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Khan J.A.,King Abdulaziz University | Moselhy S.S.,King Abdulaziz University | Moselhy S.S.,Experimental biochemistry unit | Moselhy S.S.,Ain Shams University
African Journal of Traditional, Complementary and Alternative Medicines | Year: 2016

Background: Hepatitis is one of the major public health problems worldwide. This study was designed to evaluate the potential hepatoprotective effects of lycopene (Lyco) and selenium (Se) against galactosamine (Gala) induced hepatitis in rats. Materials and Methods: Seventy five (75) male albino rats were grouped into five of fifteen rats each. GP 1: Control. Animals in Groups (II-V) were injected i.p with Gala (300 mg/kg b.w daily) for 5 days. GP III: Rats were orally pretreated with Lyco (15 mg/kg b.w). GP IV: rats were pretreated orally sodium selenite (0.1 mg/kg b.w) by gavages. GP V: Rats treated with both Lyco and Se. The treatment was continuous for 30 days. Results: The levels of serum inflammatory markers interleukine-6 (IL-6), tumor necrosis factor TNF- α, nitric oxide (NO) and malondialdhyde (MDA) were markedly elevated in rats injected with Gala compared with control group. Administration of Lyco combined with Se reversed these effects and significantly reduced the levels of these markers (p<0.001) compared with Gala. In addition, combined treatment resulted in a significant improvement in antioxidant activities as superoxide dismutase (SOD) and catalase compared with untreated. Non-significant changes were recorded in the activities of UDP-glucuronyltransferase and sulphotransferase in rats injected with Gala but the activity of glutathione Stransferase was significantly elevated (p<0.001). Conclusion: The combined effect of Lyco + Se showed a significant hepatoprotective action against Gala induced hepatitis in rats through inhibition of release of inflammatory mediators and enhancement of antioxidant capacity. © 2016, African Ethnomedicines Network. All rights reserved. Source


Iyer A.,King Abdulaziz University | Iyer A.,Experimental biochemistry unit | Yaghmoor S.,Experimental biochemistry unit | Hagras M.,King Abdulaziz University | And 3 more authors.
Life Science Journal | Year: 2014

The prevalence of obesity is increasing in Saudi Arabia. The search for genes that increase the susceptibility to develop obesity has become important. One set of candidate genes for obesity is the heterotrimeric G proteins, which are key components of intracellular signal transduction and play a focal role in adipogenesis. The aim of this study was to study the association between the C825T (C-to-T substitution at nucleotide 825 in exon 10) (rs5443) polymorphism of the GNB3 gene and obesity in a sample Saudi population. Blood samples from 116 healthy volunteers in age group 18-60 years were taken and obesity status was determined by the Body Mass Index(BMI). DNA was extracted from whole blood and PCR for the GNB gene was done. The PCR product thus obtained was subjected to restriction analysis using the enzyme BsaJ1 to determine the presence or absence of the GNB3 C825T polymorphism. Alleles T represent the absence of restriction site while alleles C indicate the presence of restriction site. Association between the genotypes and obesity was determined. We found that the presence of the T allele was a major contributing factor to obesity because from our study group we observed a higher occurrence of TT genotype in obese and overobese people while the normal people had a high occurrence of CT genotype and the underweight people had a high occurrence of CC genotype. Genotyping studies clearly showed that the T allele was a major contributing factor towards obesity. Higher T allele frequency was associated with obesity as compared with normal individuals. Source


Kumosani T.,King Abdulaziz University | Kumosani T.,Experimental biochemistry unit | Iyer A.,King Abdulaziz University | Iyer A.,Experimental biochemistry unit | And 3 more authors.
Life Science Journal | Year: 2014

The prevalence of obesity is increasing in Saudi Arabia. The search for genes that increase the susceptibility to develop obesity has become important. One set of candidate genes for obesity is the heterotrimeric G proteins, which are key components of intracellular signal transduction and play a focal role in adipogenesis. The aim of this study was to study the association between the C825T (C-to-T substitution at nucleotide 825 in exon 10) (rs5443) polymorphism of the GNB3 gene and obesity in a sample Saudi population. Blood samples from 116 healthy volunteers in age group 18-60 years were taken and obesity status was determined by the Body Mass Index(BMI). DNA was extracted from whole blood and PCR for the GNB gene was done. The PCR product thus obtained was subjected to restriction analysis using the enzyme BsaJ1 to determine the presence or absence of the GNB3 C825T polymorphism. Alleles T represent the absence of restriction site while alleles C indicate the presence of restriction site. Association between the genotypes and obesity was determined. We found that the presence of the T allele was a major contributing factor to obesity because from our study group we observed a higher occurrence of TT genotype in obese and overobese people while the normal people had a high occurrence of CT genotype and the underweight people had a high occurrence of CC genotype. Genotyping studies clearly showed that the T allele was a major contributing factor towards obesity. Higher T allele frequency was associated with obesity as compared with normal individuals. Source


Moselhy S.S.,King Abdulaziz University | Moselhy S.S.,Experimental biochemistry unit | Moselhy S.S.,Ain Shams University | Kamal I.H.,King Abdulaziz University | And 7 more authors.
African Health Sciences | Year: 2016

Objective: Coronary artery diseases including atherosclerosis is considered as commonest problem worldwide. Ergosterols are the main components of vegetable oils and nuts. The objective of this study was to evaluate the potential hypoplipidemic and hypocholesterolemic effects of ergosterol in combination with niacin in rats fed high fat diet (HFD). Methods: Eighty male albino rats were included in this study divided into two main groups: Group I: Normal rats fed standard diet treated with either niacin (8.5 mg /kg b.w) or ergosterol (100 mg/Kg b.w) or both. Group II; rats fed HFD treated with either niacin (8.5 mg /kg b.w) or ergosterol (100 mg/Kg b.w) or both The feeding and treatment lasted for 8 weeks. Results: A significant elevation in the levels of total cholesterol, triacylglycerol, VLDL-c, LDL-c and atherogenic factor (p<0.001) in rats fed on HFD compared with normal control while HDL-c was significantly reduced in HFD rats compared with control group. Supplementation of diet with niacin or ergosterol or combined exerts improvement in the studied parameters by lowering triacylglycerol, total cholesterol, LDL-c and atherogenic factor and elevate HDL-c near to the value of control. Niacin combined with ergosterol were effective in the reduction of hydroxy methyl glutaryl-CoA reducatase (HMGCoA) compared with control (p<0.001). The combined effect was more potent than individual alone. Conclusion: Utilization of niacin and ergosterol may prevent the hypercholesterolemia and incidence of coronary heart diseases. These functional foods act as nutriceutical as dyslipidemics. © 2016, Makerere University, Medical School. All rights reserved. Source

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