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Zielinsky P.,Institute of Cardiology of Rio Grande Do Sul FUC | Piccoli Jr. A.L.,Institute of Cardiology of Rio Grande Do Sul FUC | Manica J.L.,Institute of Cardiology of Rio Grande Do Sul FUC | Nicoloso L.H.,Institute of Cardiology of Rio Grande Do Sul FUC | And 10 more authors.
Journal of Perinatology | Year: 2010

Objective: To test the hypothesis that maternal consumption of polyphenol-rich foods during third trimester interferes with fetal ductal dynamics by inhibition of prostaglandin synthesis.Study Design: In a prospective analysis, Doppler ductal velocities and right-to-left ventricular dimensions ratio of 102 fetuses exposed to polyphenol-rich foods (daily estimated maternal consumption >75th percentile, or 1089 mg) were compared with 41 unexposed fetuses (flavonoid ingestion <25th percentile, or 127 mg).Result: In the exposed fetuses, ductal velocities were higher (systolic: 0.96 ± 0.23 m/s; diastolic: 0.17 ± 0.05 m/s) and right-to-left ventricular ratio was higher (1.23 ± 0.23) than in unexposed fetuses (systolic: 0.61 ± 0.18 m/s, P < 0.001; diastolic: 0.11 ± 0.04 m/s, P = 0.011; right-to-left ventricular ratio: 0.94 ± 0.14, P < 0.001).Conclusion: As maternal polyphenol-rich foods intake in late gestation may trigger alterations in fetal ductal dynamics, changes in perinatal dietary orientation are warranted. © 2010 Nature Publishing Group All rights reserved.


Zarogoulidis P.,Aristotle University of Thessaloniki | Hohenforst-Schmidt W.,University of Wurzburg | Huang H.,Shanghai University | Sahpatzidou D.,Experimental Animal Laboratory | And 15 more authors.
Diagnostic pathology | Year: 2014

BACKGROUND: Chronic obstructive pulmonary disease presents with two different phenotypes: chronic bronchitis and emphysema with parenchymal destruction. Decreased expression of vascular endothelial growth factor and increased endothelial cell apoptosis are considered major factors for emphysema. Stem cells have the ability of vascular regeneration and function as a repair mechanism for the damaged endothelial cells. Currently, minimally invasive interventional procedures such as placement of valves, bio-foam or coils are performed in order to improve the disturbed mechanical function in emphysema patients. However, these procedures cannot restore functional lung tissue. Additionally stem cell instillation into the parenchyma has been used in clinical studies aiming to improve overall respiratory function and quality of life.METHODS: In our current experiment we induced emphysema with a DDMC non-viral vector in BALBC mice and simultaneously instilled stem cells testing the hyposthesis that they might have a protective role against the development of emphysema. The mice were divided into four groups: a) control, b) 50.000 cells, c) 75.000 and d) 100.000 cells.RESULTS: Lung pathological findings revealed that all treatment groups had less damage compared to the control group. Additionally, we observed that emphysema lesions were less around vessels in an area of 10 μm.CONCLUSIONS: Our findings indicate that stem cell instillation can have a regenerative role if applied upon a tissue scaffold with vessel around.VIRTUAL SLIDES: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_195.


Hohenforst-Schmidt W.,Aristotle University of Thessaloniki | Zarogoulidis P.,University of Wurzburg | Linsmeier B.,Medinos Clinic Sonneberg | Kioumis I.,University of Wurzburg | And 14 more authors.
Journal of Cancer | Year: 2014

Inhaled therapy for lung cancer is a local form of treatment. Currently inhaled non-specific cytotoxic agents have been evaluated as a future treatment for local disease control and distant metastasis control. There are few information regarding the influence of local transporters and gene expression of the respiratory epithelium to the absorption of administered drugs. In the current work we used adenoviral-type 5(dE1/E3) (Cytomegalovirus promoter) with human ABCA10 transgene (Ad-h-ABCA10) purchased from Vector Labs® in order to investigate whether gene therapy can be used as a pre-treatment to enhance the efficiency of inhaled cisplatin. We included the following groups to our work: a) control, b) aerosol vector, c) aerosol vector plus cisplatin, d) aerosol cisplatin, e) intratumoral cisplatin administration, f) intratumoral vector plus cisplatin administration. The results indicate that the aerosol cisplatin group had a long term survival with the intratumoral cisplatin group following. The enhancement of the ABCA family locally to the respiratory system prior to the aerosol cisplatin administration can be used safely and efficiently. Future treatment design of local therapies should include the investigation of local transporters and genes. © Ivyspring International Publisher.


Baliaka A.,G. Papanikolaou General Hospital | Zarogoulidis P.,Aristotle University of Thessaloniki | Zarogoulidis P.,University of Duisburg - Essen | Domvri K.,Aristotle University of Thessaloniki | And 13 more authors.
Gene Therapy | Year: 2014

Lung cancer still remains to be challenged by novel treatment modalities. Novel locally targeted routes of administration are a methodology to enhance treatment and reduce side effects. Intratumoral gene therapy is a method for local treatment and could be used either in early-stage lung cancer before surgery or at advanced stages as palliative care. Novel non-viral vectors are also in demand for efficient gene transfection to target local cancer tissue and at the same time protect the normal tissue. In the current study, C57BL/6 mice were divided into three groups: (a) control, (b) intravenous and (c) intatumoral gene therapy. The novel 2-Diethylaminoethyl-Dextran Methyl Methacrylate Copolymer Non-Viral Vector (Ryujyu Science Corporation) was conjugated with plasmid pSicop53 from the company Addgene for the first time. The aim of the study was to evaluate the safety and efficacy of targeted gene therapy in a Lewis lung cancer model. Indeed, although the pharmacokinetics of the different administration modalities differs, the intratumoral administration presented increased survival and decreased distant metastasis. Intratumoral gene therapy could be considered as an efficient local therapy for lung cancer. © 2014 Macmillan Publishers Limited.

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