Faith Biomedical Engineering

MI, United States

Faith Biomedical Engineering

MI, United States
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Ibrahim M.A.,Royal Scientific Society | Saleh N.,University of Baghdad | Archoukieh E.,National Commission for Biotechnology | Al-Obaide H.W.,Faith Biomedical Engineering | And 2 more authors.
International Journal of Cancer Research | Year: 2010

Molecular genetic studies suggested that genetic instabilities of genomic DNA are implicated in the pathogenesis of cancers. In this study the molecular genomic polymorphism of acute lymphoblastic leukemia was analyzed by 23 arbitrary primers of decamer oligonucleotides to investigate the genetic correlation by cluster tree analysis. Ten primers were able to amplify genomic DNA of leukemia patients, but varied in their ability to detect genomic DNA instability. One of amplifying primers showed novel pattern of amplified DNA fragments. It amplified DNA fragment (3.162 kbp) in normal individuals but was absent in 77.8% of patients, whereas other amplified DNA fragment (0.98 kbp) was absent in normal individuals but it was present in 55.6% of patients. Moreover, dissimilarity matrix analysis of amplified DNA fragments provided genetic relatedness among genomic DNA of patients. © 2010 Academic Journals Inc.


Saleh N.,Nahrain University | Ibrahim M.A.,Royal Scientific Society | Archoukieh E.,National Commission for Biotechnology | Makkiya A.,Qatar University | And 2 more authors.
Biotechnology | Year: 2010

The aim of this study is to ascertain the possible application of Random Amplification of Polymorphic DNA (RAPD) analysis as a genetic test to investigate DNA polymorphisms and detection of genomic markers in various types of leukemia. The results showed unique profiles of amplified DNA fragments produced in genomic DNA of three types of leukemia by an arbitrary primer of decamer oligonucleotides OPA-09. The primer produced four types of amplified DNA fragments (980, 1659, 2187 and 3162 bp). The smallest amplified DNA fragment (980 bp) appeared in 14.3 and 13.3% of tested acute myeloid leukemia and chronic myeloid leukemia patients, respectively; but was absent in genomic DNA of chronic lymphoid leukemia and normal individuals. Whereas the largest amplified fragment (3162 bp) was present in 12.5, 20 and 75% of chronic lymphoid leukemia, chronic myeloid leukemia and normal individuals, respectively and was absent in acute myeloid leukemia. On the other hand, the two amplified fragments (1659 and 2187 bp) were present in normal and leukemia patients. Cluster analysis of amplified DNA fragments grouped the leukemia patients in two main groups. The detected DNA polymorphisms by the arbitrary primer OPA-09 might find application in developing efficient RAPD primer for diagnosis of leukemia. © 2010 Asian Network for Scientific Information.

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