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Brno, Czech Republic

Ocadlikova D.,Masaryk University | Ocadlikova D.,Laboratory of Experimental Hematology and Cellular Immunotherapy | Kryukov F.,Masaryk University | Kryukov F.,Laboratory of Experimental Hematology and Cellular Immunotherapy | And 11 more authors.
Neoplasma | Year: 2010

Dendritic cells are able to induce anti-tumor immune responses by presenting tumor-specific antigens to T-lymphocytes. Various tumor-associated antigens have been studied in multiple myeloma in an effort to find a strong antigen capable of generating clinically meaningful responses in vaccinated patients. The aim of our study was to generate myeloma-specific cytotoxic T lymphocytes in vitro using dendritic cells loaded with peptide antigens or apoptotic bodies. Peripheral blood mononuclear cells from HLA-A2+ healthy donors were used for isolation and culture of dendritic cells (DCs) and T lymphocytes. DCs were loaded with hTERT- and MUC1-derived nonapeptides or apoptotic bodies from myeloma cells. Repeated stimulation of T lymphocytes led to their activation characterized by interferon-gamma production. Activated T lymphocytes were separated immunomagnetically and expanded in vitro. Specific cytotoxicity of the expanded T lymphocytes was tested against a myeloma cell line. There was evidence of cytotoxicity for all three types of antigens used for T lymphocyte priming and expansion. No statistically significant differences were observed in T lymphocyte cytotoxicity for any of the antigens. We present a method for the priming and expansion of myeloma-specific T lymphocytes using dendritic cells loaded with different types of tumor antigens. Cytotoxic T lymphocytes and/or activated dendritic cells generated by the described methods can be applied for cellular immunotherapy against multiple myeloma and other malignancies.


Preiss M.,Prague Psychiatric Center | Kucerova H.,The Faculty Hospital Brno | Lukavsky J.,Academy of Sciences of the Czech Republic | Sos P.,Prague Psychiatric Center | And 2 more authors.
European Journal of Psychiatry | Year: 2010

Background and Objectives: Little is known about the differences between patients managing depression on an outpatient basis as compared with hospitalized ones. This study investigated the performance of attention, executive function and verbal memory during remission from unipolar depressive episodes and compare patients with and without history of hospitalization. Methods: The sample of participants who had undergone one or more hospitalizations (hospitalized, N = 46) as well as in a sample without hospitalization (never hospitalized, N = 46) and controls (N = 92) were used. The Auditory Verbal Learning Test (AVLT) and the Trail Making Test (TMT) were administrated to test this hypothesis. Results and conclusion: The hospitalized sample had similar results in all four neuropsychological variables in comparison with the never hospitalized group, and both groups had some lower results in comparison with controls. In comparison with the controls, hospitalized sample had mean cognitive deficits of 34% (28-41%), the never hospitalized group had a mean of 20% (21-35%). Contrary to previous reports we have found no meaningful differences between the two patient groups.


Preiss M.,Prague Psychiatric Center | Kramska L.,Na Homolce Hospital | Dockalova E.,Prague Psychiatric Center | Holubova M.,Prague Psychiatric Center | Kucerova H.,The Faculty Hospital Brno
European Psychiatry | Year: 2010

Background: The capacity to focus and concentrate or to direct attention supports many aspects of cognitive functioning including short-term memory and higher-level cognitive functions. The purpose was to assess attentional networks in euthymic patients with unipolar depression using the Attentional Network Test (ANT). Materials and methods: We investigated performance of attention by virtue of ANT during remission from unipolar depressive disorder and tested a hypothesis that there are no differences between outpatient group (euthymic patients, N = 32) and matched controls in attentional variables, the relationship of attentional networks and everyday cognitive failures. Results: No differences between the groups in attentional networks were found and no relationship between attentional networks and cognitive failures was found. Limitations: One assessment during remission could be insufficient to recognize long-term pattern of cognitive functions. Conclusions: These data show non-impaired attentional networks possibly explained by sufficient level of remission and ameliorated influence of high education on cognition. © 2009 Elsevier Masson SAS. All rights reserved.


Bilik A.,The Faculty Hospital Brno | Krticka M.,The Faculty Hospital Brno | Kvasnicka P.,The Faculty Hospital Brno
Bratislava Medical Journal | Year: 2012

Background and aims: The most common mechanisms of the knee injuries are distorsions and contusions. Fairly often they will result in hemarthrosis. The purpose of this study was to evaluace the fi ndings in patiens with acute traumatic hemarthrosis of the knee and based on these fi ndings estimate the necessity of acute artroscopic examination with therapeutic procedur. Material and methods: In the years 2007 and 2009 a total of 266 patients underwent acute arthroscopic examination in Department of Traumatology the Faculty Hospital Brno after the acute knee trauma. Patiens with other intraarticular bone fractures were excluded. Results: The ruptures of the ACL were the most common fi ndings (52 %), meniscal tear (27 %), dislocation of the patella(11 %) and chondral or subchondral fractures were (13 %). In 224 (84 %) cases was performed artroscopic therapeutic procedure and in only 43 (16 %) case was performed routine arthroscopic examination. Conclusions: Based on our fi ndings we believe that arthroscopic examination is necessary in patiens with acute traumatic haemarthrosis of the knee and it is better methods like NMR examination because is provided to execute therapeutic procedure.

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