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São José do Rio Preto, Brazil

Bellini M.F.,Sao Paulo State University | Cury P.M.,FAMERP | Silva A.E.,Sao Paulo State University
Anticancer Research | Year: 2010

Background: The present study aimed to evaluate apoptosis and cell proliferation alterations in esophageal benign lesions in comparison to esophageal carcinomas. Materials and Methods: Immunohistochemistry was performed for caspase-3 protein (CPP32) and Ki-67 antigen expression in the esophageal mucosa from patients with Chagas disease (CD) with and without megaesophagus (CM), chronic esophagitis (CE), esophageal carcinoma (ESCC) and in normal mucosa (NM). Results: The Ki-67 labeling index (LI) was similar in all groups (range: 30%-48%), having no statistically significant difference among the groups. Positive CPP32 immunostaining was observed with similar frequency in the CD (30.8%), CM (30.4%) and CE (34.8%) groups, but it was increased in the ESCC group (555%); however, it was not statistically different from the other groups. No associations among the levels of CPP32 and Ki-67 expression were observed in the various groups, neither among parameters such as age, gender or alcohol and tobacco consumption. Conclusion: There were no evident changes in cell proliferation and apoptosis in benign lesions studied.


de Godoy J.M.P.,Medical School | de Godoy M.F.G.,FAMERP
International Journal of Medical Sciences | Year: 2010

The aim of this paper is to report new options in the treatment of lymphedema for under-privileged populations. Several articles and books have been published reporting recent advances and contributions. A new technique of manual lymph drainage, mechanisms of compression, development of active and passive exercising apparatuses and the adaptation of myolymphokinetic activities have been developed for the treatment of lymphedema. This novel approach can be adapted for the treatment of lymphedema in mass. © Ivyspring International Publisher.


Godoy M.F.G.,Medicine School in Sao Jose Do Rio Preto | Pereira M.R.,FAMERP
International Journal of Medical Sciences | Year: 2012

Trial design: A randomized controlled trial was performed to evaluate the effect of the combination of compression therapy with active exercising using a facilitating apparatus on arm lymphedema. Method: Twenty women with a mean age of 63.3 years were evaluated; all had lymphedema resulting from breast cancer treatment. The inclusion criterion was a dif-ference of 200 mL in size between arms. The apparatus used, called 'pulley system', is a ver-tical iron wheel fixed on a support at a distance of 10 cm from the patient's body. Participants were submitted to two series of active exercises using this facilitating device, one series using a compression sleeve and the other without. Each series consisted of four 12-minute sessions of exercises separated by 3-minute rest intervals. Volumetry was performed before and after each series of exercises. The paired t-test was utilized for statistical analysis (p-value < 0.05). Results: A significant mean reduction (p-value < 0.007) and non-significant mean increase (p-value < 0.2) in volumes were observed during exercising with and without compression, respectively. Conclusion: Controlled active exercising utilizing a facilitating apparatus while wearing a compression sleeve reduces the size of lymphedematous arms. © Ivyspring International Publisher.


Luna D.M.N.,Federal University of Pernambuco | Oliveira M.D.L.,Federal University of Pernambuco | Nogueira M.L.,FAMERP | Andrade C.A.S.,Federal University of Pernambuco
Chemistry and Physics of Lipids | Year: 2014

In this work, we developed a biosystem based on Concanavalin A (ConA) and lipid membranes to recognize glycoproteins from the serum of patients contaminated with dengue serotypes 1, 2 and 3 (DENV1, DENV2 and DENV3). The modified gold electrode was characterized using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and atomic force microscopy. Morphological analyses of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), DPPC-ConA, DPPC-ConA-DENV1, DPPC-ConA-DENV2 and DPPC-ConA-DENV3 revealed the existence of a non-uniform covering and large globules. EIS and CV measurements have shown that redox probe reactions on the modified gold electrodes were partially blocked due to the adsorption of lipid-ConA system and reveal the interaction response of the immobilized ConA to the presence of glycoproteins of dengue serum. The biosystem exhibited a wide linear response to different concentrations of sera of dengue serotypes 1, 2 and 3. A higher impedimetric response to glycoproteins present in dengue serotype 3 was observed. Our results demonstrate the applicability of lectin and lipid membranes to the development of biosensors for dengue infections. © 2014 Elsevier Ireland Ltd.


Fornitano L.D.,FAMERP | Godoy M.F.,FAMERP
Obesity Surgery | Year: 2010

Background Obesity is an independent risk factor for cardiovascular disease, especially in its morbid form. Bariatric surgery is an effective therapeutic option for sustained weight loss. However, there is no consensus in these cases regarding the performance of functional tests for preoperative evaluations. The aim of the present study was to analyze clinical, electrocardiographic, and hemodynamic response variables using a conventional exercise test on individuals with morbid obesity in comparison to overweight individuals. Methods Six hundred seventeen cases were included. Group I had 290 patients with morbid obesity and group II had 327 overweight patients. All were submitted to a conventional treadmill exercise test. Classic positivity criteria were used. Results Both groups tolerated the test well. There was no statistically significant difference in terms of positivity of the test [ST segment abnormality or angor (P=0.3863) or ST segment abnormality alone (P=0.1000)]. The significantly higher values in the obese individuals were basal heart rate, basal systolic arterial pressure (SAP), maximum SAP, basal diastolic arterial pressure (DAP), maximum DAP, chronotropic deficit (P<0.0001), and maximum ratepressure product (P<0.0421). The significantly lower values in the obese individuals were exercise time, maximum heart rate, and metabolic equivalents values (P<0.0001). Conclusion Hemodynamic parameters were significantly different between the two groups. However, there were no differences in terms of ST segment abnormalities or effortinduced angina. The very low functional capacity and complex arrhythmia found in some obese individuals draws attention to the need for complementary investigation, with the aim of diminishing the risk of postoperative heart complications. © 2008 Springer Science + Business Media, LLC.

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