Facultat de Farmacia

Barcelona, Spain

Facultat de Farmacia

Barcelona, Spain
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Suarez-Germa C.,Facultat de Farmacia | Suarez-Germa C.,University of Barcelona | Loura L.M.S.,University of Coimbra | Prieto M.,University of Lisbon | And 7 more authors.
Journal of Physical Chemistry B | Year: 2013

Förster resonance energy transfer (FRET) measurements were performed in preceding works to study the selectivity between a single-tryptophan mutant of lactose permease (LacY) of Escherichia coli (used as the donor) and phospholipid probes labeled with pyrene at the acyl chain moiety (used as the acceptor). In the present work, we report the results obtained by using the same LacY mutant (W151/C154G) and binary lipid mixtures of phosphatidylethanolamine (PE) differing in the acyl chain composition and 1-palmitoyl-2-oleoyl-sn- glycero-3-[phospho-rac-(1-glycerol)] (POPG) (3:1 mol/mol) doped with a phospholipid probe labeled with pyrene at the headgroup. The use of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(1-pyrenesulfonyl) ammonium salt (HPyr-PE), which bears two unsaturated acyl chains, enabled the investigation of the specific interaction between LacY and HPyr-PE. The main conclusions raised from our results suggest that (i) for phase-separated systems, LacY would be located in fluid domains nominally enriched in POPG, and if a given proportion of PE is present in this phase, it will be mainly located around LacY; and (ii) in the absence of phase separation, LacY is preferentially surrounded by PE and, in particular, seems to be sensitive to the lipid spontaneous curvature. © 2013 American Chemical Society.


Del Valle J.,Facultat de Farmacia | Del Valle J.,CIBER ISCIII | Duran-Vilaregut J.,Facultat de Farmacia | Duran-Vilaregut J.,CIBER ISCIII | And 9 more authors.
Neurodegenerative Diseases | Year: 2011

Cerebrovascular dysfunction and β-amyloid peptide deposition on the walls of cerebral blood vessels might be an early event in the development of Alzheimer's disease. Here we studied the time course of amyloid deposition in blood vessels and blood-brain barrier (BBB) disruption in the CA1 subzone of the hippocampus of SAMP8 mice and the association between these two variables. We also studied the association between the amyloid deposition in blood vessels and the recently described amyloid clusters in the parenchyma, as well as the association of these clusters with vessels in which the BBB is disrupted. SAMP8 mice showed greater amyloid deposition in blood vessels than age-matched ICR-CD1 control mice. Moreover, at 12 months of age the number of vessels with a disrupted BBB had increased in both strains, especially SAMP8 animals. At this age, all the vessels with amyloid deposition showed BBB disruption, but several capillaries with an altered BBB showed no amyloid on their walls. Moreover, amyloid clusters showed no spatial association with vessels with amyloid deposition, nor with vessels in which the BBB had been disrupted. Finally, we can conclude that vascular amyloid deposition seems to induce BBB alterations, but BBB disruption may also be due to other factors. © 2011 S. Karger AG, Basel.


Suarez-Germa C.,Facultat de Farmacia | Suarez-Germa C.,Institute Of Nanociencia I Nanotecnologia In2 | Hernandez-Borrell J.,Facultat de Farmacia | Hernandez-Borrell J.,Institute Of Nanociencia I Nanotecnologia In2 | And 2 more authors.
Molecular Membrane Biology | Year: 2014

Förster resonance energy transfer (FRET) is a photophysical process by which a donor (D) molecule in an electronic excited state transfers its excitation energy to a second species, the acceptor (A). Since FRET efficiency depends on D-A separation, the measurement of donor fluorescence in presence and absence of the acceptor allows determination of this distance, and therefore FRET has been extensively used as a "spectroscopic ruler". In membranes, interpretation of FRET is more complex, since one D may be surrounded by many A molecules. Such is the case encountered with membrane proteins and lipids in the bilayer. This paper reviews the application of a model built to analyze FRET data between a single tryptophan mutant of the transmembrane protein lactose permease (W151/C154G of LacY), the sugar/H+ symporter from Escherichia coli, and different pyrene-labeled phospholipids. Several variables of the system with biological implication have been investigated: The selectivity of LacY for different species of phospholipids, the enhancement of the sensitivity of the FRET modeling, and the mutation of a particular aminoacid (D68C) of the protein. The results obtained support: (i) Preference of LacY for phosphatidylethanolamine (PE) over phosphatidylglycerol (PG); (ii) affinity of LacY for fluid (Lα) phases; and (iii) importance of the aspartic acid in position 68 in the sequence of LacY regarding the interaction with the phospholipid environment. Besides, by exploring the enhancement of the sensitivity by using pure lipid matrices with higher mole fractions of labelled-phospholipid, the dependence on acyl chain composition is unveiled. © 2014 Informa UK Ltd.


Lizarraga D.,University of Barcelona | Lizarraga D.,Maastricht University | Vinardell M.P.,Facultat de Farmacia | Noe V.,University of Barcelona | And 9 more authors.
Journal of Nutrition | Year: 2011

Diet plays a decisive role in promoting or preventing colon cancer. However, the specific effects of some nutrients remain unclear. The capacity of fruit and vegetables to prevent cancer has been associated with their fiber and antioxidant composition. We investigated whether consumption of a lyophilized red grape pomace containing proanthocyanidin-rich dietary fiber (grape antioxidant dietary fiber, GADF) by female C57BL/6J mice would affect the serum metabolic profile or colonmucosa gene expression using NMR techniques and DNA microarray, respectively. Themice were randomly assigned to 2 groups that for 2 wk consumed a standard rodent diet andwere gavagedwith 100mg/kg body weight GADF suspended in water or an equivalent volume of plain tap water (10 mL/kg body weight). The amount of fiber supplemented was calculated to equal the current recommended daily levels of fiber consumption for humans. The inclusion of dietary GADF induced alterations in the expression of tumor suppressor genes and proto-oncogenes as well as themodulation of genes from pathways, including lipid biosynthesis, energymetabolism, cell cycle, and apoptosis. Overexpression of enzymes pertaining to the xenobiotic detoxifying system and endogenous antioxidant cell defenses was also observed. In summary, the genetic and metabolic profiles induced by GADF were consistent with the preventive effects of fiber and polyphenols. On the basis of these observations,we propose that GADF may contribute to reducing the risk of colon cancer. © 2011 American Society for Nutrition.


Picas L.,Facultat de Farmacia | Montero M.T.,Facultat de Farmacia | Morros A.,Unitat de Biofisica | Morros A.,Center dEstudis en Biofisica | And 2 more authors.
Biochimica et Biophysica Acta - Biomembranes | Year: 2010

Biochemical and structural work has revealed the importance of phospholipids in biogenesis, folding and functional modulation of membrane proteins. Therefore, the nature of protein-phospholipid interaction is critical to understand such processes. Here, we have studied the interaction of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and 1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (POPG) mixtures with the lactose permease (LacY), the sugar/H + symporter from Escherichia coli and a well characterized membrane transport protein. FRET measurements between single-W151/C154G LacY reconstituted in a lipid mixture composed of POPE and POPG at different molar ratios and pyrene-labeled PE or PG revealed a different phospholipid distribution between the annular region of LacY and the bulk lipid phase. Results also showed that both PE and PG can be part of the annular region, being PE the predominant when the PE:PG molar ratio mimics the membrane of E. coli. Furthermore, changes in the thermotropic behavior of phospholipids located in this annular region confirm that the interaction between LacY and PE is stronger than that of LacY and PG. Since PE is a proton donor, the results obtained here are discussed in the context of the transport mechanism of LacY. © 2009 Elsevier B.V. All rights reserved.


Rana V.S.,Institute of Bio Resources of Sustainable Development | Blazquez M.A.,Facultat de Farmacia
Journal of Essential Oil Research | Year: 2010

The chemical composition of the volatile oil of Zanthoxylum rhetsa seed coat was analyzed by GC and GC/MS. Thirty-four compounds, accounting for 87.4% of the oil were identified. The major compounds were terpinen-4-ol (32.1%), α-terpineol (8.2%), sabinene (8.1%), β-phellandrene (7.4%) and 2-undecanone (7.1%). © 2010 Allured Business Media.


Rodriguez-Lagunas M.J.,Facultat de Farmacia | Ferrer R.,Facultat de Farmacia | Moreno J.J.,Facultat de Farmacia
Prostaglandins Leukotrienes and Essential Fatty Acids | Year: 2013

Prostaglandins (PG) are inflammatory mediators derived from arachidonic or eicosapentaenoic acid giving rise to the 2-series or the 3-series prostanoids, respectively. Previously, we have observed that PGE2 disrupts epithelial barrier function. Considering the beneficial effect of fish oil consumption in intestinal inflammatory processes, the aim of this study was to assess the role of PGE3 on epithelial barrier function assessed from transepithelial electrical resistance and dextran fluxes in Caco-2 cells. The results indicate that PGE3 increased paracellular permeability (PP) to the same extent as PGE2, through the interaction with EP1 and EP4 receptors and with intracellular Ca2+ and cAMP as the downstream targets. Moreover, we observed a redistribution of tight junction proteins, occludin and claudin-4. In conclusion, PGE3 is able to increase PP thus leading to reconsider the role of PGE2/PGE3 ratio in the beneficial effects of dietary fish oil supplementation in the disruption of barrier function. © 2013 Elsevier Ltd.


PubMed | Facultat de Farmacia
Type: Journal Article | Journal: Toxicology in vitro : an international journal published in association with BIBRA | Year: 2010

The ocular irritancy potential of different substances used as vehicles has been tested by the Draize test method and the alternative hens egg test-chorioallantoic membrane (HET-CAM) test. With the latter test, carboxymethylcellulose was not found to be irritant and can be used to suspend insoluble substances to be assessed by this method. The ocular irritancy potential of six commercial disinfectants was also tested by the Draize test method and the alternative HET-CAM test. Products were assigned to categories of irritancy, according to two different sets of criteria for the Draize test results, and according to irritancy potential assessed by the HET-CAM test. When both sets of results for Draize categories were compared with those for the HET-CAM test, four of the six products tested showed similar irritancy potential. The remaining two products gave false positive results on the HET-CAM compared with the Draize test.


PubMed | Facultat de Farmacia
Type: Journal Article | Journal: Neuro-degenerative diseases | Year: 2011

Cerebrovascular dysfunction and -amyloid peptide deposition on the walls of cerebral blood vessels might be an early event in the development of Alzheimers disease. Here we studied the time course of amyloid deposition in blood vessels and blood-brain barrier (BBB) disruption in the CA1 subzone of the hippocampus of SAMP8 mice and the association between these two variables. We also studied the association between the amyloid deposition in blood vessels and the recently described amyloid clusters in the parenchyma, as well as the association of these clusters with vessels in which the BBB is disrupted. SAMP8 mice showed greater amyloid deposition in blood vessels than age-matched ICR-CD1 control mice. Moreover, at 12 months of age the number of vessels with a disrupted BBB had increased in both strains, especially SAMP8 animals. At this age, all the vessels with amyloid deposition showed BBB disruption, but several capillaries with an altered BBB showed no amyloid on their walls. Moreover, amyloid clusters showed no spatial association with vessels with amyloid deposition, nor with vessels in which the BBB had been disrupted. Finally, we can conclude that vascular amyloid deposition seems to induce BBB alterations, but BBB disruption may also be due to other factors.


PubMed | Facultat de Farmacia
Type: Journal Article | Journal: Prostaglandins, leukotrienes, and essential fatty acids | Year: 2013

Prostaglandins (PG) are inflammatory mediators derived from arachidonic or eicosapentaenoic acid giving rise to the 2-series or the 3-series prostanoids, respectively. Previously, we have observed that PGE2 disrupts epithelial barrier function. Considering the beneficial effect of fish oil consumption in intestinal inflammatory processes, the aim of this study was to assess the role of PGE3 on epithelial barrier function assessed from transepithelial electrical resistance and dextran fluxes in Caco-2 cells. The results indicate that PGE3 increased paracellular permeability (PP) to the same extent as PGE2, through the interaction with EP1 and EP4 receptors and with intracellular Ca(2+) and cAMP as the downstream targets. Moreover, we observed a redistribution of tight junction proteins, occludin and claudin-4. In conclusion, PGE3 is able to increase PP thus leading to reconsider the role of PGE2/PGE3 ratio in the beneficial effects of dietary fish oil supplementation in the disruption of barrier function.

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