Experimental biochemistry unit

Saudi Arabia

Experimental biochemistry unit

Saudi Arabia
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Al-Malki A.L.,King Abdulaziz University | Al-Malki A.L.,Bioactive Natural Products Research Group | Barbour E.K.,American University of Beirut | Ea H.,King Abdulaziz University | And 5 more authors.
African journal of traditional, complementary, and alternative medicines : AJTCAM | Year: 2017

BACKGROUND: The goal of this study was identification signaling molecules mediated the formation of AGEs in brain of rats injected with CdCl2 and the role of camel whey proteins and Brassicaceae extract on formation of AGEs in brain.METHODS: Ninety male rats were randomly grouped into five groups; Normal control (GpI) and the other rats (groups II-V) were received a single dose of cadmium chloride i.p (5 μg/kg/b.w) for induction of neurodegeneration. Rats in groups III-V were treated daily with whey protein (1g/kg b.w) or Brassicaceae extract (1mg/kg b.w) or combined respectively for 12 weeks.RESULTS: It was found that whey protein combined with Brassicaceae extract prevented the formation of AGEs and enhance the antioxidant activity compared with untreated group (p <0.001). Serum tumor necrosis factor (TNF-α) and interleukine (IL-6) levels were significantly decreased (p<0.01) in rats treated with whey protein and Brassicaceae extract formation compared with untreated. The combined treatment showed a better impact than individual ones (p<0.001). The level of cAMP but not cGMP were lowered in combined treatment than individual (p<0.01).CONCLUSION: It can be postulated that Whey protein + Brassicaceae extract formation could have potential benefits in the prevention of the onset and progression of neuropathy in patients.


Bawazeer N.A.,Taif University | Choudary H.,King Abdulaziz University | Zamzami M.A.,King Abdulaziz University | Abdulaal W.H.,King Abdulaziz University | And 5 more authors.
African journal of traditional, complementary, and alternative medicines : AJTCAM | Year: 2017

BACKGROUND: High plasma concentration of low-density lipoprotein cholesterol (LDL-c) plays a significant role in the incidence of atherosclerosis and coronary heart diseases (CHD).MATERIALS AND METHODS: The purpose of this study was to investigate the mechanism by which citrus flavonoids, naringenin regulate the LDL receptor (LDLr) gene in human liver using the human hepatoma cell line, HepG2 as a model.RESULTS: Time-course transient transfection of HepG2 cells with luciferase reporter-gene constructs incorporating the promoters of SREBP-1a,-1c, -2 and LDLr, revealed that in lipoprotein-deficient medium (LPDM), only SREBP-1a promoter activity was increased significantly after 4h exposure to 200μM naringenin respectively. However, after 24h incubation with 200μM naringenin the gene expression activities of all the SREBP-1a, -1c, -2 and LDLr promoter-constructs were increased significantly. The effects of both 200μM naringenin on elevating LDLr mRNA are possibly due to regulation of gene transcription by SREBP-la and SREBP-2. However, the suppression effect of 200μM naringenin on hepatic SREBP-1c mRNA expression is likely associated with the reduction in mRNA expression of both acetyl-CoA carboxylase and fatty acid synthase in human hepatoma HepG2 cells. It was found that, 200μM naringenin was likely to stimulate LDLr gene expression via increase phosphorylation of PI3K and ERK1/2 which enhance the transcription factors SREBP-1a and SREBP-2 mRNA levels and increased their protein maturation in human hepatoma HepG2 cell.CONCLUSION: Diets supplemented with naringenin could effectively reduce mortality and morbidity from coronary heart diseases and as cardio-protective effects in humans.


Al-Ghamdi M.A.,King Abdulaziz University | Al-Ghamdi M.A.,Experimental Biochemistry Unit | Choudhry H.,King Abdulaziz University | Al-Doghather H.A.,King Abdulaziz University | And 7 more authors.
African journal of traditional, complementary, and alternative medicines : AJTCAM | Year: 2017

BACKGROUND: Body overweight and obesity were considered as a risk factor for many systemic diseases as diabetic hypertension, cardiovascular diseases, and some cancers. The lipoic acid and Co Q are considered as coenzymes needed for enhancement metabolic rate. The goal of this study is to evaluate the anti-obese effect of lipoic acid alone or combined with Co-Q in rats.MATERIALS AND METHODS: Ninety male albino rats (100-150g) were used in this study, divided into six groups (15 each). Group I: Normal rats fed normal diet. Group II: Rats fed high fat diet (HFD). Group III: Rats fed HFD were given lipoic acid (10 μg/kg b w/day) intra-gastric by stomach tube. Group IV: Rats fed HFD were given Co-Q (10 μg/kg b.w/day) intra-gastric. Group V: Rats fed HFD were given lipoic acid (50 mg/kg b w/day) and Co-Q (10 μg/kg b. w/day). Group VI: Rats were given orlistat intra-gastric (10 mg/kg b w/day) as positive control for 6 weeks. Serum was subjected for determination of lipid profile, liver function tests atherogenic factor and lipoprotein lipase.RESULTS: It was found that treatment with lipoic acid or Co-Q or combined showed increase in the activity of lipoprotein lipase (P < 0.001) and reduction of atherogenic effect and obesity index (P <0.001). The effect of combined gives good results than orlistat or individual treatment.CONCLUSION: lipoic acid combined with Co-Q increase fat oxidation and prevent fat accumulation. The consumption of lipoic acid daily promotes fat oxidation and prevents its accumulation in visceral tissues. Further studies should be carried out to examine the mechanistic signals of these nutrients that helps in weight management.


Omar A.K.,King Abdulaziz University | Omar A.K.,Experimental Biochemistry unit | Ahmed K.A.,King Abdulaziz University | Helmi N.M.,King Abdulaziz University | And 11 more authors.
African Health Sciences | Year: 2017

Background: Blood-related hereditary diseases are widespread in Eastern and SouthWestern regions of Saudi Arabia until recently. In this study, we used Na+, K+ATPase as an enzymatic indicator for the diagnosis of the diseases. Materials and methods: Individuals with different blood diseases (iron deficiency (n=13), anemia (n=14), thalassemia (n=16) and sickle cell anemia (n=12) were studied for Na+, K+-ATPase activity in the plasma membrane of red blood cell and compared with those of the healthy ones (n=20) of the same age and gender living in Jeddah, Saudi Arabia. Results: There was a significant elevation in the specific activity of Na+, K+ATPase in individuals with anemia compared with those of control (0.0094 + 0.001 nmol / mg protein/min versus 0.0061±0.001). On the other hand, there was a significant reduction in enzyme activity in thalassemia (0.0028±0.002 nmol / mg protein/min) and sickle cell anemia cases (0.0042±0.001 nmol / mg protein/min) compared to the control group. The cut off value for Na+, K+ATPase activity is 0.005 εmol Pi/minshowing 94% sensitivity and 93% specificity for the differentiation of blood abnormality. Conclusion: It can be recommended that the activity of Na+, K+-ATPase can be used for the diagnosis of individuals with blood diseases/disorders. © 2017 Makerere University, Medical School. All rights reserved.


Al-Malki A.L.,King Abdulaziz University | Al-Malki A.L.,Bioactive Natural Products Research Group | Al-Malki A.L.,Experimental Biochemistry Unit | Moselhy S.S.,King Abdulaziz University | And 3 more authors.
African Journal of Traditional, Complementary and Alternative Medicines | Year: 2016

Background: The objective of this study was to investigate the fatty acids profiling in diabetic rats induced by sterptozocine (STZ) and their response to administration of lutein and carnitine. Materials and methods: Ninety male albino rats were divided into 6 groups as follows: Normal control. The remaining rats were injected i.p a single dose of STZ (65 mg/kg bw) for induction of diabetes. Diabetic rats were grouped as: GP II: (Untreated): GP III: Rats were given orally with L-lutein (100 mg/kg bw).GP IV: Rats were given carnitine (30 μg/kg) i.p. GP V: Rats were given carnitine and lutein GP VI were given metformin (100mg/kg bw/d) for 6 weeks. Results: Treatment of diabetic rats with lutein, L-carnitine, combined decreased the levels of glucose, HA1C compared with untreated diabetic (p<0.001). Administration of L-lutein, carnitine, combined to normal rats significantly decreased the levels of myristic, palmitice, palmitoleic, stearic, linoleic, α-linolenic, arachidic and eicosadienoic when compared with control normal rats (p<0.001). Conclusion: Abnormalities of fatty acids composition was observed in diabetic rats. Combination treatment with lutein and carnitine could ameliorate deleterious effect induced by STZ and attenuate the changed fatty acid composition © 2016, African Ethnomedicines Network. All rights reserved.


Moselhy S.S.,King Abdulaziz University | Moselhy S.S.,Bioactive Natural Products Research Group | Moselhy S.S.,Experimental Biochemistry Unit | Moselhy S.S.,Ain Shams University | And 3 more authors.
African Journal of Traditional, Complementary and Alternative Medicines | Year: 2016

Background: The current trend globally is the utilization of natural products as therapeutic agents given its minimum side effects. The leaves of Stevia contain several active ingredient compounds such as rebaudioside. Stevia extract have been used for many purposes. Active oxygen radicals can induce base modifications, DNA breakage, and intracellular protein crosslink's. This study was done to evaluate the potential of stevia extract as antibacterial and antioxidants actions. Materials and methods: Antibacterial activity of different extracts of stevia was tested in vitro against different species of bacteria and hepato-protective efficacy was testes in rats injected with CCl4 as hepatotoxic. Results: Acetone extract exhibited antibacterial activity against selected five bacteria species. The acetone extract suppressed the elevation of serum ALT (p <0.05) and AST (p <0.001) activities induced by CCl4. Animals given stevia extract showed prevention against deleterious effects of CCl4 by lowering lipid peroxidation and enhancement of antioxidant activities as SOD and CAT. The protection trial is better than treatment trial. Total phenolic content of aqueous and acetone extracts were found 30 mg and 85 mg gallic/gm extract respectively. While the total flavonoids were 40 mg and 80 mg quercetin/g respectively. The GC-MS analysis showed that monoterpene and indole are the main components. Aqueous extract don’t show any antibacterial activity against the tested strains. The antioxidant properties were attributable to its phenolic content to scavenge free radicals. Conclusion: Acetone extract possess a potent antimicrobial and activity against deleterious effect of CCl4-caused liver damage. © 2016, African Ethnomedicines Network. All rights reserved.


Moselhy S.S.,King Abdulaziz University | Moselhy S.S.,Experimental biochemistry unit | Moselhy S.S.,Ain Shams University | Kamal I.H.,King Abdulaziz University | And 7 more authors.
African Health Sciences | Year: 2016

Objective: Coronary artery diseases including atherosclerosis is considered as commonest problem worldwide. Ergosterols are the main components of vegetable oils and nuts. The objective of this study was to evaluate the potential hypoplipidemic and hypocholesterolemic effects of ergosterol in combination with niacin in rats fed high fat diet (HFD). Methods: Eighty male albino rats were included in this study divided into two main groups: Group I: Normal rats fed standard diet treated with either niacin (8.5 mg /kg b.w) or ergosterol (100 mg/Kg b.w) or both. Group II; rats fed HFD treated with either niacin (8.5 mg /kg b.w) or ergosterol (100 mg/Kg b.w) or both The feeding and treatment lasted for 8 weeks. Results: A significant elevation in the levels of total cholesterol, triacylglycerol, VLDL-c, LDL-c and atherogenic factor (p<0.001) in rats fed on HFD compared with normal control while HDL-c was significantly reduced in HFD rats compared with control group. Supplementation of diet with niacin or ergosterol or combined exerts improvement in the studied parameters by lowering triacylglycerol, total cholesterol, LDL-c and atherogenic factor and elevate HDL-c near to the value of control. Niacin combined with ergosterol were effective in the reduction of hydroxy methyl glutaryl-CoA reducatase (HMGCoA) compared with control (p<0.001). The combined effect was more potent than individual alone. Conclusion: Utilization of niacin and ergosterol may prevent the hypercholesterolemia and incidence of coronary heart diseases. These functional foods act as nutriceutical as dyslipidemics. © 2016, Makerere University, Medical School. All rights reserved.


Khan J.A.,King Abdulaziz University | Moselhy S.S.,King Abdulaziz University | Moselhy S.S.,Experimental biochemistry unit | Moselhy S.S.,Ain Shams University
African Journal of Traditional, Complementary and Alternative Medicines | Year: 2016

Background: Hepatitis is one of the major public health problems worldwide. This study was designed to evaluate the potential hepatoprotective effects of lycopene (Lyco) and selenium (Se) against galactosamine (Gala) induced hepatitis in rats. Materials and Methods: Seventy five (75) male albino rats were grouped into five of fifteen rats each. GP 1: Control. Animals in Groups (II-V) were injected i.p with Gala (300 mg/kg b.w daily) for 5 days. GP III: Rats were orally pretreated with Lyco (15 mg/kg b.w). GP IV: rats were pretreated orally sodium selenite (0.1 mg/kg b.w) by gavages. GP V: Rats treated with both Lyco and Se. The treatment was continuous for 30 days. Results: The levels of serum inflammatory markers interleukine-6 (IL-6), tumor necrosis factor TNF- α, nitric oxide (NO) and malondialdhyde (MDA) were markedly elevated in rats injected with Gala compared with control group. Administration of Lyco combined with Se reversed these effects and significantly reduced the levels of these markers (p<0.001) compared with Gala. In addition, combined treatment resulted in a significant improvement in antioxidant activities as superoxide dismutase (SOD) and catalase compared with untreated. Non-significant changes were recorded in the activities of UDP-glucuronyltransferase and sulphotransferase in rats injected with Gala but the activity of glutathione Stransferase was significantly elevated (p<0.001). Conclusion: The combined effect of Lyco + Se showed a significant hepatoprotective action against Gala induced hepatitis in rats through inhibition of release of inflammatory mediators and enhancement of antioxidant capacity. © 2016, African Ethnomedicines Network. All rights reserved.


Kumosani T.,King Abdulaziz University | Kumosani T.,Experimental Biochemistry Unit | Iyer A.,King Abdulaziz University | Iyer A.,Experimental Biochemistry Unit | And 3 more authors.
Life Science Journal | Year: 2014

The prevalence of obesity is increasing in Saudi Arabia. The search for genes that increase the susceptibility to develop obesity has become important. One set of candidate genes for obesity is the heterotrimeric G proteins, which are key components of intracellular signal transduction and play a focal role in adipogenesis. The aim of this study was to study the association between the C825T (C-to-T substitution at nucleotide 825 in exon 10) (rs5443) polymorphism of the GNB3 gene and obesity in a sample Saudi population. Blood samples from 116 healthy volunteers in age group 18-60 years were taken and obesity status was determined by the Body Mass Index(BMI). DNA was extracted from whole blood and PCR for the GNB gene was done. The PCR product thus obtained was subjected to restriction analysis using the enzyme BsaJ1 to determine the presence or absence of the GNB3 C825T polymorphism. Alleles T represent the absence of restriction site while alleles C indicate the presence of restriction site. Association between the genotypes and obesity was determined. We found that the presence of the T allele was a major contributing factor to obesity because from our study group we observed a higher occurrence of TT genotype in obese and overobese people while the normal people had a high occurrence of CT genotype and the underweight people had a high occurrence of CC genotype. Genotyping studies clearly showed that the T allele was a major contributing factor towards obesity. Higher T allele frequency was associated with obesity as compared with normal individuals.


Iyer A.,King Abdulaziz University | Iyer A.,Experimental Biochemistry Unit | Yaghmoor S.,Experimental Biochemistry Unit | Hagras M.,King Abdulaziz University | And 3 more authors.
Life Science Journal | Year: 2014

The prevalence of obesity is increasing in Saudi Arabia. The search for genes that increase the susceptibility to develop obesity has become important. One set of candidate genes for obesity is the heterotrimeric G proteins, which are key components of intracellular signal transduction and play a focal role in adipogenesis. The aim of this study was to study the association between the C825T (C-to-T substitution at nucleotide 825 in exon 10) (rs5443) polymorphism of the GNB3 gene and obesity in a sample Saudi population. Blood samples from 116 healthy volunteers in age group 18-60 years were taken and obesity status was determined by the Body Mass Index(BMI). DNA was extracted from whole blood and PCR for the GNB gene was done. The PCR product thus obtained was subjected to restriction analysis using the enzyme BsaJ1 to determine the presence or absence of the GNB3 C825T polymorphism. Alleles T represent the absence of restriction site while alleles C indicate the presence of restriction site. Association between the genotypes and obesity was determined. We found that the presence of the T allele was a major contributing factor to obesity because from our study group we observed a higher occurrence of TT genotype in obese and overobese people while the normal people had a high occurrence of CT genotype and the underweight people had a high occurrence of CC genotype. Genotyping studies clearly showed that the T allele was a major contributing factor towards obesity. Higher T allele frequency was associated with obesity as compared with normal individuals.

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