Acton, MA, United States
Acton, MA, United States

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The present invention relates to ion mobility spectrometers. In one embodiment, the ion mobility spectrometer of the present invention uses a simplified ion mobility spectrometer design having helical resistive material to form substantially constant electric fields that guide ion movements. The drift tube for ion mobility spectrometers described herein is constructed with a non-conductive structure. This configuration provides a robust ion mobility spectrometer that is simple to build. One feature of the present invention is that the drift tube design described herein enables the ion mobility spectrometer to be built with a lower weight, lower power consumption, lower manufacturing cost, and free of sealants.


Patent
Excellims Corporation | Date: 2014-03-14

One aspect of the present invention is to extract multiple ionic species in a FAIMS into one or more IMS drift tubes simultaneously. By adjusting FAIMS operational parameters, ions in FAIMS are detected on IMS detectors through separation in FAIMS and/or separation and collection in the IMS. This method provides a continuous separation of specific ions from an ion swarm by employing a high-field differential mobility analyzer (FAIMS) with a plurality of orthogonal transitions paths spaced incrementally along the electrodes which allow additional separation by a conventional IMS drift tube. The components of the sample can be collected or detected.


Patent
Excellims Corporation | Date: 2014-03-14

The present invention uses an AC voltage instead of DC voltage on an ion gate to filter/selectively pass ions. The ions that pass through the AC ion gate can be further separated in a spectrometric instrument. An ion mobility spectrometer using the AC ion gate can achieve better gating performance. For a time of flight ion mobility spectrometer with an AC ion gate, a narrow pulse of selected ions can be passed into a drift tube where they are separated based on their low field ion mobility. Moreover, when the AC voltage at the AC ion gate has a waveform as used for differential ion mobility spectrometry, the time of flight ion mobility spectrometer is converted into a two dimensional separation spectrometer, where ions are first separated based on their high field ion mobility and then further separated based on their low field ion mobility.


Patent
Excellims Corporation | Date: 2013-03-09

The present invention relates to a parallel IMS and MS measurement method where a sample flow is split and delivered to an IMS and a MS in parallel. A parallel acquisition MS/IMS method is used to supplement LC-MS and or MS data by using a synchronized MS/IMS acquisition.


Grant
Agency: National Science Foundation | Branch: | Program: SBIR | Phase: Phase I | Award Amount: 150.00K | Year: 2013

This Small Business Innovation Research (SBIR) Phase I project will develop a prototype gas-phase two-dimensional electrophoresis (GP2DE) instrument for analyzing protein structures in mixtures with less sample and higher throughput compared to traditional two-dimensional gel electrophoresis (2DGE). The instrument leverages the previously developed multi-dimensional ion mobility spectrometry (MDIMS) method for separating gas-phase protein ions based on molecular size and shape in both time and space with high-performance IMS. Understanding proteins' functions will be crucial to achieving the advances required for personalized medicine, which will be a strong driver of the proteomics field both scientifically and economically. Consequently, designing specialized therapies requires intricate knowledge of the complete protein structure. GP2DE will provide a rapid tool for detecting differences in protein structures using coupled ion mobility spectrometry analyses to give 2D gel electrophoresis-type separations in seconds to minutes. Increasing the throughput for analyses of protein structures addresses the inefficiencies in the labor intensive 2DGE process to build the structure knowledge base more rapidly. In particular, tools that can detect post-translational modifications of proteins, such as glycosylation or phosphorylation, will be valuable to proteomics. GP2DE can detect these structural changes easily because the structural differences will result in different ion mobilities. The broader impact/commercial potential of this project, if successful, will provide a flexible, general purpose biomolecule analysis tool for researchers with limited budgets and/or facility space. This would be particularly useful for undergraduate laboratories where the shortened learning curve would allow for more productive research under typical time and resource constraints. The proposed gas-phase two-dimensional electrophoresis (G2DPE) evolves from advanced IMS technologies for structural size and shape analyses that are geared to be user-friendly. The market for proteomics research products has been projected to be several billion dollars in the next five years. GP2DE reduces the need to perform many more time consuming 2DGE analyses that have higher operating costs from labor and supplies, decreasing research costs and adding to laboratory productivity. Therefore, having another powerful tool for elucidating protein structural information will propel the rate of development of the next-generation of biotherapies better tailored to individual physiologies. The research conducted will be disseminated in peer-reviewed scientific journals and at scientific conferences to foster the adoption and advancement of multi-dimensional IMS methods for proteomics.


Patent
Excellims Corporation | Date: 2014-11-10

An ion mobility spectrometry method wherein ions are separated along a drift axis while providing a drift gas flow in a direction that is substantially neither in the direction of the drift axis nor opposite to the drift axis. Ion mobility spectrometer operation methods use a cross-directional gas flow in a drift tube and/or a segmented drift tube for pre-separation.


Patent
Excellims Corporation | Date: 2014-07-22

The present invention relates to a parallel IMS and MS measurement method where a sample flow is split and delivered to an IMS and a MS in parallel. A parallel acquisition MS/IMS method is used to supplement LC-MS and or MS data by using a synchronized MS/IMS acquisition.


Grant
Agency: NSF | Branch: Standard Grant | Program: | Phase: | Award Amount: 150.00K | Year: 2013

This Small Business Innovation Research (SBIR) Phase I project will develop a prototype gas-phase two-dimensional electrophoresis (GP2DE) instrument for analyzing protein structures in mixtures with less sample and higher throughput compared to traditional two-dimensional gel electrophoresis (2DGE). The instrument leverages the previously developed multi-dimensional ion mobility spectrometry (MDIMS) method for separating gas-phase protein ions based on molecular size and shape in both time and space with high-performance IMS. Understanding proteins functions will be crucial to achieving the advances required for personalized medicine, which will be a strong driver of the proteomics field both scientifically and economically. Consequently, designing specialized therapies requires intricate knowledge of the complete protein structure. GP2DE will provide a rapid tool for detecting differences in protein structures using coupled ion mobility spectrometry analyses to give 2D gel electrophoresis-type separations in seconds to minutes. Increasing the throughput for analyses of protein structures addresses the inefficiencies in the labor intensive 2DGE process to build the structure knowledge base more rapidly. In particular, tools that can detect post-translational modifications of proteins, such as glycosylation or phosphorylation, will be valuable to proteomics. GP2DE can detect these structural changes easily because the structural differences will result in different ion mobilities.

The broader impact/commercial potential of this project, if successful, will provide a flexible, general purpose biomolecule analysis tool for researchers with limited budgets and/or facility space. This would be particularly useful for undergraduate laboratories where the shortened learning curve would allow for more productive research under typical time and resource constraints. The proposed gas-phase two-dimensional electrophoresis (G2DPE) evolves from advanced IMS technologies for structural size and shape analyses that are geared to be user-friendly. The market for proteomics research products has been projected to be several billion dollars in the next five years. GP2DE reduces the need to perform many more time consuming 2DGE analyses that have higher operating costs from labor and supplies, decreasing research costs and adding to laboratory productivity. Therefore, having another powerful tool for elucidating protein structural information will propel the rate of development of the next-generation of biotherapies better tailored to individual physiologies. The research conducted will be disseminated in peer-reviewed scientific journals and at scientific conferences to foster the adoption and advancement of multi-dimensional IMS methods for proteomics.


Grant
Agency: Department of Health and Human Services | Branch: | Program: SBIR | Phase: Phase I | Award Amount: 170.65K | Year: 2013

DESCRIPTION: The ultimate goal of the Phase I SBIR project is to establish the feasibility of using ion mobility spectrometry (IMS) or liquid chromatography-IMS (LC-IMS) as a fast, selective and sensitive method for rapid monitoring of bio manufacturing processes for on-line use to improve the understanding of the process and provide real-time feedback. Excellims will develop the LC-IMS method for monitoring the most important chemical species that inform the process monitors. Bio manufacturers are limitedby current real-time monitoring tools and must determine quality after the product is completed. Therefore, successful development of the method will fulfill the need for tools that increase the efficiency of the entire process that will translate into operational and cost savings for mass producing biologics. PUBLIC HEALTH RELEVANCE PUBLIC HEALTH RELEVANCE: Ion Mobility Spectrometry (IMS) has been developed as a powerful separation and analysis method for organic and biological samples thatcan be used for monitoring at a field location. Combining the advantages of IMS in speed and simplicity will enable a new application of the technique for real-time, on-site monitoring at a biotherapeutic manufacturing facility. Development of the new application for bio manufacturing process monitoring will provide innovative methods for improving the efficiency of the process controls and for determining the production end-points.


Patent
Excellims Corporation | Date: 2016-01-11

The present invention uses an AC voltage instead of DC voltage on an ion gate to filter/selectively pass ions. The ions that pass through the AC ion gate can be further separated in a spectrometric instrument. An ion mobility spectrometer using the AC ion gate can achieve better gating performance. For a time of flight ion mobility spectrometer with an AC ion gate, a narrow pulse of selected ions can be passed into a drift tube where they are separated based on their low field ion mobility. Moreover, when the AC voltage at the AC ion gate has a waveform as used for differential ion mobility spectrometry, the time of flight ion mobility spectrometer is converted into a two dimensional separation spectrometer, where ions are first separated based on their high field ion mobility and then further separated based on their low field ion mobility.

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