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Juazeiro do Norte, Brazil

Tavares S.M.Q.M.C.,Faculdade Leao Sampaio FALS | Bravo W.L.,Laboratorio Of Analises Clinicas Vicente Lemos | Lopes Silva M.R.,Laboratorio Of Analises Clinicas Vicente Lemos
Revista Brasileira de Hematologia e Hemoterapia | Year: 2014

OBJECTIVE: The aim of this work was to evaluate the lymphocyte immunophenotype in an elderly population. METHODS: This study enrolled 35 over 60-year-old volunteers and a control group composed of 35 young adults. The study included elderly without diseases that might affect the functioning of the immune system. These individuals were consulted by doctors and after a physical examination, laboratory tests were performed using a Beckman Coulter® flow cytometer. The GraphPad Prism computer program was employed for statistical analysis with the level of significance being set for p-values <0.05. RESULTS: There is a statistically significant reduction in the number of lymphocytes (CD8+, CD2+ and CD3+ cells) in the elderly compared to young adults. These low rates are explained by changes attributed to aging and may be partly responsible for the reduction in the cellular immune response, lower proliferative activity and the low cytotoxicity of lymphocytes. CONCLUSION: These parameters showed greater impairment of adaptive immunity in the elderly population and can therefore explain the greater fragility of the aged body to developing diseases. © 2014 Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier Editora Ltda. All rights reserved. Source


Pelegrini A.,Santa Catarina State University | Silva D.A.S.,Federal University of Santa Catarina | Silva J.M.F.D.L.,Faculdade Leao Sampaio FALS | Grigollo L.,University of West Santa Catarina | Petroski E.L.,Federal University of Santa Catarina
Revista Paulista de Pediatria | Year: 2015

Objective To determine the anthropometric indicators of obesity in the prediction of high body fat in adolescents from a Brazilian State. Methods The study included 1,197 adolescents (15-17 years old). The following anthropometric measurements were collected: body mass (weight and height), waist circumference and skinfolds (triceps and medial calf). The anthropometric indicators analyzed were: body mass index (BMI), waist circumference (WC), waist-to-height ratio (WHtR) and conicity index (C-Index). Body fat percentage, estimated by the Slaughter et al equation, was used as the reference method. Descriptive statistics, U Mann-Whitney test, and ROC curve were used for data analysis. Results Of the four anthropometric indicators studied, BMI, WHtR and WC had the largest areas under the ROC curve in relation to relative high body fat in both genders. The cutoffs for boys and girls, respectively, associated with high body fat were BMI 22.7 and 20.1 kg/m2, WHtR 0.43 and 0.41, WC 75.7 and 67.7 cm and C-Index 1.12 and 1.06. Conclusions Anthropometric indicators can be used in screening for identification of body fat in adolescents, because they are simple, have low cost and are non-invasive. © 2014 Associação de Pediatria de São Paulo. Source


Matias E.F.F.,Faculdade Leao Sampaio FALS | Alves E.F.,Faculdade Leao Sampaio FALS | Silva M.K.N.,Faculdade Leao Sampaio FALS | Carvalho V.R.A.,Faculdade Leao Sampaio FALS | And 9 more authors.
Microbial Pathogenesis | Year: 2016

Medicinal plants are often the only therapeutic resource for many communities and ethnic groups. Cordia verbenacea DC., "Erva-baleeira," is one of the species of plants currently used to produce a phytotherapeutic product extracted from its leaves. The present study aimed to establish its chemical profile, antibacterial activity and resistance-modulating potential. The C. verbenacea extracts were prepared from fresh leaves using solvents as methanol and hexane. Ethyl Acetate was used for the preparation of the fraction. Phytochemical screening was carried out using HPLC-DAD for determination and quantification of the secondary metabolites present in the fractions. Antibacterial and resistance-modulation assays were performed to determine minimum inhibitory concentration (MIC) using a microdilution assay. The data were subjected to statistical analysis with two-way ANOVA and Bonferroni posttests. Results of phytochemical prospecting and HPLC analysis of the fractions were in agreement with the literature. The natural products presented moderate antibacterial activity when considering the clinical relevance of a MIC of 256 μg/mL against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, and 512 μg/mL against P. aeruginosa. However, when the fractions were combined with antibiotics we observed a synergic effect, as natural products enhanced the antibacterial effect of aminoglycosides, significantly decreasing the MIC of antibiotics at 12.5%-98.4%. We believe that the data obtained from phytochemical analysis and from antibacterial and resistance modulation assays of C. verbenacea extracts new can open perspectives in the search for new alternatives for the treatment of bacterial infections and stimulate the renewed use of antibiotics with reduced effectiveness due to resistance. © 2016 Elsevier Ltd. Source

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