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Li J.,Guangzhou Psychiatric Hospital | Chang S.-S.,University of Hong Kong | Chang S.-S.,Ju Shan Hospital | Yip P.S.F.,University of Hong Kong | And 7 more authors.
BMC Public Health | Year: 2014

Background: There has been a dramatic increase in internal migrant workers in China over recent decades, and there is a recent concern of poor mental health particularly amongst younger or "new generation" migrants who were born in 1980 or later. Methods: A cross-sectional study was conducted in Guangzhou city between May and July in 2012. Mental wellbeing was measured using the World Health Organization Five-item Well-Being Index Scale and the 36 Item Short Form Health Survey mental health scale. Linear and logistic regression models were used to investigate the differences between migrant workers and their urban counterparts and between younger and older migrants. Results: Migrant workers (n = 914) showed a small but significant advantage in mental wellbeing compared to their urban counterparts (n = 814). There was some evidence for age modification effect (p for interaction = 0.055-0.095); better mental wellbeing in migrants than urbanites were mainly seen in the older compared to the younger group, and the difference attenuated somewhat after controlling for income satisfaction. Older migrants showed better mental health than younger migrants. Factors that were independently associated with poor mental health in migrants included being male, longer working hours, and income dissatisfaction, whilst older age, factory job, high income, and increased use of social support resources were associated with reduced risk. Conclusions: Efforts to promote mental health amongst migrant workers may be usefully targeted on younger migrants and include measures aimed to improve working conditions, strengthen the social support network, and address age-specific needs.


LI S.-c.,Family Planning Research Institute of Guangdong Province | PAN P.,Family Planning Research Institute of Guangdong Province | YAO S.-z.,Sun Yat Sen University | FENG M.,Family Planning Research Institute of Guangdong Province | And 3 more authors.
Journal of Reproduction and Contraception | Year: 2010

Objective: To investigate the relation between hysteroscopic finding and pinopodes expression, meanwhile to explore whether hysteroscopic finding was a predictor of reproductive outcome for infertile women. Methods: A total of 79 infertile patients underwent hysteroscopic assessement of the midsecretory endometrium and hormonal measurement 7-9 d after a spontaneous ovulation assessed with transvaginal ultrasound. Pinopodes expression by scanning electron microscope was examined simultaneously in randomly selected 32 patients. All patients were followed up to determine their reproductive outcomes. Results: Twenty-six (32.9%) patients were diagnosed as having 'good' midsecretory endometrium and 53 (67.1%) as 'poor' midsecretory endometrium by hysteroscopy. There were no significant differences of endometrial thickness and pattern in the 'good' group compared with the 'poor' group. Serum progesterone level in the 'good' group was apparently higher than that in the 'poor' group (P=0.025), but no correlation was observed between serum oestradiol level and hysteroscopic finding. Significant differences of the development stage and number of pinopodes were observed between the two groups (P<0.05). The pregnancy rate was significantly higher in the 'good' group (53.9%) than in the 'poor' group (26.4%) (P=0.017). Conclusion: The hysteroscopic appearance of the midsecretory endometrium is associated with serum progesterone level and pinopodes expression, which provides prognostic information with regard to the reproductive outcome of infertile women. © 2010 The Editorial Board of Journal of Reproduction and Contraception.


Zheng W.-W.,Family Planning Research institute of Guangdong Province | Cup G.-H.,Sun Yat Sen University | Chen X.-G.,Sun Yat Sen University | Wei X.-C.,Family Planning Research institute of Guangdong Province | Zhong X.-M.,Family Planning Research institute of Guangdong Province
Journal of Reproduction and Contraception | Year: 2011

Objective: To investigate whether germ like cells isolated from embryoid body formed by mouse embryonic stem cells could survive and initiate spermatogenesis in seminiferous tubules of adult mice. Methods: SSEA-1+ cells were isolated from embryoid bodies prepared from mouse EGFP-ES cells, after retinoic acid treatment, the cells were detected for the expression of alkaline phosphatase, Rnh2, Stella, fragilis, Texl4, Sry, Hsp90-α, Stra8 and inte grin a6, and then, the cells were transplanted into seminiferous tubules of busulfan-treated adult mice. Results: Six days after retinoic acid treatment, alkaline phosphatase expressing cells could still be found in embryoid body (EB) derived cells, indicating the existence of retinoic acid-resistant primordial germ cells. When the SSEA-1+ cells isolated from embryoid bodies were stimulated with retinoic acid for 6 days, some of these cells expressed cell markers of Hsp90-α, Stra8 and inte grin α6, resembling the expression profile of spermatogonial stem cells. Forty-five days after cell transplantation, a little amount of GFP-expressing cells attached to the basement membrane of seminiferous tubule and formed small colonies; Three months later, these cells started amplification in the form of cell chains with varied length, and moving towards the lumen of the seminiferous tubules. Five months after the transplantation, multilayered cell mass was found in seminiferous tubules of two, out of four recipient mice. There was no GFP-expressing cells existed in non-cell-transplanted seminiferous tubules. Conclusion: In our study, although full-termed spermatogenesis was not observed in all of the recipients, the results did indicate that the embryoid body contains germ like cells, and these cells can survive and initiate amplification in seminiferous tubules of adult mouse. © 2011 The Editorial Board of Journal of Reproduction and Contraception.


Fu Y.,Sun Yat Sen University | Fu Y.,Guangzhou Blood Center | Qin W.,Family Planning Research Institute of Guangdong Province | Qin W.,Sun Yat Sen University | And 14 more authors.
PLoS ONE | Year: 2012

Background: Recently in China, HCV 6a infection has shown a fast increase among patients and blood donors, possibly due to IDU linked transmission. Methodology/Findings: We recruited 210 drug users in Shanwei city, Guangdong province. Among them, HCV RNA was detected in 150 (71.4%), both E1 and NS5B genes were sequenced in 136, and 6a genotyped in 70. Of the 6a sequences, most were grouped into three clusters while 23% represent emerging strains. For coalescent analysis, additional 6a sequences were determined among 21 blood donors from Vietnam, 22 donors from 12 provinces of China, and 36 IDUs from Liuzhou City in Guangxi Province. Phylogeographic analyses indicated that Vietnam could be the origin of 6a in China. The Guangxi Province, which borders Vietnam, could be the first region to accept 6a for circulation. Migration from Yunnan, which also borders Vietnam, might be equally important, but it was only detected among IDUs in limited regions. From Guangxi, 6a could have further spread to Guangdong, Yunnan, Hainan, and Hubei provinces. However, evidence showed that only in Guangdong has 6a become a local epidemic, making Guangdong the second source region to disseminate 6a to the other 12 provinces. With a rate of 2.737×10 -3 (95% CI: 1.792×10 -3 to 3.745×10 -3), a Bayesian Skyline Plot was portrayed. It revealed an exponential 6a growth during 1994-1998, while before and after 1994-1998 slow 6a growths were maintained. Concurrently, 1994-1998 corresponded to a period when contaminated blood transfusion was common, which caused many people being infected with HIV and HCV, until the Chinese government outlawed the use of paid blood donations in 1998. Conclusions/Significance: With an origin from Vietnam, 6a has become a local epidemic in Guangdong Province, where an increasing prevalence has subsequently led to 6a spread to many other regions of China. © 2012 Fu et al.


Zhang B.-Y.,Childrens Care Hospital of Guangdong Province | Wei Y.-S.,Guangzhou Medical College | Niu J.-M.,Childrens Care Hospital of Guangdong Province | Li Y.,Childrens Care Hospital of Guangdong Province | And 2 more authors.
International Journal of Gynecology and Obstetrics | Year: 2010

Objective: To determine risk factors for recurrent spontaneous abortion (RSA) in women from southern China. Method: We looked for associations between RSA and body mass index (BMI), family history of spontaneous abortion, smoking, exposure to environmental tobacco smoke (ETS [also known as passive smoking]), and alcohol and coffee consumption using an unconditional logistic regression model involving 326 patients with RSA and 400 controls. Results: Whereas smoking, alcohol consumption, and coffee consumption were not associated with increased risk of RSA, both short (< 1 hour/day) and long (≥ 1 hour/day) periods of ETS were associated (adjusted odds ratio [OR], 2.30; 95% confidence interval [CI], 1.50-3.52 and adjusted OR, 4.75; 95% CI, 3.23-6.99, respectively). The increased risk of RSA was significant for participants with a BMI of 24.0 or greater (adjusted OR, 1.54; 95% CI, 1.12-2.14) and those with a family history of miscarriage (adjusted OR, 2.12; 95% CI, 1.28-3.49). Conclusion: We found ETS, a higher BMI, and a family history of RSA to be independent risk factors for RSA in our population. © 2009 International Federation of Gynecology and Obstetrics.


Zhang Q.,Family Planning Research Institute of GuangDong Province | Zheng L.,Family Planning Research Institute of GuangDong Province | Tian P.,Family Planning Research Institute of GuangDong Province | Ye J.,Family Planning Research Institute of GuangDong Province | And 4 more authors.
Chinese Journal of Andrology | Year: 2010

Objective: To investigate the relationship between chromosomal abnormalities and oligozoospermia, severe oligozoospermia, asthenozoospermia, teratozoospermia as Well as azoospermia. Methods: Chromosome specimens were made from peripheral blood lymphocytes. Karyotyping analysis was conducted by G banding. Results: Of 567 patients with abnormal semen, 101 cases(17.8%,101/567) were detected to have abnormal chromosomes. And the rates of abnormal chromosome was 6.8% (6/88), 15.4% (2/13), 14.3% (3/21), 0%(0/l) and 20.3%(90/444) respectively in patients with oligozoospermia, severe oligozoospermia, asthenozoospermia, teratozoospermia and azoospermia. And 51 cases with polymorphic chromosomes (9.0%) were observed in 567 patients. Conclusion: Chromosomal abnormalities might be the major factor for spermatogenic arrest. Chromosome screening test was very important for diagnosis and treatment of patients with abnormal semen.


Zhang Q.,Family Planning Research Institute of GuangDong Province | Zheng L.,Family Planning Research Institute of GuangDong Province | Tian P.,Family Planning Research Institute of GuangDong Province | Ye J.,Family Planning Research Institute of GuangDong Province | And 4 more authors.
Chinese Journal of Andrology | Year: 2010

Objective: To investigate the effect of chromosomal analysis on sperm bank screening for preventing born baby with chromosomal abnormality after artificial insemination. Methods: Chromosome specimens were made using routine culture of peripheral blood lymphocytes. Karyotyping was analyzed by G banding stains. Results: Five cases with abnormal chromosomes (0.67%) were detected in 750 donors with qualified semen after primary screening test Polymorphism was found in 55 cases and the polymorphism rate was 7.33%. Conclusion: Abnormal chromosomes can be found in eligible donors who passed the primary semen screening test. Chromosome analysis was of great significance in preventing birth defects after artificial insemination.


Tang L.X.,Family Planning Research Institute of Guangdong Province | Wang Q.L.,Family Planning Research Institute of Guangdong Province | Tang Y.G.,Family Planning Research Institute of Guangdong Province | Jiang F.,Family Planning Research Institute of Guangdong Province | And 2 more authors.
Zhonghua nan ke xue = National journal of andrology | Year: 2013

To study the semen quality of confirmed fertile men with the time to pregnancy (TTP) of 12 months or less, and analyze the correlation of semen parameters with TTP. We recruited 1 152 men aged 18 -50 years and with TTP of 12 months. We used questionnaire investigation, physical examination and semen analysis, calculated the normal reference ranges of semen parameters, and analyzed the effects of TTP and other biological characteristics on semen quality. Seminal examinations showed that the semen volume was 1.5 ml, sperm concentration 17 million per ml, total sperm number 39 million per ejaculate, total sperm motility 41%, progressive sperm motility 33%, normal sperm morphology 5.0%, sperm vitality 53%, and total number of progressively motile sperm 17 million per ejaculate in the 1 152 subjects. In comparison, the total number of progressively motile sperm was significantly smaller in those aged > or = 35 years (P < 0.01), morphologically normal sperm were markedly less in those with BMI > 25.0 (P < 0.01), and sperm concentration, total number of sperm, morphologically normal sperm rate and total number of progressively motile sperm were remarkably lower in those with BMI < 18. 5 (P < 0.05). Cigarette smoking significantly reduced the sperm volume (P < 0.01), and drinking (> 10 g per week) decreased the sperm volume and the total numbers of sperm, morphologically normal sperm and progressively motile perm (P < 0.05). TTP was negatively correlated with sperm concentration, total number of sperm and total number of progressively motile sperm (P < 0.01). The subjects with sexual abstinence of 5 -7 days showed markedly higher sperm concentration, total number of sperm and total number of progressively motile sperm than others (P < 0.01). In normal fertile men, the fifth centile of semen parameters is close to the WHO normal reference range, TTP is negatively correlated with semen quality, and age, BMI and lifestyle may affect some of the semen parameters.


To explore predictive value of endometrial receptivity and pregnancy outcome by hysteroscopy examination at the phase of implantation window in unexplained infertile women. From Oct. 2007 to Mar. 2009, 93 unexplained infertile women underwent hysteroscopy examination at 7 approximately 9 days after a spontaneous ovulation in Family Planning Research Institute of Guangdong Province. According to the endometrial glandular openings and vascular shape, 79 cases without pathological endometrial changes were divided into 60 cases in good endometrium group and 19 cases in poor endometrium group. The following clinical parameters were analyzed and compared between two groups, including endometrial configuration, thickness, secretion, the development and number of pinopodes, vascular distribution, and the level of sex hormone, leukemia inhibitory factor (LIF) and glycodelin in the uterine flushing, and pregnancy outcome. (1) There was no statistical difference in the level of serum estrogen and progesterone at the phase of implantation window, which were (518 +/- 176) pmol/L, (40 +/- 20) nmol/L in good group and (513 +/- 244) pmol/L, (37 +/- 19) nmol/L in poor group (P < 0.05). The endometrium thickness at periovulatroy and implantation window days (1.06 +/- 0.10) cm/(1.16 +/- 0.08) cm in good group did not show significant difference with (0.93 +/- 0.12) cm/(1.02 +/- 0.10) cm in poor group (P > 0.05). The proportion of type A, B and C endometrium at periovulatory days were 63% (12/19), 37% (7/19) and 0 (0/19) in good group and 23% (14/60), 77% (46/60) and 0 (0/60) in poor group. When compared with those of type A or B between two groups respectively, it all showed statistical difference (P < 0.05). However, at phase of implantation window, endometrium configurations were all type B at both groups. (2) 90% (17/19) of women in good group and 7% (4/60) of women in poor group showed normal endometrial secretion function, which showed significant differences (P < 0.01). (3) The percentage of fully developed pinopodes and abundant pinopodes [84% (16/19) and 90% (17/19)] in good group were significantly higher than 42% (25/60) and 57% (34/60) in poor group (P < 0.05). (4) The level of CD(34) expression and microvessel density [MVD; (40.1 +/- 1.2) positive unit (PU) and (21.7 +/- 4.0)/high power field (HP)] in good group were significantly higher than (18.1 +/- 1.3) PU and (8.5 +/- 1.3)/HP in poor group (P < 0.01). (5) The level of LIF and glycodelin in uterine flushing [(72 +/- 54) ng/L and (196 +/- 20) microg/L] in good group were significantly higher than (15 +/- 16) ng/L and (116 +/- 26) microg/L in poor group (P < 0.05). (6) The rate of clinical pregnancy, spontaneous abortion and term delivery were 74% (14/19), 0 (0/14) and 100% (14/14) in good group and 23% (14/60), 14% (2/14) and 86% (12/14) in poor group, the rate of clinical pregnancy and term delivery in good group were significantly increased when compared with those in poor group (P < 0.01). Hysteroscopy examination at the phase of implantation window could reflect the development of glandular openings and vasculature. It is a preferable method to evaluate the endometrial receptivity and predict pregnancy outcome.


PubMed | Family Planning Research Institute of Guangdong Province
Type: Journal Article | Journal: Zhonghua nan ke xue = National journal of andrology | Year: 2014

To study the semen quality of confirmed fertile men with the time to pregnancy (TTP) of 12 months or less, and analyze the correlation of semen parameters with TTP.We recruited 1 152 men aged 18 -50 years and with TTP of 12 months. We used questionnaire investigation, physical examination and semen analysis, calculated the normal reference ranges of semen parameters, and analyzed the effects of TTP and other biological characteristics on semen quality.Seminal examinations showed that the semen volume was 1.5 ml, sperm concentration 17 million per ml, total sperm number 39 million per ejaculate, total sperm motility 41%, progressive sperm motility 33%, normal sperm morphology 5.0%, sperm vitality 53%, and total number of progressively motile sperm 17 million per ejaculate in the 1 152 subjects. In comparison, the total number of progressively motile sperm was significantly smaller in those aged > or = 35 years (P < 0.01), morphologically normal sperm were markedly less in those with BMI > 25.0 (P < 0.01), and sperm concentration, total number of sperm, morphologically normal sperm rate and total number of progressively motile sperm were remarkably lower in those with BMI < 18. 5 (P < 0.05). Cigarette smoking significantly reduced the sperm volume (P < 0.01), and drinking (> 10 g per week) decreased the sperm volume and the total numbers of sperm, morphologically normal sperm and progressively motile perm (P < 0.05). TTP was negatively correlated with sperm concentration, total number of sperm and total number of progressively motile sperm (P < 0.01). The subjects with sexual abstinence of 5 -7 days showed markedly higher sperm concentration, total number of sperm and total number of progressively motile sperm than others (P < 0.01).In normal fertile men, the fifth centile of semen parameters is close to the WHO normal reference range, TTP is negatively correlated with semen quality, and age, BMI and lifestyle may affect some of the semen parameters.

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