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Nizza S.,University of Naples Federico II | Mallardo K.,University of Naples Federico II | Manco E.,University of Naples Federico II | Marullo A.,University of Naples Federico II | And 4 more authors.
Revista Veterinaria | Year: 2010

Lethal episodes in buffalo calf due to rotavirus and E. coli 0157:H7 is herein reported. Eight 30-day-old buffalo calves with severe diarrhoea were studied by bacteriological and virological examinations on their faecal samples. Episodes with rotavirus alone were more less severe as compared to episodes where co-infection with E. coli was found. The group A rotavirus isolates were characterized for G- and P-type and the E. coli strains were analysed by Vero cell assay and PCR. This study shows the presence of group A rotavirus of G6-P[5] serotypes and E. coli 0157:H7 (EHEC) in faecal samples. So far the concomitant presence of rotavirus and E. coli 0157:H7 in cases of enteritis in Bubalus bubalis in Italy is not reported in the literature. Diarrhoeal diseases caused by the above pathogens mostly affect young children, and those who live in rural areas usually have more infection from animals than those of urban dwellers. Our data might indicate a potential risk factor existing for the farm workers in buffalo-breedings in Italy. Source

Ferrante M.C.,University of Naples Federico II | Clausi M.T.,University of Naples Federico II | Naccari C.,Messina University | Fusco G.,Experimental Zooprophylactic Institute of Southern Italy | And 3 more authors.
Bulletin of Environmental Contamination and Toxicology | Year: 2014

Several polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) investigated in soft tissues of the frequently monitored Mytilus galloprovincialis were compared to those of Ensis siliqua, a highly dispersed and economically important bivalve species, though rarely investigated. Overall PCBs had higher concentrations than OCPs in both species with a prevalence of tri-tetra-And penta-chlorinated biphenyls in E. siliqua and a prevalence of hexa-hepta and octa-chlorinated biphenyls in M. galloprovincialis. E. siliqua emerges as a suitable complement to mussels for monitoring PCBs and OCPs pollution. © Springer Science+Business Media New York 2014. Source

Montagnaro S.,University of Naples Federico II | Sasso S.,University of Naples Federico II | De Martino L.,University of Naples Federico II | Longo M.,University of Naples Federico II | And 6 more authors.
Journal of Wildlife Diseases | Year: 2010

Serum samples were collected from wild boars (Sus scrofa) harvested during the 2005-2006 hunting season in Campania, southern Italy. Samples were tested for antibodies to Leptospira interrogan, Brucella spp., Salmonella spp., Aujeszky disease virus (ADV), porcine reproductive and respiratory stress syndrome virus (PRRSV), porcine parvovirus (PPV), classical swine fever virus (CSFV), and swine vesicular disease virus (SVDV). Of the 342 serum samples tested, 1.5 (4.4%) were seropositive to Brucella spp., nine (2.6%) were seropositive to L. interrogans, 66 (19.3%) were seropositive for Salmonella spp., 105 (30.7%) were seropositive for ADV, 27 (7.9%) were seropositive for PPV, and 129 (37.7%) were seropositive for PRRSV. All sera tested seronegative for SVDV and CSFV antibodies. These results, recorded for the first time in Campania, support the hypothesis that wild boar are reservoirs of certain infectious agents, but some infections in wild boars originate from their domestic counterparts. © Wildlife Disease Association 2010. Source

Fusco G.,Experimental Zooprophylactic Institute of Southern Italy | Amoroso M.G.,Experimental Zooprophylactic Institute of Southern Italy | Gesualdi Montesano N.,Qiagen | Viscardi M.,Experimental Zooprophylactic Institute of Southern Italy
MethodsX | Year: 2015

Identification of herpesvirus in biological material is usually carried out by real-time PCR. With the aim to classify the strain of virus identified, real-time PCR must be often supported by time-consuming capillary electrophoresis sequencing analysis. Here we provide a protocol for the rapid and reliable identification of 5 closely related herpesviruses by PyroMark Q24 sequencing system. PyroMark performs DNA sequencing analysis using pyrosequencing, a technology based on the detection of released pyrophosphate during DNA elongation [1]. PyroMark is designed to detect changes in specified variable positions of the DNA. It can efficiently detect single nucleotide differences in sequences [2]. In the present paper we describe a protocol to pyrosequence a small polymorphic segment of the US8 gene. On the basis of the differences identified in the nucleotide sequence we could readily classify the herpesvirus as Bovine herpesvirus 1.1, Bovine herpesvirus 1.2, Bovine herpesvirus 5, Bubaline herpesvirus 1 or Caprine herpesvirus. The protocol set up offers several advantages with respect to the techniques commonly used: it requires less than one working day to be carried;it gives the possibility to analyze, at reasonable costs, up to 24 samples at a time; andit allows to detect with great reliability and specificity strongly genetically correlated organisms like the herpesviruses named above. The procedure can be easily applied to other families of viruses, with opportune modifications. © 2015 The Authors. Source

Dore S.,National Reference Center for Sheep and Goat Mastitis Experimental Zooprophylactic Institute of Sardinia | Liciardi M.,National Reference Center for Sheep and Goat Mastitis Experimental Zooprophylactic Institute of Sardinia | Amatiste S.,National Reference Center for Ovine and Caprine Milk and Dairy Products Quality Experimental Zooprophylactic Institute of Lazio and Tuscany | Bergagna S.,Experimental Zooprophylactic Institute of Piemonte | And 9 more authors.
Small Ruminant Research | Year: 2016

Mastitis is the most important disease of dairy small ruminants affecting animal welfare, agricultural economy, and food safety. Only a few investigations on the bacterial epidemiology of udder infections have been performed. Aim of the study was to describe the Italian epidemiology of bacterial mastitis in small ruminant dairy herds. An ad hoc electronic data collection module was created by the National Reference Center for Sheep and Goat Mastitis (C.Re.N.M.O.C). Public health veterinary laboratories of the Experimental Zooprophylactic Institutes (EE.ZZ.II) (n = 10) were selected. Nine (90.0%) EE.ZZ.II. participated to the survey and 8 (87.5%) provided a full report. Bacteriological culture results from 30,232 sheep and goat milk samples collected in 1,795 herds between 2013 and 2014 were analyzed. Coagulase-negative staphylococci (CNS) were the most frequently isolated bacteria in dairy sheep and goats, followed by Staphylococcus aureus; other bacterial species were Pseudomonas spp., Streptococcus uberis, Enterobacteriaceae, Enterococcus spp., Streptococcus spp. and Coryneiforms. Italian results confirm previous findings described in other countries; CNS are the most prevalent bacteria, probably due to subclinical symptoms, whereas Staphyloccocus aureus is the most prevalent clinical mastitis etiological agent. The present survey, based on the first, Italian standardized data electronic collection focused on small ruminant mastitis, may represent the backbone for future control and preventive strategies nationwide. © 2016 The Author(s) Source

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