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Cheng Y.,Xian No.1 Hospital | Wu J.,Xian No.1 Hospital | Zhu H.-F.,Xian No.1 Hospital | Gao W.,Xian No.1 Hospital | And 2 more authors.
International Eye Science | Year: 2012

AIM: To quantitatively detect the diseased corneal epithelial cells, conjunctival cells before and after aqueous tear deficiency dry eye treatment by using corneal confocal microscope and conjunctival impression cytology, so as to further accurately evaluate the dry eye treatment outcome and prognosis. METHODS: There were 21 cases (41 eyes) which were clinically diagnosed as aqueous tear deficiency dry eye based on their history, symptoms, corneal and conjunctival signs and relevant examinations. All patients with dry eye were treated by polyethylene glycol eye drops combined with Carbopol gel. Based on conjunctival impression cytoscopy, squamous metaplasia and goblet cells number of conjunctival cells in dry eye patients were graded and the impression cells classification before and after treatment was statistically compared. Morphological change of nerve fibers under corneal epithelial before and after dry eye treatment was observed by using Heidelberg laser confocal microscopy. The changes of corneal epithelium cell density before and after treatment under peer conjunctival cells dryness were quantitatively evaluated. RESULTS: Impression cytoscopy: before treatment ≤ grade II in 6 eyes, grade III in 25 eyes, ≥grade IV in 10 eyes; 6 months after treatment ≤ grade II in 19 eyes(46.3%), grade III in 17 eyes(41.5%), ≥grade IV in 5 eyes(12.2%). The proportion of patients with different levels of impression cells before and after treatment was significantly changed. Morphological changes under confocal microscopy: cell density decreased in cell necrosis exfoliated area of corneal epithelial before treatment; corneal subcutaneous nerve fibers appeared branch unorganized, abnormal strike line, no subcutaneous nerve fibers in 5 eyes. Regional cell density increased 6 months after treatment. All patients can seen subcutaneous nerve fibers, a small number of cases still appeared branch unorganized, abnormal strike line. Confocal microscopy showed under the conjunctival impression cytology degree at the same level, the corneal epithelial cell density before and after treatment were compared, there was statistically significant difference (P<0.05). The cell density was significantly increased after treatment. CONCLUSION: The aqueous tear deficiency dry eye treatment outcomes were quantitatively observed by using corneal confocal microscopy and conjunctival impression cytology in order to determine the significant value of above two methods in dry eye treatment evaluation. Source


Zhang H.,Xian Jiaotong University | Zhang H.,Eye Institute of Shaanxi Province | Sun N.,Xian Jiaotong University | Liang H.,Eye Institute of Shaanxi Province | And 3 more authors.
Journal of Biomedical Research | Year: 2010

Objective: Retinopathy of prematurity is becoming obvious with the improvement of neonatal ambulance. However there is still not a good treatment. The present study is to observe the effect of 17 beta-estradiol (E2) on oxygen-induced retinopathy (OIR), and explore the relationship between the changes of avascular area and malondialdehyde (MDA) in retina. Methods: Newborn oxygen-exposed mice underwent subcutaneous injections of different dose of E2 (0.1 μg, 1.0 μg, 10.0 μg), tamoxifen or phosphate buffered saline (PBS; controls) everyday from post-natal day (p)7 to p17. At p17, retinal flat mounts were scored for the percentage of avascular/total retinal area, and pathological changes during revascularization. The MDA concentration in the retina was determined also. In the most efficacious E2 group (10.0 μg), 100.0 μg tamoxifen was also administered, and the percentage of capillary-free/total retinal area determined, and the retinal malondialdehyde concentration assayed. Results: The mean percentage of capillary-free area over total retinal area was 0(PBS, in room air), 34.197±1.301(PBS, in hyperoxia), 23.685±0.407 (0.1 μg E2), 14.648±0.355 (1.0 μg E2), 4.693±0.450 (10.0 μg E2) and 32.240±0.654 (10.0 μg E2 +100.0 μg tamoxifen). The difference was significant (F = 2778.759, P < 0.01), and the difference between any two groups were also significant (all P value were less than 0.01). The predilection of tufts and clusters during revascularization was mainly aggregated in zones 2 and 3, but the difference of retinal neovascular clusters and tufts in fourth zone among different groups were significant [clusters (F = 44.719, P < 0.01) vs tufts (F = 39.997, P < 0.01)]. The mean MDA concentration were 0.711±0.037(PBS, in room air), 2.084±0.066 (PBS, in hyperoxia), 1.829±0.091(0.1 μg E2), 1.152±0.067(1.0 μg E2), 0.796±0.027(10.0 μg E2), 1.988±0.049(10.0 μg E2 +100.0 μg tamoxifen) (F = 628.103, P < 0.01). The difference between any two groups were also significant (all P value were less than 0.05). The close relation between the percentage of avascular/total retinal area and MDA concentration was also verified (r = 0.981, P < 0.01). Conclusion: Oxidative stress responses play a pivotal role in OIR, by means of receptor pathway. E2 can alleviate oxidative stress reaction, and thus ameliorate the severity of oxygen induced retinopathy. © 2010 The Editorial Board of Journal of Biomedical Research. Source

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