukrainets I.V.,National University of Pharmacy | Petrushova L.A.,National University of Pharmacy | Shishkina S.V.,Ukrainian Academy of Sciences | Sim G.,Far Eastern State Medical University
Chemistry of Heterocyclic Compounds | Year: 2015

The alkylation of methyl 4-hydroxy-1-methyl-2,2-dioxo-1H-2λ6,1-benzothiazine-3-carboxylate in DMSO or acetone with ethyl iodide at 25°C gave a mixture of 3-ethyl- and 4-ethoxy-substituted derivatives, in complete agreement with quantum-chemical calculations. The ratio of C- and O-alkylation products changed depending on the alkali metal carbonate used as a base. The reaction practically did not proceed in anhydrous THF or water due to the low nucleophilicity of the anion. © 2015 Springer Science+Business Media New York.


PubMed | Ukrainian Academy of Sciences, National University of Pharmacy and Far Eastern State Medical University
Type: Journal Article | Journal: Scientia pharmaceutica | Year: 2017

As potential new analgesics, the corresponding 4-hydroxy-2,2-dioxo-1


Mediannikov O.,Aix - Marseille University | Socolovschi C.,Aix - Marseille University | Bassene H.,Aix - Marseille University | Diatta G.,Aix - Marseille University | And 5 more authors.
Emerging Infectious Diseases | Year: 2014

As malaria cases in Africa decline, other causes of acute febrile illness are being explored. To determine incidence of Borrelia crocidurae infection during June 2010- October 2011, we collected 1,566 blood specimens from febrile patients in Senegal. Incidence was high (7.3%). New treatment strategies, possibly doxycycline, might be indicated for febrile patients.


Ryzhavskii B.Ya.,Far Eastern State Medical University | Litvintseva Ve.M.,Far Eastern State Medical University
Bulletin of Experimental Biology and Medicine | Year: 2012

Here we present the results of histochemical detection of 3β-hydroxysteroid dehydrogenase in neurons of the neocortex, cerebellum, and brainstem. The positive reaction was observed in a low number of neocortical, midbrain, and pontine neurons. The maximum number of positive neurons was detected in the cerebellar cortex (Purkinje cells) and hippocampus. © 2012 Springer Science+Business Media, Inc.


Ryzhavskii B.Y.,Far Eastern State Medical University | Zadvornaya O.V.,Far Eastern State Medical University
Bulletin of Experimental Biology and Medicine | Year: 2012

3β-Hydroxysteroid dehydrogenase is a key enzyme in the synthesis of steroid hormones in steroid-producing organs, including the brain producing neurosteroids. 3β-Hydroxysteroid dehydrogenase activity can be a marker of steroid-producing cells. We present the results of histochemical assay of this enzyme in the neocortex, hippocampus, and cerebellar cortex of go nadectomized prepubertal rats. The positive reaction was detected in hippocampal neurons, ganglionic layer cells of the cerebellar cortex (Purkinje cells), and solitary neocortical neurons of male and female rats. Gonadectomy significantly increased enzyme activity in neocortical (layer V) and hippocampal neurons and had no effect on the intensity of the reaction in Purkinje cells. © 2012 Springer Science+Business Media New York.


Ryzhavskii B.Y.,Far Eastern State Medical University
Bulletin of experimental biology and medicine | Year: 2012

Morphometric characteristics and free radical oxidation in neurons of the neocortex and hippocampus of male and female rats were studied 1 month after administration of Sustanon-250 in a dose of 8 mg/kg during the pubertal period of ontogeny. The weight of the brain was shown to decrease in males. In both female and male rats, the width of the anterior parietal lobe and the numerical density of neocortical and hippocampal neurons decreased. Sex differences were found in free radical oxidation in the cerebral cortex. The intensity of this process increased significantly in females, but decreased in males.


Sirotin B.Z.,Far Eastern State Medical University | Korneeva N.V.,Far Eastern State Medical University
Terapevticheskiĭ arkhiv | Year: 2016

AIM: To study the influence of smoking cessation on the microcirculatory bed (MCB) in 33 healthy young people.SUBJECTS AND METHODS: The investigation enrolled 33 healthy young people (male/female (M/F) ratio, 20:13; mean age, 21.6±0.5 years) who had quit smoking 1 month to 10 years (mean age 2.5±0.5 years) before. Comparable groups consisted of 15 smokers (M/F ratio, 5:10; mean age, 21.5±0.8 years) and 18 nonsmokers (M/F ratio, 6:12; mean age, 21±0.3 years). Computer-assisted video-biomicroscopy of the bulbar conjunctiva was carried out. When assessing theRESULTS: attention was focused on microvessel diameter changes in the patients who had quit smoking compared to the smokers and nonsmokers.RESULTS: As compared to the smokers, those who had quit smoking showed a significant increase in the mean diameters of arterioles (13.01±0.41 and 18.33±0.65 µm; р<0.001) and capillaries (9.2±0.23 and 9.89±0.19 µm; р<0.05). The number of functioning capillaries per mm2 of the conjunctival surface did not differ from that in the nonsmokers (8.01±0.21 and 8.25±0.44; р>0.05). Thus, the rarefication phenomenon reflecting the influence of smoking on the MCB vessels at a young age undergoes rapidly regression. There are increases in the diameter of arterioles and the number of functioning capillaries just in the first months of smoking cessation (р<0.01).CONCLUSION: The considerably increased diameters of arterioles and capillaries and the larger number of functioning capillaries per mm2 of the conjunctival surface suggest that smoking cessation at a young age have a positive impact on MCB vessels.Abstract available from the publisher.


Ryzhavskii B.Y.,Far Eastern State Medical University | Lebed'ko O.A.,Far Eastern State Medical University
Bulletin of Experimental Biology and Medicine | Year: 2016

We studied the effect of bleomycin (1 mg/kg) in 1-day-old rat pups (single intraperitoneal injection) and 4-day-old pups (3 injections on postnatal days 1, 2, and 3). The formation of hyaline membranes against the background of oxidative stress was documented in all bleomycin-treated rats. Repeated injection of bleomycin (4-day-old pups) led to more pronounced destructive alterations in the lungs characterized by the formation of microcysts and atelectases as well as hypertrophy of the interstitial connective tissue. These alterations persisted in 14-day-old rats. Administration of bleomycin during the neonatal period can be a promising approach to simulation of hyaline membrane disease (respiratory distress syndrome). © 2016 Springer Science+Business Media New York


Lebed'ko O.A.,Far Eastern State Medical University | Ryzhavskii B.Y.,Far Eastern State Medical University | Demidova O.V.,Far Eastern State Medical University
Bulletin of Experimental Biology and Medicine | Year: 2015

We studied the protective effect of antioxidant echinochrome A on bleomycin-induced pulmonary fi brosis in rats at the early stages of postnatal ontogeny. Administration of echinochrome A was shown to reduce the severity of bleomycin-induced oxidative stress in the lungs, prevented the development of hypertrophy of interalveolar connective tissue and peribronchial lymphoid infi ltration, and normalized the ratio of volume densities of interalveolar septa and alveolar lumen. © 2015, Springer Science+Business Media New York.

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